Biomolecules
·
2021
·Vol. 11
·期 8
·1133
·
IF 4.879
Kevin Adi Kurnia,Fiorency Santoso,Bonifasius Putera Sampurna,Gilbert Audira,Jong-Chin Huang,Kelvin H.-C. Chen,Chung-Der Hsiao
Tail coiling is a reflection response in fish embryos that can be used as a model for neurotoxic analysis. The previous method to analyze fish tail coiling is largely based on third-party software. In this study, we aim to develop a simple and cost-effective method called TCMacro by using ImageJ macro to reduce the operational complexity. The basic principle of the current method is based on the dynamic change of pixel intensity in the region of interest (ROI). When the fish tail is moving, the average intensity is increasing. In time when the fish freeze, the peak of mean intensity is maintaining at a relatively low level. By using the optimized macro settings and excel VBA scripts, all the tail coiling measurement processes can be archived with few operation steps with high precision. Three major endpoints of tail coiling counts, tail coiling duration and tail coiling intervals can be obtained in batch. To validate this established method, we tested the potential neurotoxic activity of Tricaine (methanesulfonate, MS-222) and psychoactive compound of caffeine. Zebrafish embryos after Tricaine exposure displayed significantly less tail coiling activity in a dose-dependent manner, and were comparable to manual counting through the Wilcoxon test and Pearson correlation double validation. Zebrafish embryos after caffeine exposure displayed significantly high tail coiling activity. In conclusion, the TCMacro method presented in this study provides a simple and robust method that is able to measure the relative tail coiling activities in zebrafish embryos in a high-throughput manner.Keywords:ImageJ;chorion;tail coiling;ROI;zebrafish;embryo
研究领域:
发育生物学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
Food and Bioprocess Technology
·
2024
·
IF 5.3
Hu Jin,Yang Wencheng,Lan Huanling,Lin Xueyuan,Li Chang
Rice ( Oryza sativa ) bran is a by-product of rice processing, which contains rich nutrients and is a resource that can be fully utilized. However, lipase in rice bran can lead to rancidity of rice bran oil. Therefore, it is important to study the characteristics of rice bran lipase for the stable storage of rice bran. A new lipase from rice bran was isolated by DEAE Sepharose column and gel filtration column, respectively. The determination of lipase’s molecular weight and purity was conducted utilizing the SDS-PAGE technique, and the enzyme was identified through LC-MS/MS. Concurrently, an investigation was conducted on the characteristics of the lipase. The findings indicated that the lipase had a molecular weight of 36.352 kDa. Optimal catalytic activity for rice bran lipase (RBL) was observed at a temperature of 40 ℃ and pH value of 7.0, while it exhibited remarkable stability below 20 ℃ and within the pH range from 6.0 to 8.0. The stability of the RBL against various metal ions and high NaCl concentrations was observed to be relatively modest. Notably, the catalytic activity was significantly influenced by certain organic solvents (such as acetone, THF, and trichloromethane) as well as several inhibitors ( P < 0.05). Furthermore, RBL demonstrated a preference for hydrolyzing long-chain p-NP esters (C12-C16).
研究领域:
生物化学
·
食品科学
✓ 已验证
DOI
高影响因子
Reproductive Biology
·
2024
·
IF 2.5
Romualdo Sciorio,Pier Francesco Greco,Mohammed Adel,Lucia Maresca,Ermanno Greco,Steven Fleming
In the last decades, to enhance success rates in assisted reproductive technology (ART) cycles, scientists have continually tried to optimize embryo culture and selection to increase clinical outcomes. In this scenario, the application of laser technology has increased considerably worldwide and is currently applied across ART in several ways: for assisted hatching (AH) or thinning of the zona pellucida (ZP), embryo biopsy, to immobilize and select the sperm during intracytoplasmic sperm injection, as well as to induce artificial blastocyst shrinkage before cryopreservation. Laser-AH has been suggested as a procedure to improve embryo implantation: the concept is that drilling holes through or thinning of the ZP could improve the hatching process and implantation. The artificial disruption of the ZP can be performed by different approaches: mechanically, chemically and with the laser, which is one of the most favourable and easy methods to remove part of the ZP and to augment the possibilities of implantation in patients defined as having a poor prognosis of success, or when the ZP is too thick. However, in the current literature, there is not sufficient evidence about the potential risk or impairment that laser utilization might induce on embryo development; therefore, the main aim of the current review is to provide an overview of the existing knowledge on the ZP and the mechanisms of manipulating it to improve the effectiveness of ART. Also, it emphasizes the positive aspect of laser application as a powerful tool that might increase the chance of pregnancy for infertile couples undergoing ART cycles.
研究领域:
生殖生物学
·
辅助生殖技术
·
胚胎学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Xiaoyong Huang,Zhaoyang Yan,Kui Zhu,Shuangyang Ding
Ionizable strategies are routinely used to enhance the solubility and dissolution rates of various pharmaceuticals. These chemicals may directly affect aquatic environment once discharged from factories, hospitals or livestock farms. Here, we assessed the potential side effect of tetracyclines (TCs) on the development of zebrafish embryos. Tetracycline hydrochloride decreased water pH from 6.4 to 4.4 at 30 mg/L. Acidified water exceeded the tolerance of zebrafish embryos in pure water during the early ten hours post fertilization (hpf). Interestingly, we found that Ca 2+ in the embryo medium could increase the tolerance of embryos to acidified water. Furthermore, we found that the protection of Ca 2+ was not due to the formation of TCs-Ca 2+ complexes under acidic condition, based on spectral analysis. Meanwhile we showed that exogenous addition of Ca 2+ could inhibit the accumulation of Ca 2+ from the cytoplasm to the surface of embryos. These results may shed light on the strategies for protecting aquatic animals from acidic environments.
研究领域:
水生毒理学
·
环境科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
·
2023
·Vol. 59
·期 1
·1-13
·
IF 2.6
Xiao Xu,Zhang Chunling,Liu Yang,Wang Xiaofei,You Chunxiang
Agrobacterium-mediated genetic transformation is inefficient in apple, which limits the development of apple-related research and industry . Using molecular methods to create a new and more efficient method of genetic transformation has become the focus of research, and improving the efficiency of the three key steps of transformation, regeneration, and rooting has become an effective means of achieving this goal. Baby Boom (BBM) is widely used in plant biotechnology as an important transcription factor regulating adventitious shoot regeneration and somatic embryogenesis. A homologous gene, MDP0000125317 , with high amino acid sequence similarity to AtBBM was cloned in this study, and it was identified from the Royal Gala apple genome ( Malus × domestica Borkh.), which was named as MdBBM . The qRT-PCR analysis revealed that MdBBM was expressed at higher levels in the ovary, roots, and seeds. Agrobacterium -mediated transformation of pRI101- MdBBM significantly improved the shoot regeneration efficiency in apples. In the experiment, 33 of the MdBBM-OE transformants were obtained. Compared with the control, only adventitious shoots were formed on the regeneration shoot medium. In vitro leaves of MdBBM-OE transformants were regenerated in three forms: adventitious shoots, adventitious roots, and somatic embryos. Overexpression of MdBBM promoted somatic embryogenesis under 2,4-dichlorophenoxyacetic acid (2,4-D) induction.
研究领域:
植物学
·
遗传学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
THERIOGENOLOGY
·
2024
·
IF 2.4
Xiu-Li Zhang,Zhi-Yong An,Gao-Jie Lu,Tuo Zhang,Cheng-Wei Liu,Meng-Qi Liu,Qing-Xin Wei,Lin-Hu Quan,Jin-Dan Kang
Oocytes and embryos are highly sensitive to environmental stress in vivo and in vitro. During in vitro culture, many stressful conditions can affect embryo quality and viability, leading to adverse clinical outcomes such as abortion and congenital abnormalities. In this study, we found that valeric acid (VA) increased the mitochondrial membrane potential and ATP content, decreased the level of reactive oxygen species that the mitochondria generate, and thus improved mitochondrial function during early embryonic development in pigs. VA decreased expression of the autophagy-related factors LC3B and BECLIN1 . Interestingly, VA inhibited expression of autophagy-associated phosphorylation-adenosine monophosphate-activated protein kinase (p-AMPK), phosphorylation-UNC-51-like autophagy-activated kinase 1 (p-ULK1, Ser555), and ATG13, which reduced apoptosis. Short-chain fatty acids (SCFAs) can signal through G-protein-coupled receptors on the cell membrane or enter the cell directly through transporters. We further show that the monocarboxylate transporter 1 (MCT1) was necessary for the effects of VA on embryo quality, which provides a new molecular perspective of the pathway by which SCFAs affect embryos. Importantly, VA significantly inhibited the AMPK-ULK1 autophagic signaling pathway through MCT1, decreased apoptosis, increased expression of embryonic pluripotency genes, and improved embryo quality.
研究领域:
生殖生物学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
REPRODUCTIVE TOXICOLOGY
·
2024
·
IF 3.3
Hanwen Zhang,Yu Li,Na Li,Yilong Miao,Shaochen Sun,Ling Gu,Bo Xiong
Perfluorooctanoic acid (PFOA) exposure severely affects the health of animals and humans, including early embryonic development, but the effective approaches to improve the quality of embryos exposed to PFOA have not been explored. Here, we report that nicotinamide mononucleotide (NMN) can be used to attenuate the impairment of mouse early embryos caused by PFOA exposure. We find that NMN supplementation maintains the normal spindle assembly and proper chromosome alignment by restoring the acetylation level of microtubule to enhance the mitotic capacity of embryos at zygotic cleavage stage under PFOA exposure. In addition, NMN exerts its beneficial effect by enhancing mitochondrial function and eliminating accumulated reactive oxygen species (ROS), which in turn alleviates DNA damage and apoptosis in PFOA-exposed 2-cell embryos. Moreover, NMN ameliorates the quality of PFOA-exposed blastocysts via recovering the octamer-binding transcription factor 4 (Oct4) expression, the actin dynamics, and the total number of cells. Collectively, our findings demonstrate that supplementation with NMN is a feasible strategy to restore the compromised early embryonic development under PFOA exposure, providing a scientific basis for application of NMN to increase the female fertility.
研究领域:
生殖生物学
·
毒理学
·
发育生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomicrofluidics
·
2015
·Vol. 9
·期 2
·
IF 3.357
Li Yinbao,Yang Xiujuan,Chen Zuanguang,Zhang Beibei,Pan Jianbin,Li Xinchun,Yang Fan,Sun Duanping
Investigations were conducted to determine acute effects of Pb 2+ and Cu 2+ presented individually and collectively on zebrafish embryos. Aquatic safety testing requires a cheap, fast, and highly efficient platform for real-time evaluation of single and mixture of metal toxicity. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic effects of Pb 2+ and Cu 2+ using zebrafish ( Danio rerio ) embryos. The microfluidic chip is composed of a disc-shaped concentration gradient generator and 24 culture chambers, which can generate one blank solution, seven mixture concentrations, and eight single concentrations for each metal solution, thus enabling the assessment of zebrafish embryos. To test the accuracy of this new chip platform, we have examined the toxicity and teratogenicity of Pb 2+ and Cu 2+ on embryos. The individual and combined impact of Pb 2+ and Cu 2+ on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators, such as spontaneous motion at 22 hours post fertilization (hpf), mortality at 24 hpf, heartbeat and body length at 96 hpf, etc. It was found that Pb 2+ or Cu 2+ could induce deformity and cardiovascular toxicity in zebrafish embryos and the mixture could induce more severe toxicity. This chip is a multiplexed testing apparatus that allows for the examination of toxicity and teratogenicity for substances and it also can be used as a potentially cost-effective and rapid aquatic safety assessment tool.
研究领域:
毒理学
·
环境科学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Halah Aalim,Tarun Belwal,Lei Jiang,Hao Huang,Xianghe Meng,Zisheng Luo
The purpose of this investigation was to explore aqueous glycerol as food grade solvent for rice bran polyphenolic antioxidants extraction and investigate the antidiabetic and antiglycation potential of the extract and its major polyphenols. Response surface methodology (Box-Behnken design) was implemented to optimize experimental conditions for rice bran polyphenols and antioxidants extraction. The optimum conditions provided a very satisfactory outcome in total polyphenols (549.6 ± 8.05 mg gallic acid equivalent per 100 g dry weight) and DPPH scavenging activity (57.83 ± 1.8%). The chromatographic separation of the extract showed higher concentrations of ferulic acid, ρ -coumaric acid and chlorogenic acid followed by caffeic acid, syringic acid and rutin. Moreover, the extract inhibited α-amylase, α-glucosidase, and lipase activity as 34.2 ± 3.2%, 29.9 ± 1.0%, and 12.4 ± 2.3%, respectively. Furthermore, fructose readily forms Advanced Glycation End Products (AGEs) fluorescence with basic amino acids in low concentration in simulated intestinal digestion conditions. The extract inhibited AGEs formation by 52.6% at 20 μL/mL. Our study indicated that the antidiabetic and antiglycation potential of rice bran extract was mainly driven by the extract polyphenols. Rice bran glycerol extract could be employed as a natural antidiabetic and antiglycation remedy, and as economically and eco-friendly source of potent bioactive compounds with satisfactory outcome.
研究领域:
食品科学
·
植物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Lei Shang,Xiao Wang,Wei Zhang,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Huai-Sheng Wang
Ratiometric electrochemiluminescence (ECL) assays have attracted widespread attentions in biosensing owing to their precise measurements by eliminating the environmental interferences. However, they mostly needed two eligible luminophors, increasing the complexity of the systems and limiting their practical applications. Herein, using luminol as single luminophor, a dual-potential ratiometric ECL strategy was proposed to detect carcinoembryonic antigen (CEA). The luminol exhibited cathodic and anodic emissions on graphene-ionic liquid-platinum (GR-IL-Pt) composites and Ti 3 C 2 MXenes-Au NPs hybrids, respectively. Then, a sandwich ECL sensor was fabricated using GR-IL-Pt composites as matrix to immobilize the primary antibodies of CEA and Ti 3 C 2 MXenes-Au NPs hybrids as platform to load the secondary antibodies. With the presence of CEA, the ratio of anodic ECL to cathodic ECL (ECL anodic /ECL cathodic ) increased obviously, realizing sensitive ratiometric detection of CEA. In addition, the ECL anodic /ECL cathodic was independent with the concentrations of H 2 O 2 , greatly improving the test reliability. The developed ECL sensor exhibited a sensitive detection toward CEA, performing a wide linearity in the range of 0.1 pg mL −1 - 10 ng mL −1 with a low detection limit of 34.58 fg mL -1 (S/N = 3). Furthermore, this strategy exhibited a good practicality to detect CEA in human serums, providing a promising strategy in ECL bioanalysis.
研究领域:
生物传感
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Yange Luan,Qijie Ma,Yihong Hu,Xinyi Hu,Guanyu Chen,Jiaru Zhang,Zhong Li,Dongdong Xie,Guanghui Ren,Yunyi Yang,Guifu Ding,Kate Fox,Bao Yue Zhang,Jian Zhen Ou
2D nanomaterials have shown significant advances in next-generation biosensor development. However, current 2D-enabled electronic biosensors rarely achieve a detection limit down to pico-gram level, possibly due to their intrinsic low carrier mobilities, biocompatibility or instability. TiO 2 , a biocompatible material, has been widely adopted in bio-applications. Recently, 2D TiO 2 with a unique planar hexagonal phase exhibits a reduced bandgap of 2 eV and high p-type mobility. A small perturbation could stimulate an observable electronic swing, enabling a high-performance biosensor in a label-free manner. Here, 2D h-TiO 2 is fabricated into a liquid-gated field-effect transistor, while a wide spectrum, nonspecific biomarker carcinoembryonic antigen (CEA) is selected as the target molecule. The h-TiO 2 surface is first functionalized with 3-aminopropyltriethoxysilane and subsequently decorated with anti-CEA, creating an affinity probe toward CEA molecules. The real-time response to different concentrations of CEA is tested from 1 pg mL −1 to 10 ng mL −1 under a low working voltage of 0.05 V, and an ultralow detection limit of 0.22 pg mL −1 is achieved. Meanwhile, the selectivity of the devices is demonstrated using cytokeratin-19-fragment and neuron-specific enolase. The results highlight the potential of layered hexagonal metal oxides for high-performance biosensors, enabling future low-power portable devices for disease diagnosis.
研究领域:
纳米材料
·
生物传感器
·
电子器件
·
生物医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Xueke Liu,Chang Liu,Peng Wang,Yiran Liang,Jing Zhan,Zhiqiang Zhou,Donghui Liu
Alpha-cypermethrin (Alpha-CP), an important pyrethroid pesticide , has been widely used for pest control in agriculture and parasite control in livestock farms. Thus, alpha-CP is easily exposed to wild birds and poultry, which may pose a potential risk to birds. Alpha-CP and its metabolites have been detected in many environmental samples, including poultry and wild birds. We studied the distribution and metabolism of alpha-CP and its metabolites in embryo development and newborn chick. The results showed that metabolites were the main residual forms of alpha-CP in different stages of life and might increase the exposure risk of bird and its offspring . Metabolomics investigation of newborn chick exhibited that the metabolic profiles of chicks were disturbed, especially lipid metabolism . The concentrations of cis-DCCA and trans-DCCA were high in the first and second weeks of chick growth, indicating that chicks have limited ability to further metabolize and excrete cis-DCCA and trans-DCCA during the early stages of chicks. Toxicokinetics of alpha-CP in adult hens showed that alpha-CP was rapidly metabolized to acid metabolites, which could be further metabolized and excreted. The results about metabolism of alpha-CP in different stages of chicken indicate that the ability of the embryo and early chick to metabolize alpha-CP and its metabolites was the weakest. Therefore, it is of important significance to focus on evaluating the ecological risk of cypermethrin on birds at different stages of life cycle.
研究领域:
毒理学
·
生态学
·
动物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）