SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Qian Wang,Huanshun Yin,Yunlei Zhou,Jun Wang,Shiyun Ai
As an important epigenetic modification, 5-formylcytosine (5fC) plays a vital role in cell differentiation, genomic DNA structure and function regulation. Its low abundance in all suckler tissues and cells and high structural similarity with cytosine (C) and its derivatives, the sensitive and selective detection method for 5fC is required. In this work, a photoelectrochemical biosensor was constructed using BiIO 4 -WS 2 composite as photosensitive material, the synergy of ternary heterojunction of BiIO 4 -WS 2 /CuO as signal amplification technology and 5-formylcytosine deoxyribonucleoside (5fdC) as target. Firstly, the ITO electrode modified by BiIO 4 -WS 2 was aminated by (3- aminopropyl) triethoxysilane. 5fC was captured on the electrode surface with amidogen as recognition reagent, based on the amide bond formed between amidogen and aldehyde group. Then, 4-carboxyphenylboronic acid (4-CPBA) was linked by covalent reaction between boronic acid and O -diol of 5fdC. Finally, the signal amplification material of amino-functionalized CuO was deposited on the electrode surface through the formation of amide bond based on −COOH of 4-CPBA and –NH 2 on CuO. In view of the constitution of a ternary heterojunction between CuO and BiIO 4 -WS 2 , the photoactivity of BiIO 4 -WS 2 was greatly enhanced, which also increased the detection sensitivity with the wide examination area from 0.005 to 200 nM and low limit of detection was 0.38pM (3σ). Based on the specific covalent identification pattern, this method showed high selectivity for 5fC, even discriminating 5fC with C and 5hmC. The impacts of antibiotics and heavy metals on 5fdC content change in genomic DNA of wheat tissues were investigated, and thus the feasibility of this work was evaluated.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 27
·11704-11709
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IF 3.288
Chao Fan,Hao Tang,Licheng Wang,Yijing Li,Xusheng Wang,Shuai Wang,Xiaojing Liang
Using N-isopropylacrylamide (NIPAM) as a monomer, a core–shell material was successfully prepared via in situ polymerization on the surface of silica microspheres (named NIPAM@Sil), which acts as a hydrophilic interaction chromatography (HILIC) stationary phase. A method for the in situ polymerization of polymer monomers on a silica surface has been developed. It was shown using scanning electron microscopy (SEM) that NIPAM is evenly distributed on the surface of the silica. The synthesized NIPAM@Sil possesses a polar acylamino group arising from the polymerization of NIPAM. As demonstrated by the hydrophilicity, NIPAM@Sil showed excellent HILIC performance for the separation of hydrophilic compounds. This method provides a simple approach for the preparation of a polymer coated silica core–shell stationary phase.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Chao Fan,Bei Liu,Hui Li,Kaijun Quan,Jia Chen,Hongdeng Qiu
In this work, three new mixed-mode stationary phases were prepared, based on different ratio of N-vinyl pyrrolidone (NVP) copolymerized together with undecylenic acid (UA) on silica microspheres surface without silanization, which named Sil@NVPUA series. The combination of NVP and UA rendered the Sil@NVPUA suitable for reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC), and shown excellent methyl, planar, isomers and ion selectivity. Five types of model analytes including eight polycyclic aromatic hydrocarbons, six alkylbenzenes, eight nucleosides and nucleobases, seven ginsenosides and five oxazolidinones can be well separated on this stationary phase. The preparation method of NVP and UA modified silica-based stationary phase is simple, and it also provides a new idea for the design of synthetic polymers to develop mixed-mode chromatography.
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PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 22
·4119
·
IF 3.06
Zhonghua Dong,Yueyue Sun,Guangwei Wei,Siying Li,Zhongxi Zhao
Cordyceps Sinensis, a traditional Chinese medicine and a healthy food, has been used for the treatment of kidney disease for a long time. The aim of present study was to isolate a nucleoside/nucleobase-rich extract fromCordyceps Sinensis(CS-N), determine the contents of nucleosides and nucleobases, and explore its anti-diabetic nephropathy activity. CS-N was isolated and purified by using microporous resin and glucan columns and the unknown compounds were identified by using HPLC-DAD and LC-MS. The effects of CS-N on the epithelial–mesenchymal transition (EMT), extracellular matrix (ECM) depositions, and the MAPK signaling pathway were evaluated in streptozotocin (STZ)-induced diabetic mice and high glucose (HG)-exposed HK-2 cells. CS-N significantly attenuated the abnormity of renal functional parameters, ameliorated histopathological changes, and inhibited EMT and ECM accumulation by regulating p38/ERK signaling pathways. Our findings indicate that CS-N exerts a therapeutic effect on experimental diabetic renal fibrosis by mitigating the EMT and the subsequent ECM deposition with inhibition of p38 and ERK signaling pathways.Keywords:nucleosides and nucleobases;diabetic nephropathy;HK-2 cells;extracellular matrix;epithelial–mesenchymal transition
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Tengda Wang,Jianxing Shan,Li Wang,Xiangwen Zhang,Guozhu Li
Aqueous-phase hydrogenation of 4-nitrophenol (4-NIP) by H 2 has been investigated in the presence of Pd nanoparticles (NPs) regulated by small biological molecules. Nucleosides and their derivatives were employed as capping agents of Pd NPs, and their effect on Pd activity was evaluated. All four kinds of nucleosides exhibited obvious promoting effect on the activity of Pd NPs in comparison to that without any capping agent. The general promoting effect sequence of nucleosides and their derivatives on the activity of Pd NPs was nucleoside ≥ nucleotide > deoxynucleoside > base > deoxynucleotide. The quantitative correlation between the activity promoting effect and the structure of nucleosides and their derivatives were also studied using the method based on poly-parameter linear free energy relationships. Moreover, the kinetic behaviors of 4-NIP hydrogenation over Pd NPs regulated by different nucleosides (adenosine, guanosine, cytidine, and β-thymidine) were studied systematically and different kinetic models were developed to fit the data. The obtained kinetic data fitted well to the Langmuir-Hinshelwood (L-H) model, where the surface reaction is the rate-controlling step. The apparent activation energy, adsorption enthalpy of 4-NIP, and dissociated adsorption enthalpy of hydrogen were calculated and compared for a better understanding of the mechanism. Pd NPs regulated by cytidine showed the lowest apparent activation energy of 43.66 kJ mol −1 .
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JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Tianpei Cai,Haijuan Zhang,Jia Chen,Zhan Li,Hongdeng Qiu
A new strategy based on synergistic effect of the carbon dots and their precursor was proposed to enhance the selectivity of hydrophilic interaction liquid chromatography (HILIC). In this work, polyethyleneimine (PEI) and PEI-functionalized carbon dots (PEICDs) mix-grafted silica packing material was prepared to act as a novel HILIC stationary phase. Both inner and outer surface of the porous silica are decorated with the mixture of PEI and carbon dots. This stationary phase, namely Sil-PEI/CDs, demonstrate enhanced retention ability and selectivity toward polar analytes, with which 11 nucleosides and nucleobases and 9 ginsenosides can be nicely separated. The Sil-PEI/CDs (RSD 0.12% – 0.54%) exhibited even better stability than the traditional PEI modified stationary phase (RSD 0.39% – 0.87%) within 40 h of continuously elution. And excellent column efficiency was observed from Sil-PEI/CDs (∼65,000 plates/m for cytidine). The strategy of mixed stabilization revealed a new method to prepare good performanced carbon dots based chromatographic column.
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高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Chaozhan Wang,Huanhuan Xu,Yinmao Wei
Boronate affinity adsorption is uniquely selective for cis-diol-containing molecules. The preparation and application of boronate affinity materials has attracted much attention in recent years. In this work, a high-capacity boronate affinity adsorbent was prepared by surface-initiated reversible addition–fragmentation chain transfer polymerization (SI-RAFT). Commercial aminated poly(glycidyl methacrylate) (PGMA) microspheres were modified with the chain transfer agent (CTA) S-1-dodecyl-S-(α,α-dimethyl-α-acetic acid)trithiocarbonate (DDATC). Boronate-affinity adsorbents were then prepared via SI-RAFT polymerization employing 3-acrylamidophenylboronic acid (AAPBA) as the monomer . The Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption and desorption measurements have proven the successful grafting of AAPBA on PGMA microspheres surface. The boronate affinity adsorbents thus prepared possess much higher adsorption capacity (99.2 µmol/g of adenosine) and both faster adsorption and desorption speed towards ribonucleosides , the adsorption and desorption could be completed in 2 min. The high selectivity of the adsorbents to ribonucleosides was verified in the presence of a large excess of deoxynucleosides. The boronate affinity adsorbents were then employed for sample pretreatment before HPLC analysis of ribonucleosides in serum. The ribonucleosides were effectively enriched by boronate affinity dispersive solid-phase extraction (BA-DSPE), with high mass recoveries and good precision. The simultaneous determination of uridine and guanosine in calf serum was achieved by utilizing the standard addition method, their contents were determined to be 170 ± 11.6 ng/mL and 39.6 ± 4.4 ng/mL respectively. The results proved that the prepared boronate affinity materials could be applied for sample pretreatment of cis-diol containing molecules in biological samples.
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高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2015
·Vol. 120
·期 1
·1009-1025
·
IF 2.042
Wang Xue-Jie,You Jin-Zong
The thermal decomposition processes for ten pyrimidine nucleoside analogs were measured with thermogravimetry and differential scanning calorimetry. The IR spectra, high-performance liquid chromatography, and liquid chromatography–mass spectrometry of pyrimidine nucleoside analogs and their residues of thermal decomposition at various temperatures were determined. The molecular bond orders of pyrimidines and pyrimidine nucleoside analogs were calculated with an ab initio method from the GAMESS program. We then discuss mechanisms of thermal decomposition in these pyrimidine nucleoside analogs. The results indicate that there are four types of mechanisms. The decomposition mechanism depends on the relative strength of the peptide bond and the amide bond within pyrimidine ring and whether or not accompanied by oxidation reaction. The substituent groups affect the thermal stability and the thermal decomposition mechanism of pyrimidine nucleoside analogs. Increasing the number of electron-donating groups on the pyrimidine ring and furan ring will enhance the peptide bond, and will elevate the temperature of thermal decomposition. There is a positive correlation between the molecular bond orders calculated by quantum chemistry and the thermal decomposition temperature of pyrimidine nucleoside analogs. The stronger the weakest bond order, the higher the decomposition temperature. The molecular bond orders thus can be used as a basis to judge molecular thermal stability for analog compounds with similar molecular structure, size, and energy.
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高影响因子
TALANTA
·
2015
·
IF 3.545
Qing Wang,Yao Long,Lin Yao,Li Xu,Zhi-Guo Shi,Lanying Xu
A mixed-mode chromatographic stationary phase , C18-DTT (dithiothreitol) silica (SiO 2 ) was prepared through “thiol-ene” click chemistry. The obtained material was characterized by fourier transform infrared spectroscope, nitrogen adsorption analysis and contact angle analysis. Chromatographic performance of the C18-DTT was systemically evaluated by studying the effect of acetonitrile content, pH, buffer concentration of the mobile phase and column temperature. It was demonstrated that the novel stationary phase possessed reversed phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode property. The stop-flow test revealed that C18-DTT exhibited excellent compatibility with 100% aqueous mobile phase. Additionally, the stability and column-to-column reproducibility of the C18-DTT material were satisfactory, with relative standard deviations of retention factor of the tested analytes (verapamil, fenbufen , guanine , tetrandrine and nicotinic acid) in the range of 1.82–3.72% and 0.85–1.93%, respectively. Finally, the application of C18-DTT column was demonstrated in the separation of non-steroidal anti-inflammatory drugs, aromatic carboxylic acids , alkaloids, nucleo-analytes and polycyclic aromatic hydrocarbons . It had great resolving power in the analysis of various compounds in HILIC and RPLC chromatographic conditions and was a promising RPLC/HILIC mixed-mode stationary phase.
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高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2629-2636
·
IF 4.58
Cai Tianpei,Zhang Haijuan,Rahman A. F. M. Mustafizur,Shi Yan-Ping,Qiu Hongdeng
Carbon dots derivatized from N-(β-aminoethyl)-γ-aminopropyl-methyldimethoxysilane (AEAPMS) were coated onto silica microparticles. These particles (Sil-CDs) are shown to be an excellent stationary phase for use in hydrophilic interaction chromatography. Analytes including sulfonamides, nucleosides and bases, flavones and amino acids can be well separated on this stationary phase. Compared to a silica stationary phase functionalized with AEAPMS only, the Sil-CDs show enhanced separation performance. The selectivity factors of three nucleosides and bases (1.02–1.09) and four sulfonamides (1.04–1.11) on AEAPMS functionalized silica stationary phase were improved to 1.10–1.20 and 1.13–1.15 respectively on Sil-CDs stationary phase. This is attributed to the higher number of surface functional groups due to the introduction of carbon dots. The successful application of the Sil-CDs stationary phase highlights the potential of carbon dots as a modified material in chromatography.
Schematic presentation of the preparation of silanized carbon dots coated onto silica microparticles. The material represents a new stationary phase for hydrophilic interaction chromatography. It shows improved separation performance compared to a silane-only functionalized silica stationary phase.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jinlei Yang,Jing Wang,Runsong Cheng,Tianyi Liao,Siyuan Pan,Murong Du,Weiliang Liu,Liying Yan,Sichun Zhang,Xinrong Zhang
Single-cell metabolic analysis has not yet achieved the coverage of bulk analysis due to the diversity of cellular metabolites and the ionization competition among species. Direct ionization methods without separation lead to the masking of low-intensity species. By designing a capillary column emitter and introducing reverse-phase chromatography principles, we achieved the microseparation of lipophilic and hydrophilic metabolites and lowered the limit of detection of hydrophilic metabolites to the level of a single oocyte. We identified 517 metabolite species in a single oocyte, achieving coverage and reproducibility comparable to those of bulk analysis. By comparing oocytes at different maturation stages, 76 metabolic features were identified with significant differences between the germinal vesicle and meiosis II stages. Metabolite level changes suggested the roles of lipid metabolism remodeling, increased amino acid synthesis, and a shift from pyrimidine metabolism to purine metabolism in the process of oocyte maturation. This microseparation mass spectrometry analysis is expected to promote single-cell metabolomics.
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Tong Ren,Zhen Feng,Fang Jiang,Xingtong Liu,Tingting Chen,Yanli Guo,Lei Tian,Xiao-Feng Kang,Fujun Yao
Guanine and its derivatives play fundamental roles in many biological processes and their abnormal changes are closely related to diseases. Thus, accurate and sensitive detection of them is beneficial for the early diagnosis and clinical medicine. Herein, a novel nanopore-based sensor is proposed to detection guanine and its derivatives by using a vacancy G-quadruplex (vG4) probe. In this design, the incomplete G-tetrad in vG4 probe can be filled in by guanine derivatives through Hoogsteen hydrogen bond interactions, resulting in stabilization of the G-quadruplex. Based on the different electronic signals between vG4 probe and its binding complexes, the nanopore sensor was able to achieve the detection of six guanine derivatives, including 2΄-dG, Guanine, Guanosine, GMP, GDP and GTP. To demonstrate the analytical performance of the method, a guanine analogue drug was determined as model analyte. The detection limit of acyclovir was 0.0061 μM (3 σ / k ), with a linear range of 0.01 μM – 0.1 μM. This sensing strategy offers a new pathway for label-free detection of nucleobases and derivatives.
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