APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2017
·Vol. 83
·期 10
·
IF 3.807
Lei Yu,Ming-yue Cao,Peng-tao Wang,Shi Wang,Ying-rong Yue,Wen-duo Yuan,Wei-chuan Qiao,Fei Wang,Xin Song
Biohydrogen production from the pulp and paper effluent containing rich lignocellulosic material could be achieved by the fermentation process. Xylose, an important hemicellulose hydrolysis product, is used less efficiently as a substrate for biohydrogen production. Moreover, azo dyes are usually added to fabricate anticounterfeiting paper, which further increases the complexity of wastewater. This study reports that xylose could serve as the sole carbon source for a pure culture of Klebsiella oxytoca GS-4-08 to achieve simultaneous decolorization and biohydrogen production. With 2 g liter−1 of xylose as the substrate, a maximum xylose utilization rate (URxyl) and a hydrogen molar yield (HMY) of 93.99% and 0.259 mol of H2 mol of xylose−1, respectively, were obtained. Biohydrogen kinetics and electron equivalent (e− equiv) balance calculations indicated that methyl red (MR) penetrates and intracellularly inhibits both the pentose phosphate pathway and pyruvate fermentation pathway, while methyl orange (MO) acted independently of the glycolysis and biohydrogen pathway. The data demonstrate that biohydrogen pathways in the presence of azo dyes with sulfonate and carboxyl groups were different, but the azo dyes could be completely reduced during the biohydrogen production period in the presence of MO or MR. The feasibility of hydrogen production from industrial pulp and paper effluent by the strain if the xylose is sufficient was also proved and was not affected by toxic substances which usually exist in such wastewater, except for chlorophenol. This study offers a promising energy-recycling strategy for treating pulp and paper wastewaters, especially for those containing azo dyes.IMPORTANCE The pulp and paper industry is a major industry in many developing countries, and the global market of pulp and paper wastewater treatment is expected to increase by 60% between 2012 and 2020. Such wastewater contains large amounts of refractory contaminants, such as lignin, whose reclamation is considered economically crucial and environmentally friendly. Furthermore, azo dyes are usually added in order to fabricate anticounterfeiting paper, which further increases the complexity of the pulp and paper wastewater. This work may offer a better understanding of biohydrogen production from xylose in the presence of azo dyes and provide a promising energy-recycling method for treating pulp and paper wastewater, especially for those containing azo dyes.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Biotechnology Journal
·
2024
·Vol. 19
·期 9
·2400383
·
IF 3.2
Kaifeng Xu,Ying Huo,Shiming Tang,Shuangyan Han,Ying Lin,Suiping Zheng
Laccases are extensively used in the textile industry due to their ability to decolorize dyes, modify fabric surfaces, and bleach textiles. Identifying a laccase with both high thermal stability and alkali tolerance suitable for textile applications presents a significant challenge. A novel alkaline laccase, LacCT, was discovered from Caldalkalibacillus thermarum and successfully expressed it in Escherichia coli . LacCT displayed optimal activity at 65°C and maintained high stability across a pH range of 6.0–10.0, with an optimal pH of 7.5. Through rational design, the thermal stability of the best variant, G190P/Q254Y/G336M/D510F (LacCT-11), was significantly enhanced, resulting in a half-life of 63.2 min at 60°C – 1.8 times longer than that of the wild type. This research introduces a promising new laccase with considerable potential for decolorizing textile wastewater and improving the ramie degumming process.
研究领域:
酶学
·
生物技术
·
纺织科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
·
2023
·
IF 4.8
Linna Du,Huan Wu,Gang Li,Yi Wei,Fei Wang,Ligen Xu,Xinjiao Dong
Microbial degradation of dyes is fundamental and essential for understanding their environmental fate . In this study, a new bacterial strain with efficient degrading capability of triphenylmethane (TPM) dyes named WKD was isolated and identified as the genus Serratia sp. by 16S rDNA analysis . The decolorization of TPM dyes by the strain especially under extreme environmental conditions were studied. The results showed that the strain could completely decolorize Malachite Green (MG) and Crystal Violet (CV) at pH values ranging from 3.0 to 10.0 and at temperatures ranging from 20 °C to 45 °C in a 12 h of incubation. The strain still showed an excellent decolorizing capability even at low (5 °C) and high (60 °C) temperatures. Moreover, the enzymatic analysis indicated that MnP, NADH-DCIP reductase, MG reductase, and CV reductase might be involved in TPM dyes biodegradation. Besides, the biodegradation pathways were proposed based on UV–visible, FTIR , and LC-Q-TOF-MS analyses. Results indicated that degradation of MG by the strain was mainly regulated by N-demethylation and hydroxylation pathways, while degradation of CV was only by the N-demethylation pathway. Therefore, Serratia sp. strain WKD is expected to have a broad application prospect for the bioremediation of TPM dyes, especially in extreme environments.
研究领域:
微生物学
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环境科学
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产品关联
DOI
高影响因子
Processes
·
2023
·Vol. 11
·期 3
·903
·
IF 3.5
Yajie Wang,Xin Dong,Chengfeng Liu,Peng Cheng,Gilles Mailhot
The decolorization of azo dye Orange II using a UVA-Fe3+-PMS-oxalate system was studied. A series of experiments was performed to investigate the effects of several variables, including the pH, PMS dosage, Fe3+concentration, oxalate concentration, and coexisting anions. The results revealed that a lower pH facilitated the decolorization, and relatively high decolorization efficiency (97.5%) could be achieved within 5 min at pH 3.0. The electron paramagnetic resonance (ESR) and radical quenching experiments revealed that SO4•−played a crucial role in the decolorization of Orange II (85.8%),•OH was of secondary importance (9%), and1O2made a small contribution to the decolorization (5.2%). Furthermore, the formation of•OH in the experimental system strongly depended on HO2•/O2•−. These reactive oxidants were able to directly attack the azo bond of the luminescent group in Orange II and initiate the decolorization process. The efficient UVA-Fe3+-PMS-oxalate system showed great application potential in the treatment of wastewater contaminated by azo dyes.Keywords:PMS activation;Orange II;decolorization;UVA irradiation;Fe3+-oxalate complexes
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Weijie Liu,Liang Liu,Cong Liu,Yan Hao,Hao Yang,Bo Yuan,Jihong Jiang
Azo dyes are recalcitrant pollutants that constitute a significant burden to the environment. This study focused on decoloring azo dye Congo red using Shewanella oneidensis MR-1 and exploring methylene blue as electron shuttle to enhance its decolorization and detoxication efficiency. The effects of physicochemical parameters on anaerobic decolorization were investigated. The results showed that the suitable pH, temperature and NaCl concentration range for decolorization were 6.0–8.5, 28–40 °C and 0–20 g/L, respectively. Under the optimal conditions, 99.25% decolorization of up to 2 g/L Congo red was achieved within 24 h. Nitrite, nitrate and oxygen could significantly inhibit the decolorization. Riboflavin and methylene blue were found to be able to significantly accelerate decolorization capacity of S. oneidensis MR-1, however, block of Mtr respiratory pathway depressed the promotion effects of riboflavin and methylene blue, suggesting that the promotion effect was achieved by accelerating electron transfer between cells and dyes. Furthermore, the phytotoxicity analysis revealed that the toxicity of Congo red to Triticum aestivum , Pogostemon cablin and Isatis indigotica Fort was decreased during the decolorization process.
研究领域:
环境生物学
·
微生物学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Lijun Shao,Yue Sun,Jin Liang,Meiqing Li,Xueling Li
Different decolorization methods have different effects on the properties of polysaccharides . In this study, polysaccharides extracted from Thesium chinense Turcz ( T. chinense ) were decolorized using either hydrogen peroxide (H 2 O 2 ), which has been previously reported, or activated carbon. To begin, the conditions for decolorization by activated carbon were optimized through single factor experiments and orthogonal modeling and were predicted to be best at 0.5% (w/w) activated carbon, pH 5.0 and 40 °C in a water bath for 60 min. Following treatment by the two methods, the structures of the decolorized polysaccharides were compared. The samples treated by activated carbon (CTP) had higher molecular weight (Mw: 3.064 × 10 5 g/mol) and more sulfate groups (14.33%, w/w), whereas the samples treated by H 2 O 2 (HTP) had lower Mw (8.349 × 10 4 g/mol) and more uronic acid (35.02%, w/w). The antioxidant capacities of the two samples were analyzed by free radical scavenging (DPPH, hydroxyl and ABTS) and cell antioxidant activity (CAA). The HTP showed a better scavenging effect on free radicals and a higher CAA. Together, the results showed that various decolorization methods differentially influence the characteristics of the polysaccharides extracted from T. chinense .
研究领域:
植物化学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2019
·
IF 5.108
Kangmeng Zhu,Xisong Wang,Dong Chen,Wei Ren,Heng Lin,Hui Zhang
Wood-based biochar, as a metal-free heterogeneous activator of peroxydisulfate (PDS), was successfully prepared by pyrolysis of polar sawdust for efficient removal of Acid Orange 7 (AO7). The results demonstrate PDS could be effectively activated by wood-based biochar, and AO7 was rapidly eliminated in a wide range of pH value (3.0–10.0) with AO7 removal achieved ≥ 99.3% after 14 min reaction. The dominant reactive species in the biochar/PDS system were verified via radical quenching tests and electron paramagnetic resonance (EPR) technique. It is speculated that sulfate radicals (SO 4 •− ) and hydroxyl radicals ( • OH) were formed on the surface of biochar. Based on the results of X-ray photoelectron spectroscopy (XPS), π-electron density and oxygen-containing functional groups (especially C–OH) on biochar surface were active centers for the catalytic reaction. Recycle experiments of biochar for 4 runs were carried out and the regeneration method of the catalyst was also studied.
研究领域:
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
Advanced Membranes
·
2021
·
IF 0
Lulu Liu,Hao Zhang,Xiangrong Chen,Yinhua Wan,Jianquan Luo
Ubiquitous membrane fouling is a knotty problem in membrane-based sugar-making process, which limits productivity and sugar quality. Especially during decolorization of sugarcane juice by nanofiltration (NF), fouling mechanism is still obscure and the fouling formation is difficult to control effectively. Herein, based on the analysis of the real fouling layer residue, seven components are served as model solutes and foulants to study their NF separation performance and fouling behavior. We found that the coexistence of polysaccharides and proteins had no synergistic enhancement on membrane fouling, yet attenuated fouling layer because the formation of large complex produced more shear-enhanced back diffusion. Chlorogenic acid (CA), as a phenolic pigment and main pore foulant, aggravates the polysaccharides or proteins fouling owing to its bridging effect between membrane and large foulants, especially polysaccharides with high hydrophilicity. Laccase can catalyze oxidation and self-polymerization of CA, not only reducing its accessibility to block the pores and the bridging effect, but also in-situ forming a hydrophilic coating layer on the membrane surface, which is regarded as an effective pretreatment strategy for fouling control. During the cross-flow filtration of real sugarcane juice, the average permeate flux increases by 67.1% and irreversible fouling decreases by 21.2%, showing great application potential due to its biocompatibility and considerable economic benefits. This work not only elaborately elucidates the fouling mechanism among components in cane juice decolorization by NF, but also proposes an innovative approach to reconstitute fouling layer for improving membrane antifouling performance in practical application.
研究领域:
食品科学
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膜分离技术
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糖业加工
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生物工程
✓ 已验证
DOI
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2016
·
IF 1.207
Tao Lu,Qilei Zhang,Shanjing Yao
In order to improve the efficient decolorization of dye-containing water by biosorbent and understand the biosorption mechanism, the self-immobilization mycelial pellets were prepared using a marine-derived fungus Aspergillus niger ZJUBE-1, and an azo dye, Congo red was chosen as a model dye to investigate batch decolorization efficiency by pellets. The pellets as biosorbent showed strong salt and acid tolerance in biosorption process. The results for dye adsorption showed that the biosorption process fitted well with models of pseudo-second-order kinetic and Langmuir isotherm, with a maximum adsorption capacity of 263.2 mg·g − 1 mycelium. During 6 batches of continuous decolorization operation, the mycelial pellets could possess efficient decolorization abilities (> 98.5%). The appearance of new peak in the UV–Vis spectral result indicated that the decolorization process may also contain biodegradation. The mechanism studies showed that efficient biosorption ability of pellets only relies on the active zone on the surface of the pellet, which can be enhanced by nutrition supplement or be shifted outward by a re-culture process.
研究领域:
环境科学
·
微生物学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry A
·
2015
·Vol. 3
·期 27
·14438-14444
·
IF 7.443
Wen-Ze Qiu,Hao-Cheng Yang,Ling-Shu Wan,Zhi-Kang Xu
Mussel-inspired chemistry has been broadly exploited for multifunctional coatings in the surface modification of applied materials. Polyphenols are ubiquitous in plant tissues and far less expensive than polydopamine for mussel-inspired chemistry. Herein, we report a facile and effective method to modify porous membranes via the co-deposition of catechol (CCh) and polyethyleneimine (PEI). The membrane structures and properties were investigated by ATR/FTIR, XPS, FESEM, zeta potential, water contact angle and pure water flux measurements. The results reveal that the membranes deposited with a CCh–PEI mass ratio of 1 : 0.25 show excellent hydrophilicity, ultrahigh water permeation flux and distinguished surface charges. These membranes were used to decolorize anionic dye solutions during filtration with superior removal efficiencies of over 99%. Moreover, they have good reusability over repeated operations with a simple regeneration process.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2018
·Vol. 41
·期 4
·511-518
·
IF 2.139
Ning Cheng,Qingyun Li,Aixing Tang,Wei Su,Youyan Liu
Aspergillus flavus : fungal strain A5p1 is reported here to decolorize a variety of dyes under broad environmental conditions. For the 15 dyes tested, the decolorization efficiencies ranged from 61.7 to 100.0% at an initial concentration of 100 mg/L. Direct Blue 71 (DB71), Direct Blue 86 (DB86), and Reactive Blue 19 (RB19) were selected as models for comparing decolorization performance. The results show that biosorption and biodegradation work together in the strain to remove the pigments; the effect of biosorption was stronger for DB71 and DB86, whereas the effect of biodegradation was stronger for RB19. For DB71 and DB86, the decolorization rate surpassed 90% with inactivated biomass under acidic conditions and with living biomass under alkaline conditions. DB19 achieved the highest removal rate of 90% under neutral conditions. The strain could effectively decolorize high concentrations of dyes up to 1000 mg/L, which was achieved mainly via biosorption at concentrations below 500 mg/L and via biodegradation at concentrations above 500 mg/L. The findings suggest that A5p1 has a strong adaptability to different dye types and environmental conditions and can, therefore, be potentially used in biological processes for the treatment of dye-containing wastewater.
研究领域:
环境科学
·
微生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2019
·
IF 2.584
Chen Liang,Yan Wang,Yangdong Hu,Lianying Wu,Weitao Zhang
Butyl levulinate (BL) is a versatile chemical utilized widely in food and chemical industries, the production of which by using cellulose in biomass resources is of great significance to its sustainable development. Traditional synthesis processes for n-butyl levulinate are confronted with various problems such as high cost of raw materials, difficulty in separating products, etc. In this paper, a novel process for the preparation of BL from cellulose is proposed. The process is composed of five main unit operations including fed-batch hydrolysis, decolorization, extraction, esterification and purification. A 171.63 g/L concentration of the intermediate levulinic acid was obtained at the fifth feeding through the fed-batch hydrolysis process. The resin-activated carbon secondary decolorization method was adopted to remove the soluble humin byproducts with an accumulative decolorization rate of 89%. In the extraction process, the product BL was chosen as the extractant to avoid the introduction of new impurities. After purification, the purity of the final product BL reaches up to 98 wt %. The proposed technique allows for cost-effective and eco-friendly production of BL from biomass resources.
研究领域:
生物化学工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）