Biomed Research International
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2017
·Vol. 2017
·期 1
·2564363
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IF 2.476
Zuoyi Yang,Junhui Zhou,Yanbin Xu,Yaping Zhang,Haien Luo,KenLin Chang,Yujie Wang
Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human and ecological environment. In this study, a Gram-negative bacterial strain of eliminating indole was isolated from a coking wastewater. The strain was confirmed as Acinetobacter pittii L1 based on the physiological and biochemical characterization and 16S ribosomal DNA (rDNA) gene sequence homology. 400 mg/L indole could be completely removed within 48 h by the strain on the optimum condition of 37°C, pH 7.4, and 150 rpm. The organic nitrogen was converted to NH 3 -N and then to and the organic carbon was partially transferred to CO 2 during the indole biodegradation. The metabolic pathways were proposed to explain the indole degradation based on the liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of indigo, 4-(3-Hydroxy-1H-pyrrol-2-yl)-2-oxo-but-3-enoic acid, and isatin. The toxicity of the biodegradation products was evaluated using the Microtox test, which revealed that the metabolites were more toxic than indole. Our research holds promise for the potential application of Acinetobacter pittii L1 for NHCs degradation, production of indigoids, and soil remediation as well as treatment of indole containing wastewater.
研究领域:
环境微生物学
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生物降解
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分析化学
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环境毒理学
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废水生物处理
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分子生物学
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DOI
高影响因子
Food Analytical Methods
·
2015
·Vol. 9
·期 7
·2026-2035
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IF 1.956
Du Jiaojiao,Gao Ruixia,Mu Hui
Phthalates represent a potential risk for humans, since they are ubiquitous environmental contaminants. Efficient extraction and purification procedures are demanded for the detection of low concentration levels of phthalates. In this work, a novel type of molecularly imprinted polymers coated onto the surface of vinyl functionalized multi-walled carbon nanotubes was synthesized and coupled with gas chromatography–mass spectrometry (GC/MS) for the selective separation and determination of dioctyl phthalate (DOP) in beverage samples. The morphology, structure property, and thermostability of the resultant polymers were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The adsorption properties of the prepared polymers were investigated by equilibrium rebinding and competitive experiments. The resultant imprinted nanomaterials exhibited high capacity and favorable selectivity. In addition, the feasibility of the developed method using the obtained imprinted polymers as a solid-phase sorbent coupled with GC/MS for the selective isolation and determination of DOP in different beverage samples was demonstrated. Under optimal conditions, the limit of detection of the proposed method for DOP was 2.3 ng L −1 . DOP spiked at three levels of concentration in beverage samples was extracted and determined through the application of the present method, with recoveries ranging from 88.6 to 93.0 % with relative standard deviations less than 5.6 %.
研究领域:
分析化学
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环境毒理学
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食品安全
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分子识别
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材料科学
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色谱分析
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DOI
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2019
·
IF 1.267
Yue Yang,Zihui Liu,Hao Chen,Shengqing Li
A simple and fast method of low-density solvent based dispersive liquid-liquid microextraction (LDS-DLLME) followed by high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) has been developed for the speciation analysis of organoarsenic and inorganic arsenic in water samples. The low-density solvent (octanol) was given as the organic phase and injected into the aqueous sample (donor phase) with methanol as the disperser. With As (V), As (III), p-APAA, 4-HPAA, ROX and PAA as target species, factors of LDS-DLLME including pH value, anionic carriers, elution conditions and extractant, were studied in detail. Besides, volumes of solvents were further optimised by response surface methodology. Under the optimal conditions, the limits of detection for four phenylarsenics and arsenate were in the range of 0.001–0.039 μg L−1. The relative standard deviations (RSDs) were 3.6–9.4% and the enrichment factors varied from 6.2 to 70.8. The proposed method of LDS-DLLME-HPLC-ICP-MS was satisfactorily applied to the determination of six arsenic compounds in water samples with recoveries of 81.8–111.7% for the spiked lake water samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
高影响因子
CHROMATOGRAPHIA
·
2018
·Vol. 82
·期 3
·683-693
·
IF 1.401
Shen Chen,Wu Tong,Zang Xiaohuan
In this work, a zeolitic imidazolate framework-based graphene oxide composite (ZIF-8@GO) was prepared and used as the adsorbent in hollow fiber stir bar sorptive extraction. The stir bar was constructed of a small section of hollow fiber filled with 5 mg ZIF-8@GO and a thin stainless wire of 1.5 cm long in its lumen, and used to extract the trace levels of phthalate esters from children’s food samples prior to gas chromatography–mass spectrometric detection. The ZIF-8@GO showed a high adsorption efficiency for the compounds containing aromatic rings. The main experimental parameters that can impact the extraction efficiency including extraction temperature, extraction time, stirring rate, sample salinity, and desorption conditions were investigated. Under the optimized conditions, the limits of detection (LODs) for the five phthalate esters (diethyl phthalate, dipropyl phthalate, diisobutyl phthalate, butylbenzyl phthalate, and di(2-ethylhexyl) phthalate) were 0.15–0.6 µg L −1 for CiCi beverage and pear juice samples, and 0.75–2.2 µg kg −1 for jam samples. The limits of quantitation (LOQs) were 0.5–2.0 µg L −1 for CiCi beverage and pear juice samples, and 2.5–7.5 µg kg −1 for jam samples. Wide linear ranges of 0.5–500.0 µg L −1 and 2.5–2000 µg kg −1 were obtained for CiCi beverage, pear juice samples and jam samples, respectively, with good repeatability (RSD < 11%) and high method recoveries (72%–100%). The lifetime test results showed that the ZIF-8@GO-based hollow fiber stir bar can be reused for 120 extraction cycles without a significant loss of extraction efficiency, indicating its high durability and stability. The established method was applied successfully for the determination of the phthalate esters (PAEs) in children’s food samples. Graphical abstract
研究领域:
分析化学
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食品安全
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环境毒理学
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暴露科学
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食品化学
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污染物检测
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DOI
高影响因子
Journal of Analysis and Testing
·
2018
·Vol. 2
·期 4
·332-341
·
IF 0
Wei Yin Hui,Li Xin Yu,Gao Jie,Liu Jia Jun,Yuan Dan,Yin Bin Cheng,Wang Jian
As an antibiotic, tetracycline has been widely used against bacterial infection or as feed additives for growth promotion, which may lead to the enrichment of antibiotic residues in animals or their products. Therefore, the detection of tetracycline is closely related to human health. The morphological transformation of CoOOH nanoflakes induced by tetracycline was monitored by scanning electron microscope images, dark-field light scattering images and optical spectra, which found that tetracycline could decompose CoOOH nanoflakes into small nanoparticles with low light scattering signal. As a result, the reduced light scattering of CoOOH nanoflakes was proportional to the increased concentration of tetracycline in the range of 1.0–250 μmol/L, and the limit of determination (LOD) was 0.23 μmol/L (3σ/k). Most foreign substances did not interfere in the analysis of tetracycline. Furthermore, the concentration of tetracycline in different milk samples detected with the standard addition method was so low that it accorded with the safety regulation, which was similar to the detection result with high-performance liquid chromatography (HPLC).
研究领域:
分析化学
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纳米技术
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食品安全
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药物分析
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产品关联
DOI
Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
·
2019
·Vol. 29
·期 1
·46-53
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IF 1.754
Peng Wei,Han Ping,Yu Luyao,Chen Ying,Ye Bingzhu,Qin Luping,Xin Hailiang,Han Ting
The fruits of Xanthium strumarium L., Asteraceae, have been used for various diseases in Chinese folk medicine, including allergic rhinitis, tympanitis, arthritis, ozena etc. The current study was aimed to investigate the therapeutic effects of caffeoylquinic acids from fruits of X. strumarium on allergic rhinitis in animals. The toxicity test indicated that the caffeoylquinic acids have no obvious toxicity. By using HPLC assays combined with reference standards, ten caffeoylquinic acids were identified as the predominant constituents. Anti-allergic activities of the caffeoylquinic acids were evaluated using passive cutaneous anaphylaxis test and Schultz-Dale test; dimethylbenzene induced ear edema test was performed to evaluate its anti-inflammatory effect. Then, the allergic rhinitis model in rats was established to evaluate the therapeutic effects of the caffeoylquinic acids against allergic rhinitis with the following indexes: allergic rhinitis symptom scores, serum levels of pro-inflammatory cytokines, histopathological examination, and histamine release. Our study revealed that the caffeoylquinic acids showed obvious anti-allergic and anti-inflammatory properties, and its treatments were beneficial for ameliorating the nasal symptoms, decreasing pro-inflammatory cytokines, and inhibiting the releases of histamine. Collectively, the caffeoylquinic acids might be utilized as effective and safe disease therapeutic agents for allergic rhinitis.
研究领域:
药理学
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免疫学
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过敏
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植物化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）