Analytical Methods
·
2018
·Vol. 10
·期 41
·4978-4984
·
IF 2.073
Xing Huang,Kaipeng Zhang,Yanli Tong,Yongsheng Zhong,Zuanguang Chen
In this study, a gradient reverse phase high performance liquid chromatography combined with a capacitively coupled contactless conductivity detection (RP-HPLC-C4D) method for simultaneous determination of phospholipid and fatty glyceride in liposome was developed. After saponification, the fatty glyceride and two types of phospholipids were transformed into octanoic acid, palmitic acid and erucic acid, respectively, which were then separated and detected by RP-HPLC-C4D. The results showed good linearity in the range of 20–1000 μg mL−1 for octanoic acid and erucic acid and 10–1000 μg mL−1 for palmitic acid. The LODs of octanoic acid and palmitic acid reached 2.5 μg mL−1, and 10 μg mL−1 for erucic acid. The average recoveries of octanoic acid, palmitic acid and erucic acid were 100.2%, 102.9% and 101.4% (n = 9), respectively. The developed method could be effectively applied in the quality control and stability monitoring of liposome.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Yang Shu,Weiwei Xue,Xiaoying Xu,Zhimin Jia,Xiaojun Yao,Shuwen Liu,Lihong Liu
Overconsumption of erucic acid has been shown to cause heart damage in animals. The aim of this study is to evaluate the binding behaviour between erucic acid and bovine serum albumin using multi-spectroscopic methods and a molecular docking technique under physiological conditions. We find that erucic acid can quench the intrinsic fluorescence of BSA by dynamic quenching and there is a single class of binding site on BSA. In addition, the thermodynamic functions Δ H and Δ S are 119.14 kJ mol −1 and 488.89 J mol −1 K −1 , indicating that the hydrophobic force is a main acting force. Furthermore, the protein secondary structure changes with an increase in the content of α -helix, measured using synchronous fluorescence, circular dichroism and Fourier transform infrared spectroscopies. The molecular docking results illustrate that erucic acid can bind with the subdomain IIA of the BSA, and hydrogen bonding is also an acting force.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2017
·
IF 2.714
Pan Wang,Jialin Tan,Shuai Pei,Junfeng Wang,Yan Zhang,Xiaoli Sun,Jun Zhang
The formation of wormlike micelles in mixed anionic 2-( N -erucacyl- N -methyl amido) acetate (EMAA) and cationic trioctylmethylammonium bromide (OTAB) surfactants in aqueous solutions and their rheological properties have been investigated. The variety of viscosity depending on the molar ratio has been explained and related to the lengths of wormlike micelle. Molecular dynamics simulation results suggest that the spherical micelles fusion is a crucial stage for the formation of wormlike micelle. The change of solvent accessible surface area (SASA) and the Zeta potential depending on the molar ratio leads to the different barrier height of micelle fusion, and further induces the different lengths of wormlike micelle. Our studies have lighted new views on the understanding of the self-assembly of catanionic surfactants mixture.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Donghui Gao,Huishan Chen,Zhikun Huang,Shishi Pang,Feng Jiang
The limited effectiveness of emulsifiers with suboptimal emulsification capabilities and low reactivity constrains their use in creating porous polymers through the high internal phase emulsion templating method. In this research, we synthesized a piperazinyl-based emulsifier (EA/AEP) featuring acylamino, secondary, and tertiary amines as its hydrophilic components, through a straightforward amidation process. This emulsifier was subsequently employed to stabilize HIPEs and to fabricated porous polymers. In comparison to Span 80, the use of EA/AEP enabled the formation of HIPEs with superior viscosity and stability at the internal phase volume fractions with between 75% and 95% by incorporating 25% EA/AEP. Scanning Electron Microscopy (SEM) revealed the polymers’ well-defined structures and an interconnected network of pore throats. The improved mechanical properties and oil absorbency of the polymers confirmed their structural robustness. Our approach to developing EA/AEP offers valuable insights for designing innovative emulsifiers, while the inclusion of reactive groups in its structure opens up avenues for further chemical modifications.
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-23
·
IF 14.7
Yue Renye,Li Zhe,Liu Huiyi,Wang Youjuan,Li Yuhang,Yin Rui,Yin Baoli,Qian Haisheng,Kang Heemin,Zhang Xiaobing,Song Guosheng
Companion diagnostics using biomarkers have gained prominence in guiding radiotherapy. However, biopsy-based techniques fail to account for real-time variations in target response and tumor heterogeneity. Herein, we design an activated afterglow/MRI probe as a companion diagnostics tool for dynamically assessing biomarker apurinic/apyrimidinic endonuclease 1(APE1) during radiotherapy in vivo. We employ ultrabright afterglow nanoparticles and ultrasmall FeMnO x nanoparticles as dual contrast agents, significantly broadening signal change range and enhancing the sensitivity of APE1 imaging (limit of detection: 0.0092 U/mL in afterglow imaging and 0.16 U/mL in MRI). We devise longitudinally and transversely subtraction-enhanced imaging (L&T-SEI) strategy to markedly enhance MRI contrast and signal-to-noise ratio between tumor and normal tissue of living female mice. The combined afterglow and MRI facilitate both anatomical and functional imaging of APE1 activity. This probe enables correlation of afterglow and MRI signals with APE1 expression, radiation dosage, intratumor ROS, and DNA damage, enabling early prediction of radiotherapy outcomes (as early as 3 h), significantly preceding tumor size reduction (6 days). By monitoring APE1 levels, this probe allows for early and sensitive detection of liver organ injury, outperforming histopathological analysis. Furthermore, MRI evaluates APE1 expression in radiation-induced abscopal effects provides insights into underlying mechanisms, and supports the development of treatment protocols.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 2
·834-850
·
IF 3.2
Jun Wang,Bocheng Wei,Jing Xu,Han Jiang,Yifei Xu,Chuyan Wang
Lactic acid fermentation is an effective method for improving the quality of black chokeberry. This study aimed to investigate the influence of lactic acid bacteria on the phenolic profile, antioxidant activities, and volatiles of black chokeberry juice. Initially, 10 7 cfu/mL of Lactiplantibacillus plantarum , Lactobacillus acidophilus , and Lacticaseibacillus rhamnosus were inoculated into pasteurized black chokeberry juice and fermented for 48 h at 37°C. All these strains enhanced the total phenolic and total flavonoid contents, with La. acidophilus showing the highest total phenolic (1683.64 mg/L) and total flavonoid (659.27 mg/L) contents. Phenolic acids, flavonoids, and anthocyanins were identified using ultrahigh-performance liquid chromatography–tandem mass spectrometry. The prevalent phenolic acid, flavonoid, and anthocyanin in the lactic-acid-fermented black chokeberry juice were cinnamic acid, rutin, and cyanidin-3- O -rutinoside, respectively. Furthermore, following fermentation, the DPPH and ABTS scavenging capacity, as well as the reducing power capacity, increased from 59.98% to 92.70%, 83.06% to 94.95%, and 1.24 to 1.82, respectively. Pearson's correlation analysis revealed that the transformation of phenolic acids, flavonoids, and anthocyanins probably contributed to enhancing antioxidant activities and color conversation in black chokeberry juice. A total of 40 volatiles were detected in the fermented black chokeberry juice by gas chromatography–ion mobility spectrometry. The off-flavor odors, such as 1-penten-3-one and propanal in the black chokeberry juice, were weakened after fermentation. The content of 2-pentanone significantly increased in all fermented juice, imparting an ethereal flavor. Hence, lactic acid fermentation can effectively enhance black chokeberry products' flavor and prebiotic value, offering valuable insights into their production. Practical Application The application of lactic acid bacteria in black chokeberry juice not only enhances its flavor but also improves its health benefits. This study has expanded the range of black chokeberry products and offers a new perspective for the development of the black chokeberry industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2024
·
IF 9.5
He Jinhua,Wang Chenhui,Liu Mingrui,Ramzan Muhammad,Long Zhiwei,Wu Xian-gang,Chen Yu,Zhong Haizheng
The potential use of large-size ZnSe quantum dots as blue emitters for display applications has greatly inspired the colloidal synthesis. Herein, we report the negative effects of side reactions of large-size ZnSe quantum dots. The side reactions between oleic acid and oleylamine generated amidation products and H 2 O, which led to the hydrolysis of Zn(OA) 2 to Zn(OH) 2 and the subsequent formation of zinc oxide (ZnO) and zinc bis[diphenylphosphinate] (Zn(DPPA) 2 ) precipitates. These side reactions resulted in the formation of a defective surface including a Se-rich surface and oxygen-related defects. Such negative effects can be overcome by adopting an etching strategy using potassium fluoride and myristic acid in combination. By overcoating a ZnS shell, blue emissive ZnSe/ZnS quantum dots with a maximum photoluminescence quantum yield of up to 91% were obtained. We further fabricated ZnSe quantum dots-based blue light-emitting diodes with an emission peak at 456 nm. The device showed a turn-on voltage of 2.7 V with a maximum external quantum efficiency of 4.2% and a maximum luminance of 1223 cd·m −2 .
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE NUTRITION
·
2024
·Vol. 2024
·期 1
·6670740
·
IF 3
Dingfei Ma,Qiangwei Li,Yuanyuan Xie,Youqin Kong,Zhili Ding,Jinyun Ye,Chenglong Wu,Yan Liu
Erucic acid (EA) in rapeseed oil has adverse effects on terrestrial animal and fish health. However, its antinutritional role in fish remains unclear due to the limited information on EA. Therefore, this study was conducted to assess the impact of EA on growth performance, antioxidative capacity, fatty acid profile, and lipid metabolism in tilapia. Six diets containing different amounts of EA (0, 3, 6, 12, 20, and 27 g/kg diet) were fed to tilapia (initial weight: 3.01 ± 0.01 g) for 8 weeks. The results exhibited that dietary EA did not affect growth performance but remarkedly increased the crude lipid contents (in the whole body, liver, and muscle). It also markedly increased the levels of low-density lipoprotein cholesterol, total cholesterol, nonesterified fatty acids, and triglyceride in the liver and serum in a dose-dependent manner. The EA groups had lower values of total superoxide dismutase, total antioxidant capacity, catalase, and higher activities of aspartate aminotransferase and alanine aminotransferase, as dietary EA levels increased. Feeding fish with diets containing EA (20 and 27 g/kg diet) significantly increased the malondialdehyde content. Moreover, dietary EA greatly altered the fatty acid profile in the liver and muscle. It especially elevated the percentages of C18 : 2n-6, C20 : 1n-9, and C22 : 1n-9 while decreasing the C18 : 0 and C16 : 0 levels. When the levels of EA in diets were 12, 20, and 27 g/kg, genes correlated with lipophagy, lipolysis, and β -oxidation were significantly reduced. Meanwhile, genes concerned in triglyceride synthesis were largely increased in the liver and muscle. In summary, high-dose EA (20 g/kg diet) in the diets significantly induced fat accumulation, hepatic oxidative damage, and abnormal lipid metabolism in tilapia. The current findings expand our understanding on the antinutritional role of EA in lipid homeostasis and fish health.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Hongxu Zhou,Qing Jiang,Xin He,Xian Fu,Jun-Yan Liu
Fatty acids (FAs) are essential molecules in all organisms and are involved in various physiological and pathophysiological processes. Pentafluorobenzyl bromide (PFBBr) is commonly used for FAs derivatization for gas chromatography-mass spectrometry (GC–MS) quantification by chemical ionization (CI). While CI is the conventional ionization mode for PFBBr derivatization, electron ionization (EI) source has also demonstrated efficacy in achieving satisfactory analytical performance for the analysis of PFB esters. In this study, we present a novel approach utilizing PFBBr-derivatization on a GC-EI-MS platform to quantitatively analyze a comprehensive range of 44 fatty acids (FAs) spanning from C2 to C24. The method's sensitivity, precision, accuracy, linearity, recovery, and matrix effect were rigorously validated against predetermined acceptance criteria. In comparison to the conventional CI ionization mode, the utilization of PFBBr-derivatization in GC-EI-MS exhibits a wider range of applications and achieves comparable sensitivity levels as the conventional CI platform. By using this method, we successfully quantified the 44 FAs in plasma and feces samples from the mice with deoxynivalenol (DON)-induced kidney injury. Among these, the levels of most FA species were increased in the DON-exposure group compared with the control group. The orthogonal partial least squares discriminant analysis (OPLS-DA) of all the tested FAs showed a visual separation of the two groups, indicating DON exposure resulted in a disturbance of FA profile in mice. These results indicate that the established method by integration of GC-MS with PFBBr derivatization is an efficient approach to quantify the comprehensive FA profile, which includes short-, medium- and long-chain FAs. In addition, our study provides new insights into the mechanism underlying DON exposure-induced kidney injury.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Yanzhi Dong,Jiaojiao Wang,Ting Zhou,Jinbing Pan,Xu Wang,Shao-Kai Sun
Highly sensitive iron oxide nanoparticles with stable, safe and efficient surface functionalization, as potential substitutes for gadolinium-based contrast agents (GBCAs) with increasing biosafety concerns, exhibit great potential for high-performance magnetic resonance angiography (MRA). Herein, we developed ultrasmall catechol-PEG-anchored ferrite nanoparticles (PEG-UMFNPs) for highly sensitive MRA. The obtained nanoprobe has a high T1 relaxivity value (7.2 mM−1 s−1) due to its ultrasmall size and Mn doping. It has a suitable hydrodynamic size of 20 nm, which prevents rapid vascular extravasation and renal clearance and prolongs its blood circulation time. In vivo MRA at 3.0 T using the nanoprobe shows that the arteries and veins of rats, even blood vessels as small as 0.32 mm, are distinctly visible, and the contrast enhancement can last for at least 1 h. In addition, due to the outstanding contrast enhancement and long circulation time, the stenosis and recanalization process of the rat’s carotid artery can be continuously monitored with a single injection of the nanoprobe. Our study indicates that PEG-UMFNPs are outstanding MR imaging nanoprobes that can be used to diagnose vascular diseases without the biosafety issues of GBCAs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Chuncao Xia,Huan Zhang,Mengmeng Xie,Jia-Ying Che,Quanqing Feng,Yihan Zhang,Guohang Ma,Minrui Liu,Sixian Hu,Yuan He,Xiaoli Liu,Zhenlin Li,Haiming Fan
Ferrite nanoparticles, known for their enzyme-like catalytic activity, have gained significant attention as innovative nanozymes for various catalysis medicine applications. However, the relationship between catalytic activity and ultrasmall ferrite nanoparticle composition remains unclear, which hinders the development of ferrite-based nanozymes with high catalytic performance. Here, we have synthesized a series of ultrasmall ferrite nanozymes for studying the composition dependent peroxidase (POD)-like activity. Initially, the size and surface charge were regulated to assess their impact on POD-like activity. The results indicate that smaller ferrite nanozymes with a negative charge exhibited superior activity when using TMB as the substrate. Subsequently, we examined the ultrasmall ferrite nanozymes with the same size and surface charge but different compositions (CoFe2O4, MnFe2O4, and γ-Fe2O3), and comprehensively investigated the effect of composition on POD-like activity. The results show that the POD-like activity is closely related to the composition of the ultrasmall ferrite nanozymes and the activity order towards TMB is found to be CoFe2O4 > MnFe2O4 > γ-Fe2O3. By comparing the catalytic performance of nanoparticles with different compositions, the influence of composition on their activity are elucidated. Furthermore, we determined that the optimal pH and temperature for the POD-like catalytic activity of ultrasmall CoFe2O4 nanozyme were pH=4-4.5 and 30 oC. Under these optimal catalytic conditions, the ultrasmall CoFe2O4 nanozymes exhibited a higher POD-like activity, resulting in increased tumor cell staining intensity. This suggests that ultrasmall CoFe2O4 nanozymes may serve as a viable alternative to horseradish peroxidase for immunohistochemical staining applications. This work provides experimental evidence for designing efficient ultrasmall ferrite catalysts for nanozyme catalysis medicine applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Huan Du,Fengrong Lv,Renye Yue,Yuzhen Yu,Xinyu Xu,Zhe Li,Baode Chen,Cheng Zhang,Yu Peng,Wei Cheng,Sulai Liu,Xiaobing Zhang,Guosheng Song
Adenosine triphosphate (ATP) is a central mediator of cancer cell metabolism and signaling, driving processes such as proliferation, invasion, metastasis, and therapeutic resistance. Current ATP imaging modalities-including fluorescence, chemiluminescence/bioluminescence, photoacoustic imaging, and magnetic resonance imaging (MRI)-face inherent trade-offs among sensitivity, spatial resolution, and tissue penetration capability. Here, a dual-mode probe is reported that synergistically couples the anatomical resolution of MRI with the high-sensitive, long-lasting signals of afterglow imaging for visualization of ATP in the tumor microenvironment. The probe is constructed via DNA hybridization between an afterglow unit and a T 1 -weighted MRI contrast agent, with an ATP-specific aptamer mediating probe disassembly upon ATP binding. This design generates simultaneous turn-on afterglow and MRI signals, enabling the imaging of intracellular and extracellular ATP fluctuations. Metabolic modulators-coenzyme Q10 (an ATP activator) and 2-deoxy-D-glucose (2-DG, a glycolysis inhibitor)-are applied to demonstrate dynamic, bidirectional tracking of ATP levels in vivo. Furthermore, by monitoring ATP depletion during synergistic metabolic therapy (cisplatin + 2-DG), a correlation between imaging readouts and therapeutic efficacy is established. The work provides anintegrated MRI/afterglow imaging platform for high-contrast, real-time ATP sensing in cancer, offering a powerful tool for elucidating tumor metabolism and evaluating metabolic interventions.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）