INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Huilian Yu,Saif Ali,Ke Yan,Wenqing Zhao,Shanshan Wang,Zhiguo Zhou,Wei Hu
Drought stress negatively affects cotton pollen fertility, which in turn leads to a decrease in seed number per boll and boll weight. Exogenous melatonin application significantly enhances pollen fertility under drought conditions, while the specific underlying mechanisms remain unclear. A pot experiment was conducted using a cultivar Yuzaomian 9110 under two moisture treatments (soil relative water content at 75 ± 5 % and 45 ± 5 %) with two melatonin concentration (0 and 200 μM) to investigate the effects of exogenous melatonin on the structural traits and physiological metabolism of cotton anthers and its’ relationships with pollen fertility. Results demonstrated the significant impact of drought on anthers development and metabolism, with damage to the anther tapetum and decreased starch and adenosine triphosphate (ATP) contents, subsequently resulting in reduced pollen germination rate, seed number per boll and boll weight. Melatonin application in water-deficit anthers up-regulated the expression of sucrose transporter protein ( GhSWEET55 ) and phosphate sucrose synthetase, promoting sucrose import and synthesis, respectively. However, it also increased sucrose synthase and acid convertase, accelerating sucrose decomposition and reducing its content. Additionally, melatonin application promoted starch accumulation in water-deficit anthers by enhancing activities of adenosine diphosphate glucose pyrophosphorylase and soluble starch synthase, meaning that potential energy storage was increased, which facilitated the formation of pollen fertility. Although melatonin application reduced the expression of pyruvate kinase ( GhPK ) and glucose 6-phosphogluconate dehydrogenase ( GhG6PD ) genes in water-deficit anthers, it up-regulated hexokinase ( GhHXK ) and citrate synthase ( GhCIT ) expression, enhancing ATP content, and ultimately pollen fertility, seed number and boll weight under drought. In summary, exogenous melatonin preserved cotton pollen fertility under drought stress by regulating carbohydrate and energy metabolism, especially enhancing starch and ATP accumulation in anthers.
研究领域:
植物生理学
·
农业科学
✓ 已验证
DOI
高影响因子
Plant Reproduction
·
2021
·Vol. 34
·期 2
·91-101
·
IF 3.767
Jia Xin-Lei,Xue Jing-Shi,Zhang Fang,Yao Chi,Shen Shi-Yi,Sui Chang-Xu,Peng Yu-Jia,Xu Qin-Lin,Feng Yi-Feng,Hu Wen-Jing,Xu Ping,Yang Zhong-Nan
The pollen coat, which forms on the pollen surface, consists of a lipid–protein matrix. It protects pollen from desiccation and is involved in adhesion, pollen–stigma recognition, and pollen hydration during interactions with the stigma. The classical methods used for pollen coat observation are scanning and transmission electron microscopy. In this work, we screened a collection of fluorescence dyes and identified two fluorescent brighteners FB-52 and FB-184. When they were used together with the exine-specific dye, Basic fuchsin, the pollen coat and the exine structures could be clearly visualized in the pollen of Brassica napus . This co-staining method was applied successfully in staining pollen from Fraxinus chinensis , Calystegia hederacea , and Petunia hybrida . Using this method, small pollen coat-containing cavities were detected in the outer pollen wall layer of Oryza sativa and Zea mays . We further showed these dyes are compatible with fluorescent protein markers. In the Arabidopsis thaliana transgenic line of GFP-tagged pollen coat protein GRP19, GRP19-GFP was observed to form particles at the periphery of pollen coat . This simple staining method is expected to be widely used for the studies of the palynology as well as the pollen–stigma interaction.
研究领域:
植物科学
·
孢粉学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2020
·Vol. 8
·期 7
·3335-3345
·
IF 1.797
Qi Chen,Gang Xue,Qinxue Ni,Yan Wang,Qianxin Gao,Youzuo Zhang,Guangzhi Xu
Gardenia ( Gardenia jasminoides J. Ellis) is regarded as an edible medicine plant in China. Here, gardenia flower polysaccharide fraction (GFPF) was extracted by water at 90°C and its chemical composition, rheological properties, and antioxidant activities of GFPF were investigated. The GFPF extraction yield was 18.04 ± 1.81% (W/W) and mainly comprised neutral sugars (46.83 ± 3.14%), uronic acid (35.21 ± 0.17%), protein (1.63 ± 0.34%), and total phenol (9.49 ± 0.08 mgGAE/g). Galacturonic acid (41.05 ± 0.59%) was the main monosaccharide, and galactose, glucose, arabinose, rhamnose, xylose, mannose, and glucuronic acid were also detected in GFPF. Its degree of esterification was 32.76 ± 1.52%. FT-IR spectra analysis showed a similar absorption pattern between GFPF and pectin from apple. The results suggested that GFPF was low methoxy pectin. Thermogravimetric analysis and zeta potential analysis indicated that the pectin was stable under high temperature and alkaline condition. Steady rheology showed that the GFPF dispersion was a shear thinned pseudoplastic fluid with high apparent viscosities at concentration above 2%. The degree of pseudoplasticity of the solutions increased with the concentrations increased and the temperatures decreased. DPPH and ABTS free radical scavenging assay indicated that GFPF had relatively high antioxidant activity. The results showed that gardenia flower was rich in pectin polysaccharides with low methoxy pectin. It had high apparent viscosities at concentration above 2% and had good antioxidant activity. The data suggested that GFPF can be a new resource of low methoxy pectin with potential application as thicker or gelling agents in food industry.
研究领域:
食品科学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Jianxia Sun,Haixia Luo,Xinghua Li,Xusheng Li,Yeyu Lu,Weibin Bai
Pyranoanthocyanins are anthocyanin derivatives that are formed during fermentation and oxygenation processes in aged wine. They play a critical role in assessing the aging of wine based on its colour quality. Naturally, the formation process of pyranoanthocyanins is fairly slow. In this respect, the present work studies the role and efficiency of ultrasonic treatment in accelerating the formation of methylpyranocyanidin-3- O -glucoside and investigates the spectral characteristics as well as the stability of this derivative toward heat, bisulfite bleaching and pH variation in a simulation system. Our results showed that the yield of methylpyranocyanidin-3- O -glucoside can be increased by 32.5% by ultrasonic treatment at 100 W for 40 min, and excessive treatment (≥60 min) does not have a positive effect on the yield. Moreover, this derivative exhibits a better stability toward heat, pH variation and bisulfite bleaching than its precursor cyanidin-3- O -glucoside.
研究领域:
食品科学
·
化学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Yu-fang Fu,Jian Zhu,Xin Li,Guo-jun Weng,Jian-jun Li,Jun-wu Zhao
In this work, we synthesized Au-Ag alloy nano-garlands with multiple petal-like branches and internal cavities in two steps. First, we etched silver nanospheres with HAuCl 4 to obtain Au-Ag alloy nanoshells with different shell layer thicknesses and hollow core sizes. After that, based on different nanoshells, 4-NTP was used as a shape-directing agent, HAuCl 4 and AgNO 3 as precursor raw materials, and AA as a reducing agent for the growth of petals to form Au-Ag bimetallic nano-garlands with multi-branching structure. The regulation of the branching morphology of the nano-garlands could be achieved by changing the amounts of 4-NTP and HAuCl 4 . The SERS activity of the nano-garlands could be further improved by changing the AA concentration, the size of nanoshells, and the Au/Ag ratio of the precursor raw materials. Because the bimetallic nano-garlands have hollow cavities and petal-like branches, there are couplings between the cavities and the branches, between the adjacent branches, and between the metals inside and outside of the cores, which can achieve a good enhancement of SERS. The highest SERS enhancement factor of Au-Ag bimetallic nano-garlands can reach 4.47×10 8 , which can be used as a good candidate for SERS substrate for the trace detection of biomolecules , pesticides, and other substances.
研究领域:
材料科学
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Rui Chen,Ying-Ying Wang,Xia Jiang,Ming-Hao Ji,Ke-Xian Li,Hao-Yan Shi,Hai-Long Wang,Yan-Xin Chen,Can-Zhong Lu
The wide band gap and low conductivity of TiO 2 limit its application for photocatalytic H 2 production. In this work, a nitrogen (N) and phosphorus (P) self-doped TiO 2 /Carbon (TiO 2 /C) composite with an egg yolk-shell structure was successfully synthesized using a lotus pollen template synthesis approach. The microstructure and photoelectrochemical performance of the composite materials were investigated. The introduction of hetero elements decreased the band gap of the TiO 2 /C composite, extending light absorption to the visible light region. Additionally, the enhanced conductivity facilitated fast charge transfer and prolonged the lifespan of electron/hole pairs. Consequently, the N, P self-doped TiO 2 /C composite exhibited higher H 2 production (656.18 μmol·g −1 ·h −1 ) than commercial TiO 2 (418.12 μmol·g −1 ·h −1 ) under simulated sunlight irradiation.
研究领域:
材料科学
·
能源与环境科学
✓ 已验证
产品关联
DOI
高影响因子
Chemical Engineering and Processing-Process Intensification
·
2020
·
IF 3.731
Sajjad Rasam,Mostafa Keshavarz Moraveji,Antonio Soria-Verdugo,Ali Salimi
An environmentally friendly adsorbent for the removal of lead (II) ions from aqueous solutions was prepared by hydrothermal carbonized of KOH activated Crocus sativus petals, generated as an agricultural residue. The influence of the operating parameters in the hydrothermal carbonization process on Pb 2+ adsorption capacity was evaluated by an artificial neural network method. The pore structure, morphology, functional groups and elemental composition of the activated hydrochar were characterized. The reaction temperature and residence time were found to be the main parameter affecting the physicochemical properties of the produced hydrochar. The optimal conditions of hydrothermal carbonization towards lead ions adsorption were as follows: temperature of 180 °C, residence time of 11.5 h, water/biomass ratio of 10 and ZnCl 2 /biomass ratio of 1.5, resulting in an adsorption capacity of 89.52 mg g −1 . The optimal hydrochar was selected for further investigation, including the kinetics and isotherms of adsorption.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qi Zhou,Lihong Wang,Canping Pan,Congcong Yu,Jin Hua,Tao Lan
Considering the matrix characteristics of honeysuckle and chrysanthemum, a comprehensive and systematic extraction and purification processes study for pesticide residue analysis in these two Traditional Chinese Medicine (TCM) were conducted based on a nanomaterial-modified QuEChERS method. For honeysuckle, a combination of 60 mg FeN-MWCNTs and 60 mg PSA provided the best purification effect. For chrysanthemum, 70 mg GCB and 30 mg PSA achieved the optimal results. Our initial exploration into the purification mechanism of the nanomaterials revealed that micropores and smaller mesopores in the adsorbents play a crucial role, while amino groups primarily govern the adsorption process, with matrix impurities adhering to the surface of the nano-adsorbents. The limit of quantification (LOQ) for honeysuckle in this method ranged from 0.12 to 18.86 µg/kg, with recovery rates between 60.1 % and 128.6 %, and relative standard deviations (RSD) less than 12 %. For chrysanthemum, the LOQ was between 0.10 and 8.56 µg/kg, and the recovery rates for 27 pesticides were in the range of 61.6 % to 118.9 %. Applying this method, 105 honeysuckle and 111 chrysanthemum market samples were analyzed, detecting five pesticides in 18 honeysuckle and 12 chrysanthemum samples respectively. This study offers a targeted strategy for the development of analytical methods for trace substances in complex matrices.
研究领域:
分析化学
·
中药学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Yanlong Shao,Jie Zhao,Yong Fan,Zhenping Wan,Longsheng Lu,Zhihui Zhang,Weihua Ming,Luquan Ren
Superhydrophobic surface with tunable water adhesion has attracted great interests due to its unique performances in manipulating the status of water stay and roll-off. Herein, inspired by two typical superhydrophobic models of rose petal and lotus leaves, a superhydrophobic surface that can reversibly transform between the Cassie-Baxter state and the Cassie impregnating state has been developed by regulating the shape memory polymer SMP micro/nanostructures. The superhydrophobic surface with intact micro/nanostructure arrays exhibits excellent self-cleaning properties with extraordinary low water adhesive force, whereas the superhydrophobic surface with compressed microstructure arrays demonstrates high water adhesion. In response to the heating triggered deforming-restoring action, the switchable superwetting transition between those two models can be easily realized by their transformed morphologies. Due to the good shape memory effect of the polymer, the structured SMP assay surfaces can display a multiply switchable transition between the “lotus-effect” and the “rose-petal effect”, revealing a great potential for rewritable liquid patterns, controlled droplet transportation.
研究领域:
材料科学
·
表面工程学
✓ 已验证
DOI
高影响因子
Insects
·
2024
·Vol. 15
·期 11
·866
·
IF 2.7
Chen Wang,Dianxuan Wang,Fangfang Zeng,Liang Chen,Xinxin Zhao,Xi Zhu,Junji Yao,Yihan Li
Simple SummaryThe Indian meal moth,Plodia interpunctella(Hübner, 1813) (Lepidoptera: Pyralidae), exhibited significant differences in oviposition preference between normal-oleic peanuts (NOPs) and high-oleic peanuts (HOPs). In this study, we found key volatile organic compounds (VOCs) from NOP and HOP peanuts that were attractive or repellent toP. interpunctellafemales by gas chromatography/mass spectrometry (GC-MS), gas chromatography/electroantennographic detection (GC-EAD), and electroantennogram (EAG) analysis, as well as behavioral responses in Y-tube olfactometer and wind tunnel bioassays. The identification of key VOCs may provide a foundation for developing food-based attractants and repellents for pest control.The Indian meal moth,Plodia interpunctella(Hübner, 1813) (Lepidoptera: Pyralidae), a primary stored peanut insect pest, exhibited a significant difference in oviposition preference among normal-oleic peanuts (NOPs) and high-oleic peanuts (HOPs). Identifying key volatile organic compounds (VOCs) that are attractive or repellent toP. interpunctellais of great significance for the ecological management of pests. The profiles and contents of VOCs among NOP and HOP varieties were measured and compared, and key bioactive VOCs were further confirmed via an electroantennogram (EAG) analysis, as well as behavioral responses in Y-tube olfactometer and wind tunnel bioassays. Females prefer to lay eggs on NOP varieties more than on HOP ones. Acetophenone, nonanal, decanal, dodecane, 2,5-dimethylbenzaldehyde, and 4-ethyl-benzaldehy derived from tested peanuts elicited stronger antennal EAG responses. The results of the Y-tube olfactometer and wind tunnel bioassay showed that the relative high levels of nonanal, dodecane, and unique VOC acetophenone in NOP varieties have a significant attraction toP. interpunctella. Conversely, 4-ethyl-benzaldehyde and the unique VOC 2,5-dimethyl-benzaldehyde commonly found in HOP varieties exhibit notable repellent effects onP. interpunctella. These VOCs could contribute to the development of attractants or repellents derived from special peanut varieties for pest management.
研究领域:
昆虫学
·
生态学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2015
·
IF 2.818
Qian Li,Jun Chen,Ti Li,Chengmei Liu,Xieyi Wang,Taotao Dai,David Julian McClements,Jiyan Liu
The influence of passage through a simulated gastrointestinal tract (GIT) on the stability and bioaccessibility of proanthocyanidins isolated from fruit ( Choerospondias axillaris ) peel was studied. In addition, the effects of the simulated GIT on the antioxidant and α-glucosidase inhibitory activities of proanthocyanidins extracts were evaluated. Gastric digestion had little effect on total polyphenol content (TPC) or mean degree of polymerization (mDP) of crude and purified extracts. However, intestinal digestion led to a significant decrease (about 26% and 19%) in TPC and (about 12% and 7%) in mDP for crude and purified extracts, respectively. The observed reduction in TPC and mDP levels was attributed to interactions of proanthocyanidins with pancreatic enzymes, rather than due to the chemical conditions during digestion. Only small flavan-3-ol molecules (monomers, dimers and trimers) could diffuse into the dialysis tubing used to simulate the intestinal wall. Changes in antioxidant activity during digestion were correlated to changes in TPC. After simulated GIT digestion, over 85% of α-glucosidase inhibitory activity of both extracts was preserved. These results indicate that the majority of the proanthocyanidins maintained their biological activities after passage through the simulated GIT, and were therefore still capable of providing valuable health benefits.
研究领域:
食品科学
·
营养学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2014
·Vol. 22
·期 6
·4654-4659
·
IF 2.757
Yao Lixian,Huang Lianxi,He Zhaohuan,Zhou Changming,Li Guoliang,Deng Xiancai
Roxarsone (ROX), a world widely used feed organoarsenic additive in animal production, can be excreted as itself and its metabolites in animal manure. Animal manure is commonly land applied with phosphorous (P) fertilizer to enhance the P phytoavailability in agriculture. We investigated the accumulation of As species in garland chrysanthemum ( C. coronarium ) plants fertilized with 1 % ( w / w , manure/soil) chicken manure bearing ROX and its metabolites, plus 0, 0.05, 0.1, 0.2, 0.4, and 0.8 g P 2 O 5 /kg, respectively. The results show that As(III) was the sole As compound in garland chrysanthemum shoots, and As(III) and As(V) were detectable in roots. Elevated phosphate level supplied more As(V) for garland chrysanthemum roots through competitive desorption in rhizosphere, leading to significantly enhanced accumulation of As species in plants. As(III) was the predominant As form in plants (85.0∼90.6 %). Phosphate could not change the allocation of As species in plants. Hence, the traditional practice that animal manure is applied with P fertilizer may inadvertently increase the potential risk of As contamination in crop via the way ROX → animal → animal manure → soil → crop.
研究领域:
植物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）