Oxidative Medicine and Cellular Longevity
·
2021
·
IF 6.543
Wei-Bo Huang,Guo-Jun Zou,Gu-Hua Tang,Xin-Yuan Sun,Jian-Ming Ouyang
The original Laminaria polysaccharide (LP0) was sulfated using the sulfur trioxide-pyridine method, and four sulfated Laminaria polysaccharides (SLPs) were obtained, namely, SLP1, SLP2, SLP3, and SLP4. The sulfated (–OSO3–) contents were 8.58%, 15.1%, 22.8%, and 31.3%, respectively. The structures of the polysaccharides were characterized using a Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance (NMR) techniques. SLPs showed better antioxidant activity than LP0, increased the concentration of soluble Ca2+ in the solution, reduced the amount of CaOx precipitation and degree of CaOx crystal aggregation, induced COD crystal formation, and protected HK-2 cells from damage caused by nanometer calcium oxalate crystals. These effects can inhibit the formation of CaOx kidney stones. The biological activity of the polysaccharides increased with the content of –OSO3−, that is, the biological activities of the polysaccharides had the following order: LP0 < SLP1 < SLP2 < SLP3 < SLP4. These results reveal that SLPs with high –OSO3− contents are potential drugs for effectively inhibiting the formation of CaOx stones.
研究领域:
药理学
·
材料科学
·
细胞生物学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 10
·3524-3546
·
IF 6.6
Jing-Hong Liu,Jian-Ming Ouyang
Objective: The first objective is to study the synergistic inhibition of calcium oxalate (CaOx) formation by Laminarin polysaccharides (DLP and SDLP, before and after sulfation) and potassium citrate (K3cit) and determine the synergistic protection of renal epithelial cells (HK-2 cells) caused by CaOx crystal damage. The second objective is to explore new ways to prevent and treat kidney stones. Methods: The CaOx crystals regulated by five additives (K3cit group, DLP group, SDLP group, DLP–K3cit synergistic group and SDLP–K3cit synergistic group) were characterized by FT-IR, XRD, SEM, zeta potential, ICP, and TGA. The protective effect of each additive group on HK-2 cells damaged by nano-calcium oxalate monohydrate (nano-COM) was compared by detecting cell viability, the cell reactive oxygen species level, the cell survival rate, and mitochondrial membrane potential. Results: When DLP or SDLP acted synergically with K3cit, the synergistic group induced the same amount of COD at a lower concentration or more COD formation at the same concentration, highlighting the synergistic enhancement effect of 1 + 1 > 2. At 0.3 g L−1, the COD contents induced by DLP, SDLP, K3cit, DLP–K3cit, and SDLP–K3cit synergistic groups were 20.3%, 75.8%, 75.4%, 87.3%, and 100%, respectively. The synergistic group increased the concentration of soluble Ca2+ ions in the supernatant, increased the absolute value of the zeta potential on the surface of CaOx crystals, and inhibited the aggregation among the crystals. TGA and DTG analyses established the adsorption of polysaccharides in the crystals. Cell experiments showed the ability of the synergistic group to significantly inhibit the damage of nano-COM crystals on HK-2 cells, reduce the level of reactive oxygen species and mortality, and improve cell viability and the mitochondrial membrane potential. Conclusions: The synergistic group can more effectively induce COD formation and cell protection than the standalone polysaccharide group or K3cit group. The synergistic groups, especially SDLP–K3cit, may be a potential drug for inhibiting the formation of CaOx kidney stones.
研究领域:
医学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Yue Shen,Jingyuan Zhou,Bin Wu,Mengni Ge,Naixin Wang,Yan Zhao,Quan-Fu An,Bart Van der Bruggen
Membrane scaling is one of the drawbacks of nanofiltration (NF) impeding its wide application in treatment of salt-containing water. Especially, the high concentration of Ca 2+ and SO 4 2− easily lead to a CaSO 4 scale layer on the membrane surface, limiting the separation process. Herein, a zwitterionic NF membrane with anti-scaling performance was fabricated through multi-carboxyl carboxylate (sodium citrate) incorporated by interfacial polymerization. Due to the synergistic effect of sodium citrate, the resulting NF membrane had a more negatively charged surface and a larger pore size than the pristine membrane. In desalination, this zwitterionic NF membrane exhibited a high selectivity (15.9) between SO 4 2− (73.1 ± 2.4% rejection) and Ca 2+ (4.6 ± 1.4% rejection) with a flux of 39.6 LMH, much higher than that of the commercial NF membrane (NF-270, a selectivity of 1.5). Based on the high SO 4 2− and Ca 2+ selectivity, it is difficult to form a CaSO 4 scale layer on the membrane surface, which yields an excellent anti-scaling performance of the resulting membrane. Furthermore, the zwitterionic monomer endowed the membrane with an anti-fouling effect due to zwitterions materials have strong hydration to resist nonspecific protein adsorption. This design strategy of ion selectivity zwitterionic membranes through the incorporation of carboxylate provides guidance for the design of highly ion selective anti-scaling NF membranes, which has potential applications in water treatment for salt-containing solutions.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2024
·
IF 2
Wentao Chen,Yue Gui,Huayu Li,Xiran Shen,Xiaoli Liu,Fan Liu,Hong Chen
The solubility of calcium oxalate monohydrate (COM) has been measured in aqueous solutions of various naturally sourced organic small molecules such as carboxylic acids, amino acids, and polyphenols. All of these compounds enhanced its solubility, with carboxylic acids such as citric and malic acid exerting the most pronounced impact, within the addition concentration range of up to 100 μM. The observed solubilities were well correlated at all concentrations using a theoretical model that incorporates the specific interaction theory (SIT) specifically developed for this system. The complexation of these molecules with calcium ions was found to play a major role, which is subject to the material and charge balance. The induction time was also measured and found to increase with all of the compounds studied, suggesting their capability to bind with calcium.
研究领域:
生物化学
·
医学
✓ 已验证
DOI
高影响因子
Small
·
2025
·
IF 12.1
Quan Zhang,Bang-Xian Yu,Jun Long,Xue-Wu Chen,Fang Huang,Wen-Qi Wu,Yong-Da Liu,Guo-Hua Zeng,Ling-Hong Huang,Xin-Yuan Sun
Kidney stones, which have high prevalence and recurrence rates, often cause severe oxidative damage and inflammation. The ultrasmall hydrodynamic diameter of nanoparticles is crucial for their enrichment in the kidneys to exert biological activity. However, integrating crystallization inhibition and therapeutic functions into a single ultrasmall nanoparticle is challenging. A novel ultrasmall iron (Fe)–gallic acid (Ga) metal–phenolic networks (Fe–Ga MPNs) is developed for treating calcium oxalate (CaOx) nephropathies. These MPNs can specifically adsorb on the high-energy ( 1 ¯ 01 $\bar{1}01$ ) crystal face to inhibit the growth of CaOx monohydrate (COM), promoting the phase transition from highly toxic COM to low-risk CaOx dihydrate. Fe–Ga MPNs have broad-spectrum free radical scavenging abilities, reducing oxidative damage and inhibiting cell apoptosis. They exhibit sensitivity toward kidney damage, accumulating in injured renal tissue, reducing tubule injury and inflammation, improving tubule function, and inhibiting crystal formation. Fe–Ga MPNs also inhibit pro-inflammatory macrophage polarization and upregulate anti-inflammatory and highly phagocytic macrophage polarization. RNA sequencing analysis shows that Fe–Ga MPNs induce transcriptomic changes mainly involving immune regulation and citrate homeostasis pathways. In conclusion, the multifunctional nanonetwork Fe–Ga MPNs, with crystallization inhibition, antioxidant, and immune regulation properties, show great potential in treating CaOx nephropathies.
研究领域:
医学
·
材料科学
·
肾脏病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-24
·
IF 12.6
Tong Xin-Yi,Nong Wei-Jian,Tan Run-Min,Ouyang Jian-Ming
Objective This study aimed to compare the differences in pyroptosis and polarization of macrophages induced by different sizes of calcium oxalate monohydrate (COM) crystals and discuss the mechanism of kidney stone formation. Methods COM crystals with sizes of about 100 nm, 500 nm, 2.5 μm, and 10 μm were synthesized and characterized. THP-1 cells were induced to differentiate into macrophage-like cells. The damage of COM crystals to Mφs was evaluated by CCK-8 assay. The activation of the pyroptosis signaling pathway, cell morphology, and proportion of pyroptosis cells in Mφs induced by COM crystals were detected. Finally, the proportion of M1/M2 polarization was qualitatively and quantitatively assessed. Results COM-100 nm, COM-500 nm, COM-2.5 μm, and COM-10 μm all significantly induced the pyroptosis and polarization of Mφs. Among them, at the same cell viability, COM-10 μm induced the highest pyroptosis rate. The expression of NLRP3 and GSDMD-N induced by COM-10 μm increased by 81.43 ± 11.45% and 112.00 ± 9.87%, respectively, and the rate of caspase-1/PI double-positive pyroptosis cells was 22.2%. Meanwhile, COM-10 μm induced the highest proportion of M1 polarization (M1/M2 ratio was 209.90% ± 2.54%). Conclusion COM-10 μm significantly activates the classical caspase-1-mediated pyroptosis pathway and promotes M1 polarization in Mφs. Therefore, at the same cell viability, large-sized micro-COM is more capable of inducing inflammation than nano-COM. Graphical
研究领域:
纳米技术
·
泌尿科学
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免疫学
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细胞生物学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yu He,Ejun Peng,Xiaozhuo Ba,Jian Wu,Wen Deng,Qiu Huang,Yonghua Tong,Haojie Shang,Zichen Zhong,Xiao Liu,Yanlong Zhang,Tao Ye,Xiaoqi Yang,Kangyang Wang,Yabin Xie,Kehua Jiang,Ding Xia,Zhiqiang Chen,Kun Tang
Emerging studies have demonstrated that M1 macrophage polarization and oxidative stress play important roles in calcium oxalate (CaOx) induced kidney injury, which leads to increased crystals deposition. ROS scavenging nanozymes and kidney-targeted nanoparticles for antioxidant drugs delivery have emerged as an arisen methodology for kidney injury therapy. However, cell membrane biomimetic-modified nanozymes as anti-inflammatory drug delivery systems for the treatment of kidney injury is rarely reported. Herein, the ROS responsive red blood cell-membrane-coated resatorvid-loaded cerium oxide nanoparticles (RBCM@CeO 2 /TAK-242) are constructed to suppress CaOx induced kidney injury and crystals deposition. In vitro, RBCM@CeO 2 /TAK-242 shows effective internalization by renal tubular epithelial cells, along with demonstrated antioxidative, anti-inflammatory, and macrophage reprogramming effects. Glyoxalate(Gly)-induced renal CaOx crystals mouse model is established, RBCM@CeO 2 /TAK-242 shows excellent injured kidney targeting and biosafety, and could effectively suppress CaOx induced kidney injury and crystals deposition. RBCM@CeO 2 /TAK-242 has a dual protective effect by both inhibiting oxidative stress and modulating macrophage polarization in vivo. In addition, RNA seq analysis reveals that RBCM@CeO 2 /TAK-242 protects against CaOx induced kidney injury via suppressing the TLR4/NF-κB pathway. This study provides an innovative strategy for RBCM@CeO 2 /TAK-242 as injured kidney targeting and dual protective effects for the treatment of CaOx induced kidney injury and crystals deposition.
研究领域:
纳米技术
·
药理学
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肾脏生理学
·
炎症
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氧化应激
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Han Li,Yushan Li,Huailing Diao,Haipeng Ren,Jianming Chen,Jinfeng Wang,Qingtao Liu,Xungai Wang
Wild silk cocoons are light-weight composite structures with unique properties. One such property is the intriguing CO 2 gating property (i.e., unidirectional flow of CO 2 ) exhibited by the wild Antheraea pernyi ( A. pernyi ) silkworm cocoon. This study investigated the hierarchical structural and compositional features of A. pernyi cocoons and their impact on CO 2 adsorption. For the first time, soap-film flowmeter testing method and gas permeability testing method were used to quantitatively demonstrate the A. pernyi cocoons’ CO 2 adsorption property and selectivity for gas mixture. The results showed that the multi-layered composite structure of A. pernyi cocoons, coupled with calcium oxalate crystals, play a crucial role in their CO 2 adsorption and separation performance. These findings shed light on the unique functional design of wild silk cocoons and provide a new biomimetic approach for developing materials and structures to address global challenges such as CO 2 capture and storage.
研究领域:
材料科学
·
环境科学
·
仿生学
·
气体分离
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2021
·
IF 4.219
Lei Yu,Xiuguo Gan,Yufeng Bai,Ruihua An
Kidney stone disease (KSD) is a common urinary disease with increasing prevalence worldwide. In this study, we investigated the effect of cyclic AMP responsive element binding protein (CREB) 1 in a KSD model of rat and calcium oxalate monohydrate (COM) crystals-treated NRK-52E cells. Rats were pretreated with lentivirus (LV)-CREB1 vector or LV-control vector and administrated with ethylene glycol + ammonium chloride to induce KSD. It was found that CREB1 was activated in the renal tissue of non-treated KSD rats. Pretreating with LV-CREB1 vector significantly enhanced CREB1 expression in KSD rats. Biochemical analysis for serum and urine showed that upregulation of CREB1 could improve the renal function of KSD rats. Histological analysis confirmed that upregulation of CREB1 alleviated the renal injury in KSD rats. Moreover, the upregulation of CREB1 suppressed the apoptosis in renal tissue of KSD rats through regulating apoptosis-associated proteins. Further study showed that the upregulation of CREB1 could attenuate the oxidative stress in KSD rats as well. More interestingly, the upregulation of CREB1 enhanced the activity of complex I and complex III and the expression of mitochondrial cytochrome c , implicating the effect of CREB1 on improving mitochondrial function in KSD rats. In vitro study confirmed that upregulation of CREB1 inhibited the apoptosis and oxidative stress, while improved the mitochondrial function of NRK-52E cells treated with COM crystals, demonstrating the protective effect of CREB1 on COM crystals-induced renal epithelial cell injury. Therefore, CREB1 might be served as a promising target in the prophylaxis and treatment of KSD.
研究领域:
肾病学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCES IN POLYMER TECHNOLOGY
·
2018
·Vol. 37
·期 8
·2859-2865
·
IF 2.073
Qiuying Li,Tenghui Chen,Lewen Sun,Weihong Guo,Chifei Wu
This work aimed at providing a suitable method for preparing the cross-linked ultra-high-molecular-weight polyethylene (UHMWPE). First, UHMWPE was grafted with a silane compound using organic peroxide as an initiator in the molten state. Then, cross-linking was performed by compression molding of the mixture of the silane-grafted UHMWPE and CaC 2 O 4 ·H 2 O. Water released from CaC 2 O 4 ·H 2 O made the silane-grafted UHMWPE cross-link. In particular, no catalyst was added in the cross-linking step. Fourier Transform Infrared results confirmed that the silane has been grafted onto the UHMWPE chain successfully and the grafting index varied with the dosage of the initiator. With the increasing of CaC 2 O 4 ·H 2 O content, the gel ratio increased and average molecular weight of the cross-linked UHMWPE decreased, which proved that the cross-linked UHMWPE can be prepared by this method. Moreover, there existed different cross-linked network structures (loose and tight networks) in the samples with the same gel ratio.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Yuan Su,Si Li,Xin Li,Jing-Ying Zhou,Vraj P. Chauhan,Meng Li,Ya-Hui Su,Chun-Mei Liu,Yi-Fei Ren,Wu Yin,Jeffrey D. Rimer,Ting Cai
Kidney stones are a pervasive disease with notoriously high recurrence rates that require more effective treatment strategies. Herein, tartronic acid is introduced as an efficient inhibitor of calcium oxalate monohydrate (COM) crystallization, which is the most prevalent constituent of human kidney stones. A combination of in situ experimental techniques and simulations are employed to compare the inhibitory effects of tartronic acid with those of its molecular analogs. Tartronic acid exhibits an affinity for binding to rapidly growing apical surfaces of COM crystals, thus setting it apart from other inhibitors such as citric acid, the current preventative treatment for kidney stones. Bulk crystallization and in situ atomic force microscopy (AFM) measurements confirm the mechanism by which tartronic acid interacts with COM crystal surfaces and inhibits growth. These findings are consistent with in vivo studies that reveal the efficacy of tartronic acid is similar to that of citric acid in mouse models of hyperoxaluria regarding their inhibitory effect on stone formation and alleviating stone-related physical harm. In summary, these findings highlight the potential of tartronic acid as a promising alternative to citric acid for the management of calcium oxalate nephropathies, offering a new option for clinical intervention in cases of kidney stones.
研究领域:
泌尿学
·
材料科学
·
生物物理学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Sun Xin-Yuan,Gan Qiong-Zhi,Ouyang Jian-Ming
Urinary crystals with various sizes are present in healthy individuals and patients with kidney stone; however, the cellular uptake mechanism of calcium oxalate of various sizes has not been elucidated. This study aims to compare the internalization of nano-/micron-sized (50 nm, 100 nm, and 1 μm) calcium oxalate monohydrate (COM) and dihydrate (COD) crystals in African green monkey renal epithelial (Vero) cells. The internalization and adhesion of COM and COD crystals to Vero cells were enhanced with decreasing crystal size. Cell death rate was positively related to the amount of adhered and internalized crystals and exhibited higher correlation with internalization than that with adhesion. Vero cells mainly internalized nano-sized COM and COD crystals through clathrin-mediated pathways as well as micron-sized crystals through macropinocytosis. The internalized COM and COD crystals were distributed in the lysosomes and destroyed lysosomal integrity to some extent. The results of this study indicated that the size of crystal affected cellular uptake mechanism, and may provide an enlightenment for finding potential inhibitors of crystal uptake, thereby decreasing cell injury and the occurrence of kidney stones.
研究领域:
细胞生物学
·
肾病学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）