ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Zhichao Liu,Shengnan Wang,Wanying Li,Yang Tian
Iron ions, as a main component of intracellular labile iron, not only play an important function in oxygen transport, enzymatic reactions, and electron transport but also are vitally important in oxidative stress. In this work, we developed a ratiometric fluorescent biosensor for ferrous ion (Fe2+), in which gold nanoclusters (AuNCs) were synthesized as a stable fluorescent probe and a ligand (FeL) was designed for specific recognition of Fe2+ and conjugated onto AuNCs (AuNC@FeL). Meanwhile, water-soluble sulfocyanine 7 N-hydroxysuccinimide ester (Cy7 NHS ester) was immobilized onto AuNC@FeL as a reference element. The developed ratiometric fluorescent nansosensor displayed good linearity with the concentration of Fe2+ in the range of 1–105 μM, and detection limit was achieved down to 210 nM. In addition, this nanosensor responded to Fe2+ in less than 1.23 s and showed high selectivity against other metal ions, amino acids, and reactive oxygen species. With the advantages of high selectivity and accuracy, as well as quick response and long-term stability, this organic–inorganic ratiometric fluorescent probe was successfully applied in real-time biosensing and bioimaging of Fe2+ in neurons and HepG2 cells. By use of this tool, it was found that the increasing concentration of Fe2+ in live cells was closely related to oxidative stress.
研究领域:
生物成像
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细胞生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Dan Qiao,Zhen Zhang,Lingshuang Wang,Wei Sheng,Qiliang Deng,Shuo Wang
Test strips as one of micro-devices are widely concerned in the fields of point-of-care detection and in-field diagnosing. For the purpose of on-site detection of tyramine, a carbon dots-molecularly imprinted fluorescent sensing test strip with high selectivity has been successfully obtained via a rapid in-situ preparation procedure (about 2.5 h). Here, the layer composed of carbon dots-molecularly imprinted polymer (CDs-MIP) with the function of the recognition and signal transduce has been formed on the surface of the filter paper strips via the sol-gel method. The developed test strip displayed a high specificity (Imprinting factor, IF = 4.96); and its fluorescence emission was quenched linearly with the increase of tyramine concentration from 0.5 to 10.0 mg L −1 . The limit of detection (LOD) is 0.059 mg L −1 (3 σ/s ). The application potential was verified by sensing of tyramine in rice vinegar and white rice vinegar with the recoveries of 90.9-104.4% (RSD < 5.8%). Many advantages including a rapid preparation and high selectivity make the fluorescence strip suitable for on-site detection of tyramine. In addition, the preparation procedure can also be easily transferred to the preparation of the fluorescence strip toward other targets.
研究领域:
分析化学
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食品科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Jun-Dan An,Tian-Tian Wang,Yang-Fan Shi,Jian-Zhong Huo,Xiang-Xia Wu,Yuan-Yuan Liu,Bin Ding
In recent years, a new type of micro-porous material, namely metal organic framework material, has received more and more attention from many basic and industrial fields because these materials possess unique advantages. In this work, through the powerful sonochemical preparation method, a three-dimensional cluster-based Cd II -MOFs, {[Cd(abtz) 2 (H 2 O) 2 ]·(ClO 4 ) 2 ·H 2 O} n ( 1 , abtz = 1-(4-aminobenzyl)-1H-1,2,4-triazole) can be quickly synthesized in the facile ultrasonic method. Powder X-ray diffraction (PXRD) measurement confirms that these bulky samples 1 (synthesized on different ultrasonic powers and ultrasonic time conditions) were pure . In addition, ultrasonic chemical time and irradiation power did not change the structure of composites materials 1 . SEM and morphological changes of 1 in the ultrasonic synthesis are also determined. Moreover, 1 exhibits good stability, the structure of 1 can be maintained not just in various solvents, and in aqueous environments with pH values from 2 to 12. Photo-luminescent experiment also reveals that complex 1 has the excellent application prospect as highly sensitive sensing material for the biomarker DPA (2,6-pyridine dicarboxylic acid) and 5-HT (5-hydroxytryptamine) through the photo-luminescence “turn-on” and “turn-off” effect, respectively. Further photo-luminescent measurements also show that different ultrasonic powers and ultrasonic time can effectively induce fluorescent sensing enhancement for biomarkers DPA and 5-HT based on the water stable clustered-based cadmium(II) coordination framework.
研究领域:
材料科学
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生物学
·
化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-12
·
IF 5.3
Zhou Xiayu,Wang Yue,Song Jiayi,Xiong Lihao,Zhao Xin,Chen Sihan,Zhao Weichao,Li Le,Zhen Deshuai
Three types of carbon dots were synthesized using the same precursor (folic acid and europium nitrate) via different preparation methods (doping and direct coordination). A comprehensive comparison and analysis of the morphology, surface groups, and optical properties of the prepared carbon dots (CD), europium-doped carbon dots (CD-Eu), and europium-functionalized carbon dots (CD@Eu) were conducted. Moreover, due to the higher quantum yield, excellent stability, and outstanding selectivity for UO 2 2+ exhibited by CD-Eu, we selected CD-Eu as the probe for subsequent applications. CD-Eu showed a sensitive response to UO 2 2+ within the concentration range 25 ~ 200 nM, with a detection limit of 0.84 nM (0.42 μg·L −1 ). Additionally, CD-Eu demonstrated excellent accuracy and recovery in spiked detection of real water samples. Furthermore, we discovered that this probe could detect UO 2 2+ both in vitro and in viv o. This strategy provides a promising fluorescent sensor for the detection of UO 2 2+ in water and biological samples, holding significant implications for addressing UO 2 2+ contamination issues. Graphical abstract
研究领域:
材料科学
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2025
·Vol. 15
·期 1
·420-427
·
IF 4.6
Zongmei Huang,Jing Li,Lu-Shuang Li
Levodopa (L-Dopa), a precursor drug for dopamine has been widely used to treat Parkinson's disease. However, excess accumulation of L-Dopa in the body may cause movement disorders and uncontrollable emotions. Therefore, it is vital to monitor L-Dopa levels in patients. In this study, a carbon dot (CD)-based fluorescence sensing system was developed for sensitive detection of L-Dopa. The CDs were prepared using a novel biomass, Pandanus amaryllifolius Roxb., as a carbon source via a simple hydrothermal method. Interestingly, it was found that ethylenediamine doping in the preparation system increased the quantum yield of CDs, as well as their fluorescence response sensitivity to L-Dopa. After optimizing the preparation and sensing conditions, the detection limit of L-Dopa decreased from 1.54 μM to 0.05 μM. A complete methodological validation was conducted and the probe was successfully applied to the determination of L-Dopa in fetal bovine serum with excellent precision (RSD ≤ 2.99%) and recoveries of 88.50–99.71%. Overall, this work provides an effective strategy for the regulation of properties of CDs derived from biomass and an innovative method for clinical L-Dopa monitoring.
研究领域:
医学诊断
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材料科学
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药物分析
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 12
·2805-2813
·
IF 3.637
Li Wang,Zhang Xiaoyue,Miao Chunyue,Li Ruijun,Ji Yibing
Paper-based devices have been very much in the foreground of analytical science recently. This work innovatively proposed a fluorescent paper–based sensor (FPS) constructed on a hybrid polydimethylsiloxane (PDMS)/paper platform where cellulose papers functionalized with carbon dots (CDs) as fluorophores by Schiff base chemistry were loaded on the grooves array of a designed PDMS plate. As a proof of concept, the performance of FPS was investigated with folic acid (FA) as the target analyte. Under optimal conditions, FPS enabled a rapid fluorescence quenching response to FA via inner filter effect in a wide range of 1–300 μmol L −1 with the limit of detection of 0.28 μmol L −1 . The feasibility of FPS was further verified by the detection of FA in orange juice and urine samples with satisfactory results. The covalent modification of CDs on paper endowed the FPS with good assay reproducibility and stability. Interestingly, FPS achieved a more sensitive assay of FA than the conventional strategy, by which the same CDs were directly used to detect FA in a solution-based system. The FPS illuminated a novel strategy for construction of reliable and sensitive assays based on paper-based devices. It is of paramount importance for its practical application in biosensing and clinical diagnosis.
Graphical abstract
研究领域:
分析化学
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生物传感
·
临床诊断
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DOI
高影响因子
LUMINESCENCE
·
2020
·Vol. 35
·期 6
·897-902
·
IF 1.855
Keke Ning,Guoqiang Xiang,Cuicui Wang,Feihong Huang,Jinzhi Liu,Lanlan Zhang,Minmin Yan,Bingqian Hu,Wen Lei
A ‘turn-on’ fluorescence method for detection of hydrogen peroxide (H 2 O 2 ) in marine food samples is presented in this article. Using this method, a carbon dots (CDs)–MnO 2 probe was formed in which fluorescence intensity (FI) of CDs was quenched through fluorescence resonance energy transfer by addition of MnO 2 nanosheets. When H 2 O 2 was added into the CDs–MnO 2 solution, the MnO 2 nanosheets formed Mn 2+ ions due to a redox reaction between H 2 O 2 and MnO 2 nanosheets, and CD FI was recovered. Under optimized conditions, the detection limit for H 2 O 2 was 0.87 μM, and analytical linear range was 4–100 μM. Furthermore, this developed fluorescence sensing system was successfully used with satisfactory results to determine trace H 2 O 2 content in marine food samples.
研究领域:
分析化学
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食品科学
·
海洋生物学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Zengchen Liu,Weijie Yang,Wenping Zhu,Hongyu Chen,Yahong Chen,Ruilan Liu,Yali Cui,Yanxia Li,Baodui Wang
Fluorine is one of the essential trace elements for human body, which is essential for calcification process in living organisms. In the paper, a “off–on” fluorescence sensor for fluorine ion based on aluminium ion and boric acid codoped carbon dots was synthesized and characterized. According to fluorescence analysis, the water-soluble sensor exhibited highly selective and sensitive for fluorine ion. In sensing process, F - could bind to Al 3+ after the addition of fluorine ion, which resulted in the decomposition of Al-BA-CDs system, then intense yellow fluorescence signal appeared. Moreover, the fluorescence sensor exhibited low detecting limit (5.0 nanomolar level) by fluorescence titration spectrum. In addition, according to evaluate the fluorescence sensing properties of F - in HepG2 cell and zebra fish, this job greatly extended the use of F - sensor from aluminium ion and boric acid codoped carbon dots in organism. The work also provided an interesting idea for fabricating water-soluble fluorescence sensor for F - .
研究领域:
分析化学
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生物医学
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环境科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yan Shen,Tianjing Zhang,Haoran Zhao,Yu Xiao,Jie Hong,Mohamed Abbas,Wanliang Yang
Portable and reusable solid-state fluorescence sensors have received much attention in the field of metal ion detection as a superior alternative to traditional liquid-based fluorescence sensors. In this study, a novel HHSS@LaVO 4 :Eu 3+ /polyacrylamide/sodium alginate (HLE/PS) hydrogel was successfully constructed for portable dynamic fluorescence switching detection of Fe 3+ and encryption of security information. The HLE/PS hydrogel was synthesized from HHSS@LaVO 4 :Eu 3+ fluorescent nanofillers embedded in a semi-IPN network of polyacrylamide (PAM) and sodium alginate (SA). The HLE/PS hydrogel has demonstrated excellent performance in the detection of Fe 3+ in aqueous solutions with high selectivity, high sensitivity, fast response (8 min), wide detection range (2–1000 μM), low detection limit (0.78 μM) and ease of use. It can be reused up to five times, underlining its utility and economy as a good performance fluorescence sensor. Notably, the quenching of Fe 3+ fluorescence by ultrasmall LaVO 4 :Eu 3+ nanoparticles on HHSS supports was significantly enhanced compared to conventional large-sized rare earth phosphor fillers. Additionally, by exploiting the reversible fluorescence switching function of the HLE/PS hydrogel, we further developed a high-security multi-layer information encryption platform. Finally, we thoroughly investigated the fluorescence response mechanism of HLE/PS hydrogels to Fe 3+ ions. This work not only provides a representative case for the preparation of packed rare-earth fluorescent hydrogels, but also demonstrates their potential as multifunctional smart luminescent materials.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·
IF 3.3
Shaoying He,Wei Lin,Xin Liu,Fei Li,Hong Liang,Huo Xu,Chunhua Lu,Chao Xing
Lead pollution presents a significant threat to ecological systems and human health, underscoring the urgent need for highly sensitive detection methods. Herein, we introduce a novel DNA concatemer-encoded CRISPR/Cas12a fluorescence sensor (MDD-Cas12a) for sensitive detection of Pb2+ based on DNAzymes. To accomplish this, we designed a substrate strand containing a long DNA concatemer encoding multiple protospacer adjacent motifs (PAMs) and protospacer sequences for activation of the CRISPR/Cas12a system. The DNA concatemer was subsequently anchored to the surface of magnetic beads (MBs) to fabricate a MBs-DNA concatemer nanoprobe. In the presence of Pb2+, the DNAzyme structure catalyzes the cleavage of the substrate strand, leading to the release of DNA concatemers. Following magnetic separation, the released DNA concatemers significantly activate the non-specific trans-cleavage activity of the Cas12a/crRNA complex. The fluorescence reporter DNA is then completely cleaved by the activated Cas12a/crRNA complex, and the Pb2+ concentration in the sample can be quantified by measuring the fluorescence signal. By harnessing the specific recognition capability of DNAzymes for Pb2+, the programmability of DNA concatemers, and the self-amplification features of the CRISPR/Cas12a system, the MDD-Cas12a platform demonstrates high sensitivity and specificity for detecting Pb2+ in milk and lake water samples.
研究领域:
生物化学
·
分子生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Minghui Zan,Shuai An,Lei Cao,Yulu Liu,Li Li,Mingfeng Ge,Pai Liu,Zaihui Wu,Wen-Fei Dong,Qian Mei
Progesterone (P4) is an important steroid hormone that act as an indicator in the female reproductive processes; thus, following its concentration levels in human serum is important for diagnosis of early pregnancy. In this work, carbon dots (CDs) were designed via a dry-heating method, and the novel CDs exhibited good dispersion stability and excellent photostability. Under optimal conditions, the as-prepared CDs displayed high selectivity and sensitivity for P4 and exhibited a fast-responsive (within 1 min) fluorescence intensity enhancement signal in aqueous solution. For further application, the fluorescence sensing system was further extended to monitoring progesterone in real samples with acceptable recoveries. The methodology developed here offers a promising direction for the detection of clinical biomarkers that are closely related to human health.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Yaqian Feng,Ruxue Li,Peng Zhou,Chunying Duan
A novel fluorescence carbon dots (CS-CDs) were prepared via a one-step hydrothermal approach using chitosan and urea as precursors. CS-CDs exhibited good selectivity and sensitivity of fluorescence response to Cr (VI) in a wide range with a detection limit of 0.59 μM. More interestingly, CS-CDs-based fluorescent hydrogel sensors were further developed for quantitative detection of Cr (VI) in water. The fluorescence signals of the composite were recorded by a smartphone and converted to grayscale parameters linearly related to concentration of Cr (VI) by Image J software. This composite showed a broad linear range of 10–300 μM and a low detection limit of 2.83 μM. All results demonstrate the great potential for the portable, low-cost and green detection of Cr (VI) in practical application.
研究领域:
环境科学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）