FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Qingqing Yu,Tong Shi,Zhiyu Xiong,Li Yuan,Hui Hong,Ruichang Gao,Yulong Bao
In order to study the effect of oxidation on the dye-binding behavior of myofibrillar proteins, selected dyes with different charges (positively charged Sarfarin O (SO), neutral bromophenol blue (BPB), and negatively charged Orange G (OG)) were incubated with myofibrils oxidized by the Fenton system with H 2 O 2 (10 mM). Upon oxidation, loss of free thiols, formation of carbonyls, particle size, and hydrophobicity of myofibrillar proteins (MPs) increased. The absolute value of Zeta-potential increased by 14.48 % after oxidation, the myofibrillar proteins shifted to a more acidic isoelectric point (pI) upon oxidation. Oxidation decreased net positive charges of myofibrillar protein and the binding ability of MPs towards OG in the environment with pH less than pI and the affinity of MPs towards SO in the environment with pH more than pI were thus increased. Here we propose a hypothesis that oxidation-induced change in net charges is the driving force affecting the amount of protein-bound dye. This paper aims to examine the effect of oxidation on the net charges of myofibrillar proteins and to provide insight into the mechanism of oxidation-induced changes in protein-bound dyes.
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FOOD CHEMISTRY
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2022
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IF 9.231
Yuechun Li,Han Zhang,Zhaowen Cui,Sijie Liu,Jingke Xu,Conghui Jia,Yaqian Chen,Lulu Wang,Jing Sun,Daohong Zhang,Mingqiang Zhu,Jianlong Wang
Exploring a novel strategy for strengthening the catalytic activity of enzyme facilitates the development of a sensitive enzyme-linked immunosorbent assay (ELISA). Herein, a chemical staining (CS) strategy was firstly discovered to possess the ability to directly improve the catalytic activity of horseradish peroxidase. Based on this discovery, coomassie brilliant blue was introduced into ELISA to establish a CS enhanced ELISA (CS-ELISA) to detect clenbuterol (CL) by simply staining monoclonal antibodies. Satisfactorily, the most important analytical parameters of CS-ELISA, including sensitivity (0.074 ng mL −1 ) and linear range (0.2–2 ng mL −1 ) were all improving 2-folds compared with conventional ELISA. Moreover, the CS-ELISA shows good applicability in the detection of CL in pork tenderloin samples. The proposed CS-ELISA shows various advantages, such as cost-effective, easily accessible, enhanced catalytic activity of enzyme, higher sensitivity, and broader linear range, providing a new insight into enhanced ELISA for food safety.
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INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Jiahui Wei,Hao Ren,Huamin Zhai,Shengcheng Zhai
Green, efficient depolymerization of lignocellulose is a prerequisite and the key in high-value utilization of its components. Understanding of the changes in morphological chemistry of the cell wall, related to the biomass recalcitrance, is critical for optimizing the processing conditions of cell wall deconstruction. Thus, herein, based on our previous studies, subcritical water autohydrolysis of naked oat stems was conducted at 190 °C and 1.18 MPa for 0–60 min. The depolymerization behaviors of tissues and cell walls were monitored dynamically at 10 min intervals. The micro-and ultrastructural changes of autohydrolyzed residue sections were analyzed. Parenchyma tissue commenced dissociation after 10 min at 190 ℃, whereas mechanical tissue dissociation occurred after 40 min at 190 ℃, along with interlayer separation of the secondary walls of sclerenchyma fibers. The autofluorescence changes of lignin showed that the order of regional lignin removal in tissues was from sieve tubes and companion cells to parenchyma cells, and finally to sclerenchyma fibers and vessel elements. The turning point of tissue and cell wall structural depolymerization of naked oat stems under subcritical water autohydrolysis conditions occurred after 40 min at 190 ℃ and 1.18 MPa. Under this condition, the degree of tissue dissociation was as high as 80%, while the fiber cell morphology remained intact. Dynamic changes in the tissue and cell wall structures and cell morphologies of naked oat stems maintained at 190 ℃ for various durations were captured by differentiating the use of the optical microscope, fluorescence microscope , and scanning electron microscope . The extensive, detailed assay data yield novel insights into the dynamic depolymerization mechanism of lignocellulosic composite cell walls under subcritical water autohydrolysis conditions.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
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2022
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IF 10.319
Yuanting Xu,Wanting Lin,Dandan Yuan,Shifan Chen,Fang Li,Yanping Long,Chao He,Weifeng Zhao,Changsheng Zhao
Adsorption and photocatalysis are regarded as two desirable technologies for wastewater remediation, but are still unsatisfactory in removal effect, eco-friendly regeneration and facile reusability. In this study, we developed a composite nanofibrous membrane material with excellent removal performance for organic pollutants based on synergistic adsorption and photocatalysis. A novel boron-doped, nitrogen-deficient graphitic carbon nitride (B-C 3 N 4 ) photocatalyst as well as an amphiphilic copolymer of methyl methacrylate and acrylic acid (p(MMA-AA)) were synthesized respectively, and then used to modify polyethersulfone for the fabrication of composite nanofibrous membrane with improved hydrophilicity, negatively-charge property and enhanced visible light response simultaneously. Subsequently, the synergistic effect of adsorption and photocatalytic degradation for organic pollutants were identified especially and resulted in an excellent removal efficiency even superior to the combination of adsorption and photocatalytic degradation, which could be called a “1+1>2” effect. In addition, the regeneration and reusability, the purification ability for multicomponent wastewater, and the photocatalytic mechanism, were investigated and discussed systematically. In this work, we not only prepared the nanofibrous membrane with synergistic effect of adsorption and photocatalysis, but also provided a versatile approach to design dual-functional support material to ensure the practical applications of powdery photocatalyst in wastewater treatment.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
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IF 4.539
Chongling Wang,Na Li,Pengfei Bian,Guohua Li,Jingyue Yang,Qing Li,Tifeng Jiao
Molecular self-assembly is a technology in which different structural components spontaneously form assemblies with special functions through weak, reversible non-covalent interactions on the basis of molecular recognition . The amphiphilic and biocompatibility of polypeptides make it has natural advantage in self-assembly at the air-water interface. In this work, the self-assembled films of palmitoyl pentapeptide-4 (PP4) and dye molecules were prepared by Langmuir-Blodgett (LB) technology. It was found that the PP4/dye composite film had good acid-alkali gas response. In addition, the LB film prepared by PP4 and methyl orange (MO) showed chiral signal, which was reflected in the circular dichroism spectrum. The current research work provides new clues for the preparation of functional self-assembled films and their applications as gas sensors and chemical switches.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2023
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IF 5.2
Lei Jin,An-Ni Zheng,Mei Wang,Jia-Qiang Yang,Zhao-Yun Wang,Fang-Zu Yang,De-Yin Wu,Dongping Zhan
In this work, we develop a novel safranine (SA)-based acidic sulfate copper electronic plating process for through-hole copper void-free filling for high-quality electronics interconnection, and both the molecule actions and synergistic effects of safranine (SA) are carefully explored. Cyclic voltammetry experiments at rotating rates of 200 r/min and 1200 r/min demonstrate that SA itself can accelerate the electro-reduction of copper ion , heighten the inhibiting effect of polyethylene glycol (PEG) and acceleration effect of bis-(3-sulfopropyl) disulfide (SPS). The addition of SA to the virgin make-up solution (VMS) containing PEG and SPS makes the relative electric charge (∆ Q ) between the low and high rotating rates be increased from 1.27 ± 0.04–22.53% ± 0.05, indicating the higher copper electronic plating amount at the center than that at the mouth of through-hole. In situ Fourier transform infrared spectroscopy together with theoretical calculations further illustrate that the molecule SA is adsorbed on the copper surface through the N atom in the aromatic ring , PEG and SPS can heighten the adsorption of SA on the Cu surface, and both PEG and SPS both synergistically influence with SA to achieve the void-free copper filling of through-hole.
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JOURNAL OF MEMBRANE SCIENCE
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2021
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IF 8.742
Fangmeng Sheng,Xingya Li,Yuanyuan Li,Noor Ul Afsar,Zhang Zhao,Liang Ge,Tongwen Xu
Wastewater containing organic dyes and high content of salts has posed a serious challenge on global water security. Herein, a cationic nanoporous covalent organic framework (COF) membrane is constructed via a facile interfacial growth strategy. The prepared membranes show excellent dye/salt separation performance with a pure water flux above 24 L m −2 h −1 bar −1 . The rejection rate of methylene blue of positive charges is above 99%, while the rejection rates of NaCl, Na 2 SO 4 , MgSO 4 are only 3.2%, 2.6%, and 9.4%, respectively. The high rejection of methylene blue is mainly governed by the electrostatic repulsion between cationic COF channels and the positively charged dye. Moreover, the size sieving effect dominates the rejection of dyes with larger molecular sizes than that of the COF channel. These findings provide strategies for developing various nanoporous COF membranes for efficient dye/salt separation.
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Advanced Healthcare Materials
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2023
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IF 10
Lei Lei,Rui Cong,Yifei Ni,Xin Cui,Xulei Wang,Hongmei Ren,Zun Wang,Mengyuan Liu,Jiasheng Tu,Lei Jiang
Osteoarthritis (OA) is a prevalent, chronic degenerative disease that affects people worldwide. It is characterized by the destruction of cartilage and inflammatory reactions. High levels of reactive oxygen species (ROS) cause oxidative stress, which damages lipids, proteins, and DNA, leading to cell damage and death. Furthermore, ROS also induces the production of inflammatory cytokines and cell chemotaxis, further worsening the inflammatory response and damaging cartilage resulted in limited movement. Herein, this work reports a dual-functional injectable hydrogel, which can help inhibit inflammation by scavenging ROS and provide lubrication to reduce wear and tear on the joints. To create the hydrogel, 3-aminophenylboronic acid modified hyaluronic acid is synthesized, then which is crosslinked with hydroxyl-containing polyvinyl alcohol (PVA) to construct a dual dynamic covalent crosslinked hydrogel oHA-PBA-PVA gel, Gel (HPP). The hydrogel mentioned here possesses a unique bond structure that allows it to be injected, self-heal, and provide lubrication. This innovative approach offers a new possibility for treating osteoarthritis by combining anti-inflammatory and lubrication effects.
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TALANTA
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2023
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IF 6.1
Kai Chen,Huiru Dong,Ziyi Ni,Yan Zhao,Yinyin Qian,Yuzhi Wang,Kaijia Xu
In this work, eight kinds of hydrophobic deep eutectic solvents (DESs), including four types of ionic and four types of non-ionic DESs, were prepared and applied in vortex-assisted liquid-liquid microextraction (LLME) technology. To explore the extraction ability of the hydrophobic DESs-based vortex-assisted LLME, four types of dyes were selected as analytes, involving anionic tartrazine (TA), amaranth (AM) and cationic phenosafranine (PF), methylene blue (MB). It turned out that the ionic and non-ionic hydrophobic DESs showed selective extraction on anionic and cationic dyes, respectively. In particular, the extraction efficiency of TA could reach 99.3 % when trioctylmethylammonium chloride-thymol ([TMAC][Thy]) was utilized as extraction agent. The partitioning efficiency of PF was up to 99.9 % by using decanoic acid-thymol ([DecA][Thy]) as extraction agent. The limits of detection (LODs) of TA and PF were 0.06 and 0.14 μg mL −1 , respectively. The limits of quantification (LOQs) obtained for TA and PF were 0.20 and 0.47 μg mL −1 , respectively. Besides, FT-IR and 1 H NMR were utilized to investigate the extraction mechanism. The results demonstrated that the hydrogen bonding and electrostatic force were the main driving forces in the extraction process. Furthermore, through separating various anionic and cationic dyes, the selective extraction ability of [TMAC][Thy] and [DecA][Thy] were successfully verified. Hence, the feasible operation, high extraction efficiency and excellent selectivity make the developed hydrophobic DESs-based vortex-assisted LLME attractive in dyes separation.
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Nano Today
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2021
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IF 20.722
Yang Liu,Xiaoping Zhang,Tengling Wu,Bo Liu,Jianhai Yang,Wenguang Liu
Injectable hydrogels and stem cells have been increasingly applied to treat myocardial infarction (MI). However, the harsh microenvironment adversely affects the survival of implanted stem cells and ultimate therapeutic outcomes. Herein, we design and prepare rat bone marrow mesenchymal stem cells (rBMSCs)-embedded polyzwitterionic microgels ( [email protected] ) via in situ Michael addition reaction between multivinyl carboxybetaine macromonomer and thiol-ended polyethylene glycol mixed with rBMSCs in a microfluidic system. The [email protected] are then loaded into a tyramine-modified hyaluronic acid (HA-Tyr), into which Chinese herb puerarin (PUE) and horseradish peroxidase (HRP)/H 2 O 2 are added to catalyze inter-phenol crosslinks to form an injectable HA-Tyr-PUE hydrogel encapsulating [email protected] ( [email protected] @microgels). This [email protected] @microgels is injected into the infarct region of rats, where HA-Tyr-PUE hydrogel serves to favorably modulate the microenvironment by scavenging active oxygen and releasing oxygen, and rBMSCs remain stemness in highly hydrophilic microgels and exert a paracrine effect to facilitate anti-inflammation and angiogenesis . These concerted actions boost the highly efficient restoration of ventricular function and structure. The ROS-scavenging medicine-crosslinked biodegradable biomacromolecule hydrogel bearing stemness-maintained microgels can be expanded to diverse tissue engineering and regenerative medicine fields.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Jiying Men,Hongxing Shi,Chengya Dong,Yuanyuan Yang,Yuanrui Han,Ruixin Wang,Yiqing Zhang,Ting Zhao,Jun Li
A novel adsorbent with high adsorption capacity to remove cationic dyes was synthesized. Sodium 4-styrene sulfonate (SSS) was grafted polymerization on the surface of magnetic chitosan microspheres via -NH 2 /S 2 O 8 2− surface initiating system, obtaining MCS-g-PSSS microspheres. The grafted microsphere was characterized by Fourier transforms infrared spectroscopy , X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, vibration sample magnetometer and the Brunauer-Emmett-Teller. Cationic dyes were adsorbed by MCS-g-PSSS and methylene blue(MB) was acted as a typical example. The adsorption performance was explored by varying experimental conditions. The results showed the maximal adsorption capacity was 989 mg/g at pH 1 at 25 °C. The pseudo-second order model was found to be applicable for the adsorption kinetics . The adsorption capacity increased with rising temperature and it decreased owing to adding of ions. The adsorption isotherms were the best fitted by Langmuir. MCS-g-PSSS for MB showed high adsorption capacity due to the strong electrostatic interactions and π-π stacking, which was explained by FTIR and XPS and was verified by DFT calculations. The degree of adsorption spontaneity increased with rising the temperature. The grafted MCS-g-PSSS microspheres had high adsorption capacity for various kinds of cationic dyes and excellent for remove MB in the aqueous solution.
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CHEMICAL ENGINEERING JOURNAL
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2021
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IF 13.273
Yang Liu,Rui Guo,Tengling Wu,Yuanning Lyu,Meng Xiao,Binbin He,Guanwei Fan,Jianhai Yang,Wenguang Liu
Conventional strategies for constructing an injectable conductive hydrogel for treatment of myocardial infarction (MI) are mostly involved with complicated bioactive modification and incorporation of external conductive materials or cells/therapeutic drugs, severely restraining its medical translation. Herein, a cell-free and therapeutic drug-free injectable zwitterionic hydrogel is fabricated by thiol-acrylate Michael addition reaction between acrylate modified polycarboxybetaine macromonomer and dithiothreitol (DTT), where the free DTT and the tethered-DTT endow the hydrogel with an antioxidant ability. The zwitterionic hydrogel is optimized to exhibit an appropriate gelation time and injectability. Intriguingly, the cation-anion ion pair on the side chain affords an intrinsic conductive property similar to the native myocardium. After injection into the infarcted region of rats, the hydrogel can downregulate level of inflammatory factors, and upregulate the expressions of cardiac-related factors, angiogenic factors, and transmission electrical signal-relevant Cx-43, accompanied by suppression of oxidant stress and cardiac fibrosis and increased vessel density, supposedly due to inherent antifouling, vascularization-promoting and conductive properties. Echocardiography and histological analyses demonstrate a remarkable improvement of the functions and structure of infarcted myocardium in rats in terms of 2.17/2.80-fold increase in ejection fraction/fractional shortening, 3.62-fold decline in end-systolic volume, and 2.39-fold decrease in fibrosis area, compared to MI group. This cell-free and therapeutic drug-free injectable zwitterionic hydrogel represents a new approach to seek a simplified but holistic biomaterial for treating myocardial infarction.
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