INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
GuoHua Wu,Peng Song,DongYang Zhang,ZeYu Liu,Long Li,HuiMing Huang,HongPing Zhao,NanNan Wang,YanQiu Zhu
This paper reports the impacts of direct feeding silkworms with different nanoparticles (Cu, Fe, and TiO 2 ) on the morphology, structures, and mechanical properties of the resulting silk fiber (SF). The contents of the Cu nanoparticles were 38 times higher in the posterior silk glands and only 2–3 times higher in the SF and in the middle silk glands compared with the controlled groups. Significant changes of the surface morphology, structures, and diameter of the Cu nanoparticle fed SF have been observed, which are attributed to a slight SF protein reconstruction or conformational change in the mixture of silk fibroin and sericin in the silk glands. The resulting Cu-containing SF exhibits good tensile strength of 360 MPa and reaches a strain of 38%, which are 89% and 36% higher than those of the natural SF. This study offers a new green strategy for the easy modification to achieve robust composite SF.
研究领域:
材料科学
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生物工程
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产品关联
DOI
PubMed
高影响因子
Journal of Proteomics
·
2019
·
IF 3.537
Ruru Chen,Cheng Zhu,Mingzhou Hu,Lian Zhou,Hui Yang,Hailing Zheng,Yang Zhou,Zhiwen Hu,Zhiqin Peng,Bing Wang
Achieving efficient identification of silk protein requires highly sensitive analytical techniques and favorable extraction methods , which is of great significance to the research of ancient silk, especially for the controversial issue of the silk origin. In this paper, proteomics and western blot were proposed to analyze the silk proteins of Bombyx mori ( B. mori ) and Antheraea pernyi ( A. pernyi ) dissolved by different methods. First, the differences in secondary structure were detected via spectroscopy . LC-MS/MS was then employed to characterize the peptides of silk proteins precisely. LiBr solution exhibited outstanding dissolution effect on B. mori cocoon, with 87 proteins detected; while copper-ethylenediamine solution (CED) was more appropriate for A. pernyi cocoon, and 16 proteins were identified in A. pernyi -CED. In addition to fibroin and sericin , abundant seroins, enzymes , protease inhibitors , other functional proteins and uncharacterized proteins were detected. Based on the LC-MS/MS data, diagnostic antibodies for the two species were prepared, and fibroin was successfully identified by western blot assay because both dissolution methods were gentle and did not destroy the antigenic epitopes in the protein molecule. Owing to their good specificity and high sensitivity, these diagnostic antibodies have good application prospects in immunoassays of different silk species. Significance This study presents the comprehensive analysis on silk identification of proteins from B. mori and A. pernyi extracted by different methods via the proteomic and immunology as well as the conventional approaches. Great coverage of two cocoon proteomes was accomplished, which demonstrated the outstanding difference in components and abundance. Based on the proteomics analysis, the diagnostic antibodies against two species were prepared and identified the corresponding fibroin successfully in the completed protein mixtures. To our knowledge, the proteomic and immunology procedures with high efficiency, sensitivity and specificity are novel analysis on the silk identification and has great potential in the field of ancient silk detection.
研究领域:
蛋白质组学
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分析化学
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免疫学
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考古科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·Vol. 99
·期 2
·e21499
·
IF 1.316
Lin Zhu,De-Qing Zou,Zuo-Qing Fan,Na Wang,Ying-Ying Bo,Yu-Qing Zhang,Guang Guo
In this study, a carboxymethyl chitosan derived from silkworm pupa (SP-carboxymethyl chitosan) was prepared. The physical characteristics of the SP chitin, chitosan, and carboxymethyl chitosan were analyzed. The scanning electron microscopy results showed that the surfaces of the samples from SP were more uneven, with more surface fractures compared with those of the reference substance (RS). Thermal analysis, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy analysis showed that the main molecular chain structures of SP samples and RSs had no substantial differences. However, the crystallinity and thermal decomposition temperature of the SP samples were lower compared with those of the RSs. All of these results provide a theoretical basis for the development of applications for the SP-carboxymethyl chitosan.
研究领域:
生物材料学
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化学工程
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Jia Ke,Yingnan Zhu,Jiamin Zhang,Jing Yang,Hongshuang Guo,Weiqiang Zhao,Chiyu Wen,Lei Zhang
Silk is an attractive natural fiber due to its excellent mechanical properties and biocompatibility. Xenobiotic modification of pristine silk is gaining significant attention to meet specific demands. The silk payload of xenobiotics is affected by their uptake efficiency, biodistribution, metabolism, and excretion. Herein, we systematically evaluated the effect of the nanoparticle size (20–100 nm) on the biodistribution and silk production by introducing silver nanoparticles (AgNPs) into the silkworm diet. Interestingly, the silk payload of AgNPs is highly dependent on their overall size, where 50 nm AgNPs are prone to accumulate in silk glands and reveal the highest content in the produced silk, qualifying silk antibacterial activity against both Gram-positive and Gram-negative bacteria. These findings provide new insights to understand how nanoparticle properties influence the incorporation of nanomaterials into silk and can help optimize the material design.
研究领域:
生物材料学
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纳米生物学
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昆虫学
✓ 已验证
DOI
高影响因子
MOLECULES
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2016
·Vol. 21
·期 4
·408
·
IF 2.465
Yahui Ge,Yuping Tang,Sheng Guo,Xin Liu,Zhenhua Zhu,Lili Zhang,Pei Liu,Shaoxiong Ding,Xiangzhi Lin,Rurong Lin,Jin-ao Duan
To evaluate the nutritional and functional value ofSipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases inS. nuduswithin 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches ofS. nudussamples from four habitats with two different processing methods. The results showed thatS. nuduscontained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts ofS. nuduswere significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control ofS. nudusand other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development ofS. nudus.Keywords:UPLC-TQ-MS/MS;Sipunculus nudus;free amino acid;nucleoside;nucleobase
研究领域:
海洋生物学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Journal of Natural Fibers
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2022
·
IF 3.507
Yun Wang,Xin Liu,Liangjun Xia,Chunhua Zhang,Weilin Xu
Silk fibers were produced by feeding Bombyx mori silkworm larvae with a modified feed of mulberry leaves containing nano Fe3O4 particles. The silkworms were weighed to determine the effect of nano Fe3O4 particles on the growth of larvae. The effects of nano Fe3O4 contents on the morphologies, chemical structures, mechanical properties, and thermal properties of silk were also investigated, respectively. The results indicate that the nano Fe3O4 particles was embedded in the silk. Additionally, the stress and toughness of modified silk were enhanced, as compared with the normal silk. Finally, the integration of the amide I band intensities suggests a relatively high content of β-sheet in the modified silk.
研究领域:
生物学
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材料科学
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产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 10
·4131
·
IF 4.6
Guohua Wu,Lei Jiang,Jianjun Guo,Wushuang Li,Lin Ma,Bozhi Tang,Charles C. Liu
Alanine transaminase (ALT) is an important amino acid-metabolizing enzyme in silkwormBombyx moriL., and is mainly involved in transferring glutamate to alanine (serving as an essential precursor in silk protein synthesis) through transamination. Therefore, it is generally believed that silk protein synthesis in the silk gland and the cocoon quantity increase with the increase in ALT activity to a certain extent. Here, a novel analytical method was developed to determine the ALT activity in several key tissues ofBombyx moriL. including the posterior silk gland, midgut, fat body, middle silk gland, trachea and hemolymph, by combining the direct-analysis-in-real-time (DART) ion source with a triple-quadrupole mass spectrometer. In addition, a traditional ALT activity assay, the Reitman–Frankel method, was also used to measure ALT activity for comparison. The ALT activity results obtained via the DART-MS method are in good agreement with those obtained via the Reitman–Frankel method. However, the present DART-MS method provides a more convenient, rapid and environmentally friendly quantitative method for ALT measurement. Especially, this method can also monitor ALT activity in different tissues ofBombyx moriL. in real time.Keywords:ALT;DART-MS;Bombyx mori;biochemical analysis
研究领域:
昆虫学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2024
·
IF 6.2
Xing Zhang,Yifei Kong,Zihan He,Wenbin Yu,Wenjing Shao,Chengliang Gong,Wenlin Zhou,Xiaolong Hu
Perfluorooctanoic acid (PFOA) and tetrabromobisphenol A (TBBP-A) are emerging environmental contaminants with recognized potential health and ecological risks. This study investigated the effects of PFOA and TBBP-A exposure on the global of metabolites of silkworm gut with GC-MS metabolomics. Our results revealed distinct metabolic alterations in silkworms exposed to PFOA and TBBP-A, highlighting their differential impacts on silkworm health and productivity. Exposure to these chemicals significantly altered metabolic profiles, leading to disruptions in pathways related to lipid, carbohydrate, and amino acid metabolism. These findings suggest that PFOA and TBBP-A disrupt crucial metabolic processes in silkworms, indicating potential toxicity and prompting further investigation into their effects on human health and the environment. Ongoing research is crucial to develop safer alternatives and mitigate the risks associated with these persistent contaminants.
研究领域:
环境科学
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昆虫学
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代谢生物学
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产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2025
·
IF 7.5
Xinyao Zhang,Ke Cheng,Lixue Dong,Dexing Zhu,Qingyong Li,Zhongbao Guo,Yongju Luo,Apeng Liu,Juan Tian,Di Peng,Jianmin Zhang,Yangyang Liu,Hua Wen,Ming Jiang
Fava bean is currently utilized as a specialized feed ingredient to enhance the muscle texture of fish. When incorporated at appropriate levels, fava beans can improve fish muscle crispy texture. However, high dietary levels of fava beans may lead to liver damage in fish, although the specific mechanisms behind this effect remain unclear. In this study, 600 genetic improvement of farmed tilapia (GIFT; Oreochromis niloticus ) of uniform size (initial body weight 552.3 ± 10.12 g) and healthy appearance were randomly divided into four groups, each with three replicates of 50 fishes. The fish diets contained different levels of fava beans (0, 50%, 60%, and 70%), designated as D0, D50, D60, and D70, respectively, over a ten-week feeding experiment. A comprehensive analysis of the impact of fava beans on muscle texture and liver health in GIFT was performed. The results showed that 50%, 60%, and 70% dietary fava beans had no significant effect on the growth performance of GIFT ( P > 0.05), but 70% dietary fava beans increased feed conversion ratio (FCR) ( P = 0.022). Consumption of fava beans at levels of 50%, 60%, and 70% in the diet altered GIFT muscle texture ( P < 0.05), and adversely effected serum biochemistry and liver health ( P < 0.05). Non-targeted metabolome analysis of the liver revealed that dietary fava beans may cause irreversible damage to liver cells by disrupting the DNA methylation process ( P < 0.05). This finding enhances the understanding of the mechanisms behind the negative impact of fava bean consumption on liver function. It is crucial to investigate potential strategies to mitigate these adverse effects while maintaining the desired muscle quality when fava beans are used in aquatic feed.
研究领域:
水产养殖学
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分子生物学
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营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ECONOMIC ENTOMOLOGY
·
2025
·
IF 2.4
Tian Chao,Tang Jie,Zhu Qingyu,Guo Xiqian,Shu Qilong,Gu Zhiya,Li Fanchi,Li Bing
Bombyx mori (L.) (Lepidoptera: Bombycidae) is an important economic insect, and Exorista sorbillans (W.) (Diptera: Tachinidae) is an endoparasitic pest of larval B. mori. Dimethoate is less toxic to B. mori than E. sorbillans and is used in sericulture to controlling E. sorbillans. To investigate the effects of dimethoate treatment on the gut microorganisms and physiological functions of B. mori, 16S rRNA sequencing was used to analyzed the composition and structure of the gut microbiota. This study investigated their role in enhancing silkworm resistance by screening dominant populations after dimethoate treatment. The results indicated that dimethoate did not alter the composition of the dominant gut bacterial groups in silkworm; however, it significantly increased the abundance of the gut bacteria Methylobacterium and Aureimonas, and decreased the abundance of Enterobacterales, Bifidobacterium, Blautia, Collinsella, Faecalibacterium, and Prevotella. Eleven strains of dimethoate-resistant bacteria were selected through in vitro culture, all of which were unable to grow when dimethoate was used as a carbon source. Additionally, a germ-free silkworm model was established to assess detoxifying enzyme activity in the midgut. The results revealed that the gut symbiotic microbiota can enhance dimethoate resistance by increasing detoxification enzyme activity. This study identifies a novel pathway for silkworm resistance to dimethoate based on gut microbiota, providing new insights into the role of symbiotic gut bacteria in insecticide metabolism.
研究领域:
昆虫学
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微生物学
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农药科学
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产品关联
DOI
PubMed
高影响因子
Materials
·
2018
·Vol. 12
·期 1
·14
·
IF 2.467
Lan Cheng,Huiming Huang,Jingyou Zeng,Zulan Liu,Xiaoling Tong,Zhi Li,Hongping Zhao,Fangyin Dai
In this study, eight types of materials including nanoparticles (Cu and CaCO3), metallic ions (Ca2+and Cu2+), and amino acid substances (serine, tyrosine, sericin amino acid, and fibroin amino acid) were used as additives in silkworm diets to obtainin-situmodified silk fiber composites. The results indicate that tyrosine and fibroin amino acids significantly increase potassium content in silk fibers and induce the transformation of α-helices and random coils toβ-sheet structures, resulting in higher crystallinities and better mechanical properties. However, the other additives-modified silk fibers show a decrease inβ-sheet contents and a slight increase or even decrease in tensile strengths. This finding provides a green and effective approach to produce mechanically enhanced silk fibers with high crystallinity on a large scale. Moreover, the modification mechanisms of these additives were discussed in this study, which could offer new insights into the design and regulation of modified fibers or composites with desirable properties and functions.Keywords:silkworm silk;additives;secondary structure;thermal and mechanical properties
研究领域:
材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dong Zhang,Lei Zhao,Xiaohu Cui,Xinpeng Li,Zhisong Qian,Xueyan Zhou,Zhongren Ma,Mohd Sobri Takriff,Zhiqiang Li,Ying Niu,Guilan Ma,Gongtao Ding,Zifan Wang
Hydrogels with rapid wound-healing capabilities and antimicrobial effects are gaining significant interest in related fields. Nonetheless, developing a multifunctional hydrogel wound dressing with injectable self-assembling, self-healing, antimicrobial properties, and efficient skin wound-healing capabilities remained a formidable challenge. In this experiment, we drew inspiration from silkworm cocoons' natural formation and protective mechanisms, employing a novel physical cross-linking method to create an injectable and self-healing quaternary hydrogel successfully. The hydrogel is based on a matrix of silk fibroin/silk sericin (SF/SS), with 1,2-dimyristoyl-sn-glycero-3-phosphate sodium salt (DMPG) serving as a physical cross-linking agent to form the hydrogel network structure, and the incorporation of silver nanoparticles (AgNPs) further enhances its antimicrobial capabilities. Our biomimetic hydrogel, which replicated the chemical properties of silkworm cocoons, demonstrated excellent hydrophilicity with a water contact angle that ranged from 37 to 52°. Its tensile and compressive resistance was approximately four times greater than that of a pure SF hydrogel, and its swelling performance was about three times higher than that of a pure SF hydrogel. Furthermore, the hydrogel exhibited an impressive bacterial inhibition rate of over 98 % in bacterial growth and inhibition experiments, which provided a solid foundation for accelerating wound healing. Likewise, experiments with mice and histological analyses revealed that on day 7, the expression of TNF- α and IL-1 β in the wound tissues treated with the SF/SS/AgNPs hydrogel was significantly reduced by >25 % compared to the blank control group. This reduction indicates that the hydrogel could decrease the production of inflammatory cytokines, potentially aiding in the acceleration of wound healing and mitigation of inflammation-related adverse reactions. By day 14, the wounds were healed mainly, with the wound area reduced by 17 % compared to that of the blank group. This demonstrates the significant potential of this cocoon-mimetic hydrogel in accelerating wound healing and providing wound protection.
研究领域:
生物材料学
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医学工程
·
组织工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）