Emerging Contaminants
·
2025
·
IF 6.9
Wei Xiang,Fuhua Fu,Xuebo Shang,Jian Xu,Fengxiang Yu,Dengni Lai,Yang Shan
Dioxins are among the common persistent organic pollutants in crabs. To clarify the potential risk of dioxins pollution in Chinese mitten crab, eight types of samples were collected in the South Dongting Lake area for the analysis of 17 PCDD/Fs and 12 dl-PCBs. The results indicate that the concentration of Σ PCDD/Fs and Σ dl-PCBs in crabs was between 0.993–10.8 and 35.0–282 pg/g w.w., respectively. The mean WHO-TEQ value of Σ PCDD/Fs in crabs was 0.248 ± 0.205 pg TEQ/g w.w., and the mean WHO-TEQ value of Σ PCDD/Fs+dl-PCBs in crabs was 0.359 ± 0.280 pg TEQ/g w.w.. The total toxic equivalent of bottom sediment, surrounding soil, crab brown meat and juvenile crab were relatively high, which were 2.88, 2.67, 1.50 and 1.30 pg TEQ/g w.w., respectively. In the case of 99th percentile, the WHO-TEQ values of Σ PCDD/Fs and Σ PCDD/Fs+dl-PCBs in crab white meat were all within the maximum permitted levels as specified in commission regulation (EU) 2023/915. The HQ values of 99th percentile of Σ PCDD/Fs+dl-PCBs were 0.0783, 0.00703 and 0.0853 for eating brown meat products, eating white meat products and eating whole crabs, respectively. The coordinate points of the samples are connected to the origin by a straight line in the correspondence analysis map. The angle formed by the straight lines of crab brown meat/crab white meat and juvenile crab was acute angle, as did the feed. The angles between the straight line of bottom sediment/surrounding soil and that of other samples were all obtuse angles. In conclusion, although the dioxins of bottom sediment and surrounding soil are obvious, the dioxins of adult crabs are mainly related to that of the juvenile crabs and feed. At present, the intake of dioxins from Chinese mitten crab by Chinese residents is at a safe level because of low annual consumption. It is recommended that adults limit their weekly crab consumption to 1–4 crabs, or only 23.1–105 g of crab brown meat per week, or solely 435–1242 g of crab white meat per week.
研究领域:
环境科学
·
食品安全
✓ 已验证
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Mengting Huang,Yuan Ma,Qianru Fan,Shunli Che,Jun Zhang,Shuquan Ding,Shuren Zhu,Xilei Li
Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab ( Eriocheir sinensis ), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of Eriocheir sinensis to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 10 10 particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including CHT , CarL , and CarB , suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including PPARγ , GYK , PEPCK , and SCD , as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in E. sinensis by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of Eriocheir sinensis .
研究领域:
环境科学
·
生态毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUACULTURE NUTRITION
·
2024
·
IF 3
Xiaorong Huang,Ru Tan,Zhi Geng,Tao Zhang,Guangpeng Feng,Gang Yang,Feng Zhao,Ping Zhuang
A 60-day feeding trial was conducted to evaluate the impact of dietary Antarctic krill meal on the reproductive performance and embryo quality of the Chinese mitten crab, Eriocheir sinensis. Three diets were formulated, incorporating varying levels of Antarctic krill meal at 0% (Diet K0), 10% (Diet K10), and 20% (Diet K20), with a control group fed razor clam Sinonovacula constricta. Each diet was randomly assigned to three replicate tanks, each stocked with 5 males and 10 females. Male and female weights were 145.38 ± 8.01 and 102.57 ± 9.73 g, respectively. The results revealed no significant differences in weight gain rate, specific growth rate, and survival rate. However, the hepatopancreatic weight and hepatopancreas index of female crabs in each group decreased, while gonadal weight and gonadosomatic index increased significantly after 60 days, with Diet K20 showing the highest values. Egg production and fecundity of female crabs reached their peak in Diet K20, with no significant differences in reproductive indices among all groups. The phospholipid content in Diet K20 was significantly higher than in the other groups (). Cholesterol contents in Diet K0 and the control group were significantly higher than in Diet K10 and K20 (). No significant differences were observed in egg diameter, egg weight, moisture, crude protein, and crude fat between the groups. The content of C20 : 2 and C20 : 4n6 was highest in Diet K0, with a significant difference compared to Diet K10 (). However, no significant differences were found in the total content of saturated fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids among all groups. Based on the research findings, it is recommended that the optimal level of Antarctic krill meal in diets is 20%.
研究领域:
水产养殖学
·
动物营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 5
·4412
·
IF 5.6
Xinping Guo,Ziang Qian,Qiqi Pan,Yuqing Hu,Wangxin Mei,Xiumei Xing,Shaowu Yin,Jie Ji,Kai Zhang
Excessive use of antibiotics in aquaculture causes residues in aquatic animal products and harms human health. However, knowledge of florfenicol (FF) toxicology on gut health and microbiota and their resulting relationships in economic freshwater crustaceans is scarce. Here, we first investigated the influence of FF on the intestinal health of Chinese mitten crabs, and then explored the role of bacterial community in FF-induced intestinal antioxidation system and intestinal homeostasis dysbiosis. A total of 120 male crabs (48.5 ± 4.5 g) were experimentally treated in four different concentrations of FF (0, 0.5, 5 and 50 μg/L) for 14 days. Responses of antioxidant defenses and changes of gut microbiota were assessed in the intestine. Results revealed that FF exposure induced significant histological morphology variation. FF exposure also enhanced immune and apoptosis characteristics in the intestine after 7 days. Moreover, antioxidant enzyme catalase activities showed a similar pattern. The intestinal microbiota community was analyzed based on full-length 16S rRNA sequencing. Only the high concentration group showed a marked decrease in microbial diversity and change in its composition after 14 days of exposure. Relative abundance of beneficial genera increased on day 14. These findings illustrate that exposure to FF could cause intestinal dysfunction and gut microbiota dysbiosis in Chinese mitten crabs, which provides new insights into the relationship between gut health and gut microbiota in invertebrates following exposure to persistent antibiotics pollutants.Keywords:florfenicol;gut barrier;apoptosis;gut microbiota;crab
研究领域:
水产养殖
·
毒理学
·
微生物学
·
动物健康
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Wenlong Wan,Jie Xu,Jiayuan Shi,Xin Zhang,Anran Wang,Xiaojing Dong,Shuyan Miao
A 60-day feeding trial was conducted to determine the effects of the interaction of dietary niacin and lipid on the growth, antioxidant capacity and lipid metabolism in male Eriocheir sinensis . A total 162 crabs (initial weight, 33.27 ± 0.84 g) were fed with six semi-purified diets containing L 6 N 0 (6% lipid and 0 mg/kg niacin), L 6 N 400 (6% lipid and 400 mg/kg niacin), L 6 N 800 (6% lipid and 800 mg/kg niacin), L 12 N 0 (12% lipid and 0 mg/kg niacin), L 12 N 400 (12% lipid and 400 mg/kg niacin), L 12 N 800 (12% lipid and 800 mg/kg niacin), respectively. Elevated levels of niacin and lipid improved growth performance of crabs, but there were no significant differences. Crabs fed with 400 mg/kg niacin diets had higher hepatopancreas index (HSI) and lipase (LPS) than that of the other groups. Niacin promoted the accumulation of lipid in the hepatopancreas, but excessive accumulation led to more oxidative stress in crabs. In conclusion, excess lipid and niacin enhanced the growth performance of male Chinese mitten crabs by promoting the accumulation of lipid in the body at the expense of impairing their immune capacity. Crabs fed with 6% lipid and 400 mg/kg niacin diet had an advantage in lipid metabolism, without a significant decrease in antioxidant capacity, achieving a balance in growth performance and immunity.
研究领域:
水产养殖
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Environmental Chemistry and Ecotoxicology
·
2025
·
IF 8.2
Zhigang Yang,Jiani Liu,Chunyu Chen,Khor Waiho,Chenchen Zhang,Aqin Chen,Youji Wang
Microplastics (MPs) pollution and freshwater acidification represent emerging threats to aquatic ecosystems. The combined toxic effects of these stressors on crustaceans and their underlying immune-metabolic mechanisms remain poorly understood. This study investigated the individual and combined toxic effects of low pH and polystyrene MPs on the Chinese mitten crab ( Eriocheir sinensis ). Through a 21-day exposure experiment combined with enzyme activity analysis, gut microbiota profiling, and hepatopancreas metabolomics, the findings revealed: (1) Combined exposure to low pH (pH 6.5) and MPs exacerbated oxidative damage and immune suppression, demonstrating synergistic toxicity; (2) MPs alone primarily affected pyrimidine metabolism, whereas combined exposure significantly disrupted the tricarboxylic acid (TCA) cycle and arginine biosynthesis while activating serotonin metabolism; (3) Although the α-diversity of gut microbiota remained stable, its COG functional profile underwent significant alterations. The results indicate that freshwater acidification amplifies the toxic effects of MPs on crustaceans through immune-metabolic interactions, highlighting the urgency of assessing multiple environmental stressors in the context of climate change. The study provides novel mechanistic insights for aquatic ecological risk assessment.
研究领域:
环境科学
·
生态毒理学
·
分子生物学
✓ 已验证
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Fenju Qin,Tao Shen,Huixing Yang,Junchao Qian,Dan Zou,Jinlin Li,Hui Liu,Yunyi Zhang,Xuehong Song
Oxidative stress plays a crucial role in ammonia nitrogen toxicity. In this study, the beneficial effects of dietary nano cerium oxide (nano CeO 2 ) as a potent antioxidant were examined in the Chinese mitten crab ( Eriocheir sinensis ). Crabs were fed a diet supplemented with 0, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4, or 12.8 mg/kg nano CeO 2 for 60 d. The optimum supplementation level of nano CeO 2 that significantly increased weight gain rate and decreased feed coefficient was 0.8 mg/kg. This level also offered immune protection when crabs were kept under ammonia nitrogen stress and/or exposed to pathogen infection ( Aeromonas hydrophila ). Supplementation with 0.8 mg/kg of CeO 2 (i) relieved pathological damage to the hepatopancreas ; (ii) increased hemocyte counts, including total number of hemocytes, granulocytes , and hyalinocytes; (iii) decreased malondialdehyde content and increased antioxidant enzyme activities of superoxide dismutase and catalase in the hemolymph; (iv) increased the activities of lysozyme , acid phosphatase , and alkaline phosphatase in the hemolymph; and (v) increased gene and protein expression of cathepsin L in the hepatopancreas. Mortality increased when crabs were injected with bacteria under ammonia nitrogen stress, but dietary supplementation with 0.8 mg/kg nano CeO 2 decreased the mortality rate. Thus, the results of this study suggested that dietary supplementation with nano CeO 2 in crabs promoted growth and up-regulated immunity to bacterial infection under ammonia nitrogen stress.
研究领域:
水产养殖学
·
环境毒理学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Hao-kun Hua,Hui-xing Guo,Wen-bin Liu,Zi-shang Liu,Chao-fan He,Wan-ting Du,Yang-yang Huang,Wei Xiong,Li-ren Ding,Guang-zhen Jiang
This paper did research on the effect of dietary nicotinic acid (NA) on lipid metabolism and tryptophan metabolome of Chinese mitten crab ( Eriocheir sinensis ) to promote its growth. Three diets to administer to E. sinensis (37.25 ± 0.5 g), respectively, for 10 weeks were designed to contain 0% NA (control), 0.02% NA, and 0.10% NA. According to the results, when feeding crabs with 0.02% NA diet, the specific growth rate (SGR) increased markedly while the feed conversion ratio (FCR) was the opposite compared with 0% NA group. The content of total cholesterol (T-CHO) and low-density lipoprotein cholesterol (LDL) in hemolymph decreased significantly when feeding crabs with 0.02% NA diet while the content of high-density lipoprotein cholesterol (HDL) was the opposite. In addition, the relative expression level of microsomal triglyceride transfer protein ( mttp ) and kynureninase ( KFase ) decreased significantly in 0.10% NA group while the relative expression level of sterol regulatory element-binding protein 1 ( srebp-1 ) and nicotinamide adenine dinucleotide synthetase ( NADs ) increased significantly in 0.02% NA group. Meanwhile, the protein expression of 2-amino-3-carboxymuconic semialdehyde decarboxylase (ACMSD) in 0.02% NA group had a very significant increase compared with the other two groups. The result of tryptophan metabolome in hepatopancreas indicates that 0.02% NA in the diet could inhibit the downstream metabolic pathway of tryptophan. According to the results of this research, 0.02% NA in the diet can improve the growth and tryptophan metabolome through ACMSD and NADs , and lipid metabolism is also improved in this group, while 0.10% NA in the diet has no effect on crabs in growth performance.
研究领域:
水产养殖
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Biomass Conversion and Biorefinery
·
2025
·
IF 4.1
Cao Shuangming,Sun Jianguo,Liu Qian,Gu Yonghua
Crab shells, as the waste from seafood processing, are emerging as a valuable resource for recycling and environmental protection. By combusting the organic components of crab shells, the high-calcium crab shell ash (CSA) is produced. This ash serves as a promising raw material for CO 2 mineralization, contributing to energy conservation and emission reduction. In this study, the systematic mineralization experiment of CSA was conducted using wet carbonation, focusing on the effects of reaction temperature, liquid–solid ratio, particle size, and stirring speed on CO 2 mineralization. The optimal mineralization conditions were achieved at a temperature of 60 °C, a liquid–solid ratio of 10 mL/g, a stirring speed of 700 rpm, and a CSA particle size exceeding 200 mesh, resulting in a carbonation efficiency of 66.10%. Furthermore, we improved the mineralization process by modifying the CSA with Na 2 CO 3 and employing microwave treatment. The carbonation efficiency significantly increased to 71.80% with a Na 2 CO 3 concentration of 1.75 mol/L. Notably, microwave drying further enhanced the carbonation efficiency of CSA to 73.01%. Our findings contribute to a deeper understanding of CSA mineralization and establish a theoretical foundation and research direction for enhancing the carbon sequestration capacity and subsequent application performance of crustacean biomass ash.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 27
·期 4
·4520-4527
·
IF 2.914
Guo Feng,Zhu Zihan,Zheng Zhangqin,Jin Ying,Di Xiaoxuan,Xu Zhonghao,Guan Hongwei
Rampant use of tetracycline in animal feed is a threat to food security, the environment, and human health because of the risk of drug residues. Therefore, it is necessary to establish a sensitive, efficient, and reliable method for qualitative and quantitative detection of tetracycline. In this paper, we synthesized fluorescent carbon dots (FCDs) by thermal cracking of crab shell waste, and obtained a fluorescence quantum yield of 30%. Characterization of the FCDs by transmission electron microscopy, Fourier-transform infrared spectroscopy, ultraviolet visible absorption spectroscopy, and photoluminescence spectroscopy showed that they were fluorescent and evenly distributed with an average size of approximately 10 nm. We designed a sensitive probe for detecting tetracycline using the fluorescence intensity change of the FCDs. This method is sensitive, inexpensive, and environmentally friendly. The concentration of tetracycline was examined by comparing the fluorescence intensities of the FCDs before and after tetracycline addition. The limit of detection for tetracycline was 0.005 mg/L (signal-to-noise ratio = 3), which is promising for method development.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 15
·4720
·
IF 4.927
Xinjun Yu,Zengchao Jiang,Xiaodan Xu,Changyi Huang,Zheyi Yao,Xiao Yang,Yinjun Zhang,Dongsheng Wang,Chun Wei,Xuwei Zhuang
Chitin, the second richest polymer in nature, is composed of the monomer N-acetylglucosamine (GlcNAc), which has numerous functions and is widely applied in the medical, food, and chemical industries. However, due to the highly crystalline configuration and low accessibility in water of the chitin resources, such as shrimp and crab shells, the chitin is difficult utilize, and the traditional chemical method causes serious environment pollution and a waste of resources. In the present study, three genes encoding chitinolytic enzymes, including the N-acetylglucosaminidase fromOstrinia furnacalis(OfHex1), endo-chitinase fromTrichoderma viride(TvChi1), and multifunctional chitinase fromChitinolyticbacter meiyuanensis(CmChi1), were expressed in thePichia pastorissystem, and the positive transformants with multiple copies were isolated by the PTVA (post-transformational vector amplification) method, respectively. The three recombinants OfHex1, TvChi1, and CmChi1 were induced by methanol and purified by the chitin affinity adsorption method. The purified recombinants OfHex1 and TvChi1 were characterized, and they were further used together for degrading chitin from shrimp and crab shells to produce GlcNAc through liquid-assisted grinding (LAG) under a water-less condition. The substrate chitin concentration reached up to 300 g/L, and the highest yield of the product GlcNAc reached up to 61.3 g/L using the mechano-enzymatic method. A yield rate of up to 102.2 g GlcNAc per 1 g enzyme was obtained.Keywords:chitinase;shrimp and crab shell;N-acetylglucosamine;mechanoenzymology;heterologous expression
研究领域:
生物化学
·
生物技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MARINE POLLUTION BULLETIN
·
2023
·
IF 5.8
Yi-Lian Zhou,Wei-Ren Dong,Miao-An Shu
Benzophenones (BPs) are commonly used in personal care products like sunscreens and are increasingly being released into the environment, raising concerns about their potential ecotoxic effects. BPs as emerging environmental contaminants, little is known about their toxic effects on estuarine organisms . This study firstly investigated the toxic effects of five commonly used BPs on mud crabs ( Scylla paramamosain ). The crabs were exposed to varying concentrations of BPs for 14 days. The results showed that BPs caused damage to antioxidant systems in crabs. Transcriptome sequencing revealed that BP-3 and BP-1 had a greater impact on the crabs compared to the other BPs. Specifically, BP-1 and BP-3 caused severe damage to organelles and ribosomes. BP affected catalytic activity and hydrolase activity, BP-2 affected phosphoenolpyruate carboxykinase activity, and BP-4 affected tRNA aminoacylation and hydrolase activity. These findings can enhance our understanding of the ecotoxicity of BPs and may help to protect estuarine ecosystems .
研究领域:
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）