INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Qiuyang Huang,Xiaoling Zang,Zhiwei Zhang,Hang Yu,Baoyan Ding,Zhuangzhuang Li,Simin Cheng,Xin Zhang,Mustafa R.K. Ali,Xue Qiu,Zhihua Lv
The discovery of new anti-cancer drugs targeting the PD-1/PD-L1 pathway has been a research hotspot in recent years. In this study, biological affinity ultrafiltration (BAU), UPLC-HRMS, molecular dynamic (MD) simulations and molecular docking methods were applied to search for endogenous active compounds that can inhibit the binding of PD-L1 to PD-1. We screened dozens of potential cancer related endogenous compounds. Surprisingly, cyclic adenosine monophosphate (cAMP) was found to have a direct inhibitory effect on the PD-1/PD-L1 binding with an in vitro IC 50 value of about 36.4 ± 9.3 μM determined by homogeneous time-resolved fluorescence (HTRF) assay. cAMP could recover the proliferation of Jurkat T cells co-cultured with DU-145 cells and may suppress PD-L1 expression of DU-145 cells. cAMP was demonstrated to bind and induce PD-L1 dimerization by FRET assay, and also predicted by MD simulations and molecular docking. The finding of cAMP as a potential inhibitor directly targeting the PD-1/PD-L1 interaction could advance our understanding of the activity of endogenous compounds regulating PD-L1.
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EUROPEAN POLYMER JOURNAL
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2023
·
IF 6
Yi Yang,Yin Lu,Kan Zhang
Highly thermally stable polymeric materials synthesized from renewable resources are highly desirable in many high-performance fields due to the sustainable development of human beings. Herein, a new norbornene-containing monobenzoxazine thermosetting resin ( HPTM-fa ) exhibiting very high thermal stability has been prepared using natural bio-resourced tyramine and furfurylamine as starting materials. The structural details of HPTM-fa were characterized by FT-IR, NMR as well as high-resolution mass spectroscopies. The polymerization behaviors of cross-linkable norbornene , oxazine ring, and furan groups in HPTM-fa were investigated by DSC and in situ FT-IR analyses. In addition, the polybenzoxazine thermoset derived from this newly obtained bio-based mono-benzoxazine resin showed very high thermal stability with a T d5 of 413 °C and a glass transition temperature of 290 °C. The outstanding performance of thermoset based on HPTM-fa evidences its potential applications in the design of highly thermally stable polymeric materials.
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RSC Advances
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2022
·Vol. 12
·期 48
·31497-31505
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IF 4.036
M. R. Ali,M. S. Bacchu,D. D. Ridoy,P. L. Mozumder,M. N. Hasan,S. Das,M. F. H. Palash,S. Akter,N. Sakib,A. Khaleque,D. Chakrobortty,M. Z. H. Khan
In this study, hematite nanotube (HNT) and tyramine-based advanced nano-drug carriers were developed for inhibiting the growth of Klebsiella pneumoniae (K. pneumoniae). The HNT was synthesized by following the Teflon line autoclaved assisted hydrothermal process and tyramine was incorporated on the surface of the HNT to fabricate the formulated nano-drug. The nano-drug was prepared by conjugating meropenem (MP) on the surface of Tyramine-HNT and characterized using different techniques, such as scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared (ATR-FTIR), etc. Furthermore, the drug-loading efficiency and loading capacity were measured using a UV-vis spectrometer. The pH, amount of Tyr, and HNT required for drug loading were optimized. A controlled and gradual manner of pH-sensitive release profiles was found after investigating the release profile of MP from the carrier drug. The antibacterial activity of MP@Tyramine-HNT and MP was compared through the agar disc diffusion method which indicates that antibacterial properties of antibiotics are enhanced after conjugating. Surprisingly, the MP@Tyramine-HNT exhibits a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of K. pneumoniae lower than MP itself. These results indicate the nanocarrier can reduce the amount of MP dosed to eradicate K. pneumoniae.
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Biomass Conversion and Biorefinery
·
2023
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IF 4
Si Lin,Hou Juying,Lv Wei,Hou Qin,Xu Zhixiang,Ai Shiyun
In this paper, lignocellulose components were separated under mild conditions to achieve hierarchical utilization of biomass. Cellulose was retained in cellulose pulp with highly crystalline structure, and lignin powder was recovered, which offered a possibility for the high-value utilization of lignocellulosic biomass. The extracted lignin (EL) was carbonized and activated to prepare lignin-based porous carbon microspheres (LPCMs) and further used as a substrate to interact with fluorescein-5-isothiocyanate (5-FITC) for the detection of biogenic amines (BAs). The fluorescence of 5-FITC was found to be quenched in the presence of LPCMs. And then, upon addition of BAs, 5-FITC was released from the surface of LPCMs, which resulted in the fluorescence recovery of 5-FITC. Thus, a novel “turn off–on” fluorescent probe was constructed for sensitive and visual detection of BAs. Meanwhile, the retained cellulose skeleton structure was used to prepare a smart identification label for real-time and visual evaluation of the freshness of pork and shrimp. This work realized the improvement of comprehensive utilization rate of lignocellulose and developed a rapid, efficient, and sustainable method which combined natural lignocellulose materials with green analytical technology for visually evaluating food quality.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
·
IF 8.025
Yikun Ren,Shanshan Ma,Dan Zhang,Shen Guo,Rong Chang,Yuanmeng He,Minghao Yao,Fangxia Guan
Infected wound healing has always been a challenge in clinic. Effective and economic wound dressings with combined antibacterial activity and pro-healing function are highly desirable, especially in the context of infected wounds. An obvious advantage of antibacterial wound dressing is to avoid the overuse of antibiotics and the occurrence of drug resistance. Herein, an injectable hyaluronic acid hydrogel with antioxidative and photothermal antibacterial activity as a functional dressing was prepared, characterized and evaluated in an experimental infected wound model. This hydrogel was developed by loading graphene oxide (GO) in a natural polymer network consisting of hyaluronic acid grafted with tyramine (HT) and gelatin grafted with gallic acid (GGA). The HT/GGA/GO hydrogels have a porous cross-linked network structure and demonstrate a good stability, biocompatibility, antioxidant, hemostatic and photothermal antibacterial activity against Escherichia coli and Staphylococcus aureus . In addition, in vivo studies have shown that HT 1 /GGA 2 /GO 0.1 hydrogel dressing combined with photothermal therapy can effectively prevent early infection and accelerate wound healing. These results indicated this functionalized injectable hydrogel HT 1 /GGA 2 /GO 0.1 has a great potential in wound dressing application.
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Xu Xu,Jiaxin Gao,Di Cao,Xiang Li,Xin Zhao,Shuang Yue,Lei Zhang
In this paper, the novel 3D N-doped magnetic porous carbon spheres (3D N-MPCSs) was constructed via a simple template-free and self-doping method, followed by a direct carbonization of polymer with Fe 3+ for simultaneous activation and magnetization. For the first time, the designed 3D N-MPCSs was used as multifunctional (for extraction and magnetic separation) adsorbents for microwave-assisted derivatization and magnetic solid phase extraction, which exhibited excellent extraction ability for its large surface area, mesoporous structure, spherical morphology, and incorporation of N/O heteroatoms. Moreover, when the microwave-assisted derivatization was combined with the magnetic solid phase extraction, the extraction, derivatization and concentration was completed simultaneously within 135 s. Furthermore, this method has a low detection limit (0.059–0.073 ng mL −1 ) and high enhancement factors (333 < EFs < 382) for trace biogenic amine (BAs). The recoveries were between 92.3 and 108.8% with relative standard deviations less than 5.9%. Overall, a simple, rapid, efficient and sensitive method for the analysis of BAs in food samples was developed, which provided the basis for routine detection of food safety.
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NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 9
·4156-4161
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IF 3.925
Yi Wang,Chengpei Du,Ze Liu,Kanglin Pei,Yan Zhang,Wenjing Qi
A lucigenin and riboflavin chemiluminescence system was utilized for the first time to achieve the “turn-on” detection of tyrosinase activity via an in situ reaction between tyrosinase and tyramine to generate dopamine. Tyramine is the model substrate of tyrosinase and dopamine is the product. Since dopamine can enhance the chemiluminescence of the lucigenin and riboflavin system, the chemiluminescence of lucigenin and riboflavin can be enhanced in the presence of both tyramine and tyrosinase. In the presence of 6.0 U mL−1 tyrosinase, the chemiluminescence intensity was nearly 10 times higher than that of lucigenin, riboflavin and tyramine mixtures. Therefore, this method has been applied in the chemiluminescence “turn-on” detection of tyrosinase activity in the range of 0.1–8.0 U mL−1 with the limit of detection (LOD) of 0.028 U mL−1. This overall process of sensing tyrosinase activity took nearly 30 min. Moreover, it had high selectivity in the chemiluminescence “turn-on” detection of tyrosinase activity towards other enzymes, such as glucose oxidase, lysozyme, trypsin or alkaline phosphatase. When 1.0, 2.0 and 5.0 U mL−1 tyrosinase were added to the diluted serum samples, recoveries were in the range of 100.2%–104.1%. This indicates that this method for the chemiluminescence “turn-on” detection of tyrosinase activity has high sensitivity, selectivity, accuracy and fast response time.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Fei Li,Yunlu Wei,Jing Zhao,Luyao Zhang,Quanhong Li
Orally administrated is the primary way of pumpkin polysaccharides intake, and this is the way that they elicit their multiple bioactivities. However, little is known about how those orally ingested polysaccharides work, and the pharmacokinetics of pumpkin polysaccharides after orally administrated has not been described. This study aimed to elucidate the pharmacokinetic information (the plasma concentration-time curve, tissue distribution, and excretion profiles) of the pumpkin polysaccharide (PPc) in vivo after oral administration. Results revealed that PPc could enter into the blood and exhibited a relatively long circulation in the blood with a mean residence time (MRT) of 7.45 h and presented a higher aggregation of PPc in the liver and kidneys. To obtain the visualization of the systemic circulation of PPc, in vivo imaging was used with near-infrared fluorescence (NIRF) imaging, which distributed into various tissues with different region of interest (ROI) values after oral administration. The in-depth understanding of oral delivery of PPc in vivo was provided, which will provide the instruction clinical medication and deepen the understanding of bioactivities mechanisms of oral pumpkin polysaccharides.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yujuan Qin,Pengcheng Huang,Fang-Ying Wu
With food safety problems abounding, it is essential to explore some efficient and effective methods for monitoring food quality. To this end, we have developed a histamine-responsive carbon dots (CDs)-based sensing system for visual monitoring of food spoilage. In this system, the aldehyde group on the surface of CDs serves as the recognition sites to combine with the amino group on histamine to form a Schiff base that effectively enhances the fluorescence. On the other hand, 1-aminoanthraquinone (ANQ) was introduced as a reference signal to endow the fluorescent probe with self-calibrating capacity. As the histamine concentration increases, linear ratiometric response was obtained accompanied with a distinguishable fluorescence color change from red to blue. Besides, test strips-based smartphone visual assays were successfully used to evaluate and monitor the histamine levels in fish samples. Our proposed sensing strategy has been demonstrated to be efficient and robust along with great potential for real-time visual monitoring of food spoilage.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Tianhang Weng,Zejian He,Zhaohua Zhang,Yulong Chen,Mi Zhou,Bianying Wen
In the present work, we constructed a methodology to graft starch with special groups, such as alkyl, phenolic, naphthalimide derivatives (ND) and polymer, by a simple reaction under generally mild conditions without catalysts or UV irradiation , based on precursor starch acetoacetate (SAA). The completeness of these reactions has been proved to be ideal. After grafting, the starch derivatives have some common changes, for instance, their solubility is improved in certain solvents. On the other hand, the introduction of different functional groups will also bring some characteristics to the derivatives (e.g. ND brings fluorescence). In addition, part of the derivatives shows excellent machinability, and their hot-pressed samples exhibit great transparency and mechanical strength. Specially, the alkyl grafted starch displays excellent toughness, properties of deformation and self-recovery. In conclusion, this method has good universality and methodological significance, and offers insights into the larger-scale industrial application of starch.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 19
·11087
·
IF 6.208
Zhuangzhuang Li,Baoyan Ding,Mustafa R. K. Ali,Lizhen Zhao,Xiaoling Zang,Zhihua Lv
Abnormal tryptophan metabolism is linked to cancer and neurodegenerative diseases, and tryptophan metabolites have been reported as potential prostate cancer (PCa) biomarkers. However, little is known about the bioactivities of tryptophan metabolites on PCa cell growth. In this study, MTT and transwell assays were used to study the cytotoxicities of 13 major tryptophan metabolites on PCa and normal prostate epithelial cell lines. Ultraperformance liquid chromatography–high resolution mass spectrometry (UPLC–HRMS) was used to analyze metabolic changes in cells treated with tryptamine. Flow cytometry, confocal imaging, and Western blot were used to test the apoptosis induced by tryptamine. It was shown that tryptamine had obvious inhibitory effects on PCa cell lines PC-3 and LNCaP, stronger than those on the normal prostate cell line RWPE-1. Tryptamine was further shown to induce apoptosis and inhibit PC-3 cell migration. Metabolic changes including amino acid metabolism related to cell proliferation and metastasis were found in PC-3 cells treated with tryptamine. Furthermore, a PC-3 xenograft mouse model was used to study the effect of tryptamine in vivo. The intratumoral injection of tryptamine was demonstrated to significantly reduce the tumor growth and tumor sizes in vivo; however, intraperitoneal treatment resulted in increased tumor growth. Such dual effects in vivo advanced our understanding of the bioactivity of tryptamine in regulating prostate tumor development, in addition to its major role as a neuromodulator.Keywords:tryptamine;prostate cancer (PCa);cell proliferation;apoptosis;cell migration;xenograft model antitumor assays
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Jianfeng Yan,Quanbin Fu,Shikai Zhang,Yu Liu,Xianbao Shi,Juying Hou,Junling Duan,Shiyun Ai
A novel dual-emission ratiometric fluorescent sensor for biogenic amines (BAs) was prepared by simple mixing blue fluorescent carbon dots (CDs) and yellow fluorescent CdTe quantum dots (CdTe QDs). Based on different sensitive properties of pH, CdTe QDs and CDs were used as the response signal and internal reference signal, respectively. The developed ratiometric fluorescent sensor achieved quantitative analysis of eight kinds of BAs with rapid response (30 s) and low limits of detection (1.259–5.428 μM). Furthermore, color-tunable fluorescent test strips were constructed by easily assembling CDs and CdTe QDs onto filter paper. The obtained smart label showed a distinguishable fluorescent color variation from blue to green during the corruption of shrimp samples. The smart label with advantages of convenience and rapidness provided a method for visually monitoring the freshness of food samples.
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