PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
·
2020
·
IF 1.646
Yan Jin Gao,Wei Jie Fu,Jia Liu,Yi Juan Chen,Guang Hui Dai
Cucumber powdery mildew (PM) is a destructive fungal disease which has a substantial impact on cucumber yield and quality. Our previous work has demonstrated that (+)-( S )-ar-turmerone (ATM) is an effective compound for controlling PM infected by Podosphaera xanthii in cucumber. In this study, the effect of ATM on cucumber PM was histologically investigated by observations on the morphology of the pathogenic fungus at different time points after inoculation. It was found that the ATM caused the collapse of the pathogen's conidia and mycelia structure, the cytoplasm coagulation, the reduction of the conidia germination rate, and the inhibition of the conidiophore growth, therefore, prevented the re-infection of the P. xanthii . Evaluation of the content of total phenols and flavonoids in cucumber ATM-treated leaves showed that the ATM-treatment could increase the content of total phenols and flavonoids in cucumber leaves significantly in comparison to the control. A further analysis of the contents of the compounds showed that ATM-treatment could increase the content of rutin , quercetin , hesperetin , and gallic acid , syringic acid , vanillic acid and salicylic acid. Meanwhile, the activity of phenylalanine ammonia-lyase (PAL) and polyphenol oxidase (PPO) in cucumber leaves were increased slightly, but the activity of peroxidase (POD) and superoxide dismutase (SOD) were significantly increased through ATM treatment. It should be estimated from this study that the ATM not only directly affected the growth of P. xanthii but also enhanced the cucumber plant resistance to the disease.
研究领域:
植物病理学
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植物化学
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 6
·066509
·
IF 3.721
Xia Qiu,Xiang Qing,Wang Sufan,Yinggui Zhu
4-Chromanone and chromone, these two structurally similar pharmaceutical intermediates play a vital role in drug synthesis, but their related electrochemical properties have not been reported. In this work, we have found that 4-chromanone and chromone can generate strong electrochemiluminescence (ECL) during their reduction by using K2S2O8 as co-reactant in H3BO3-Na2B4O7 buffer solution. The ECL reaction mechanism has been investigated carefully by studying their fluorescence (FL) spectrum, ECL spectrum, and the important role of co-reactants in the system. Moreover, it has been found that iodide ion (I−) can inhibit the ECL signal of 4-chromanone and the ECL decrease degree is related to the amount of added I−. Therefore, a new ECL method for the detection of iodide ion has been established with high sensitivity, good selectivity and wide linear range, which can be used for detecting I− in real samples.
研究领域:
分析化学
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药物化学
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DOI
高影响因子
PHARMACEUTICAL BIOLOGY
·
2017
·
IF 1.916
Xu-Xin Zhang,Yun-Feng Cao,Li-Xuan Wang,Xiao-Lin Yuan,Zhong-Ze Fang
Contents: Danshen is a popular herb employed to treat cardiovascular and cerebrovascular diseases worldwide. Danshen–drug interaction has not been well studied.Objective: The inhibitory effects of four major tanshinones (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) on UDP-glucuronosyltransferases (UGTs) isoforms were determined to better understand the mechanism of danshen–prescription drugs interaction.Materials and methods: In vitro recombinant UGTs-catalyzed 4-methylumbelliferone (4-MU) glucuronidation reaction was employed. Tanshinones (100 μM) was used to perform the initial screening of inhibition capability. High-performance liquid chromatography (HPLC) was used to separate 4-MU and its glucuronide. In vitro–in vivo extrapolation (IV–IVE) was employed to predict in vivo inhibition situation.Results: Cryptotanshinone inhibited UGT1A7 and UGT1A9 with IC50 values of 1.91 ± 0.27 and 0.27 ± 0.03 μM, respectively. Dihydrotanshinone I inhibited UGT1A9-catalyzed 4-MU glucuronidation reaction with the IC50 value of 0.72 ± 0.04 μM. The inhibition of cryptotanshinone towards UGT1A7 and UGT1A9 was best fit to competitive inhibition type, and UGT1A9 was non-competitively inhibited by dihydrotanshinone I. Using in vitro inhibition kinetic parameters (Ki) and in vivo maximum plasma concentration (Cmax) of cryptotanshinone and dihydrotanshinone I, the change of area-under-the-concentration–time curve (AUC) was predicted to be 0.4–4.2%, 3.7–56.3%, and 0.6–6.4% induced by cryptotanshinone and dihydrotanshinone inhibition towards UGT1A7 and UGT1A9, respectively.Discussion and conclusion: The inhibitory effects of tanshinones towards important UGT isoforms were evaluated in the present study, which provide helpful information for exploring the mechanism of danshen–clinical drugs interaction.
研究领域:
药理学
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中药学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
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2021
·
IF 11.236
Lixiao Wang,Yajie Zheng,Yiran Zhou,Junhe Lu,Jean-Marc Chovelon,Yuefei Ji
Ketoprofen (KTF) is a nonsteroidal anti-inflammatory drug frequently detected in natural and engineering waters. Because KTF is particularly photolabile (half-life ∼4 min), knowledge of the fate and ecological risks of KTF photoproducts in the aquatic environment is especially essential. Herein, we systematically investigated the photophysics, photochemistry , and photosensitization of KTF photoproducts in aqueous solution under 365 nm irradiation (UV 365 ). Results show that KTF photolyzed rapidly and formed 3-ethyl- α -hydroxylbenzophenone (EtOH-BP), 3-ethyl- α -hydroperoxylbenzophenone (EtOOH-BP), 3-acetylbenzophenone (AcBP), and 3-ethylbenzophenone (EtBP), as identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-resolution mass spectrometry (HRMS). The presence of O 2 significantly affected the evolution of photoproducts during KTF photolysis. The photophysical properties of EtBP and AcBP were characterized by spectroscopic approaches. In particular, transient absorption spectra obtained by nanosecond laser flash photolysis (LFP) indicated that EtBP and AcBP were excited to triplet states with lifetimes of 28 and 2.4 µs, respectively. EtBP underwent further photodegradation , giving rise to EtOH-BP, EtOOH-BP, and AcBP upon UV 365 irradiation. The reaction is proposed to proceed through an excimer precursor ( 3 [EtBP···EtBP]*) followed by intramolecular H-abstraction. In contrast, AcBP was relatively photostable, particularly under aerated condition. Both EtBP and AcBP have strong photosensitizing activity, as evidenced by the triplet probe 4-( N,N -dimethylamino)benzonitrile (DMABN). ECOSAR program suggested that the photoproducts are more ecotoxic and bioaccumulative than the parent KTF. Results of this study underscore the need to scrutinize the formation and fate of KTF photoproducts in sunlit surface waters.
研究领域:
环境科学
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光化学
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毒理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 9
·1733-1740
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IF 4.3
Tang Kexin,Dong Jiacheng,Zheng Zhengheng,Zhang Ting,Pan Huayi,Jia Honghua,Li Yan,Wei Ping
ω-Transaminases (ω-TAs) are widely available for the production of chiral amines and unnatural amino acids. Herein, a rapid spectrophotometric method was developed for screening ω-TAs based on the colored products that can be generated from transamination reactions between aliphatic α-diketones and amino donors catalyzed by ω-TAs. The possible mechanism of the formation of the colored product was investigated according to LC-Q-TOF–MS analysis. Among seven diketones, 2,3-butanedione was selected as the most suitable amino acceptor for colorimetric screening of ω-TAs with high efficiency, high sensitivity, and low background interference. Meanwhile, the absorbance of the colored product generated by 2,3-butanedione catalyzed by ω-TAs in this method was linearly correlated with the results by HPLC analysis. This method was also confirmed to effectively screen ω-TA mutants with high activity towards isopropylamine. Graphical Abstract
研究领域:
生物化学
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酶工程
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2020
·Vol. 58
·期 9
·880-886
·
IF 1.28
Li Xiao,Yuan Tao,Zhao Ting,Wu Xiaomei,Yang Yaling
An environmentally friendly method for the determination of testosterone and methyltestosterone by acid–base-induced deep eutectic solvents liquid–liquid microextraction (DES-ABLLME) combining with high-performance liquid chromatography was established. The deep eutectic solvent (DES) consisting of menthol:lauric acid:decanoic acid (3:1:1) can act as both hydrogen bond donor and hydrogen bond acceptor. In this approach, ammonia solution (NH3•H2O) is used as an emulsifier to react with DESs in the extraction process to generate salt and form milky white solution, achieving high extraction efficiency. Hydrochloric acid was used as a phase separator to change the emulsification state and promote the separation of extraction agent from water phase. A series of parameters were optimized including the volume of DES and the emulsifying agent, glucose concentration as well as hydrochloric acid volume. The method was linear in the range 0.5–100 μg mL−1 with a correlation coefficient (R) of 0.9999, and the limits of detection were 0.067 and 0.2 μg mL−1 for testosterone and methyltestosterone, respectively. This method was applied to analyze testosterone and methyltestosterone in milk samples, and the recoveries were between 89.2 and 108.2%.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2014
·Vol. 49
·期 10
·2290-2298
·
IF 1.354
Yurong Ma,Huihua Huang
Summary To investigate the relationship between bioactive compounds and antioxidant property, total phenolic content (TPC), total flavonoid content (TFC), individual and total isoflavone content of soymilk and their correlations with oxygen-radical-absorbing capacity (ORAC), ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity values were evaluated and compared. Results showed that TPC, TFC, isoflavones (including concentration and profiles) and antioxidation activity significantly varied among ten tested soybean cultivars. Significant correlations were established between DPPH and TFC ( r = 0.553, P < 0.01), DPPH and TPC ( r = 0.753, P < 0.01), FRAP and TFC ( r = 0.599, P < 0.01) and FRAP and TPC ( r = 0.616, P < 0.01). Positive linear correlations were found between subtotal, total isoflavones and ORAC. Unlike other isoflavone monomers, aglycone isoflavones correlated positively with DPPH and FRAP significantly. DPPH well correlated with FRAP, whereas neither DPPH nor FRAP correlated with ORAC. TPC, TFC, individual and total isoflavone values are potentially useful for soymilk antioxidant activity assessment.
研究领域:
食品科学
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营养学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Mingying Ma,Tianhao Wu,Zhian Hu,Gongwei Sun,Sichun Zhang
High-throughput sample preparation is important in clinical mass spectrometry (MS) analysis. In this work, we developed a method by coupling Nano-ESI-MS with magnetic SPE using a MOF material, Fe 3 O 4 @UiO-66, as sorbent for simultaneous determination of testosterone (T) and androstenedione (4-AD) in serum samples. The proposed method presented good linearity in the range of 0.1–5 μg/L and the recoveries of serum samples were 90.7%-110.0% and 101.7%-108.7% for T and 4-AD, respectively. The results indicated that the Fe 3 O 4 @UiO-66 is an ideal sorbent for androgens extraction and the method is reliable for high-throughput quantitation of T and 4-AD in clinical applications.
研究领域:
分析化学
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临床医学
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DOI
高影响因子
Journal of Flow Chemistry
·
2018
·Vol. 8
·期 1
·29-34
·
IF 1.658
Yu Jiangang,Xu Jianguo,Li Jie,Jin Yi,Xu Wanghui,Yu Zhiqun,Lv Yanwen
A two-step flow reactor has been set up for an expeditious synthesis of 4-benzylidene-pyrazol-5-one derivatives. This procedure involved cyclization of aromatic hydrazine/ β -keto ester and subsequent Knoevenagel condensation reaction with aromatic aldehydes in tandem without isolation of intermediates. Residence time was reduced to less than 2 min in total through conducting this reaction at a relatively high temperature. Accordingly, 15 samples were achieved and isolated in moderate to excellent yields.
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研究领域:
有机化学
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药物化学
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DOI
高影响因子
PLoS One
·
2021
·Vol. 16
·期 9
·e0255504
·
IF 3.24
Jing Zhang,Zhou-Tao Pei,Ya-Ni Zhao,Meng Zhang,Li-Ling Zhang,Wen-Qiang Wang,Jing-Ya Wu,Ran Yu,Li-Wei Sun
Benzophenone (BPs) and 4-Methylbenzylidene Camphor are used as ultraviolet (UV) filters to protect the skin and hair in personal care products. The discharging of the three chemicals may endanger the receiving water ecosystem. In the present study, the mutagenicity of BP-6, BP-8, and 4-Methylbenzylidene Camphor was tested using the Salmonella typhimurium reverse mutation test (Ames test) in the system with and without rat liver microsomal preparations (S9). Four S . typhimurium strains, TA97, TA98, TA100, and TA102 were employed in the Ames tests. The mutagenicity was detected from all three chemicals. The addition of S9 increased the mutation ratios of three chemicals to four strains, except BP-6 to TA100 strain and 4-MBC to TA97 and TA98 strain. In the mixed experiment, all positive effects were detected in the absence of S9. However, the results all became negative in the presence of S9. For the mixture of BP-6 and 4-MBC, positive results were detected on four tester strains except for the TA100 strain. For the mixture of BP-6, BP-8, and 4-MBC, positive results were detected on four strains. The mixture test results showed antagonism in mutagenicity for the mixture of BP-6 and 4-MBC to TA98 and TA100 strains and the mixture of BP-6, BP-8, and 4-MBC to TA100 and TA102 strains.
研究领域:
环境科学
·
毒理学
·
生物学
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DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hongxia Tan,Hongyuan Zhou,Ting Guo,Yuhao Zhang,Jiaxin Li,Chi Zhang,Liang Ma
Food processing might induce the transformation of hidden ZEN (zein-bound ZEN) in maize. The objective of this study was to assess the effect of processing factors on free ZEN and hidden ZEN. After zein was treated under different temperature and pH, ZEN was quantified in samples before and after in vitro digestion. The ratios of hidden to total ZEN in zein are decreased from 54.25% to 40.74% after thermal treatment and from 54.25% to 0 after alkaline treatment, respectively. Conversely, acid treatment increased the ratio of hidden to total ZEN from 54.25% to 100%. Thus, it can be concluded that thermal or alkaline condition induced the conversion of hidden ZEN to free ZEN while acid condition promoted the ZEN-zein interactions to form the hidden ZEN. Overall, temperature and pH values played a vital role in the conversion of hidden ZEN during food processing.
研究领域:
食品科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2018
·
IF 2.631
Wentian Li,Nan Zhang,Ali Farajtabar,Xingang Feng,Gaoquan Chen,Xinbao Li,Hongkun Zhao
Experimental measurement of drug quinocetone solubility in water-co-solvent mixtures of ethanol, isopropanol, dimethyl sulfoxide (DMSO) and N,N -dimethylformamide (DMF) were determined by the static equilibrium method at temperature range of (283.15–328.15) K under ambient condition (101.2 kPa). The solid phase equilibrated with liquor was examined by X-ray power diffraction, resulting in no polymorphic transformation, solvate formation or crystal transition compared to the raw material. Quantitative knowledge about the solvent effect on the solubility was obtained with the help of linear solvation energy relationships in the terms of dipolarity-polarizability, hydrogen-bond donor and acceptor ability, and cavity term of mixtures. The preferential solvation was investigated from their thermodynamic solution properties by means of the inverse Kirkwood-Buff integrals. The preferential solvation parameters ( δx 1,3 ) for ethanol, isopropanol, DMSO or DMF were negative in the four co-solvent mixtures with water-rich compositions, which indicated that quinocetone was preferentially solvated by water. Temperature has a little effect on the preferential solvation magnitudes for the ethanol and isopropanol mixtures. The higher solvation by water could be explained in terms of the higher acidic behavior of the solvents interacting with the Lewis basic groups of the quinocetone. In addition, thermodynamic co-solvency models including the Jouyban-Acree model, van’t Hoff-Jouyban-Acree model and Modified Apelblat-Jouyban-Acree model were employed to describe the solubility of this drug obtaining average relative deviations lower than 2.14% and root-mean-square deviations lower than 1.75 × 10 −4 for correlative studies. The standard dissolution enthalpies of quinocetone in the solvent mixtures were positive, demonstrating that the dissolution process of quinocetone was endothermic.
研究领域:
药学
·
物理化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）