COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
·
IF 4.539
Yumeng Lian,Yi Wang,Dun Zhang,Likun Xu
Developing a stable and reusable material for antibacterial application in an enzyme-like system is still a challenge. Herein, Mn–Co–S–Se (MnCo 2 S x Se 4–x )/Ni foam (NF) are synthesized by introducing S 2– and Se 2– in MnCo 2 O 4 /NF with a facile hydrothermal approach. With the different molar ratios of S/Se, the surface morphology of MnCo 2 S x Se 4–x /NF shows different growth and the catalytic activity also presents regular changes. MnCo 2 S 3.1 Se 0.9 /NF possesses a high enzyme-like ability with higher V max than natural horseradish peroxidase . Due to the outstanding peroxidase-like activity, MnCo 2 S 3.1 Se 0.9 /NF also exhibits broad-spectrum bactericidal performance towards Escherichia coli and Staphylococcus aureus which stems from the formation of ·O 2 – from H 2 O 2 . In the reutilization experiment, the enzyme-like activity keeps a stable value after 25 cyclic tests and the bactericidal performance is still stable after 10 cyclic tests, proving that MnCo 2 S 3.1 Se 0.9 /NF possesses relative stability and reusability. This research showed the great prospect of new energy and environmental catalysis.
研究领域:
生物材料学
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环境催化
·
纳米酶研究
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2019
·
IF 2.12
Yun Li,Yueqin Li,Xiaohui Liu,Yong Yang,Dong Lin,Qinwei Gao
Two Co(II) complexes, Co(BBH)phenMeCN (1) and Co(BSH)phenMeCN (2) (HBBH = benzophenone benzoyl hydrazone, HBSH = benzophenone salicylylhydrazone ligand and phen = 1,10-phenanthroline) were prepared and characterized by single X-ray crystallography. The investigation of the complexes binding with herring sperm DNA (HS-DNA) revealed that stronger binding interaction exists between the complexes and HS-DNA compared to the corresponding free ligands. And both complexes can bind to DNA through intercalation of the phenyl rings between adjacent base pairs in the double helix. Meanwhile, bovine serum albumin (BSA) binding studies reveal the complexes can effectively interact with BSA and change the secondary structure of BSA. Superoxide dismutase (SOD) mimic activity of the complexes was investigated and the results showed that complex 2 showed a stronger radical scavenging potency against O 2 .- radical with an IC 50 value of 16.10 μM. The catecholase-like activity of both complexes has been investigated with 3,5-di- tert -butylcatechol (3,5-DTBC) as the substrate in methanol solution under completely aerobic conditions. The catalytic reaction follows Michaelis-Menten enzymatic reaction kinetics with turnover numbers ( k cat ) of 34.14 h −1 and 30.36 h −1 for complex 1 and complex 2, respectively.
研究领域:
配位化学
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生物无机化学
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药物化学
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产品关联
DOI
高影响因子
Journal of Materiomics
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2022
·
IF 8.589
Wei Lu,Shuhan Chen,Haifeng Zhang,Jiajun Qiu,Xuanyong Liu
H 2 O 2 and glutathione (GSH) are critical redox molecules in the organism. Abnormal levels of cellular H 2 O 2 and GSH are closely related to some diseases. Thus, it is imperative to detect H 2 O 2 and GSH efficiently. In this work, Fe N C single atom nanozymes (SANs) with both peroxidase and oxidase-mimicking activities were successfully prepared with the help of formamide condensation by one-step hydrothermal method. The Fe N C SANs show excellent peroxidase-like activity, which possess a higher affinity for H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB) than horseradish peroxidase (HRP). Then, based on the chromogenic reaction of TMB, a colorimetric biosensor to detect H 2 O 2 and GSH was developed. This biosensor has the linear ranges of 10–600 μmol/L for H 2 O 2 with a low detection limit of 4.360 μmol/L and 100–400 μmol/L for GSH with a low detection limit of 78.33 μmol/L. Besides, this colorimetric biosensor exhibited a good recovery of H 2 O 2 and GSH in diluted human serum. Finally, the Fe N C SANs were encapsulated into agar gel to self-quantitatively detect GSH by naked eyes. This work provides a non-pyrolytic way to prepare SANs, which broadens the synthetic method of SANs and may promote the development of SANs for biosensor application.
研究领域:
生物化学
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医学诊断
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纳米材料科学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 8
·2016-2024
·
IF 6.331
Yuzhen Li,Hairihan Zhou,Tongtong Li,Xiaoxia Jian,Zhida Gao,Yan-Yan Song
Nanomaterial-based artificial enzyme mimetics have attracted increasing attention because of their robust stability, adjustable activity, and cost-effectiveness. In this study, we developed a simple and effective method for the synthesis of highly dispersed ultrafine PdCo alloys with peroxidase- and catalase-like activities. The aberration-corrected transmission electron microscopy analysis verified that the cyanogel precursor in the mesoporous silica nanospheres (MSNs) was converted to PdCo alloy in NH3 at a high temperature. The PdCo alloy was homogenously distributed in MSNs as ultrafine and monodispersed particles. By selectively removing the Co species from the binary alloy through an acid-leaching approach, the role of each component in the enzyme-like mimetics was systematically studied. Using glutathione (GSH) as the model analyte, the potential application of PdCo@MSNs in GSH detection from complex cell media was confirmed via colorimetric assay. The ultrafine alloy size, double mimetic activities, and abundant loading space of PdCo@MSNs make them promising not only in clinical diagnosis but also in overcoming hypoxia-induced photodynamic therapy resistance in tumor treatment.
研究领域:
纳米材料
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生物医学工程
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仿生催化
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Yijuan Long,Wen Zheng,Danyang Yi,Yadi Pan,Huzhi Zheng
Ficin has dual enzyme activity , i.e., protease and peroxidase-like activity. In some respects, its application is limited by the protease activity of ficin. Herein, we used tris (2-carboxyethyl) phosphine hydrochloride (TCEP) to break the three pairs of disulfide bonds of ficin, and then blocked the free thiol groups with N-ethylmaleimide (NEM) to synthesize ficin-TN. The results showed that ficin-TN had increased peroxidase-like activity and reduced protease activity. According to this phenomenon, we have exploited a colorimetric method with high sensitivity and selectivity for the one-step detection of glucose. Comparing with ficin, ficin-TN has wider detection range (0.1–300 μM) and lower detection limit (88 nM), and our method is simpler and more timesaving than other two-step methods. Furthermore, the actual appliances of ficin-TN for glucose detection in human serum have been illustrated with satisfied result, suggesting that its promising utilization in various fields.
研究领域:
生物化学
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分析化学
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酶学
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产品关联
DOI
PubMed
高影响因子
Microbial Biotechnology
·
2025
·Vol. 18
·期 3
·e70130
·
IF 5.2
Kailin Shao,Xiaobin Yu,Yan Zhao,Ying Zhang,Xiaobo Liu
Organic selenium, a bioavailable form of the essential trace element selenium, holds significant potential for improving human health through dietary supplements and functional foods. However, low bioconversion efficiency has primarily limited the biosynthesis of organic selenium compounds. Here, we focused on enhancing the biosynthesis of organic selenium by optimising the expression and activity of two key enzymes, SenB and SenC, involved in the conversion process. We compared several expression systems, including fusion expression and dual-promoter approaches, and optimised reaction conditions such as temperature, pH and incubation time. Our results showed that mutations of SenC more than doubled enzyme activity, resulting in a corresponding rise in the intermediate SeP. Notably, the fusion expression of SenB and SenC exhibited the highest conversion rate of organic selenium, achieving over 95% under optimal conditions. Our findings provide a basis for organic selenium production through microbial biotechnology. Graphical Selenium is an essential micronutrient in diverse organisms and crucial to life's health. Here, we demonstrated an enhancement of organic selenium biosynthesis by optimising the expression and activity of the two key enzymes, SenB and SenC, through whole-cell catalysis.
研究领域:
微生物学
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生物技术
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营养学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
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2021
·Vol. 11
·期 1
·1-11
·
IF 4.38
Yang Zuoyi,Mao Xingchen,Cui Jiahao,Wang Yujie,Zhang Yaping
This study examines the specific effect of Tween 80 on the conversion of anthracene (ANT) in laccase medium system regarding surfactant chemical changes and mechanism. The conversion rate and degradation products of ANT were investigated in different concentrations of Tween 80 solution. Between Tween 80 concentration 0–40 critical micelle concentrations (CMC), the kinetic parameter-k (h −1 ) and corresponding half-life T 1/2 decreased with increasing concentration. When Tween 80 was above 20 CMC the laccase-medium system converted > 95% of ANT to anthraquinone within 12 h. During the entire enzymatic reaction, the laccase activity in the system increased with increasing Tween 80 concentration. Combined with GC/MS analysis of the product, it was speculated that hydrogens belonging to the ether-oxygen bond and carbon–carbon double bond α-CH of Tween 80, were removed by the laccase-media system, promoting its degradation. Additionally, enhanced activity caused by oxygen free radicals (ROS) such as RO• and ROO•, continuously oxidized Tween 80, which in turn produced free radicals while converting ANT. This study provides new theoretical support toward the application of surfactants in the elimination of polycyclic aromatic hydrocarbons.
研究领域:
环境科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Yanqing Cong,Yudi Wang,Dongchen Lv,Jiayi Gao,Kuangda Chen,Sainan Sun,Xiaoran Wang,Shi-Wen Lv
With more and more attention to health, developing a simple and effective method for detecting phenolic compounds is an urgent and challenging task. In this context, Mn-doped CoP (referred to as Mn-CoP) with a nanocage interior architecture has been successfully constructed. The formation of a hollow structure exposes more active sites, while the incorporation of Mn ion reduces the charge transfer resistance and accelerates the charge transfer. Consequently, Mn-CoP effectively provides electrons to activate H 2 O 2 and O 2 producing OH • and O 2 • , demonstrating excellent peroxidase-like and oxidase-like activities. Given its strong oxidase-like activity, Mn-CoP enables a straightforward colorimetric assay for detecting hydroquinone using 3,3′,5,5′-tetramethyl benzidine (TMB) as colorimetric substrate. Interestingly, the developed colorimetric detection method exhibits a broad linear range and a low limit of detection (LOD) for hydroquinone, showing good potential for application even in real samples. Detailed analyses reveal that the doped Mn ions serve as Lewis acid sites directly reacting with the amino functional group in TMB. The Lewis acid-base reaction between Mn-CoP and TMB is crucial for the chromogenic reaction of TMB. Moreover, doping Mn ion to enhance the oxidase-like activity of the nanozyme is a simple and feasible modification strategy with universal applicability. This work provides a valuable reference for designing nanozymes with oxidase-like activity.
研究领域:
生物化学
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分析化学
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纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Hanfei Ye,Jinqi Shen,Shulin Deng,Wenwen Yu
This study investigates the inhibitory effects of lignin on gluten digestibility and elucidates the underlying mechanism using static in vitro digestion protocols involving pepsin and pancreatin. Gluten digestibility was evaluated based on the degree of protein hydrolysis (DH), peptides’ profile, and free amino acids (AAs) content. The interactions between lignin and gluten under digestive conditions and their impact on proteolytic enzyme activities were examined through various analytical techniques, including scanning electron microscopy (SEM), viscosity measurements, ζ-potential analysis, and enzyme kinetics. Results reveal that lignin significantly impairs gluten digestibility by inducing the formation of protein aggregates, leading to distinct intermediate peptides and reduced levels of key AAs such as aspartic acid, phenylalanine, proline, and histidine. These findings offer valuable insights into gluten-lignin interactions and highlight lignin’s potential applications in the food industry, particularly as a functional additive to modulate nutrient digestibility.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENZYME AND MICROBIAL TECHNOLOGY
·
2017
·
IF 2.502
Shuguang Wang,Debing Zheng,Lingyu Yin,Fei Wang
Cross-linked enzyme aggregates (CLEAs) have emerged as an interesting biocatalyst design for enzyme immobilization. However, the commercialization of CLEAs is often hampered by their shortcomings, such as poor-controlled particle size, low activity and sticky characteristic. In order to overcome these drawbacks, five nanoparticles (NPs) (nano-TiO 2 , nano-MgO, nano-Ni, nano-Cu and nano-Fe 3 O 4 ) were used to improve CLEAs activity and structure in this study. Results showed that moderate dosage of nano-TiO 2 addition increased CLEAs activity, and the most increment was 15.2% relative to CLEAs without NPs. This was possibly due to more channels and smaller size particle of CLEAs after nano-TiO 2 addition. Moreover, nano-TiO 2 addition not only decreased fluorescence intensity but also caused the red shift in tryptophan (Trp) and tyrosine (Typ) residues. Nano-TiO 2 addition decreased K m and increased V max of CLEAs. These changes were benefit to the activity and structure of CLEAs. However, addition of other four NPs (nano-MgO, nano-Ni, nano-Cu and nano-Fe 3 O 4 ) did not increase CLEAs activity and even decrease CLEAs activity due to less amorphous cavities and larger or discrete particle size than CLEAs without NPs. In addition, FT-IR results showed that NPs addition increases the content of regular structure of CLEAs, and causes a partial transformation of β-turn into β-sheet. This study showed that it was potential to improve CLEAs performance by nano-TiO 2 addition.
研究领域:
生物化学
·
酶工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2016
·
IF 4.917
Danlian Huang,Xingmeng Qin,Piao Xu,Guangming Zeng,Zhiwei Peng,Rongzhong Wang,Jia Wan,Xiaomin Gong,Wenjing Xue
A composting study was performed to investigate the degradation of 4-nonylphenol (4-NP) in river sediment by inoculating Phanerochaete chrysosporium ( Pc ). Pc was inoculated into composting Reactor A, C and D, while Reactor B without inocula was used as control. The results showed that composting with Pc accelerated the degradation of 4-NP, increased the catalase and polyphenol oxidase enzyme activities in contaminated sediment. The dissipation half-life (t 1/2 ) of 4-NP in Reactor A, C and D with inocula of Pc were 2.079, 2.558, 2.424 days, while in Reactor B without inocula of Pc it was 3.239 days, respectively. Correlation analysis showed that the contents of 4-NP in sediment in Reactor A and D were negatively correlated with the actives of laccase, whereas no obvious correlation was observed in Reactor B and C. All these findings also indicated that Pc enhanced the maturity of compost, and the best composting C/N ratio was 25.46:1.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
·
IF 6.1
Wenjing Liu,Xiaoxiang Hu,Linghao Fang,Yujie Cai
Spermidine is a naturally occurring polyamine with multiple biological activities and potential food and agricultural applications. However, sustainable and scalable spermidine production has not yet been attained. In this study, a homospermidine synthase (HSS) from Pseudomonas frederiksbergensis (PfHSS) capable of catalyzing the synthesis of spermidine from 1,3-diaminopropane and putrescine was identified based on multiple sequence alignment using Blastochloris viridis HSS (BvHSS) as a template. The optimal reaction pH and temperature for purified PfHSS were determined to be 8.5 and 45 °C, respectively, and K+ was able to promote the enzyme activity. Further analysis of the structural and functional relationships through molecular docking and molecular dynamics simulation indicates that glutamic acid at position 359 is the essential residue for the enzyme-catalyzed synthesis of spermidine. The whole-cell catalytic reaction yielded 1321.4 mg/L spermidine and 678.2 mg/L of homospermidine. This study presents a novel, promising, and sustainable biological method for producing spermidine.
研究领域:
生物化学
·
合成生物学
·
食品科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）