BIOPROCESS AND BIOSYSTEMS ENGINEERING
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2018
·Vol. 41
·期 7
·1051-1060
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IF 2.139
Han Juan,Rong Junhui,Wang Yun,Liu Qian,Tang Xu,Li Cheng,Ni Liang
Abstract Magnetic double-shell hybrid microspheres (Fe 3 O 4 @SiO 2 @p(NIPAM- co -GMA)) have been developed as a promising supported substrate for the immobilization of cellulase. Since the surface of the magnetic microspheres not only contains an epoxy group from GMA (glycidyl methacrylate) that can covalently bind to the enzyme, but also has an intelligent temperature response property from NIPAM ( N -isopropylacrylamide), the cellulase can be covalently bonded to the magnetic microspheres and have a temperature-sensitive capability. The immobilized cellulase has the recovery ability of cellulase activity after a high-temperature inactivation. The average amount and activity of immobilized enzymes, respectively, was 233 mg g −1 , 57.4 U mg −1 under the optimized conditions. The experimental results show that the immobilized cellulase has a wider catalytic temperature range, better temperature and storage stability. The residual activity still remained about 65.6% of the initial activity after the sixth catalysis run, which indicated that the immobilized enzyme had high reproducibility. Graphical abstract
研究领域:
生物化学
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生物技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Jianlin Peng,Yan Zhang,Xiaohong Yang,Meiling Qi
Efficient separation of xylenes, phenols and anilines is a big issue in chemical and petroleum industries. This work presents the first example of employing poly (caprolactone) diol (PCL-Diol) as stationary phase for high-resolution gas chromatographic (GC) separations of these tough isomer mixtures. It showed medium polarity and stronger H-bonding basicity than H-bonding acidity. Impressively, PCL-Diol column exhibited extremely high resolving capability for the isomer mixtures of xylenes, cresols/xylenols, and toluidines/xylidines with good peak shapes. Moreover, it exhibited preferential retention for analytes of a linear alkyl chain, suggesting its shape fitting selectivity for specific analytes. In addition, its separation performance has good repeatability with RSD values on retention times below 0.01% for run to run ( n = 6), 0.67–0.80% for day to day ( n = 4) and 3.2–4.4% for column to column ( n = 4) repeatability, respectively. Furthermore, it was applied for the determination of isomer impurities in real samples, showing good potential for practical use. This work demonstrates the advantageous high-resolution separation performance for challenging isomers and shows its promising future of PCL-Diol-based materials in separation science.
研究领域:
分析化学
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分离科学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2021
·Vol. 13
·期 19
·3270
·
IF 4.329
Sihan Zhang,Zhihua Xu,Jiaqi Guo,Haiying Wang,Yibo Ma,Xianming Kong,Hongtao Fan,Qian Yu
Polystyrene (PS) spheres were prepared through an emulsifier-free emulsion polymerization method, in which the reaction time, ionic strength, concentrations of copolymer were studied in detail. The resulting PS microspheres and Ag nanoparticles were used to construct a surface enhanced Raman scattering (SERS) substrate by a layer-by-layer assembly method. A relatively uniform distribution of PS/Ag in the films was obtained, and the multilayer substrate presented excellent SERS reproducibility and a tunable enhancement effect. The SERS substrate was used for detecting harmful pesticides (malachite green and dimetridazole) in food samples, with a limit of detection as low as 3.5 ppb. The obtained plasmonic composite has a promising future in the field of SERS sensing.Keywords:polystyrene;self-assembly;Ag nanoparticle;layer by layer;SERS;reproducibility
研究领域:
材料科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2025
·
IF 5.1
Tong Song,Jie Liang,Xiling Shi,Zhidong Zhang
Flexible pressure sensors are widely applied in tactile sensing, gait testing, and wearable devices due to their flexibility and repeatability. However, measuring weaker signals needs ultrahigh sensitivity and an extensive detection range. Herein, we propose a novel ionic–electronic sensor featuring a cavity structure made up of Ag electrodes, a PDMS space layer, and an ionic gel. The latter was created by combining polyvinyl alcohol (PVA), chitosan (CS), and 1-ethyl-3-methylimidazole chloride (ionic liquid) after three freeze–thaw cycles. This sensor demonstrated remarkable maximum sensitivity of up to 48 584.56 kPa−1 within the range of 1–10 kPa, a broad pressure range of 0.1–200 kPa, and excellent repeatability over 1000 loading–unloading cycles. Owing to a capacitive effect, a sensor and array sensor were used to detect pulse signals and space pressure distribution, respectively. The signals of each channel of the array sensor were collected through a data-acquisition system. The main peak of the pulse wave was represented by a histogram, and a three-dimensional (3D) pulse wave shape was reconstructed. The 3D pulse wave provides the possibility for the extraction of waveform characteristics. In conclusion, our sensor could be used in portable wearable and flexible smart electronics.
研究领域:
生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
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2019
·Vol. 29
·期 3
·037001
·
IF 2.141
Chunyang Wei,Chengzhuang Yu,Jie Jayne Wu,Junwei Li,Shanshan Li,Shijie Dai,Tiejun Li
Tapered glass capillaries play an important role in microdroplet generation and related microfluidic applications such as particle synthesis, digital PCR and microrectors. However, its controlled production poses a challenge. Specifically, there is difficulty controlling the tip sizes of capillaries with good repeatability at an affordable cost, which is critical to produce standard droplets for use by research groups. To produce tapered capillaries, micro needle geometries are first pulled by a commercial pipette puller, which yields a sharp tip (i.e. 1–3 m). Those tips need to be enlarged and standardized by post microfabrication. Although larger tips could be achieved by advanced pulling-forging, sanding-forging or breaking-forging methods, the uniformity and repeatability do not meet the requirements for mass production. In this paper, several easy-to-operate fabrication approaches, including mechanical and chemical fabrication methods, are studied. Chemical etching fabrication method shows good repeatability and robust control of dimensions. A capillary droplet microfluidic device assembled by tapered glass capillaries fabricated by chemical etching method is also presented, which shows good performance for droplet generations. This non-labor intensive fabrication is straightforward and be readily adopted for industrial scale mass production.
研究领域:
微流控技术
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生物工程
✓ 已验证
DOI
高影响因子
Solar RRL
·
2018
·Vol. 3
·期 4
·1800313
·
IF 0
Xinding Lv,Xuemei Dong,Zhili Ye,Junshuai Zhou,Fei Deng,Yan-Zhen Zheng,Xia Tao
State-of-the art perovskite solar cells (PSCs) are obtained by using a high-crystalline and uniform morphological perovskite film that usually requires a thermal procedure to induce its crystallization. Room temperature processing photoactive perovskite film represents a feasible approach to break through the technology barrier arising from high temperature annealing procedures; however, the photovoltaic performance of fabricated PSCs varies significantly in most routine laboratories with ambient temperature altering. Herein, the authors report for the first time that highly photoactive black phase FA-based perovskite films with high performance and reproducibility can be room temperature processed via the Br induced crystallization effect. It is found that Br adding content varies as a function of ambient temperature fluctuation from 35–40 to 20–25 °C. The halide Br/I formulation are 0.2/2.8 and 0.5/2.5 for room-temperature perovskite crystallization in ambient temperatures of 35–40 and 20-25 °C, yieldings solar to electric power conversion efficiency of 19.59 and 17.53% with high reproducibility, respectively. This efficiency (19.59%) is comparable to the best-performing PSCs based on thermal processing perovskite films. This work provides a valuable and practical guide to room-temperature fabrication of PSCs with high efficiency and reproducibility by delicate control of halide anions.
研究领域:
材料科学
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太阳能技术
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产品关联
DOI
NANOTECHNOLOGY
·
2019
·Vol. 30
·期 47
·475202
·
IF 3.399
Tingting Ge,Sheng Yan,Lingjun Zhang,Hong He,Li Wang,Shunbo Li,Yuan Yuan,Guo Chen,Yingzhou Huang
Surface enhanced Raman spectroscopy (SERS) detection in microfluidics is an interesting topic for its high sensitivity, miniaturization and online detection. In this work, a SERS detection in microfluidics with the help of the Ag nanowire aggregating based on dielectrophoresis (DEP) is reported. The Raman intensities of molecule in microfluidics is greatly enhanced in the naturally generated nanogaps of Ag nanowire aggregating modulated by DEP. Firstly, the influence of DEP voltage and time on Ag nanowire aggregating is investigated to figure out the optimal condition for SERS. And then, the SERS intensities of methylene blue and rhodamine6G at various concentration with high reproducibility and uniformity are studied. Furthermore, the experiment data demonstrate this DEP–SERS system could be repeated used for different molecule detections. At last, the SERS of melamine is measured to explore its application on food safety. Our work anticipates this nanowire assisted repeatable DEP–SERS detection in microfluidics with high sensitivity could meet the emerging needs in environmental pollution monitoring, food safety evaluation, and so on.
研究领域:
分析化学
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纳米技术
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食品安全
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2021
·
IF 9.078
Qian Zhang,Liang Shen,Ping Liu,Panpan Xia,Junliang Li,Han Feng,Caixia Liu,Kun Xing,Aiguo Song,Man Li,Xiaoming Yang,Ying Huang
Blood pressure is an important parameter of a heart-pumping function, vascular peripheral resistance, and blood volume in human body. The non-invasive blood pressure measurement method has attracted tremendous attention due to its advantages of simple operation and comfort. In this paper, graft-modified MWCNTs and water-based polyurethane are used as filler and matrix, which are combined with simple dipping process to produce a resistance-type flexible pressure sensor with crack structure. The sensor with crack structure exhibits a high gauge factor (∼1582.7), fast response time (∼58 ms) and good repeatability (>2000 cycles) under 0–5% strain. By attaching the sensor to the radial artery of human wrist, the pulse waveform is accurately captured. By adopting the time-domain analysis method, in addition to the fast Fourier transform and threshold method which is employed to position and extract the characteristic value of pulse signal, a three-layer back propagation neural network is constructed to regress blood pressure value. The correlation coefficients of systolic blood pressure, diastolic blood pressure and mean blood pressure were 0.950, 0.875 and 0.947, respectively, which could meet the A-level standards of British Hypertension Society and Association for the Advancement of Medical Instrumentation. This study provides a good real-time and portable technology for home blood pressure measurement.
研究领域:
医学工程
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2018
·
IF 3.794
Dan Liu,Peng Shi,Wei Ren,Yantao Liu,Ming Liu,Yijun Zhang,Qijing Lin,Bian Tian,Zhuangde Jiang,Zuo-Guang Ye
La 1-x Sr x CrO 3 -In 2 O 3 thick film thermocouples were fabricated by screen-printing method for high temperature sensing and the effects of Sr 2+ content were investigated systematically. All La 1-x Sr x CrO 3 thick films showed well crystallization with orthorhombic unit cell. Their average particle sizes showed the tendency of first increase then decrease gradually with the increase of Sr 2+ content, and the maximum of average particle size was 1.76 μm. At the same time, the conductivities were improved with the increase of Sr 2+ content. The thermoelectric properties of La 1-x Sr x CrO 3 depended on the Sr 2+ content and the average Seebeck coefficients had an exponential decay tendency with the increase of Sr 2+ content. For La 1-x Sr x CrO 3 (x = 0.1–0.4)-In 2 O 3 thermocouples, excellent repeatability and stability were observed through multi-cycles and long-time usage testing at high temperature. The La 0.7 Sr 0.3 CrO 3 -In 2 O 3 thick film thermocouple with excellent thermal stability (drift rate: 0.81 °C/h) and reliability makes it become a promising candidate high sensitivity thermal sensor .
研究领域:
材料科学
·
传感器技术
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS A-PHYSICAL
·
2023
·
IF 4.6
Ge Gao,Qiang Li,Hongjie Luo,Xiao Huang
Environmentally friendly humidity-sensitive color-changing materials have important applications in humidity indication. Two humidity-sensitive color-changing materials are prepared by utilizing bromocresol purple (BCP)/bromothymol blue (BTB) as humidity color-changing agents, boric acid as chromogenic agent and porous ceramic as substrate. To verify the repeatability and color-changing behavior of humidity-sensitive color-changing materials, a novel quantitative model of humidity is developed using hyperspectral imaging (HSI) and the K-nearest Neighbor (KNN) model. The two samples based on the KNN model can be achieved to match the measured values of ten humidity levels and show good repeatability after 35 cycles. The reflectance of two samples rapidly changed and stabilized within four minutes at a fixed humidity, which reflects their high humidity sensitivity . The predicted values of BCP sample based on KNN model are closer to the measured values of humidity levels in the range of 85–100% RH, while the predicted values of BTB sample based on KNN model are closer to the measured values of humidity levels in 7%− 23% and 85%− 100% RH range . Visualized assessment of humidity levels at each pixel can be obtained to provide a new approach to real-time monitoring of humidity changes , which shows potential applications in many areas.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Caoling Li,Ling Lei,Wenbo Huang,Junxing Hao,Kangbing Wu
The development of simple, low-cost, and reliable analytical methods for detecting Tetrabromobisphenol A (TBBPA) is crucial, given its widespread environmental presence and high biological toxicity. Herein, a ultralow background and renewable electrochemical sensing platform was developed using specific porous copper-organic framework modified carbon paste electrode (Cu-BTC/CPE), enabling highly sensitive and selective detection of TBBPA. Due to its unique porous structure and abundant active sites, the Cu-BTC/CPE exhibits superior electroadsorption capacity (including adsorbed charge and binding energy) as well as enhanced electrocatalytic activity for TBBPA. By leveraging the plasticity and polishability of paraffin oil and graphite powder mixtures, the constructed Cu-BTC/CPE electrode demonstrates admirable repeatability (≥ ten times) and stability (RSD < 5%). Furthermore, the reusable and cost-effective Cu-BTC/CPE-based sensor features a wide linear range of 3–500 nM with a low detection limit of 0.53 nM. It was successfully applied to real samples (plastic e-waste, soil, and sewage), and the results consisted with the values that obtained by high-performance liquid chromatography-mass spectrometry. In brief, this novel sensing system holds considerable promise for rapid, convenient, and on-site monitoring of TBBPA in complex environmental matrices.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Yourong Wu,Wanqian Cai,Jiansen Wen,Shiqi Zhong,Xingan Jiang,Cong Lin,Mei Lin,Tengfei Lin,Min Gao,Chunlin Zhao,Baisheng Sa,Xiao Wu
Powder adsorbents present limited recyclability when used for wastewater treatment. Bulk materials prepared via eco-friendly approaches typically exhibit excellent reproducibility over powders in terms of removing pollutants from aquatic environments. Here, biomass-based aerogels were synthesized via ball milling a mixture of modified moxa ash (a type of moxibustion waste), carbon nanotubes and ammonia, combined with agarose as biomolecule. The obtained aerogels display low density, high porosity, remarkable mechanical properties, and exceptional repeatability and stability for removing organic pollutants. By employing a two-dimensional (2D) aerogel membrane, pollutants such as 10 mg/L mitoxantrone (MTX), 5 mg/L methylene blue, 5 mg/L malachite green, or 5 mg/L rhodamine B could be dynamically adsorbed and swiftly filtered out, achieving nearly 100 % of the removal rate. For three-dimensional (3D) aerogels, efficient removals of MTX (98.4 % at 135 min) and cationic dyes under various conditions were observed, in which the adsorption capacity reached 28.23 mg/g for low concentration of MTX at 120 min. After four cycles, the aerogel still retained 93 % of its original capacity and the removal rate was still 92.4 %. The adsorption process obeys second-order kinetics and adheres to the Freundlich and Temkin isotherm models. Furthermore, the primary mechanism by which the aerogels adsorb MTX includes pore filling, electrostatic interactions, π-π conjugation and hydrogen bonding. The adsorption mechanism was better explained by the results of density functional theory. Biomass-based 2D/3D aerogels are promising, low-cost, eco-friendly and highly efficient absorbents for mitigating wastewater contamination.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）