ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Lu Yang,Nan Wu,Rongrong Bai,Minjiao Chen,Weiliang Dong,Jie Zhou,Min Jiang
We report a facile strategy for the detection of pyruvate in fermentation progress based on a non-enzymatic decarboxylation reaction of pyruvate with hydrogen peroxide (H 2 O 2 ), coupled with a horseradish peroxidase (HRP)-incorporating nanomaterial as an enzyme element to replace conventional pyruvate oxidase (POX). HRP-Cu 3 (PO 4 ) 2 •3H 2 O nanoflowers (HRP-CuP NFs) and thiol graphene (tG) were immobilized onto an Au disk electrode via layer-by-layer assembly to construct an improved amperometric H 2 O 2 biosensor. The as-prepared HRP-CuP NF/tG/Au electrode exhibited a high electrocatalytic performance toward H 2 O 2 , showing a sensitive response of 67.6 μA mM -1 cm -2 , as well as a linear range from 0.1-8.2 mM with a detection limit (LOD) of 0.06 mM (S/N=3). Furthermore, the resulting biosensor also demonstrated a linear range for pyruvate detection from 1.0 to 8.0 mM with LOD of 0.3 mM (S/N=3), under a working potential of -0.3 V. Finally, the biosensor was successfully used for the determination of pyruvate in real microbial fermentation processes without pretreatment, showing an excellent correlation coefficient compared to the standard HPLC assay with RSD values of 3.03-6.45%. This work provides a new approach for the design and preparation of portable sensors for indirect pyruvate detection with high performance, low cost, and easy operation.
研究领域:
生物化学
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传感器技术
·
发酵工程
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DOI
高影响因子
ANALYST
·
2011
·Vol. 137
·期 3
·588-594
·
IF 3.913
Xiaoquan Lu,Dong liu,Jie Du,Haifeng Wang,Zhonghua Xue,Xiuhui Liu,Xibin Zhou
The novel phenomenon of cathodic electrochemiluminescence on a gold electrode in tris(bipyridine) ruthenium(II) (Ru(bpy)32+) solution is described for the first time. A cathodic electrochemiluminescence (ECL) was found to mainly occur at 0.4–0.8 V with continuous potential scanning from 0.2–1.4 V and the ECL peak was observed around 0.68 V, which was quite different from generally reported Ru(bpy)32+ ECL. Our group speculated that Ru(bpy)32+ possibly reacts with the gold electrode in the acidic phosphate buffer solution (PBS) to generate luminescence. The possible ECL mechanism was discussed according to the presented results. Moreover, it is revealed that the Au as co-reactant in the Ru-system contributed dominantly to the whole ECL . Therefore, the reaction between Ru(bpy)32+ and the newly formed Au implied that the inert metal Au could become a promising material for ECL investigations.
研究领域:
分析化学
·
电化学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2018
·Vol. 29
·期 7
·5814-5819
·
IF 2.324
Diao Chunli,Liu Hanxing,Hao Hua,Cao Minghe,Yao Zhonghua
Mn-doped barium strontium titanate Ba 0.4 Sr 0.6 TiO 3 - x mol%Mn ( x = 0, 1, 3 and 5; BSTM x ) thin films were deposited on Pt/Ti/SiO 2 /Si(100) substrates by spin-coating and annealed at 800 °C. X-ray diffraction patterns revealed that all the thin films were a typical cubic perovskite structure and no impurity peaks were observed. The effect of Mn doping on the ferroelectric properties and energy storage performance of the samples was investigated. The results showed that the recoverable energy storage density U rec and the difference ∆ P ( P max − P r ) between maximum polarization P max and remnant polarization P r firstly increased, then decreased with the increase of Mn doping amount. The optimized energy storage performance was obtained in Pt/BSTM3/Pt thin films: energy storage density of 8.48 J/cm 3 and energy storage efficiency of 42.4% at 1.63 MV/cm, electrical resistivity of 4.19 × 10 10 Ω cm and leakage current density of 7.77 × 10 −6 A/cm 2 at 163 kV/cm. Moreover, the effect of different top electrodes on energy storage performance was studied, which displayed that the energy density of Au/BSTM3/Pt films was higher than that of Pt/BSTM3/Pt films. The results indicated that Mn doping and Au electrodes could be effective ways to improve the energy storage properties of BST thin films.
研究领域:
材料科学
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能源存储
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DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jia Du,Xu Peng,Yuting Xu,Li Fu,Di Cao,Qinghua Liu
Odorant substances have been detected in the drinking water of several countries and regions. Although the specific harm of these substances to the human body is not yet clear, they can lead to the decline of drinking-water sensory indicators. In addition, odorants also affect the development of the waterdependent beverage industry and aquaculture industry. Dimethyl isoborneol (2-MIB) is the most common odorant substance that causes soil mildew in drinking water. In this work, for the first time, we first report the electrochemical determination of 2-MIB based on an Au electrode. The electrochemical oxidation of 2-MIB can be achieved due to the excellent conductivity and electrocatalytic property of the Au electrode. The proposed electrochemical sensing method is simple, quick and reliable for 2-MIB determination.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Lihang Zhu,Shuang Li,Wenxin Liu,Jing Chen,Qiwen Yu,Zhen Zhang,Yi Li,Jun Liu,Xing Chen
As an oxidase stress biomarker, 3-nitrotyrosine is closely associated with many cardiovascular diseases. Thus, early diagnosis and real time detection of 3-nitrotyrosine at bedside are highly important. Herein, we developed a handheld electrochemiluminescence (ECL) analysis device, which integrates printed circuit board (PCB) for electrical stimulation and smartphone for optical signals readout. Fast and accurate determination of 3-nitrotyrosine was achieved with Antibody/Ru(dcpy) 3 2+ @AuNPs/MoS 2 modified Au electrode (Ab/Ru@AuNPs/MoS 2 ) for ECL analysis. The linear range of 3-nitrotyrosine detection was from 10 −8 mol/L to 10 −6 mol/L with a detection limit of 8.4 × 10 −9 mol/L. In addition, an Android application was developed to realize real time analysis of ECL emissions and results readout for detection. To confirm the usage of the device, spiked serum with different concentrations was tested and the results indicated the practical reliability and stability of this device. The operating procedure for ECL analysis in this device is extremely easy and electrical stimulation was adjustable from 0 V to 5 V for general ECL systems. Thus, we believe this handheld device for ECL analysis has extensive prospects for application in Point-of-care testing and health caring.
研究领域:
生物医学工程
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分析化学
·
医学诊断
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Li Ding,Xuepeng Wang,Shanxin Yang,Wenda Xu,Yongde Yan,Yun Xue,Fuqiu Ma
In order to reveal the extraction mechanism of Nd on Ga-Al alloy electrode, the electrode reactions and alloy formation between Nd(III), Ga(III) and Al(III) ions in different molten salt systems were studied by multiple electrochemical methods. The cyclic voltammetry curves measured in melts with different concentrations confirmed that only three intermetallic compounds were formed in both Ga-Nd and Al-Nd systems. The preferential interaction of Nd with Ga in Ga-Al-Nd ternary system was further verified by cyclic voltammetry curves with varying potential window. In particular, the co-reduction process of three ions was gradually determined by the comparison of electrochemical behaviour in different melts. In addition, the interfacial reaction of Nd(III) ion on liquid Ga-Al alloy cathode was investigated, and the corresponding exchange current densities were measured through galvanostatic pulse (0.0049, 0.0047, 0.0056 and 0.0055 A cm −2 ) and Tafel method (0.0029 A cm −2 ).
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Hong-Wei Lv,Quan-Fu Li,Hui-Ling Peng
Sodium tetrafluorocuprate (Na 2 CuF 4 ) has been synthesized by the precipitation method. Then, Na 2 CuF 4 was applied to glucose electrocatalysis for the first time, and modified bare Au electrode to obtain Na 2 CuF 4 /Au. Na 2 CuF 4 /Au has strong anti-interference, extremely broad linear range of glucose detection, and great stability through stored for a long time at room temperature. A simple method was used to prepare Na 2 CuF 4 with different morphologies, and the effects of different morphologies for the glucose sensing performance were studied. The optimal experimental parameters of glucose sensing were obtained through parameter optimization, which leads to enhanced glucose sensing performance. The selected Na 2 CuF 4 was composed of large crystals and smaller crystals. The larger crystals sizes of selected Na 2 CuF 4 are in the range from 5 μm to 6 μm, and the smaller are in the range from 230 nm to 300 nm, the two are mixed together. The Na 2 CuF 4 /Au electrode showed high sensitivity and very wide linear range (the first 0.01-0.15 mM, the second 0.15-19.15 mM, the third 19.15-63.15 mM). The Na 2 CuF 4 /Au shows good accuracy, reproducibility and stability, as well as excellent selectivity. The properties of Na 2 CuF 4 indicate that it has a wide application prospect in the field of glucose electrocatalysis as a new glucose sensitive material.
研究领域:
生物医学工程
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分析化学
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产品关联
DOI
高影响因子
Nature Protocols
·
2023
·Vol. 18
·期 8
·2579-2599
·
IF 14.8
Jiang Tao,Yi Long,Liu Xu,Ivanov Aleksandar P.,Edel Joshua B.,Tang Longhua
Studying the electrical properties of individual proteins is a prominent research area in the field of bioelectronics. Electron tunnelling or quantum mechanical tunnelling (QMT) probes can act as powerful tools for investigating the electrical properties of proteins. However, current fabrication methods for these probes often have limited reproducibility, unreliable contact or inadequate binding of proteins onto the electrodes, so better solutions are required. Here, we detail a generalizable and straightforward set of instructions for fabricating simple, nanopipette-based, tunnelling probes, suitable for measuring conductance in single proteins. Our QMT probe is based on a high-aspect-ratio dual-channel nanopipette that integrates a pair of gold tunneling electrodes with a gap of less than 5 nm, fabricated via the pyrolytic deposition of carbon followed by the electrochemical deposition of gold. The gold tunneling electrodes can be functionalized using an extensive library of available surface modifications to achieve single-protein–electrode contact. We use a biotinylated thiol modification, in which a biotin–streptavidin–biotin bridge is used to form the single-protein junction. The resulting protein-coupled QMT probes enable the stable electrical measurement of the same single protein in solution for up to several hours. We also describe the analysis method used to interpret time-dependent single-protein conductance measurements, which can provide essential information for understanding electron transport and exploring protein dynamics. The total time required to complete the protocol is ~33 h and it can be carried out by users trained in less than 24 h.
研究领域:
生物电子学
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纳米技术
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蛋白质科学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2020
·Vol. 167
·期 2
·027519
·
IF 3.721
Jie Yuan,Li Jiang,Xiaopei Tan,Yuhua Yue,Haizhu Shi,Shun Feng
A facile way was developed to fabricate Zr(IV) cation modified gold electrode for high-performance electrochemical sensing of methyl parathion (MP) via layer-by-layer self-assembly. The electrode was prepared by sequentially absorbing 11-mercaptoundecyl-phosphoric acid (MDPA) and Zr(IV) cation on gold electrode through strong chelating interactions between gold and -SH as well as Zr(IV) cation and phosphoric groups of MDPA. Fourier transform infrared spectrophotometry, field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy and cyclic voltammetry were employed for the characterization of morphologies and electrochemical properties. The sensor was used to determine MP by square wave voltammetry. The cathodic peak current (at + 0.25 V vs saturated calomel electrode) increases linearly in the range of 1.0 − 180.0 ng ml−1 under optimized conditions. And the detection limit is low to 0.22 ng ml−1 (based on a signal-to-noise ratio of 3). The electrode is successfully used to detect MP in the real water sample with recoveries of 98.97%–102.4% and RSDs of 0.21%–1.99% (n = 3) at three spiked levels. The proposed method provides one simple way to prepare Zr(IV) cation modified electrode for the fast determination of MP. The reliability and accuracy of the method is validated by independent assays with HPLC.
研究领域:
分析化学
·
环境科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Jianmei Wang,Huihui Zhu,Yuanhong Xu,Wenrong Yang,Ao Liu,Fukai Shan,Mengmei Cao,Jingquan Liu
Herein, a uniform three-dimensional (3-D) graphene nanodots-encaged porous gold electrode was prepared via ion beam sputtering deposition (IBSD) and mild corrosion chemistry for efficient enzyme electrode fabrication. Enzymes, like glucose oxidase and catalase , were modified with pyrene functionalities and then loaded into the graphene nanodots encaged porous gold electrode via non-covalent π–π stacking interaction between pyrene and graphene. The fabricated enzyme electrodes showed profound reusability and repeatability, high sensitivity, inherent selectivity and enhanced detection range. As for glucose analysis a broad linear range from 0.05 to 100 mM was obtained and the linear range for hydrogen peroxide was 0.005 to 4 mM. Detection limits of 30 μM for glucose and 1 μM for hydrogen peroxide were achieved ( S / N = 3), respectively. These electrodes can be applied to analyze the clinical samples with reliable results. The formation mechanism and 3-D structure of the porous electrode were investigated using high resolution transmission electron microscope (HRTEM), atomic force microscopy (AFM), scanning electron microscope (SEM) and electrochemical impedance spectroscopy (EIS). Most importantly, various other ideal biosensors can be fabricated using the same porous electrode and the same enzyme modification methodology.
研究领域:
生物传感器
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材料科学
·
分析化学
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产品关联
DOI
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2015
·
IF 5.337
Ziyao Jiang,Xiaohong Chen,Xuanhuai Lin,Xiangkun Jia,Jinfeng Wang,Likun Pan,Sumei Huang,Furong Zhu,Zhuo Sun
The progresses made in emerging perovskite solar cells, a promising alternative photovoltaic technology to the conventional solar cells , have quickly set the power conversion efficiency (PCE) record of 20%. Apart from the high PCE, the stability of perovskite solar cells is another important issue for them to be commercially viable. To investigate the impact of electrodes on the stability of the perovskite solar cells, cells with a structure of ITO/PEDOT:PSS/CH 3 NH 3 PbI 3 /PCBM/Cathode, having different cathode contacts of Al, Ag and AgAl alloy, were fabricated. The cells with an AgAl alloy cathode reached a PCE of 11.76%, which is slightly higher than that (11.45%) of the structurally identical cells with Ag contact, much higher than that for the ones with Al electrode (7.95%). The stable open-circuit voltage ( V OC ) of cells having an AgAl contact was demonstrated, with almost no change in the V OC after 360 h aging under a relative humidity of 10% in air. However, there is an obvious drop in the V OC of the structurally identical perovskite cells with Ag cathode, e.g., an 85% decrease from its initial V OC value for cells aged under the same condition. The enhancement in the PCE of cells with AgAl cathode is attributed to the formation of AlO X , which can improve built-in potential in the cell and allow an effective electron extraction at the PCBM/AgAl cathode interface. An interfacial AlO X interlayer could be formed at the interface between PCBM and AgAl contact during thermal evaporation and aging. The presence of the interfacial AlO X interlayer helps to prevent the diffusion of the Ag atoms into the active layer, to improve the adhesion of the metal contact on PCBM and also to avoid moisture encroachment.
研究领域:
材料科学
·
光伏技术
·
能源研究
✓ 已验证
产品关联
DOI
高影响因子
Metals
·
2024
·Vol. 14
·期 10
·1142
·
IF 2.6
Jiabao Li,Yang Cai,Jianling Huang,Shiqian Zhao,Deliang Cheng
Mg-based alloy anodes suffer from severe corrosion in alkaline electrolytes, which substantially impedes their cycle life and thereby limits their suitability as anode materials for nickel–metal hydride (Ni-MH) batteries. This work modifies the conventional 6 M KOH electrolyte by adding 0.1 M Al2(SO4)3·18H2O. The electrochemical hydrogen storage properties of Mg0.45Ti0.05Ni0.50alloy in this electrolyte and its microstructural evolution during cycling are studied. In the 6 M KOH + 0.1 M Al2(SO4)3·18H2O electrolyte, a protective layer consisting of Mg2Al(OH)7is formed on the surface of the Mg0.45Ti0.05Ni0.50alloy anode during charge/discharge cycling instead of Mg(OH)2, effectively preventing further corrosion and improving its cycle life. The Mg0.45Ti0.05Ni0.50alloy anode delivers a maximum discharge capacity of 479.0 mAh g−1and maintains 318.4 mAh g−1after 30 cycles in the 6 M KOH + 0.1 M Al2(SO4)3·18H2O electrolyte, which is significantly superior to that achieved in the 6 M KOH electrolyte (471.1 mAh g−1and 201.8 mAh g−1, respectively). This work provides a new strategy for improving the cycle stability of Mg-based alloy anodes.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
产品关联
DOI
高影响因子
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