SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Lixiang Zuo,Ran He,Li Wang,Yanli Wei
The fast and accurate detection of residual veterinary antibiotics in food has great significance for the ecological balance and human health. This study presents a room temperature phosphorescence aptasensing system for sulfadimethoxine (SDM) detection with sulfadimethoxine-aptamer (SDM-APT) modified manganese doped ZnS quantum dots (Mn-ZnS QDs) as the signaling and recognition unit. The signal amplification was achieved by petal-like molybdenum disulfide nano sheets (MoS 2 NS). As the selective adsorption of single-stranded DNA (ssDNA) on MoS 2 NS, SDM-APT-Mn-ZnS QDs are assembled on the surface of MoS 2 NS, and lead to the efficient phosphorescence quenching of Mn-ZnS QDs. Once SDM is brought in the sensing system, the specific interaction between SDM-APT and SDM will switch the conformation of SDM-APT from random coil to hairpin structure, resulting in the exfoliation of SDM-APT-Mn-ZnS QDs from MoS 2 NS, along with the phosphorescence recovery. This SDM detection system has a linear range from 2.0 ng/mL to 400 ng/mL with LOD as 0.91 ng/mL (S/N = 3) at the optimal experimental condition. The feasibility, specificity and recovery have been verified from the detection of real samples, interfering experiments and standard recovery experiments. The SDM detection system may also be applied to other residual veterinary drugs with different aptamers.
研究领域:
分析化学
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食品安全
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环境科学
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2014
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IF 2.367
Xiao Wei,Zhiping Zhou,Jiangdong Dai,Tongfan Hao,Hongji Li,Yeqing Xu,Lin Gao,Jianming Pan,Chunxiang Li,Yongsheng Yan
In this paper, a simple procedure for the determination of 2,4,5-trichlorophenol (2,4,5-TCP) is reported. Mn-doped ZnS quantum dots (QDs) capped by molecularly imprinted polymers (MIPs) were prepared. MIPs were characterized by spectrofluorometer, UV–vis spectrophotometer , X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM) and scanning electron microscope (SEM). Meanwhile, spectrofluorometer was used to the evaluation of optical stability, the effect of pH, and the selective and sensitive determination of 2,4,5-TCP. Under optical conditions, MIPs-capped Mn-doped ZnS QDs were successfully applied to the detection of 2,4,5-TCP in water selectively and sensitively, and a linear relationship was obtained to cover the concentration range of 5.0–50 μmol L −1 with a correlation coefficient of 0.9913. Moreover, 2,4,5-TCP could quench the room temperature phosphorescence of MIPs-capped Mn-doped ZnS QDs in a concentration-dependent manner, which was best described by a Stern–Volmer-type equation.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2017
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IF 4.529
Wengang Zhang,Yong Han,Xiumei Chen,Xueli Luo,Jianlong Wang,Tianli Yue,Zhonghong Li
A Mn-doped ZnS quantum dots (QDs) based nanosensor for selective phosphorescent determination of patulin (PAT) was synthesized with 6-hydroxynicotinic acid (6-HNA) as dummy template via a surface molecular imprinting sol–gel process. FTIR and XRD indicated the successful graft of molecularly imprinted polymer (MIP) onto crystal QDs. Binding tests revealed that the MIP-QDs presented higher selectivity, adsorption capacity and mass transfer rate than non-imprinted polymers, demonstrating a specific recognition for PAT among competitive mycotoxins and its analogues with the imprinting factor of 2.02. The MIP-QDs could recognize PAT in a linear range of 0.43–6.50 μmol L −1 with a detection limit of 0.32 μmol L −1 and a correlation coefficient (R 2 ) of 0.9945. Recoveries of 102.9–127.2% with relative standard deviations <4.95% were achieved in apple juice samples which were in good agreement with high-performance liquid chromatography (HPLC) (P > 0.05). The results indicated a simple phosphorescent nanosensor for PAT detection in complex matrix.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 16
·4618-4623
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IF 3.969
Miao Shao,He-Fang Wang
We presented a homogeneous heparin-mediated fluorescence anisotropy (FA) assay of antithrombin (AT) based on long-lived luminescent polyethyleneimine capped Mn-doped ZnS (PEI-Mn-ZnS) QDs. The PEI-Mn-ZnS QDs with long lifetime luminescence at 585 nm displayed a very low background of FA value, which was very helpful for FA assaying of large molecules. The medium heparin was crucial for AT determination, and different heparin amounts resulted in different linear range of detection and sensitivity. For example, the limit of detection (LOD) of 0.9 nM AT with a detection linear range from 8.6 nM to 21.5 nM was found when the heparin concentration was 75 μM. The proposed method also exhibited high selectivity over the coexisting or related proteins such as human serum albumin and thrombin.
研究领域:
分析化学
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生物医学工程
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纳米技术
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2017
·
IF 0.795
Dan-Dan TANG,Jin-Yi ZHANG,Xian-Deng HOU,Peng WU
The key to maximize the sensitivity of inner filter effect (IFE)-based assay is to enlarge the overlap between the absorption spectra of the absorber and the excitation and/or emission bands of the fluorophore. In this work, Mn-doped ZnS QDs is chosen for IFE-based detection of β -glucuronidase (GUS), since the excitation spectrum of QDs is perfectly overlapped with the absorption spectrum of the substrate of GUS, namely 4-nitrophenyl- β-D- glucuronide (PNPG). Since absorption spectrum of the enzymatic product of GUS-PNP are almost not overlapped with the excitation and emission spectra of Mn-ZnS QDs, the phosphorescent turn-on determination of GUS can be successfully proposed and carried out. In addition, the excellent room temperature phosphorescence property of Mn-ZnS QDs can eliminate the fluorescence background from biological organisms, which can be effectively applied to biological sample analysis with good anti-interference ability. Accordingly, a simple turn-on phosphorescent assay of GUS was developed with a linear range of 10–300 U L −1 and a limit of detection of 7 U L −1 , and the proposed system was also successfully applied for actual detection of Escherichia coli .
研究领域:
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 46
·5919-5928
·
IF 2.378
Nasibeh Saeedzadeh Amiri,Mohammad-Reza Milani-Hosseini
A facile and environmental-friendly strategy was used to prepare ratiometric fluorescence sensor-modified mesoporous-structured epitope-imprinted polymer (QDs@SiO2@EMSiO2) for highly sensitive and selective determination of cytochrome c (Cyt c), using Cu-doped ZnS QDs and Mn-doped ZnS QDs as fluorescent dyes. In this sensor, C-terminal and N-terminal nonapeptides were employed as the template molecules, and C18MIMCl was utilized as a template for mesoporous silica. APTES and PTESMIC were utilized as functional monomers and TEOS was applied as the cross-linker. The as-prepared fluorescence sensor displayed great selectivity and sensitivity by merging ratiometric fluorescence methodology with mesoporous silica materials to create one system. The composite was employed for the recognition of Cyt c, which displayed good linearity in the range from 0.4 nM to 200.0 nM with a detection limit as low as 0.15 nM, which was much lower than those of non-mesoporous and single emission composites. The sensor also exhibited outstanding applicability in complex matrices, which enabled its employment for Cyt c tracing in human serum samples without any pretreatment.
研究领域:
分析化学
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材料科学
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生物传感器
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生物技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 39
·3881-3889
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IF 3.532
Jiao Luo,Hongyan Tan,Bin Yang,Donghua Chen,Junjie Fei
In this study, a mesoporous silica fluorescence probe co-doped with manganese-doped zinc sulfide quantum dots (Mn:ZnS QDs) and rhodamine B (RB) and coated with molecularly imprinted polymer (MIP) has been prepared by sol–gel methods. The morphology and structure were characterized in detail by transmission electron microscopy (TEM), Fourier transform-infrared absorption spectroscopy (FT-IR) and ultraviolet-visible absorption spectroscopy (UV-vis). The probe exhibited two characteristic emission peaks at 411 nm and 582 nm, and the synchronous ratiometric fluorescence responses F411/F582 to different concentrations of 4-nitrophenol (4-NP) showed a good linear correlation in the range of 0.01–10 μmol L−1 besides achieving the sensitive detection of 4-NP with a detection limit as low as 3.0 nmol L−1 (3σ). The probe possesses the advantages of selectivity toward the target molecular structure, self-stability in the detection time domain and anti-interference ability, exhibiting excellent potential for application in 4-NP detection in different water environments.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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