ELECTROPHORESIS
·
2021
·
IF 3.535
Yiling Qu,RuiYang Tao,Huan Yu,Qi Yang,Ziwei Wang,Rui Tan,Xiaochun Zhang,Ruocheng Xia,Lei Xiong,Shihan Xi,Jun Wu,Yuzhen Gao,Suhua Zhang,Chengtao Li
This paper describes the development and validation of a novel 31-locus, six-dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended-CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y-Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing.
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高影响因子
WATER RESEARCH
·
2021
·
IF 11.236
Shi-Qi Tian,Jing-Yao Qi,Yun-Peng Wang,Yu-Lei Liu,Lu Wang,Jun Ma
After reaction with permanganate or ferrate, the resulted Mn-loaded and Fe-loaded biochar (MnOx/biochar and FeOx/biochar) exhibited excellent catalytic ozonation activity. O 3 (2.5 mg/L) eliminated 48% of atrazine (ATZ, 5 μM) within 30 min at pH 7.0, while under identical conditions, ozonation efficiency of ATZ increased to 83% and 100% in MnOx/biochar and FeOx/biochar (20 mg/L) heterogeneous catalytic systems, respectively. Radical scavenger experiment and electron paramagnetic resonance (EPR) analysis confirmed that hydroxyl radical (•OH) was the dominant oxidant. Total Lewis acid sites on MnOx/biochar and FeOx/biochar were 3.5 and 4.1 times of that on the raw biochar, which induced enhanced adsorption of O 3 and its subsequent decomposition into •OH. Electron transfer via redox pairs on MnOx/biochar and FeOx/biochar was observed by cyclic voltammetry scans, which also functioned in the improved catalytic capacity. Degradation pathways of ATZ in MnOx/biochar and FeOx/biochar ozonation systems were proposed, with 34.6% and 44.8% of dechlorination effect accomplished within 30 min of reaction, which was improved by 4.1 and 5.3 times compared to pure ozonation. After 12-hour treatment, acute toxicity of ATZ oxidation products was reduced from 38.3% of pure ozonation system to 14.5% and 6.3% of activated ozonation systems with MnOx/biochar and FeOx/biochar, respectively. Mn-loaded biochar and Fe-loaded biochar have great potential for heterogeneous catalytic ozonation of polluted water.
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高影响因子
Journal of Cleaner Production
·
2020
·
IF 7.246
Xiaoyan Li,Kangkang Wang,Mengqian Wang,Wei Zhang,Jiming Yao,Sridhar Komarneni
In order to avoid the intensive burden of sewage treatment in the traditional dyeing process of indigo caused by the heavy usage of sodium dithionite (Na 2 S 2 O 4 ), an indirect electrochemical reduction dyeing method for denim fabrics was carried out. The indirect electrochemical reduction dyeing with different iron-based complexes was explored by cyclic voltammetry. In addition, the stability of the complex solution, the reduction potential of the dye solution, the rate of dye reduction (Re) and ferrous ion conversion (Xe) in the electrochemical process were investigated, which showed that the Fe(Ⅱ)-DGS-Abal B complex containing ferrous sulfate, sodium gluconate and Abal B (a complexing agent with high alkali resistance and electrical conductivity based on triethanolamine) had stronger reducing capacity. Furthermore, orthogonal experiments and gray clustering analysis were utilized to optimize the indirect electrochemical dyeing process for denim fabric. Under the optimized electrochemical reduction dyeing process, the rate of dye reduction could be up to 91.23% and the K/S value of dyed denim fabric could reach 14.75, which is 6.58% higher than that of the traditional dyeing process. Besides, the dyeing depth is nearly invariable within 7 times of cyclic dyeing and the dyeing liquid is more susceptible to biological and chemical degradation . Compared with the traditional reduction dyeing with Na 2 S 2 O 4 at higher temperature, the electrochemical reduction dyeing strategy uses electrical energy to reduce indigo at room temperature, and the reduction medium could be sustainably utilized by the circulation pump after the cyclic electrochemical reduction dyeing is completed, which not only decreases the COD value of dyeing wastewater, but also saves 63.5% of the economic cost. The eco-friendly electrochemical dyeing strategy presented here with obvious economic benefits could significantly contribute to enhance the sustainability of dyeing process for denim production.
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高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-10
·
IF 6.232
Zhen Deshuai,Shi Sisi,Gao Chan,Kang Qing,Xiao Xilin,Grimes Craig A.,Cai Qingyun
Bi, Fe, and Ti ternary co-doped ZrO 2 (BFT-ZrO 2 ) nanocomposites have been prepared by a sol-gel process and used as both adsorbent and matrix for the enrichment and determination of small molecules by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The BFT-ZrO 2 nanocomposites not only can selectively enrich a wide variety of low-mass toxic pollutants but can also be used as an excellent matrix to enhance the laser desorption/ionization efficiency with low background noise and uniform co-crystalline film. Low limits of detection (LODs) (0.1 pg mL −1 for bisphenol A (BPA), 2 pg mL −1 for tetrabromobisphenol A (TBBPA), 0.1 pg mL −1 for alizarin (AZ), 0.001 pg mL −1 for bisphenol S (BPS), 0.01 pg mL −1 for indigo blue (ID), 0.01 pg mL −1 for pentachlorophenol (PCP), 100 pg mL −1 for estradiol (E2), 0.001 pg mL −1 for cetyltrimethylammonium bromide (CTAB), 0.1 pg mL −1 for crystal violet (CV), 1 pg mL −1 for malachite green (MG), 0.01 pg mL −1 for rhodamine B (RhB), and 0.01 pg mL −1 for perfluorooctane sulfonate (PFOS) were achieved. The relative standard deviations (RSDs) of shot-to-shot are 9.4–24% and of sample-to-sample 5.2–17%. The BFT-ZrO 2 matrix was successfully applied to the determination of TBBPA and BPA in tea samples. This method shows a new strategy for determination of toxic compounds in tea.
Graphical abstract
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高影响因子
MOLECULES
·
2020
·Vol. 26
·期 1
·38
·
IF 3.267
Yao Shen,Bo Chen,Han Zuilhof,Teris A. van Beek
Induced phase separation extraction (IPSE) is an efficient sample clean-up technique that can replace liquid-liquid extraction (LLE). The purpose of this study was to miniaturize IPSE by carrying it out in a microfluidic chip. An IPSE chip was designed and evaluated for its ability to separate and purify samples on a microscale. The 5 × 2 cm chip was fed with a solution of polar to non-polar model compounds in acetonitrile-water (1:1). In the 100 µm wide and 40 µm deep microchannels, the sample solution was efficiently separated into two immiscible phases by adding a hydrophobic solvent as inducer. Analytes present in the sample solution each migrated to their own favorable phase upon phase separation. After optimization, extraction and fractionation were easily and efficiently achieved. The behavior of analytes with a pH-dependent partitioning could be influenced by adjusting the pH of the sample solution.Scutellaria baicalensisextract, used in Traditional Chinese Medicine (TCM), was successfully separated in aglycones and glycosides. In this microscale system, the sample and solvent consumption is reduced to microliters, while the time needed for the sample pretreatment is less than one minute. Additionally, the extraction efficiency can reach up to 98.8%, and emulsion formation is avoided.Keywords:fast sample clean-up;IPSE;miniaturization;green analytical chemistry;μLPME;low solvent consumption;microfluidics;on-chip separation;TCM;Scutellaria baicalensis
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高影响因子
FUEL
·
2020
·
IF 5.578
Kang Yang,Songyan Li,Kaiqiang Zhang,Yongwei Wang
Stability of carbon dioxide foam attracts huge attentions while being of great challenge to many industrial practices. In this paper, aqueous CO 2 foam stabilized with synergy of hydrophilic nanoparticles and nonionic surfactants was experimentally investigated at elevated temperatures and extreme salinities. The foam formula consisting of 1.5 wt% T40 and 2.49 mM C 12 E 23 was determined under elevated temperature and salinity conditions. At 80 °C, the foam volume of the C 12 E 23 /T40 foam is 225 mL, and the half-life is 32 min, which is 13.9 times that of the C 12 E 23 foam. At a salinity of 17 × 10 4 mg/L, the foam volume is 185 mL, and the half-life is 71 min, which is 24.4 times that of the C 12 E 23 foam. With increasing salinity and temperature, the interfacial tension rises, and the viscoelastic modulus gradually declines, resulting in a lower foam stability. However, the T40/C 12 E 23 foam has a good temperature and salinity tolerance, which can be used under harsh reservoir conditions. In a heterogeneous microscopic visualization model, water channeling in high-permeability regions inhibits the further increase in oil recovery. Subsequent injection of the C 12 E 23 /T40 foam improves the oil recovery factor up to 86.2%. The C 12 E 23 /T40 foam enhances the oil recovery by increasing the sweep area and flooding efficiency. In a sandpack model, the plugging pressure gradient of the CO 2 foam stabilized by 2.49 mM C 12 E 23 and 1.5 wt% T40 reaches 25.3 MPa/m, which is 12.65 times higher than that of the C 12 E 23 foam. The composite reinforced foam attains good water blocking and profile control effects, thereby increasing the oil recovery factor 20.1% after water flooding. This study of great importance to improve not only the oil recovery efficiency, also anywhere where CO 2 -in brine foam applicable.
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ACS Omega
·
2021
·
IF 3.512
Huifang Yin,Hongping Chen,Meng Yan,Zhikun Li,Rongdi Yang,Yanjiao Li,Yanfang Wang,Jianyi Guan,Huili Mao,Yan Wang,Yuyang Zhang
Blue indigo dye, an important natural colorant, is used for textiles and food additives worldwide, while another red isomer, indirubin, is the major active ingredient of a traditional Chinese medicine named “Danggui Longhui Wan” for treating various diseases including granulocytic leukemia, cancer, and Alzheimer’s disease. In this work, we constructed a new and highly efficient indigoid production system by optimizing a novel terpenoid cyclase, XiaI, from the xiamycin biosynthetic pathway. Through introducing the flavin-reducing enzyme Fre, tryptophan-lysing and -importing enzymes TnaA and TnaB, and H2O2-degrading enzyme KatE and optimizing the fermentation parameters including temperature, the concentration of isopropyl-β-d-thiogalactopyranoside, and feeding of the l-tryptophan precursor, the final maximum productivity of indigoids by the recombinant strain Escherichia coli BL21(DE3) (XiaI-Fre-TnaAB-KatE) was apparently improved to 101.9 mg/L, an approximately 60-fold improvement to that of the starting strain E. coli BL21(DE3) (XiaI) (1.7 mg/L). In addition, when the fermentation system was enlarged to 1 L in the flask (feeding with 5 mM tryptophan and 10 mM 2-hydroxyindole), the indigoid productivity further increased to 276.7 mg/L at 48 h, including an indigo productivity of 26.0 mg/L and an indirubin productivity of 250.7 mg/L, which has been the highest productivity of indirubin so far. This work provided a basis for the commercial production of bio-indigo and the clinical drug indirubin in the future.
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高影响因子
Forensic Sciences Research
·
2019
·Vol. 7
·期 2
·172-180
·
IF 0
Tao Ruiyang,Chen Chong,Sheng Xiang,Xia Ruocheng,Zhang Xiaochun,Zhang Jingyi,Yang Zihao,Zhang Suhua,Li Chengtao
The Investigator 24plex QS Kit (QIAGEN, Hilden, Germany) is a 6-dye fluorescent chemistry short tandem repeat (STR) polymerase chain reaction (PCR) amplification system that simultaneously amplifies 20 of the expanded Combined DNA Index System (CODIS) core STR loci, SE33, DYS391, and the standard sex-determining locus, amelogenin, as well as two special internal performance quality sensor controls (QS1 and QS2), which are included in the primer mix to check the PCR performance. This study was designed to be a pilot evaluation of this STR-PCR kit in a Chinese Han population regarding the PCR conditions, sensitivity, precision, accuracy, repeatability, reproducibility, and concordance; tolerance to PCR inhibitors; applicability to real “forensic-type” samples; species specificity; mixture, balance and stutter analyses, and utility in a population investigation. The exhaustive validation studies demonstrated that the Investigator 24plex QS system is accurate, sensitive and robust for STR genotyping. In addition, these genetic markers in the population data in our study indicated that they can also be useful for forensic identification and paternity testing in the Chinese Han population.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Xiaoming Chen,Tao Wang,Leimiao Lin,Fangjie Wo,Yaqin Liu,Xiao Liang,Hui Ye,Jianmin Wu
Nanostructured semiconductors are one of the most potent candidates for matrix-free laser desorption/ionization mass spectrometric (LDI-MS) analysis of low-molecular-weight molecules. Herein, the enhanced photoinduced electron transfer and LDI on the tip of a vertical silicon nanowire (SiNW) array were investigated. Theoretical simulation and LDI detection of indigo and isatin molecules in negative ion mode revealed that the electric field can be enhanced on the tip end of SiNWs, thereby promoting the energy and electron transfer to the analytes adsorbed on the tip of SiNWs. On the basis of this finding, a tip-contact sampling method coupled with LDI-MS detection was established. In this strategy, the tip of SiNWs can be regarded as microextraction heads for the sampling of molecules when they come in contact with analytes. Impression of skin, tissue, and pericarp on the vertical SiNW array can effectively transfer endogenous metabolites or exogenous substances onto the tip. Upon laser irradiation, the adsorbed molecules on the SiNW tip can be efficiently ionized and detected in negative ion mode because of the tip-enhanced electron transfer and LDI effect. We believe this work may significantly expand the application of LDI-MS in various fields.
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高影响因子
KEY ENGINEERING MATERIALS
·
2018
·
IF 0
Xiao Dong Tan,Wei Xiong,Jin Zhang,Jie Xu,Chang Hai Yi
The method of electrochemical reduction utilizes electrons as agent to achieve the reduction of indigo, vat dyes and pollution emissions, as well as the recycling of electrolyte. In this study, Ca2+-Fe3+-triethanolamine (TEA) redox system was used as the mediator to achieve the indirect electrochemical reduction of indigo. The optimization of parameter was analyzed by reduction efficiency (RE) and current efficiency (CE). The result showed that indigo could be reduced by electrochemical method and the best optimization recipe was 20 g/L NaOH + 5 g/L Fe2(SO4)3 + 30 g/L TEA + 5 g/L Calcium gluconate + 3 g/L indigo in 1 A·dm-2 current density at 50°C. This work suggested that Ca2+-Fe3+-TEA redox system in reduction of indigo was an effective way with higher CE (75.9 %).
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Journal of Pharmaceutical Analysis
·
2013
·
IF 0
Zheng-Jun Cheng,Hong-Mei Zhao,Qian-Yong Xu,Rong Liu
The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins within site I. The thermodynamic parameters indicated that the binding of indigotin–HSA/BSA mainly depended on the hydrophobic interaction. The binding distance of indigotin to HSA/BSA was evaluated. The results by synchronous fluorescence, three-dimensional fluorescence, Fourier Transform Infrared spectroscopy (FT-IR) and circular dichroism (CD) spectra showed that the conformation of proteins altered in the presence of indigotin.
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Analytical Methods
·
2013
·Vol. 5
·期 4
·925-930
·
IF 1.855
Yajing Lei,Shujie Zhang,Lizheng Fang,Muhammad Sajid Hamid Akash,Weixing Shi,Kedan Sun,Yingchun Xu,Shuqing Chen
Tartrazine is commonly used in foodstuffs, its level is controlled by government, however, it is still used in some food products at levels which are not permitted. We aimed to develop a sensitive and simple method to assess the exposure to tartrazine in pregnant women and the general population in China. A competitive enzyme-linked immunosorbent assay (ELISA) was developed based on polyclonal antibodies for tartrazine. Using this assay, we measured the urinary concentrations of tartrazine in the participants. Under optimal experimental conditions, the standard curve was constructed at concentrations of 0.04–1000 ng mL−1 and the limit of detection (LOD) was set at 0.04 ng mL−1. No cross-reactivity of the antibody was observed with seven other artificial dyes based on this assay. Human blank urine was spiked with tartrazine at concentrations of 0.1, 25, 500 ng mL−1 and analyzed by ELISA, the recoveries of tartrazine were from 99.8% to 106.6% and coefficients of variation were from 3.28% to 14.9%. The study comprised of 351 volunteers (age: 19–69 years), of which 30% were male, 16% were nonpregnant women and 54% were pregnant women. The geometric means for tartrazine in three groups were 2.64, 2.44 and 3.21, respectively. No difference was observed in these groups. The values indicated that both the general population and pregnant women are exposed to tartrazine. Sources of exposure, potential health effects and risk assessment require further investigation.
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高影响因子
危险品化学品经营许可证（带存储）