JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Chuanyu Yang,Peitong Yu,Ying Li,Junyang Wang,Xinyue Ma,Ni Liu,Ting Lv,Hongru Zheng,Han Wu,Hongxia Li,Chunyan Sun
Lead contamination has posed a potential threat to the environment and food safety, arousing extensive concern. In this work, we fabricated a novel fluorescent sensing platform based on zeolitic imidazolate framework-8 (ZIF-8) and DNAzyme for monitoring Pb2+ in water and fish samples. ZIF-8 was proposed as a fluorescence quencher with the advantages of simple synthesis, low cost, and high quenching efficiency. The Pb2+-dependent GR5 DNAzyme containing the large ssDNA loop can be adsorbed onto ZIF-8 accompanied by fluorescence quenching. Upon binding with Pb2+, GR5 DNAzyme was activated and cleaved, leading to the release of FAM-labeled 5-base ssDNA, which restored the fluorescence. The “turn-on” assay can detect Pb2+ through the one-pot procedure in the range of 0.01–10.0 nM with a detection limit of 7.1 pM. The platform is promising for on-site monitoring of Pb2+ owing to the excellent performance of high sensitivity, low background, strong anti-interference ability, and simple operation.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 13
·4130
·
IF 4.927
Qiuhua Zhang,Lingyi Huang,Youjia Wu,Liying Huang,Xiaowen Xu,Renyi Lin
CompoundAnoectochilus roxburghii(Wall.) Lindl. (A. roxburghii) oral liquid (CAROL) is a hospital preparation ofA. roxburghiiandGanoderma lucidum(G. lucidum),which have hepatoprotective effects. Eight active components (five nucleosides/nucleobases and three triterpenoid acids) in CAROL,A. roxburghii, andG. lucidumwere simultaneously detected by high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS). The multiple reaction monitoring (MRM) mode was applied for the detection of analytes. These eight compounds were separated well within 12 min and quantified using the internal standard working curve method. The method showed good linearity (R2> 0.9935) and high sensitivity (limit of detection = 0.29 ng/mL). The analyte recovery ranged from 85.07% to 97.50% (relative standard deviation < 3.31%). The content of the target analytes in four batches of CAROL, and the raw materials ofG. lucidumandA. roxburghiifrom the five regions was determined using this method. The contents of guanosine and ganoderic acid A in four batches of oral liquid were high and stabilized and could be recommended as quality markers (Q-marker) for CAROL. Simultaneous qualitative and quantitative analysis of nucleosides and triterpenoid acids in CAROL,A. roxburghii, andG. lucidumby LC–MS/MS based on the MRM model was reported for the first time. The proposed method provides a sensitive, rapid, and reliable approach for the quality control of Chinese medicinal products.Keywords:LC–MS/MS;Anoectochilus roxburghii(Wall.) Lindl. and its compound oral liquid;Ganoderma lucidum;analysis;quality control
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JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Shitao Feng,An Zhang,Fengshou Wu,Xiaogang Luo,Juan Zhang
This study constructed boronic acid grafted Zr-MOF (BA-Zr-MOF) by a simple pre-installation strategy through mixed organic ligands. Typically, BA-Zr-MOF was prepared by one-step hydrothermal method used for enrichment of cis -diol-containing nucleosides through pipette tip solid-phase extraction (PT-SPE) followed by detection of high-performance liquid chromatography. It is worth mentioning that BA is well assembled into MOF and cis -diol-containing compounds can be efficiently and selectively enriched by abundant boronic acid groups. Three groups of different types of compounds were used to evaluate their selectivity and the results showed the excellent selectivity to cis -diol-containing compounds of as-prepared adsorbent. The BA-Zr-MOF adsorbent possesses a high adsorption capacity, which can reach 86.40 mg g −1 for adenosine. Under the optimal extraction condition, a PT-SPE-HPLC method based on BA-Zr-MOF for analysis of nucleosides was established. The linear range of the four nucleosides is 0.01 to 50 µg mL −1 with R 2 ≥ 0.99 and the detection limits (LODs) are estimated at between 0.005-0.012 µg mL −1 . The recoveries in urine were used to test the reliability of the analytical methods, which ranged from 82.8% to 117.1%, with intra-day relative standard deviations (RSDs) ranged from 0.1% to 4.2% and the inter-day RSDs ranged from 0.2% to 6.2%. All the results show that the pre-installation strategy based on dual ligands is an alternative to fabricate MOF composite material and BA-Zr-MOF is a promising material for the analysis of cis -diol-containing biomolecules.
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Food Bioscience
·
2022
·
IF 5.318
Jia-Xin Lin,Tao Xiong,Zhen Peng,Mingyong Xie,Fei Peng
Hyperuricemia has been identified as the second most common metabolic disease, increasing the incidence of gout, hypertension, kidney disease, and cardiovascular disease. Lactic acid bacteria have been attracting considerable interest in anti-hyperuricemia given various probiotic functions. In this study, sixteen lactic acid bacteria were selected from one hundred ninety-two isolates through whole-cell catalysis, affording β-D-glucosidase activity of 0.1652–0.3346 U/mg wet cells, degradation rate of inosine-transformed rate of 1.2125–4.6555 mM/h and guanosine-transformed rate of 0.2904–1.8303 mM/h. The sixteen candidates’ acid tolerance, bile resistance, hydrophobicity , and auto-aggregation were further evaluated. According to the results of the seven indexes, the five top-scored strains were viewed as the potentially functional probiotics for anti-hyperuricemia via the comprehensive evaluation of principal component analysis . Cell assay showed that the adhesion of the five strains to Caco-2 cells ranged from 2.67% to 8.67%. Moreover, the five strains can decrease the adhesion of E. coli to Caco-2 cells by 36.67–98.73% through competition inhibition, exclusion inhibition, and replacement inhibition. Finally, the five probiotics were sensitive to some of the antibiotics examined and were innocuous with nonhemolytic activity. The preliminary works indicate that the five candidates could show a good potential application against hyperuricemia.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuhong Xia,Tian Lu,Liping Wang,Jinling Mo,Yang Jin,Liying Zhang,Shuhu Du
Methylglyoxal (MGO) is the primary material basis for the non-peroxide antibacterial activity (NPA) of manuka honey from New Zealand. Therefore, it is necessary to identify the quality or discriminate the grade of honey because no all manuka honeys on the market display the NPA. The current routine method employed for the detection of MGO involves high-performance liquid chromatography (HPLC) test. However, it requires long time (∼8 h) for sample derivatization . Herein, we report an intrinsic Raman signal amplification strategy for the rapid identification and detection of MGO by using silver-coated gold nanoparticles (Au@Ag NPs) along with a high selective surface-enhanced Raman scattering (SERS) probe 8-thioguanosine (8-TG). 8-TG is synthesized via the derivatization of 8-bromoguanosine (8-BG) with thiourea , and its Raman peak assignments were confirmed by computer simulation. The detection is performed through the Raman intensity ratio (I 631 /I 700 ) variation of N 2 -(1-carboxyethyl)-thioguanosine (CETG) formed by the reaction between 8-TG and MGO on surface of Au@Ag NPs, where one CETG Raman intensity at 631 cm −1 increases while the other one at 700 cm −1 decreases oppositely. The opposite change not only yields an intrinsic Raman signal amplification, but also provides built-in correction. As a result, the proposed SERS method exhibits high sensitivity and accuracy. In addition, the whole analytical test is achieved within ∼20 min. The method can be used for the fast detection of MGO in manuka honey and discrimination of the honey grade.
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BIOSENSORS & BIOELECTRONICS
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2021
·
IF 10.618
Xiang Wang,Xin Yan,Bin-cheng Wang,Wei Xue,Chang-ren Zhou,Yi Zhang,Zong-hua Liu
Blood lead poisoning is a universal and severe health problem that greatly threatens human health in various industries. Elimination of blood lead relying on chelating agents and combination with hemoperfusion adsorbents has achieved considerable progress, but it is still suffering from the compromised selectivity of adsorbents as well as in absence of real time monitoring during treatment. Herein, we proposed a selective blood lead adsorbent integrated with real-time visualized/electrochemical bi-modal monitoring based on TMPyP-bound guanosine-borate (GB) supramolecular hydrogel as potential smart hemoperfusion device. The GB hydrogel possessed stability in physiological environment, self-healing ability resistant to fluid shear, blood compatibility, selective adsorption of lead ions superior to conventional adsorbents, anti-fouling performance to blood components and renewability. Benefiting from binding with TMPyP and the intrinsic conductivity, GB hydrogel was endowed with the ability to qualitatively diagnose the presence of blood lead via simple color change and quantitatively reflect the amount of adsorbed lead from blood accurately through electrochemical technique . This work puts forward an integrated treatment/monitoring hemoperfusion device with high selectivity, simple fabrication and low-cost, providing a paradigm for next generation design of intelligent, monitorable theranostic hemopurification system, which is also an extensible platform for the other research fields such as environmental monitoring and remediation.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 4
·1383-1391
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IF 2.647
Yang Hongmei,Hu Peiyu,Tang Jing,Cheng Ying,Wang Fang,Chen Zilin
A simple and sensitive bifunctional electrochemical aptasensor for detection of adenosine and thrombin has been developed using gold nanoparticles–electrochemically reduced graphene oxide (AuNPs-ERGO) composite film-modified electrode. Firstly, the reduced graphene oxide film and AuNPs were sequentially immobilized on glassy carbon electrode (GCE) surface. Secondly, thrombin aptamer was immobilized on the modified electrode. Finally, adenosine aptamer was hybridized with it to serve as a recognition element and methylene blue (MB) as electrochemical signal indicator. In the presence of adenosine or thrombin, the sensor recognized it and a conformational change was induced in aptamer, resulting in decrease of the peak current of MB. The linear relation between concentration of adenosine or thrombin and peak current of MB allowed quantification of them. Thanks to the special electronic characteristic of AuNPs-ERGO composite film, sensitivity of sensor was greatly improved. Under optimal conditions, the proposed aptasensor presented an excellent performance in a linear range of 25 nM to 750 nM for adenosine and 0.5 nM to 10 nM for thrombin. Detection limits were estimated to be 8.3 nM for adenosine and 0.17 nM for thrombin, respectively. Moreover, dual-analyte detection of adenosine and thrombin was achieved without potentially increasing the complexity and cost of the assay.
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JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Houmei Liu,Pian Jin,Mi Jiang,Yu Duan,Guozheng Zhu,Hui Yu,Hongdeng Qiu
In this work, for the first time five amine-ligands including mono-amine, di-amine, tri-amine, secondary and tertiary amine, were functionalized on mesoporous micro-silicas and developed as stationary phases for hydrophilic interaction liquid chromatography (HILIC). The investigations about the retention mechanisms, effects of different chromatographic conditions and stability were systematically conducted. Three kinds of polar and hydrophilic compounds (saccharides, sulfonamides, nucleosides and nucleobases) were selected as probe molecules to evaluate their separation performances. Among the five stationary phases, only aminopropyl-bonded silica has already gained wide developments and applications. Whereas, there are no related researches about the other four to be utilized as separation media. By a series of chromatographic evaluations, the results revealed the other four mesoporous micro-silica materials functionalized with di-amine, tri-amine, secondary and tertiary amine, had great potential to be explored as novel stationary phases of HILIC. Particularly, the two stationary phases functionalized with di-amine and tri-amine exhibited outstanding separation and retention abilities. This work offered some insights on the understanding of retention in HILIC mode and provided us possibility to explore other amine-based HILIC stationary phases.
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Analytical Methods
·
2020
·Vol. 12
·期 46
·5635-5641
·
IF 2.596
Dan Liu,Lili Meng,Liping Huang,Shipeng Luo,Guohua Zhang
Chitosan is a considerably versatile and promising biomaterial and can easily form a 3D hierarchical porous scaffold. In this work, a novel boronate-affinity monolithic column modified with a boronic acid–chitosan complex was prepared and characterized by different methods such as Fourier transform infrared spectroscopy, scanning electron microscopy, thermal gravimetric analysis, specific surface area analysis and pore size distribution analysis. The synthesized monolithic column was used for polymer monolithic microextraction combined with high performance liquid chromatography for the simultaneous determination of cytidine, uridine, inosine, and guanosine in milk powder samples. Several parameters affecting extraction efficiency, including the eluent proportion, eluent flow rate, sample flow rate, sample volume, and sample pH were investigated. The boronate-affinity monolithic column showed high enrichment ability due to the selective formation of cyclic borate esters between nucleosides and boronic acid groups at high pH and the release of cyclic borates at low pH values. Under the optimum operating conditions, the linear range was 0.1–50 μg mL−1, and the correlation coefficients were in the range of 0.9993–0.9994. The LOD and LOQ were in the range of 0.0027–0.0034 and 0.0090–0.011 μg mL−1, respectively. In addition, the results of recovery and relative standard deviation were satisfactory.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Lianjing Zhao,Dehao Kong,Zepei Wu,Guannan Liu,Yuan Gao,Xu Yan,Fangmeng Liu,Xiaomin Liu,Chenguang Wang,Jiuwei Cui,Geyu Lu
The combination of nanomaterials and DNA has captured extensive attention in the fields of sensor, catalysis, biomedicine and material science. The applications of DNA functionalized MoS 2 nanosheets (NSs) in biosensor and biomedicine in turn present an fascinating topic about biointerface science between DNA and MoS 2 NSs. The surface interaction between DNA and MoS 2 NSs was explored by adsorption and desorption experiments. The results indicated that van der waals force played an crucial role in DNA adsorption by MoS 2 NSs. Additionally, it was proved that the quenching of dye labelled DNA probe by MoS 2 NSs was attributed to fluorescence resonance energy transfer. Based on the extraordinarily high quenching efficiency of MoS 2 NSs and different adsorption affinity of MoS 2 NSs toward aptamer and aptamer-targeted protein, an aptameric biosensor was fabricated to detect carbohydrate antigen 15–3. The fabricated biosensor not only displayed high sensitivity with the detection limit of 0.0039 U/mL (S/N = 3), but also exhibited good stability and selectivity. The proposed sensing platform provided an easy-constructed and cost-effective protocol to detect other aptamer targeted biomolecules. The fundamental study of biointerface science on DNA and MoS 2 NSs will facilitate their potential applications in various fields.
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CATALYSIS LETTERS
·
2020
·Vol. 150
·期 9
·2607-2616
·
IF 2.482
Huang Benhua,Gao Ruixia,Pan Cheng,Fan Chao,Jia Xin,Pan Wanli,Li Xiaoyong,Li Heng,Sun Yang,Zhang Donghua
Abstract The atrolactic acid is an important intermediate for synthesis of non-steroidal anti-inflammatory pharmaceuticals, and meanwhile the traditional synthesis of atrolactic acid demands harsh conditions, lengthy steps, or big power consumption. In order to overcome these drawbacks, a series of Ce–Zr binary oxides are first developed as catalysts for oxidation of α-methylstyrene oxide into atrolactic acid. Catalytic results reveal these catalysts have high activity and recycling potential. This work shows a new method for preparation of atrolactic acid, which could be carried out under benign conditions, providing satisfactory outputs. Graphic Abstract
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-10
·
IF 6.232
Guo Rui,Wang Dan-Ni,Wei Yun-Yun,Zhang Ying-Zhi,Yang Chun-Guang,Xu Zhang-Run
A controllable approach for preparing a portable colloidal photonic crystal (CPC) array chip is presented. The approach was inspired by the confinement effect of nanoparticle self-assembly on patterned surface. Hydrophobic polydimethylsiloxane substrate with reproducible micro-region array was fabricated by soft-lithography. The substrate was employed as the patterned template for self-assembly of monodisperse polystyrene nanoparticles. The CPC units can be prepared in several minutes, and exhibit consistent reflection wavelength. By adjusting the size of polystyrene nanoparticles and the shape of micro-regions, CPC units with multiple structure, colors and geometries were obtained. The CPC array chip features fluorescence enhancement owing to the optical modulation capability of the periodic nanostructure of the self-assembled CPC. With the reflection wavelength (523 nm) of green CPC units overlapping the emission wavelength (520 nm, with excitation wavelength of 490 nm) of 6-carboxyfluorescein-labeled DNA probe, the fluorescence intensity increased more than 10-fold. For signal-amplified assay of adenosine, the concentration range of linear response was 5.0 × 10 −5 mol L −1 to 1.0 × 10 −3 mol L −1 , and the limit of detection was 1.3 × 10 −6 mol L −1 . Because of the enhancement effect of photonic crystal, the fluorescence images were more readable from the CPC array chip, compared with those from the planar substrate. The chip has potential applications in multiplex determination with high-throughput via encoding strategy based on the tunable structure, color or geometric shape.
Schematic diagram of signal-enhanced fluorescent detection of adenosine based on the colloidal photonic crystal array chip (PDMS, polydimethylsiloxane; PS NPs, polystyrene nanoparticles; CPC, colloidal photonic crystal; GO, graphene oxide; FAM, 6-carboxyfluorescein).
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