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Food Chemistry-X · 2025 · IF 8.2

Influence of fermented feed on lipid composition and volatile flavor profile of duck eggs: Insights from multi-omics analysis

Qishan Dong,Lizhi Lu,Tiantian Gu,Yong Tian,Yibo Zong,Jing Sun,Yu Huang,Qiuling Fu,Jun He,Tao Zeng

This study employed a multi-omics approach to examine the impact of feed fermentation on the lipid profile and volatile flavor of duck eggs. Sensory evaluation and GC × GC-TOFMS analyses demonstrated that microbial fermentation in feed significantly reduced the off-odor of duck eggs. Among the thirty-nine differential volatile compounds identified, six—namely 3-methylbutanal, 1-octen-3-ol, hexanal, acetophenone, 2-heptanone, and 2-pentylfuran—were likely responsible for the alteration in yolk aroma. Lipidomics and metabolomics identified twenty-five key differential lipids ( p <  0.05, VIP > 1.85) and a modified metabolic pathway associated with linoleic acid (LA), involving four metabolites. Correlation analysis revealed significant associations between LA-containing lipids (e.g., DG (18: 2/18:2)), LA metabolites, and differential volatiles (e.g., 3-methylbutanal) ( p <  0.05). These results provide insights into the mechanisms underlying off-odor reduction and offer a potential strategy for enhancing the flavor profile of duck eggs.

CAS / MDL: 64-17-5
研究领域: 食品科学 · 代谢组学 · 动物营养学
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FOOD CHEMISTRY · 2025 · IF 9.8

Improving lipidomic coverage for animal matrices by optimizing extraction methods and resuspension solvents based on UHPLC-Q-Exactive Orbitrap MS/MS

Ying Wang,Xinhui Bi,Xuhui Huang,Dongmei Li,Lei Qin,Yuying Zhang

Due to the complexity of animal matrices and the different polarities of lipids, the measurement methods are expected to cover a comprehensive range of lipid species. Three extraction methods (chloroform/methanol, methyl tert -butyl ether, and Soxhlet methods) and three resuspension solvents (methanol, isopropanol/acetonitrile = 1:1, and isopropanol/acetonitrile = 9:1) were used to compare the lipid species, contents, and ionization efficiency of Spanish mackerel and duck muscles. Overall, the chloroform/methanol extraction method was the most effective in capturing a diverse array of lipid species. The redissolution solvent isopropanol/acetonitrile (1,1) was highly efficient for detecting triglycerides, while methanol was effective in detecting diacylglycerols and phospholipids. Optimizing the measurement conditions of lipids with different polarities provides a valuable reference for lipid extraction and structural elucidation in different muscle tissues. Furthermore, it supports the determination of differential markers for animal lipid metabolism studies or food authentication using an untargeted lipidomic approach.

研究领域: 分析化学 · 生物化学
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Nanomaterials · 2023 ·Vol. 13 ·期 18 ·2523 · IF 5.3

Interaction of Cerium Oxide Nanoparticles and Ionic Cerium with Duckweed (Lemna minor L.): Uptake, Distribution, and Phytotoxicity

Yang Liu,Xuepeng Zhao,Yuhui Ma,Wanqin Dai,Zhuda Song,Yun Wang,Jiaqi Shen,Xiao He,Fang Yang,Zhiyong Zhang

As one of the most widely used nanomaterials, CeO2nanoparticles (NPs) might be released into the aquatic environment. In this paper, the interaction of CeO2NPs and Ce3+ions (0~10 mg/L) with duckweed (Lemna minorL.) was investigated. CeO2NPs significantly inhibited the root elongation of duckweed at concentrations higher than 0.1 mg/L, while the inhibition threshold of Ce3+ions was 0.02 mg/L. At high doses, both reduced photosynthetic pigment contents led to cell death and induced stomatal deformation, but the toxicity of Ce3+ions was greater than that of CeO2NPs at the same concentration. According to the in situ distribution of Ce in plant tissues byμ-XRF, the intensity of Ce signal was in the order of root > old frond > new frond, suggesting that roots play a major role in the uptake of Ce. The result of XANES showed that 27.6% of Ce(IV) was reduced to Ce(III) in duckweed treated with CeO2NPs. We speculated that the toxicity of CeO2NPs to duckweed was mainly due to its high sensitivity to the released Ce3+ions. To our knowledge, this is the first study on the toxicity of CeO2NPs to an aquatic higher plant.

研究领域: 植物学 · 环境科学
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Food Packaging and Shelf Life · 2024 · IF 8.5

Edible freshness-keeping film prepared by Pouteria campechiana pericarp residue and applied to the preservation of duck breast meat

Ren Liu,Kejun Li,Liangyue Fang,Wanru Luo,Shuangfei Wang,Chongxing Huang

The global waste rate of fresh meat products is approximately 20 – 30%, with the vast majority occurring in the consumer and retailer sectors. The use of antimicrobial preservative films can reduce fresh retail meat waste. In this study, we propose an environmentally friendly antimicrobial preservative film prepared using the residue of Pouteria campechiana pericarp as a substrate, sodium alginate as the film-forming agent, and nisin as the antimicrobial agent. This film was used to preserve duck breast meat. Nisin in the film slows the spoilage of duck breast meat by inhibiting and killing microorganisms, thereby extending its four-day shelf life. It is important to note that mouse-feeding experiments on the film have proven that it is safe and edible. This strategy provides ideas for the reuse of agricultural waste, and this edible antimicrobial film has great potential for the preservation of fresh meat.

研究领域: 食品科学 · 生物技术 · 环境科学
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ANIMAL SCIENCE JOURNAL · 2017 ·Vol. 88 ·期 11 ·1753-1762 · IF 1.325

Dietary α-ketoglutarate supplementation improves hepatic and intestinal energy status and anti-oxidative capacity of Cherry Valley ducks

Shuangshuang Guo,Rui Duan,Lei Wang,Yongqing Hou,Linglin Tan,Qiang Cheng,Man Liao,Binying Ding

α-Ketoglutarate (AKG) is an extensively used dietary supplement in human and animal nutrition. The aim of the present study was to investigate effects of dietary AKG supplementation on the energy status and anti-oxidative capacity in liver and intestinal mucosa of Cherry Valley ducks. A total of 80 1-day-old ducks were randomly assigned into four groups, in which ducks were fed basal diets supplemented with 0% (control), 0.5%, 1.0% and 1.5% AKG, respectively. Graded doses of AKG supplementation linearly decreased the ratio of adenosine monophosphate (AMP) to adenosine triphosphate (ATP) in the liver, but increased ATP content and adenylate energy charge (AEC) in a quadratic and linear manner, respectively ( P < 0.05). Increasing dietary AKG supplemental levels produced linear positive responses in ATP content and AEC, and negative responses in AMP concentration, the ratio of AMP to ATP and total adenine nucleotide in the ileal mucosa ( P < 0.05). All levels of dietary AKG reduced the production of jejunal hydrogen peroxide and hepatic malondialdehyde ( P < 0.05). Hepatic and ileal messenger RNA expression of AMP kinase α-1 and hypoxia-inducible factor-1α were linearly up-regulated as dietary AKG supplemental levels increased ( P < 0.05). In conclusion, dietary AKG supplementation linearly or quadratically enhanced hepatic and intestinal energy storage and anti-oxidative capacity of Cherry Valley ducks.

CAS / MDL: 17091-15-5
研究领域: 动物营养学 · 生物化学
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INTERNATIONAL JOURNAL OF FOOD PROPERTIES · 2018 · IF 1.845

Effect of bilayer coating composed of polyvinyl alcohol, chitosan, and sodium alginate on salted duck eggs

Yongli Jiang,Chenjun Zhuang,Yu Zhong,Yanyun Zhao,Yun Deng,Haiyan Gao,Hangjun Chen,Honglei Mu

The composite bilayer film based on polyvinyl alcohol, sodium alginate, and chitosan were developed in this study, and the effects of the related coating on internal-quality changes of salted duck eggs (SDEs) during storage were determined. The results showed that the overall properties of the bilayer film were better than those of monolayer films, including storage stability. Coatings could significantly (p < 0.05) reduce quality deterioration of SDEs such as water loss, oil exudation, and gritty. The bilayer coating was more effective in inhibiting the increase of egg yolk pH and total bacterial counts than monolayer coatings. After storage for 60 days, water content of bilayer coated SEDs was 19.7% higher than that of the control SDEs, oil exudation was 38% higher, and gritty texture was 27.9% higher. Moreover, bilayer coating could prolong the shelf life of SDEs to more than 60 days at 25°C and 50% relative humidity according to the results of total bacterial count experiment, while the shelf life of control SDEs was less than 45 days. This study indicated the potential application of bilayer composite coating for preservation of SDEs.

CAS / MDL: 13463-67-7 9002-89-5
研究领域: 食品科学 · 材料科学
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SCIENCE OF THE TOTAL ENVIRONMENT · 2023 · IF 9.8

Molybdenum exposure induces inflammatory response via the regulatory effects of lncRNA-00072124/miR-308/OSMR crosstalk on JAK/STAT axis in duck kidneys

Wenjing Pu,Xuesheng Chu,Shiwen Xu,Xueyan Dai,Li Xiao,Ting Cui,Bingyan Huang,Guoliang Hu,Caiying Zhang

Molybdenum (Mo) is an essential nutrient in living organisms. Although numerous researchers have noticed the health damage caused by excessive Mo, the underlying mechanism of excessive Mo-induced nephrotoxicity remains poorly understood. A gene crosstalk called competitive endogenous RNAs (ceRNAs) can interpret many regulatory mechanisms molecularly. But there are few researches have tried to explain the damage mechanism of excess Mo to organisms through ceRNAs network. To clarify this, the study explored the changes in lncRNAs and miRNAs expression profiles in the kidney of ducks exposed to excess Mo for 16 weeks. The sequencing results showed that Mo exposure caused differential expression of 144 lncRNAs and 14 miRNAs. The occurrence of inflammation through the JAK/STAT axis was observed and the lncRNA-00072124/miR-308/OSMR axis was verified by a double luciferase reporter assay. Overexpression of miR-308 and RNA interference of OSMR reduced Mo-induced inflammatory factors, while miR-308 knockdown showed the opposite effect. Simultaneously, lncRNA-00072124 affected OSMR function as a ceRNA. Taken together, these results concluded that Mo exposure activated the JAK/STAT axis and induced inflammation mediated by the lncRNA-00072124/miR-308/OSMR crosstalk. The results might provide new views for revealing the toxic effects of excess Mo in duck kidneys.

CAS / MDL: 12054-85-2
研究领域: 毒理学 · 分子生物学 · 动物科学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Foods · 2020 ·Vol. 9 ·期 4 ·449 · IF 4.092

Preparation and Characterization of Coating Based on Protein Nanofibers and Polyphenol and Application for Salted Duck Egg Yolks

Qiannan Wang,Weihua Liu,Bo Tian,Dongmei Li,Chunhong Liu,Bin Jiang,Zhibiao Feng

Salted duck egg yolk (SDEY) is one of the traditional pickled egg products in Asian countries, which suffers from the weight loss and deterioration of texture characteristics during storage. To better maintain the texture of SDEY, an edible coating based on whey protein isolate nanofibers (WPNFs) with glycerol (Gly) as a plasticizer and incorporating carvacrol (CA) as an antimicrobial agent was developed. Whey protein isolate (WPI, 5%) was used to self-assemble into WPNFs at 80 °C for 10 h. The particle size, zeta-potential and microstructure of WPNFs–CA emulsion were investigated to evaluate the distribution. Results proved that WPNFs–CA emulsion had smaller particle size and better distribution than WPI–CA emulsion. WPNFs–CA/Gly edible coating was then prepared based on WPNFs–CA emulsion. The WPNFs–CA/Gly edible coating exhibited higher antibacterial activity while the WPNFs–CA/Gly film had smooth and continuous surfaces and better transmittance compared with other samples. Furthermore, weight losses and textural properties changes of SDEYs with WPNFs–CA/Gly coating were evaluated. Results proved that salted duck egg yolks with WPNFs–CA/Gly coating exhibited lower weight losses. Textural properties were significantly improved by the WPNFs–CA/Gly coating on SDEYs than those uncoated samples. It was noted that the egg yolks coated with the WPNFs–CA/Gly coating had the lowest hardness increase rate (18.22%). Hence, WPNF-based coatings may have a good development prospect in the food industry.Keywords:whey protein isolate nanofibrils;edible coating;antibacterial activity;salted duck egg yolks

CAS / MDL: 499-75-2
研究领域: 食品科学 · 生物材料学
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FOOD HYDROCOLLOIDS · 2022 · IF 11.504

Effect of desalted egg white and gelatin mixture system on frozen dough

Bin Zhou,Yalei Dai,Dashan Guo,Jiajia Zhang,Hongshan Liang,Bin Li,Jing Sun,Jian Wu

During freezing, the formation and recrystallization of ice crystals cause damage to the frozen dough. The effect of desalted duck egg white and gelatin (DDG) on the overall changes of frozen dough during storage was studied. The results showed that the addition of DDG slowed down the increase of the freezable water content and water fluidity of dough, increased the elastic and viscous modulus, and reduced the loss tangent (tanδ) during the frozen storage process, thus improving the quality of frozen dough. After 28 days of frozen storage in the presence of DDG, the levels of free thiols in frozen dough increased less than those in the control group, weakening the damage to the secondary structure and microstructure of the protein. The addition of DDG had a positive effect on the quality of frozen dough during the 0–28 days frozen storage.

CAS / MDL: 9000-70-8
研究领域: 食品科学 · 生物化学
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ENVIRONMENTAL TOXICOLOGY · 2022 ·Vol. 37 ·期 11 ·2660-2672 · IF 4.109

Cadmium exposure induces autophagy via PLC-IP3-IP3R signaling pathway in duck renal tubular epithelial cells

Huiling Guo,Bingyan Huang,Ting Cui,Xuesheng Chu,Wenjing Pu,Gang Huang,Chenghong Xing,Caiying Zhang

Cadmium (Cd) is detrimental to animals, but nephrotoxic effects of Cd on duck have not been fully elucidated. To evaluate the impacts of Cd on Ca homeostasis and autophagy via PLC-IP 3 -IP 3 R pathway, primary duck renal tubular epithelial cells were exposed to 2.5 μM and 5.0 μM Cd, and combination of 5.0 μM Cd and 10.0 μM 2-APB or 0.125 μM U-73122 for 12 h (U-73122 pretreated for 1 h). These results evidenced that Cd induced [Ca 2+ ] c overload mainly came from intracellular Ca store. Cd caused [Ca 2+ ] mit and [Ca 2+ ] c overload with [Ca 2+ ] ER decrease, elevated Ca homeostasis related factors (GRP78, GRP94, CRT, CaN, CaMKII, and CaMKKβ) expression, PLC and IP 3 activities and IP 3 R expression, but subcellular Ca 2+ redistribution was reversed by 2-APB. PLC inhibitor U-73122 dramatically relieved the changes of the above indicators induced by Cd. Additionally, U-73122 obviously reduced the number of autophagosomes and LC3 accumulation spots, Atg5, LC3A, LC3B mRNA levels and LC3II/LC3I, Beclin-1 protein levels induced by Cd, and markedly elevated p62 mRNA and protein levels. Overall, the results verified that Cd induced [Ca 2+ ] c overload mainly originated from ER Ca 2+ release mediated by PLC-IP 3 -IP 3 R pathway, then triggered autophagy in duck renal tubular epithelial cells.

CAS / MDL: 7790-84-3
研究领域: 毒理学 · 细胞生物学 · 肾脏生理学
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FOOD HYDROCOLLOIDS · 2022 · IF 11.504

Foaming and interfacial properties of desalted duck egg white nanogels after weak enzymatical hydrolyzation

Bin Zhou,Jingyun Zhao,Yujuan Rong,Mengchen Li,Hongshan Liang,Bin Li,Jing Sun

Gel-assisted desalination is an efficient way of desalting salted duck egg whites , but results in severe loss of egg white functional properties, especially foaming properties. Here, slightly enzymatical hydrolyzation was used as a promising method to improve the interfacial properties of desalted duck egg white nanogels (DEWN). After partial hydrolysis (ranging from 2.27% to 4.22%), the foaming capability (FC) of the mixture could reach more than 200%, much higher than that of unhydrolyzed DEWN (about 30%). After separated examination, such improve FC was dominantly attributed to the supernatant, which also impacted enhanced foaming stability (FS). The adsorption of the supernatant and the resulting surface rheological measurements exhibited that the extension of the hydrolysis time can increase the diffusion and adsorption rate to the interface, performing high elasticity. Specially, the Lissajous curves displayed that the samples with short enzymolysis time first appeared asymmetric shape, indicating that extending enzymolysis time could improve the elasticity of the interface, which contributed to the improvement in foaming properties. As in a wide range of pH values, the FC and FS of the supernatant performed well, especially exhibited excellent FS at pH 5.0. In general, pepsin hydrolysis treatment, as an oversimplified and green modification strategy could significantly improve the foaming performance of DEWN, and solve the problem of loss in its foamability of salted duck egg whites obtained after desalination treatment.

研究领域: 食品科学 · 生物化学
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Journal of Environmental Chemical Engineering · 2020 · IF 4.3

Upcycling discarded duck down into highly porous and nitrogen/oxygen dually doped graphitic carbon for admirable energy and CO2 storage

Xiaojie Bai,Zuyou Zhuo,Xiuchun Deng,Biao Huang,Mingjie Huang,Yandan Chen

The convenient fabrication of heteroatom self-doped porous carbons from various biomasses has become a hot topic due to their unique advantages in environmental and energy-related applications. In this study, N/O self-doped and sheet-like graphitic porous carbons were successfully prepared by straightforward catalytic activation of recycled duck down with mixed ZnCl 2 -FeCl 3 salts. During the synthesis, FeCl 3 exerts impressive influence on the microstructure of the derived carbons. The obtained porous carbon can deliver an impressive gravimetric capacitance of 222 F g −1 along with powerful retention rate of 80% up to 10 A g −1 , as well as all-right capacity preservation of 109% after 5,000 cycles, owing to the favorable textural structure with readily accessible active surface area, rapid electrolyte transport channels, moderate graphitization level, and advantageous surface chemistry of rich heteroatoms co-doping. Additionally, the as-synthesized carbons also exhibit superior CO 2 uptake capacity up to 4.4 mmol g −1 under ambient conditions. The stored CO 2 in the carbons can be readily recycled into Na 2 CO 3 by impregnation with NaOH solution towards sustainable carbon sequestration . The present work affords a feasible strategy for massive reutilization of discarded duck down to manufacture advanced active carbons for applications including energy storage systems and CO 2 capture.

CAS / MDL: 7647-01-0
研究领域: 材料科学 · 环境科学 · 能源科学
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