INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Jianmei Sun,He Bao,Yajie Peng,Haimin Zhang,Ya Sun,Jiajun Qi,Hailong Zhang,Yang Gao
This study aims to evaluate the effects of Gelucire44/14 on the in vitro transport, in situ intestinal absorption, as well as in vivo antidiabetic efficacy of berberine (BBR). In the in vitro study, Gelucire44/14 (0.1%, v/v) increased the absorptive transport of BBR across the intestinal membrane of a rat and reduced the relative transport in the secretory direction, thus demonstrating its potential inhibitory effect on intestinal P-glycoprotein (P-gp). In the in situ absorption study, Gelucire44/14 (0.1%, v/v) increased BBR absorption, and this enhancing effect was more significant in the ileum than in the colon of a rat. Oral delivery of BBR with Gelucire44/14 (0.1%, v/v) to diabetic mice, compared with the BBR group, induced a significant hypoglycemic effect on day 7 and day 12 after administration. This result was well correlated with the results of the in vitro study, indicating the important contribution of the P-gp inhibitory effect of Gelucire44/14 to the improvement of the antidiabetic efficacy in vivo . In addition, Gelucire44/14 (0.1%, v/v) neither increased the levels of protein and lactate dehydrogenase in intestinal perfusion nor changed the morphology of the rat intestinal epithelium relative to those of the negative control. This finding suggested that 0.1% (v/v) Gelucire44/14 caused no apparent membrane damage to rat intestine. In conclusion, Gelucire44/14 exhibited potential for enhancing the oral absorption of BBR, thereby improving the antidiabetic efficacy of BBR.
研究领域:
药理学
·
糖尿病研究
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Tongqing Wu,Zhihui Hu,Jiaqi Yang,Yan Jia,Zekun Dong,Yu Tang,Yan Zhang
Textile wastewater was typically characterized by high concentrations of chloride ions, with Acid Red 14 (AR14) representing a significant pollutant. Nevertheless, research into the degradation of AR14 by electrochemical advanced oxidation processes (EAOPs) had largely overlooked the influence of chloride ions. In this regard, the EC-Chlorine-MMO/Ti system was designed, and the degradation mechanism of AR14 was subjected to meticulous investigation. The adopted anode was composed of Ti/IrO 2 -Ta 2 O 5 (mixed metal oxide, MMO). It demonstrated superior efficiency in degrading AR14 within a sodium chloride (NaCl) electrolyte, registering an apparent kinetic constant rate approximately 16.76 times greater than that observed within a sodium perchlorate (NaClO 4 ) electrolyte. The optimal chloride ion concentration was identified as 0.04 mol/L. Besides, alkaline conditions were not conducive to the degradation of AR14. It was noteworthy that the electrochemical byproducts chlorite (ClO 2 − ), chlorate (ClO 3 − ), perchlorate (ClO 4 − ), and trihalomethane (THMs) were not produced in the solution treated by the EC-Chlorine-MMO/Ti system. Electron paramagnetic resonance (EPR) spectroscopy, probe, and quenching experiments identified that hydroxyl radicals ( • OH), superoxide radicals (O 2 •− ), and dichloride radicals/chlorine monoxide radicals (Cl 2 •− / ClO • ) were the major reactive species. Of these, • OH played a pivotal role in AR14 degradation. Additionally, the three principal degradation pathways of AR14 were deduced by LC-MS analysis, UV–vis spectra, density-functional theory (DFT) calculations, and excitation-emission matrix (EEM) fluorescence spectroscopy. Furthermore, the generation mechanism of reactive species and the proposed mechanism of AR14 degradation by the EC-Chlorine-MMO/Ti system were presented. The study made a significant contribution to the understanding of the electrochemical reactions occurring in textile wastewater and provided valuable insights into the rational control of electrolytic conditions for the treatment of saline wastewater.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
·
2015
·Vol. 35
·期 12
·1473-1480
·
IF 2.982
Bing Shen,Hong-Cui Liu,Wen-Bin Ou,Grant Eilers,Sheng-Mei Zhou,Fan-Guo Meng,Chun-Qi Li,Yong-Quan Li
Basic Violet 14, Direct Red 28 and Acid Red 26 are classified as carcinogenic dyes in the European textile ecology standard, despite insufficient toxicity data. In this study, the toxicity of these dyes was assessed in a zebrafish model, and the underlying toxic mechanisms were investigated. Basic Violet 14 and Direct Red 28 showed acute toxicity with a LC 50 value at 60.63 and 476.84 µg ml –1 , respectively, whereas the LC 50 of Acid Red 26 was between 2500 and 2800 µg ml –1 . Treatment with Basic Violet 14, Direct Red 28 and Acid Red 26 resulted in common developmental abnormalities including delayed yolk sac absorption and swimming bladder deflation. Hepatotoxicity was observed in zebrafish treated with Basic Violet 14, and cardiovascular toxicity was found in zebrafish treated with Acid Red 26 at concentrations higher than 2500 µg ml –1 . Basic Violet 14 also caused significant up-regulation of GCLC gene expression in a dose-dependent manner whereas Acid Red 26 induced significant up-regulation of NKX2.5 and down-regulation of GATA4 at a high concentration in a dose-dependent manner. These results suggest that Basic Violet 14, Direct Red 28 and Acid Red 26 induce developmental and organ-specific toxicity, and oxidative stress may play a role in the hepatotoxicity of Basic Violet 14, the suppressed GATA4 expression may have a relation to the cardiovascular toxicity of Acid Red 26. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
毒理学
·
分子生物学
·
发育生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2025
·
IF 9.8
Xifan Li,Guijing Chen,Xin Li,Zhaoyang Song,Wen Liu,Baicang Liu
The flourishing growth of electric vehicle market has led to pile of spent lithium-ion batteries (LIB), efficient treatment and recovery of retired LIBs is crucial for ecological environment and human health, especially the terminal treatment of spent lithium-ion battery raffinate (SLR). For selectively recycle Li + from SLR (the lithium and sodium concentration reaches up to 1000 mg/L and 31,200 mg/L, respectively), dibenzo 14-crown-4 ether (DB14C4) is synthesized as extractant for lithium due to the excellent size-match effect. The extraction performance of DB14C4 is evaluated from aspects of dosage, temperature, organic/aqueous phase (O/A) ratio, adsorption kinetics, adsorption selectivity and reusability through liquid-liquid extraction. The results manifested that DB14C4 exhibited fast adsorption kinetics and reached saturation in 1 h, and reached the highest adsorption capacity of 22.1 mg/g with concentration of 0.03 mol/L, O/A ratio of 1:4 and temperature of 25 °C. Besides, DB14C4 has excellent selective adsorption for Li + with lithium partition coefficient of 10.2 mL/g and Li + /Na + separation factor of 13.2. Furthermore, the selectivity of DB14C4 to Li + is studied by density functional theory calculation, including structure optimization, molecular orbital analysis, charger transfer and natural bond orbital (NBO) analysis. The results show that DB14C4 preferentially complex with Li + with lithium ion lies in the center plane of crown ether ring, and the DB14C4-Li + complex has higher charge transfer and bigger stabilization energy than DB14C4-Na + . This paper provides a feasible method for lithium recovery from SLR to guarantee sustainable lithium supply for electric vehicles, and offers brand-new perspectives to evaluate the full life cycle of LIBs.
研究领域:
环境科学
·
材料化学
·
计算化学
✓ 已验证
产品关联
DOI
高影响因子
Pharmaceutics
·
2023
·Vol. 15
·期 12
·2784
·
IF 5.4
Jixue Yang,Yitong Huo,Xin Jin,Meiyun Liu,Yuting Lu,Lingman Ma,Changlin Zhou,Taijun Hang,Min Song
The interactions between active pharmaceutical ingredients (APIs) and excipients may lead to API degradation, thereby affecting the safety and efficacy of drug products. Cbf-14 is a synthetic peptide derived from Cathelicidin-BF, showing potential for bacterial and fungal infections. In order to assess impurities in Cbf-14 gel, we developed a two-dimensional liquid chromatography coupled with quadrupole/time-of-flight mass spectrometric method. A total of eleven peptide degradation impurities were identified and characterized. Furthermore, the compatibility tests were conducted to evaluate the interactions of Cbf-14 with glycerol and methylcellulose, respectively. The results revealed that the impurities originated from condensation reactions between Cbf-14 and aldehydes caused by glycerol degradation. Several aldehydes were employed to validate this hypothesis. The formation mechanisms were elucidated as Maillard reactions between primary amino groups of Cbf-14 and aldehydes derived from glycerol degradation. Additionally, the compatibility of Cbf-14 with glycerol from different sources and with varying storage times was investigated. Notably, the interaction products in the gel increased with extended storage time, even when fresh glycerol for injection was added. This study offers unique insights into the compatibility study of peptides and glycerol, contributing to the ongoing quality study of Cbf-14 gel. It also serves as a reference for the design of other peptide preparations and excipients selections.Keywords:Cbf-14;peptide preparation;glycerol;aldehydes;Maillard reaction;compatibility study;2D LC–QTOF-MS/MS
研究领域:
药学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Chemical Neuroscience
·
2023
·
IF 5
Yu-Qing Liu,Chu-Qiao Liang,Zhi-Wei Chen,Jun Hu,Jin-Jian Hu,Yun-Yi Luo,Yong-Xiang Chen,Yan-Mei Li
Tau plays a major role in Alzheimer’s disease (AD) and several other neurodegenerative diseases. Tau undergoing liquid–liquid phase separation (LLPS) performs specific physiological functions, induces pathological processes, and contributes to neurodegeneration. Regulating Tau phase separation helps maintain physiological functions of Tau and inhibits pathological aggregation. Here, we show that the 14-3-3 zeta isoform (14-3-3ζ) participates in Tau LLPS. 14-3-3ζ can undergo co-phase separation with WT Tau, participate in and stabilize Tau droplets, and inhibit Tau droplet-driven tubulin assembly. On the other hand, 14-3-3ζ disrupts the LLPS of phosphorylated and glycated Tau, thereby inhibiting the amyloid aggregation initiated by LLPS.
研究领域:
神经科学
·
分子生物学
·
细胞生物学
·
神经退行性疾病
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2014
·Vol. 66
·期 10
·1410-1420
·
IF 2.161
Zhang Hailong,Huang Xiaoyan,Mi Jie,Huo Yayu,Wang Guan,Xing Jianfeng,Gao Yang
ObjectivesThis study aims to evaluate the absorption-enhancing effects of Gelucire 44/14 on the pulmonary absorption of different poorly absorbable drugs and relative mechanism of action.MethodsAbsorption-enhancing effect of Gelucire 44/14 were examined by an in-vivo pulmonary absorption experiment in rats, and the membrane toxicity of Gelucire 44/14 was evaluated by measuring levels of protein and dehydrogenase (LDH) in the bronchoalveolar lavage fluid (BALF) and morphological observation.Key findingsPulmonary absorptions of fluorescein isothiocyanate-dextrans, insulin and calcitonin were enhanced by Gelucire 44/14 (0.1–2.0%, w/v) in a concentration-dependent manner, and the maximal absorption-enhancing effect was obtained when the concentration of Gelucire 44/14 increased to 2.0% (w/v). Furthermore, Gelucire 44/14 neither increase the levels of protein and LDH in BALF nor change morphology of lung compared with control group. In addition, a well correlation between the absorption-enhancing effect and surface tension of insulin solution in the presence of Gelucire 44/14 was observed, suggesting Gelucire 44/14-mediated decrease in the surface tension of the gas-liquid interface in alveolar tissue was possible one of the improving mechanisms of Gelucire 44/14.ConclusionGelucire 44/14 was a potential and safe absorption enhancer for improving the absorption of poorly absorbable drugs including insulin and calcitonin by pulmonary delivery.
研究领域:
药剂学
·
药理学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2021
·Vol. 8
·期 24
·5277-5285
·
IF 6.569
Shiqing Li,Lu Han,Zhonglin Zhao,Hao Xu,Jingang Jiang,Peng Wu
The lamellar precursor of the FER type zeolite (PREFER) was applied to develop the interlayer expanded zeolite IEZ (interlayer-expanded zeolite)-FER (D4h) with extra-large pores through hydrothermal acid treatment-assisted molecular alkoxysilylation, using 1,3,5,7-tetramethyl cyclotetrasiloxane (D4h) as a silylating agent to interconnect neighboring zeolitic layers. The interlayer expanded structure was well preserved after calcination at 823 K, yielding a hydrophilic material denoted as IEZ-FER(D4h-B). The as-made IEZ-FER(D4h) exhibits expanded interlayer spacing along the a-axis. The 4-membered rings (4MR) exist in IEZ-FER(D4h) as the secondary structure building units, while the methyl groups therein attached result in the hydrophobic properties. The newly formed channel systems are composed of intersecting 14MR and 12MR pores along the [001] and [010] directions, respectively, which are larger than those of the conventional 3D FER. The powder XRD patterns determine that IEZ-FER(D4h) has a crystalline structure with the IMM2 space group and unit cell parameters of a = 28.0285(39) Å, b = 14.0921(6) Å, c = 7.4395(3) Å.
研究领域:
化学
·
材料科学
·
催化
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Feidong Yang,Lei Li,Junyuan Hua,Jintao He,Xiaohua Ma,Jianxin Li
To meet the growing demand for lithium resources, it is ideal to develop a green and efficient approach for recovering lithium resources from discarded lithium-ion batteries. In this work, nanofiber membranes of 14-crown-4-ether polyimide (14C4PI) (M n = 33 kDa) with the crown ether loading of 1.38 mmol g −1 in the main chain were fabricated by electrospinning and the structure of the nanofiber membrane was regulated by tuning the electrospinning parameters. The selectivity adsorption of 14C4PI nanofiber membrane was investigated in the simulated extract of discarded lithium-ion batteries. The results showed that the 14C4PI-12 nanofiber membrane with a high specific surface area of 41.27 m 2 /g and a diameter of 122.7 nm was successfully prepared under the operating conditions (polymer concentration of 12 wt%, voltage of 30 kV, flow speed of 1 mL h −1 , tip-to-collector of 100 mm). The 14C4PI-12 nanofiber membrane displayed a high equilibrium adsorption capacity for Li + (Q m = 40.17 mg g −1 ). The adsorption of Li + onto the membrane was proven to be monolayer chemical adsorption confirmed by adsorption kinetics and isotherm models. The calculated equilibrium adsorption rate (EAR) was up to 70 % and the complex-ratio of crown ether in 14C4PI to Li + was 1.46:1, whose theoretical value is 1:1. Meanwhile, the selective separation factor of Li + to Na + , K + , Mn 2+ , Co 2+ , and Ni 2+ were up to 52.3, 54.97, 48.82, 41.05 and 35.04, respectively, which were much higher than that obtained from other selective adsorption method reported in literatures. The density functional theory (DFT) calculations confirm that the superior selectivity of 14C4PI to Li + is attributed to the higher Gibbs free energy change of 14C4PI-Li + complexation (−113.99 kcal mol −1 ). To sum up, this work provides an efficient method to fabricate crown ether functional polymer nanofiber membrane for recycling lithium resources.
研究领域:
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Food Quality and Safety
·
2024
·
IF 3
Fu Hanyue,Fan Dancai,Wang Jin,Kou Ruixin,Wang Yuanyifei,Wu Yuekun,Wang Shuo
ObjectivesConstipation is a prevalent gastrointestinal issue, and the efficacy of probiotics in alleviating constipation has been well proved. This study aimed to investigate the impact of Bifidobacterium longum subsp. infantis NKU FB3-14 on loperamide- induced constipation by focusing on improving intestinal barrier function and modulating gut microbiota compositionMaterials and MethodsThe constipated model mice induced by loperamide were treated with NKU FB3-14, and the laxative effect was assessed based on the fecal water content, first black stool time and gastrointestinal transit rate. Gastrointestinal regulatory peptides in serum, intestinal neurotransmitter and inflammatory cytokines in colon tissues were measured using ELISA kits. Changes of gut microbiota composition were analyzed through 16S ribosomal DNA sequencing identification. Additionally, HPLC detection was performed to quantify short chain fatty acids levels in fecesResultsTreatment with NKU FB3-14 led to increase the fecal water content, shorten the first black stool time and improve small intestine transit rate. Motilin and substance-P significantly decreased in the model group, and only motilin increased in FB3-14 group; somatostatin and vasoactive intestinal peptide were decreased in model mice and both increased in FB3-14 group; 5-HT levels in the colon tissue were upregulated following NKU FB3-14 treatment. Histological examination revealed thinner colonic mucosa in the model group along with significant in TNF-α, IL-1β and IL-17 levels form the colon tissues which were all relieved by NKU FB 3-14 treatment. Furthermore, NKU FB3-14 intervention resulted in reduced abundance of Desulfobacterota and Desulfovibrio while increasing the abundance of Ruminococcaceae and Eubacterium; higher level of butyric acid was observed in fecesConclusionsIn summary, our findings demonstrated that NKU FB3-14 treatment significantly enhanced the intestinal motility, regulated the expression levels of gastrointestinal regulatory peptides, prevented damage to colonic barriers as well as ameliorated gut microbiota imbalance associated with loperamide-induced constipation.
研究领域:
胃肠病学
·
微生物学
·
药理学
·
益生菌
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
·
IF 5.065
Xiaoxing Lu,Shaoxia Xie,Jian Kuang,Hujun Xie
A comprehensive experimental investigation on the microstructures of microemulsion constructed with Gemini surfactant has been carried out. The structural transition of the microemulsion system, 14-4-14/1-propanol/ n -heptane/water, from water-in-oil (W/O) to bi-continuous (B.C.) has been characterized by electrical conductivity and cyclic voltammetry . Being a typically percolative system, spherical nanodroplets are observed in the W/O system, while interlinks of water and oil continuous phases are observed in the middle phase, as confirmed by cryo-TEM. Most importantly, the role of water is clearly revealed. Increasing the water content results in size enhancement as well as improvement of micropolarity and diffusion behavior. Results of DSC measurements suggest that free water dominates only at high total water content. The unfrozen water which is not detected by DSC takes a considerable amount in the W/O system at low total water content, while the interphasal water reaches over 50% of total water in the middle phase system.
研究领域:
胶体化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Chengzhi Liu,Haoxin Ye,Jian Kuang,Gerui Ren,Qing Shen,Hujun Xie,Qunfang Lei,Wenjun Fang
The interfacial composition and thermodynamic parameters of W/O 14-n-14/alcohol/oil/water microemulsions were investigated by dilution method in terms of the number of linking groups n (n = 3–6), water content, alcohol and oil types. The gemini/alcohol/oil/water microemulsion system formed spontaneously with negative Gibbs free energy at 25 ℃. The long carbon chain of alcohol facilitated the formation of W/O microemulsion. With increasing carbon chain length of the oil phase, the absolute value of the Gibbs free energy initially increased and subsequently decreased. With an increasing number of linking groups in gemini surfactants, the amount of alcohol that was required in the interfacial layer increased, thereby hindering alcohol movement from the continuous phase to the interface layer, and the absolute value of the Gibbs free energy also decreased, suggesting that a small value of n is beneficial to the stability of microemulsions.
研究领域:
胶体化学
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）