Journal of Water Process Engineering
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2024
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IF 6.3
Xiaolin Pi,Xueni Zhang,Yue Tang,Changjiang He,Lijun Luo,Siping Ji,Fengzhi Jiang
Biochar (BC) composite photocatalysts have attracted much attention by virtue of their high efficiency in removing endocrine disruptors (EDCs). However, there has been a lack of systematic research on the mechanism and constitutive relationship of BC on photocatalysts, which has seriously impeded the development process in this field. In this study, Bi 2 WO 6 –BC 2% composite photocatalysts were prepared by microwave-assisted hydrothermal method and used for degradation of 17α-ethinylestradiol (EE2) and 17β-estradiol (E2) in conjunction with persulfate synergy (PS). The removal rate of EE2 and E2 by Bi 2 WO 6 –BC 2% was as high as 99.41 % (12 min) and 97.84 % (15 min), respectively. Systematic studies have shown that BC enhances the catalytic activity mainly through the following ways, increasing the specific surface area of the photocatalytic system, enriching the types of functional groups, enhancing the separation ability of photogenerated electron–hole pairs, and promoting the concentration of active free radicals. The results of the phytotoxicity experiments showed that compared to the EE2 or E2 pollutant solutions, the stem and root lengths of V. radiata increased by 93.63 % and 389.58 % in EE2 metabolites, and by 117.35 % and 160.38 % in E2 metabolites. Similarly, the stem and root lengths of P. vulgaris increased by 80.08 % and 174.91 % in EE2 metabolites, and by 188.92 % and 345.75 % in E2 metabolites. This work not only fills the gap in the study of the mechanism of action of BC, but also provides a green catalyst with very excellent photocatalytic efficiency and detoxification properties.
研究领域:
环境科学
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光催化技术
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毒理学
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高影响因子
Genes
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2024
·Vol. 15
·期 5
·605
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IF 2.8
Haijun Huang,Yuyan Liu,Qian Wang,Caichao Dong,Le Dong,Jingjing Zhang,Yu Yang,Xiancai Hao,Weijing Li,Ivana F. Rosa,Lucas B. Doretto,Xuebin Cao,Changwei Shao
It is widely known that all-female fish production holds economic value for aquaculture.Sebastes schlegelii, a preeminent economic species, exhibits a sex dimorphism, with females surpassing males in growth. In this regard, achieving all-female black rockfish production could significantly enhance breeding profitability. In this study, we utilized the widely used male sex-regulating hormone, 17α-methyltestosterone (MT) at three different concentrations (20, 40, and 60 ppm), to produce pseudomales ofS. schlegeliifor subsequent all-female offspring breeding. Long-term MT administration severely inhibits the growth ofS. schlegelii, while short term had no significant impact. Histological analysis confirmed sex reversal at all MT concentrations; however, both medium and higher MT concentrations impaired testis development. MT also influenced sex steroid hormone levels in pseudomales, suppressing E2 while increasing T and 11-KT levels. In addition, a transcriptome analysis revealed that MT down-regulated ovarian-related genes (cyp19a1aandfoxl2) while up-regulating male-related genes (amh) in pseudomales. Furthermore, MT modulated the TGF-β signaling and steroid hormone biosynthesis pathways, indicating its crucial role inS. schlegeliisex differentiation. Therefore, the current study provides a method for achieving sexual reversal using MT inS. schlegeliiand offers an initial insight into the underlying mechanism of sexual reversal in this species.
研究领域:
水产养殖
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内分泌学
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分子生物学
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生理学
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Jingye Cui,Huan He,Wanqi Shi,Zhicheng Liao,Danni Cui,Jianxiang Yang,Yixin Liu,Chaochao Lai,Bin Huang,Xuejun Pan
Microbial-derived extracellular polymeric substances (EPS) and iron minerals are ubiquitous in aquatic environments, and they can influence the fate of organic micropollutants such as 17α-ethinylestradiol (EE2). However, the interactions between EPS and iron minerals, and their influence on EE2 photodegradation, are seldom addressed in the literature. This study explored the effects of EPS derived from different aerobic or anaerobic microbials on the reductive dissolution of ferrihydrite (Fhy) and subsequent EE2 photodegradation, with emphasis on the impact of Fe-EPS complexes formation. In the dark, EPS could promote the reductive dissolution of Fhy via ligand-metal charge transfer, accompanied by Fe(III)-EPS complexes formation. Irradiation could further enhance such a dissolution process. Dissolved iron could accelerate the photolysis of protein and humic substances, triggering Maillard-like reactions that form the unsaturated medium- or high-molecular-weight polymers. The aerobic-EPS containing more proteins and humic substances photodegraded EE2 more effectively than anaerobic-EPS, with the triplet excited state playing a crucial role. In contrast, Fhy inhibited EPS-mediated EE2 photodegradation via light-shielding effect or photosensitizers oxidative decomposition. The information obtained in this research would contribute to the understanding of photochemistry involving EPS, iron minerals, and steroid estrogens, providing new insights into their environmental behavior.
研究领域:
环境化学
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光化学
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环境科学
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微生物学
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地球化学
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Chuan-hua Yang,Yujie Cui,Zhengxi Chen,Yiluo Hu,Xuejun Pan,Siping Ji,Feng-zhi Jiang
Peroxymonosulfate (PMS)-based advanced oxidation processes (PMS-AOPs) currently face issues regarding transition-metal ion valence conversion and practical application in water treatment. To address them, we synthesized a CoOOH catalyst with a unique three-dimensional porous flower-like morphology for PMS activation to degrade 17α-ethinylestradiol (EE2) in wastewater. By adding PMS to CoOOH in small amounts, 95.68 % of EE2 (3 mg/L) was degraded within 9 min. The catalytic efficiency of CoOOH was considerably higher than those of a material synthesized via the conventional precipitation method and commercial Co 3 O 4 . CoOOH also exhibited a wide pH adaptability range, good cyclic stability, and a low cobalt ion leaching concentration. It demonstrated excellent PMS activation performance, chemical stability, and safety. Mechanistic studies revealed that numerous surface hydroxyl groups and abundant oxygen vacancies in CoOOH facilitated efficient electron transfer, rapidly achieved the Co 3+ /Co 2+ redox cycle, and generated a large amount of highly oxidative nonradical ( 1 O 2 ) and radical (•O 2 − ) species, thereby efficiently degrading organic pollutants. Finally, by combining PMS-AOPs with membrane separation, a CoOOH/PTFE membrane–PMS system was fabricated for continuous EE2 degradation in lake water; it could operate continuously for 24 h in treating 1.25 L of Dianchi Lake water (Kunming, Yunnan) containing 3-mg/L EE2 while maintaining an EE2 removal rate of 92.35 %. The synthesized CoOOH/PTFE catalytic membrane facilitated transition-metal ion redox cycling and efficient organic-pollutant degradation as well as enabled convenient catalyst recovery, thereby reducing secondary pollution in the degradation process. Overall, this research provides a feasible strategy for the practical application of PMS-AOPs.
研究领域:
环境科学
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化学工程
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催化
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水处理技术
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材料科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Lijun Luo,Demei Meng,Lijin He,Xiaoxia Wang,Lihong Xia,Xuejun Pan,Fengzhi Jiang,Hongbin Wang,Jianhui Dai
A new porous C 3 N 4 /reduced graphene oxide/TiO 2 nanobelt catalyst (CRT) was assembled and used to remove 17α-ethinylestradiol (EE2) in the presence of peroxydisulfate (PS) and visible light. The characterization results showed that larger porous graphite carbon nitrides (pg-C 3 N 4 ) covered onto the surface of TiO 2 /reduced graphene oxide (RGO), in which layered RGO was wrapped onto the surface of anatase TiO 2 nanobelt uniformly. The layered RGO in CRT played significant role in separation of electron-hole pairs and nonradical activation. The electron paramagnetic resonance and quenching experiment revealed that h + , 1 O 2 and •O 2 – and direct electron transfer route contributed to complete removal of 6 mg/L EE2 within 6 min only with 0.5 g/L dosage and 1 mM PS. The degradation rate of EE2 in CRT/PS/Vis was 207.51 times than that in photocatalysis. The degradation products were identified by a solid phase extraction pretreatment method combined with ultra-high-performance liquid chromatography-mass spectrometry, and possible degradation pathways were proposed. Moreover, the estrogenic activities can be rapidly eliminated in CRT/PS/Vis process.
研究领域:
环境科学
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材料科学
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化学
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水处理
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
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IF 7.963
Huan He,Yanting Lin,Xiaoxia Yang,Xintong Zhu,Wenxiao Xie,Chaochao Lai,Shanshan Yang,Zhe Zhang,Bin Huang,Xuejun Pan
17 alpha-ethinylestradiol (EE2) in natural waters can seriously harm ecosystems and human health. Dissolved organic matter (DOM) and iron minerals are ubiquitous in natural waters, and they can shorten the half-life of EE2 in the natural environment. The interaction between dissolved organics and iron affects pollutants' transformation pathways. The mechanism of EE2's adsorption on hematite, magnetite and pyrite was studied. A photo-Fenton system was constructed in which humic acid (HA) and iron minerals degraded EE2 under simulated natural light conditions. Pyrite showed the best adsorption and degradation in acidic conditions (52%) for 5 h. Hydroxyl radical was found to be the main active substance in the photodegradation . The degradation products of EE2 were identified and possible degradation pathways were inferred. These results can contribute to the understanding of the transformation pathways of persistent organic pollutants in natural waters.
研究领域:
环境科学
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生态学
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水污染治理
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2023
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IF 6.9
Huan He,Min Shi,Xiaoxia Yang,Juhong Zhan,Yanting Lin,Ziwei Guo,Zhicheng Liao,Chaochao Lai,Xiaomin Ren,Bin Huang,Xuejun Pan
Dissolved organic matter (DOM) and iron minerals widely existing in the natural aquatic environment can mediate the migration and transformation of organic pollutants. However, the mechanism of interaction between DOM and iron minerals in the microbial degradation of pollutants deserves further investigation. In this study, the mechanism of 17 alpha-ethinylestradiol (EE2) biodegradation mediated by humic acid (HA) and three kinds of iron minerals (goethite, magnetite, and pyrite) was investigated. The results found that HA and iron minerals significantly accelerated the biodegradation process of EE2, and the highest degradation efficiency of EE2 (48%) was observed in the HA-mediated microbial system with pyrite under aerobic conditions. Furthermore, it had been demonstrated that hydroxyl radicals (HO•) was the main active substance responsible for the microbial degradation of EE2. HO• is primarily generated through the reaction between hydrogen peroxide secreted by microorganisms and Fe(II), with aerobic conditions being more conducive. The presence of iron minerals and HA could change the microbial communities in the EE2 biodegradation system. These findings provide new information for exploring the migration and transformation of pollutants by microorganisms in iron-rich environments.
研究领域:
环境科学
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微生物学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Xuan Zhang,Changling Fang,Xiaoyi Lou,Guangxin Yang,Cong Kong,Yongfu Shi,Dongmei Huang
17α-methyltestosterone (MT) is widely used in aquaculture in order to increase harvest yield. However, illegal addition of MT is potentially toxic to human. A simple and rapid method for the determination of MT in aquatic products was established on HPLC-MS/MS. Sample preparation was optimized using simple liquid–liquid extraction techniques with ethyl acetate and n -hexane. The analyte was separated on Capcell PAK C 18 MG II (100 mm × 2.0 mm, 5 µm), with a solution of 0.1 % formic acid in water and acetonitrile, and analyzed with multiple-reaction monitoring (MRM) mode on HPLC-MS/MS. Good linearity with high correlation coefficient (R 2 ≥ 0.9997) was obtained between 2 and 200 µg/L. The limits of detection (LODs) and quantitation (LOQs) were 0.4 and 1 µg/kg respectively. Good recoveries were demonstrated between 86.4 and 104.6 %. The precisions of inter-day and intra-day were validated and the results indicated acceptable stability and reproducibility. The analyte showed stable up to 30 days storage at −20 °C. Samples purchased from Shanghai market for each matrix (shrimp, Chinese mitten crab, tilapia, eel, bullfrog and mussel) were tested for method application. In summary, the proposed method was validated for MT determination in aquatic products.
研究领域:
分析化学
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食品安全
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水产养殖
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质谱分析
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DOI
高影响因子
Aquaculture Reports
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2026
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IF 3.8
Caichao Dong,Yuyan Liu,Xuebin Cao,Jingjing Zhang,Xuwen Cao,Lei Lin,Jiliang Lin,Meng Wang,Jiashuo Di,Jie Wu,Lili Zhang,Qian Wang,Changwei Shao
Sex-controlled breeding holds significant value in aquaculture. By controlling the sex of fish, faster-growing sexes can be preferentially produced, shortening the rearing time to market size. Sebastes schlegelii exhibits a significant female growth advantage, and thus all-female aquaculture can substantially improve economic benefits. The objective of this study was to induce XX pseudo-males in S. schlegelii using exogenous 17α-methyltestosterone (MT) and letrozole (LZ) for all-female breeding, and to clarify the underlying hormonal and transcriptomic mechanisms. S. schlegelii juveniles before sex differentiation were treated with MT at concentrations of 20, 40, 60 mg/kg and LZ at concentrations of 300, 600, 900 mg/kg for 30 and 40 days, respectively. Phenotypic and histological analysis showed that all treatment groups achieved 100 % sex reversal efficiency, with pseudo-males successfully induced. Growth performance results indicated that the 30-day treatment with MT (20 mg/kg) or LZ (300 mg/kg) had no significant difference from the untreated control group, while higher concentrations and longer treatment durations inhibited growth and testicular development. Endocrinology showed that the serum steroid levels (elevated testosterone and 11-ketotestosterone, reduced estradiol) of pseudo-males were closer to those of normal males. Transcriptome analysis showed that the downregulation of genes related to ovarian development ( cyp19a1a , foxl2 ) and the upregulation of genes related to testicular development ( amh , sox9 , dmrt1 ) jointly regulated sex reversal, and the pseudo-males induced by both hormones had enriched steroid hormone biosynthesis and TGF-β signaling pathways. This study establishes a feasible sex reversal strategy in S. schlegelii and provides molecular insights into gonadal differentiation, offering a technical basis for all-female breeding.
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Kejian Tian,Jinming Gu,Lang Xie,Dandan Zhou,Fanxing Meng,Pengfei Hao,Yue Yu,Fan Li,Hongliang Huo,Shi Xie
17α-Ethynylestradiol (EE2) is a ubiquitous synthetic estrogen of global concern. Due to its recalcitrance to biodegradation, EE2 is often insufficiently removed during wastewater treatment and consequently released with effluents, posing significant ecological risks and potential threats to human health. In this study, Rhodococcus equi DSSKP-R-001 ( R. equi -001) was identified as one of the most potent EE2-degrading bacterium, achieving complete degradation of EE2 at low concentrations. Transcriptomic analysis revealed substantial upregulation and high functional similarity of genes encoding short-chain dehydrogenase/reductase (SDR) during EE2 degradation. Among these candidates, sdr R was functionally validated as the most catalytically efficient, achieving 97.88 % EE2 removal. Molecular docking analyses demonstrated that the sdr R -mediated conversion of EE2 to estrone (E1) is facilitated by multiple key amino acid residues through substrate binding, hydrogen bonding, and hydrophobic interactions. Furthermore, the sdr R -harboring genetically engineered bacterium (GEB) exhibited superior biodegradation potential, achieving up to 96.48 % EE2 removal individually and enabling complete elimination when applied for bioaugmentation. Concurrently, bioaugmentation with GEBs reshaped the sludge microbial community by optimizing interspecies interactions and increasing the abundance of denitrifying and phosphorus-accumulating microorganisms, thereby improving overall pollutant removal performance. Overall, this study expands the microbial and enzymatic repertoire for EE2 degradation and demonstrate the potential of genetic engineering strategies for improving the removal of recalcitrant micropollutants in biological wastewater treatment.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Danni Cui,Huan He,Wenxiao Xie,Shanshan Yang,Ziwei Guo,Zhicheng Liao,Feng Liu,Chaochao Lai,Xiaomin Ren,Bin Huang,Xuejun Pan
17α-ethinylestradiol (EE2), a synthetic endocrine-disrupting chemical, can degrade in natural waters where humic acids (HA) and dissolved iron (DFe) are present. The iron is mostly bound in Fe(III)-HA complexes, the formation process of Fe(III)-HA complexes and their effect on EE2 degradation were explored in laboratory experiments. The mechanism of ferrihydrite facilitated by HA was explored with results indicating that HA facilitated the dissolution of ferrihydrite and the generation of Fe(III)-HA complexes with the stable chemical bonds such as C-O, C O in neutral, alkaline media with a suitable Fe/C ratio. 1 O 2 , •OH, and 3 HA* were all found to be important in the photodegradation of EE2 mediated by Fe(III)-HA complexes. Fe(III)-HA complexes could produce Fe(II) and hydrogen peroxide (H 2 O 2 ) to create conditions suitable for photo-Fenton reactions at neutral pH. HA helped to maintain higher dissolved iron concentrations and alter the Fe(III)/Fe(II) cycling. The natural EE2 photodegradation pathway elucidated here provides a theoretical foundation for investigating the natural transformation of other trace organic contaminants in aquatic environments.
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Feiyuan Liu,Weiqing Ma,Zhicheng Liao,Chaochao Lai,Xiunan Cai,Huan He,Bin Huang,Xuejun Pan
DBC photobleaching involved fast, slow, and photoresistant fractions. • P-DBC exhibited higher oxidizability and bioavailability, but lower energy density. • Molecular intensity and H content governed DBC photochemical reactivity. • P-DBC promoted goethite-mediated anaerobic microbial transformation of EE2. • EE2 biotransformation involved de‑ethynylation and decarboxylation. Dissolved black carbon (DBC) is an important component of dissolved organic matter, which undergoes continuous photobleaching in the sunlit surface water. The resulting photobleached DBC (P-DBC) can subsequently be transported across the sediment-water interface into sediments rich in minerals and microbes, thereby regulating the transformation of organic contaminants. This study investigated photobleaching-induced molecular transformation of DBC and the mechanism of P-DBC in goethite-mediated 17α-ethinylestradiol (EE2) anaerobic biotransformation. The results showed that photobleaching promoted the oxidation and decarbonization of DBC, driving its molecular composition toward a higher oxidation state and lower energy density. Intensity and H content were the key molecular features governing the photochemical reactivity of DBC. P-DBC was enriched in oxygen-containing functional groups that could enhance electron transfer with goethite. Additionally, the higher bioavailability of P‑DBC enriched aromatic‑degrading bacteria. Species associated with iron reduction and organic matter transformation in the presence of goethite were increased. As a result, P-DBC combined with goethite significantly promoted the anaerobic biotransformation of EE2, achieving a removal efficiency of 73.0%. EE2 transformation involved de-ethynylation and decarboxylation to yield more oxygenated products, contrasting with co‑metabolism alone. These findings provide new mechanistic insights into the natural attenuation of estrogens in iron-rich aquatic environments. Download: Download high-res image (276KB) Download: Download full-size image
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高影响因子
危险品化学品经营许可证（带存储）