INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Junxin Zhao,Shichang Liu,Yifang Gao,Maomao Ma,Xianghui Yan,Ding Cheng,Dongman Wan,Zheling Zeng,Ping Yu,Deming Gong
Enzymatic degradation has become the most promising approach to degrading organic ester compounds. In this study, Bacillus licheniformis NCU CS-5 was isolated from the spoilage of Cinnamomum camphora seed kernel, and its extracellular lipase was purified, with a specific activity of 192.98 U/mg. The lipase was found to be a trimeric protein as it showed a single band of 27 kDa in SDS-PAGE and 81 kDa in Native-PAGE. It was active in a wide range of temperatures (5–55 °C) and pH values (6.0–9.0), and the optimal temperature and pH value were 40 °C and 8.0, respectively. The enzyme was active in the presence of various organic solvents, metal ions, inhibitors and surfactants. Both crude and purified lipase retained more than 80% activity after 5 h in the presence of commercial detergents, suggesting its great application potential in detergent industry. The highest activity was found to be towards medium- and long-chain fatty acids (C 6 -C 18 ). Peptide mass spectrometric analysis of the purified lipase showed similarity to the lipase family of B. licheniformis . Furthermore, it degraded more than 90% 2,4-D butyl ester to its hydrolysate 2,4-D within 24 h, indicating that the novel lipase may be applied to degrade organic ester pesticides.
研究领域:
生物化学
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环境科学
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生物技术
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微生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Yun Liu,Meng Xiao,Ningxia Xu,Mengsu Yang,Changqing Yi
This study reports a new ratiometric fluorescence probe (referred to as [email protected] @bCDs) and a smartphone-based sensing system for on-site quantitation of Fe 3+ , ascorbic acid (AA), alkaline phosphatase (ALP) and 2,4-dichlorophenoxyacetic acid (2,4-D). [email protected] @bCDs with well-resolved dual emissions is facilely synthesized by covalent conjugation of red-emissive quantum dots (rQDs), the internal standard, with blue-emissive carbon dots (bCDs), the Fe 3+ -specific fluorescent indicator . 2,4-D can specifically inhibit the enzymatic activity of ALP, leading to suppress the production of AA which can reduce Fe 3+ to Fe 2+ and thereafter trigger the fluorescence response of [email protected] @bCDs. Taking advantages of these highly specific cascade catalytic reactions , ratiometric fluorescence schemes are established for sensing Fe 3+ , AA, ALP and 2,4-D both in aqueous solution and on paper-based microarray . The smartphone-based fluorescence detector integrated with the [email protected] @bCDs-based microarray demonstrated good sensing performance with a wide linear range and low detection limit, and is successfully applied to quantitate 2,4-D in cabbage and tap water samples. The reported method which enables point-of-need quantitation in a sample-in-result-out manner and highlights its features of cost-effectiveness and ease-of-use, is ideally for food safety inspection, environment surveillance, health monitoring at point-of-need, especially in low resource settings.
研究领域:
分析化学
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环境科学
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食品安全
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生物技术
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2020
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IF 9.038
Jingju Cai,Minghua Zhou,Xin Xu,Xuedong Du
Anode materials are crucial to anodic oxidation for wastewater treatment. In this regard, stable boron and cobalt co-doped TiO 2 nanotube (B, Co-TNT) was prepared for the first time, and its lifetime was found increased significantly while electrocatalytic activity decreased with the increase of Co(NO 3 ) 2 in preparation from 1 to 10 mM. Characterized by scanning electron microscope (SEM), X-Ray Diffraction (XRD) and X-ray Photo-electronic Spectroscopy (XPS), B and Co content were optimized and successfully doped on TNT, which was more smooth without ripple with Co content of 0.038 mg/cm 2 in a valence of +2, and B atomic content of 2.17 at.% in form of Ti-B-O. This optimized anode enhanced electrode lifetime 122.8 times while the electrochemical activity decreased slightly when compared to the undoped TNT. The effects of current density, initial pH and initial 2,4-dichlorophenoxyacetic acid (2,4-D) concentration were investigated, and the mainly responsible radical for degradation was confirmed to be the surface OH on B, Co-TNT anode. This anode had better performance on the TOC removal, mineralization current density (MCE) and energy consumption (Ec) when compared with BDD, PbO 2 , DSA and Pt anodes, and it also presented a very stable degradation for 10 cycles oxidation of 20 mg/L 2,4-D with allowable Co leaching. Therefore, B, Co-TNT anode is a promising, stable, safety and cost-effective anode for application in electrochemical advanced oxidation processes (EAOPs).
研究领域:
环境科学
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材料科学
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化学工程
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Tinyan Wei,Hongye Wu,Zhili Li
The coexistence of multiple endocrine disruptors (EDs) poses new challenges to conventional water treatment technologies . β-Cyclodextrin (β-CD), because of its water-soluble nature, is limited in application as an adsorbent material. In this work, lignin immobilized β-CD composite microspheres (CD-PLMs) were newly fabricated by a reverse-phase suspension method. SEM, FTIR , and XPS analysis showed that CD-PLMs had a good spherical profile and multifunctionalized surface chemistry (O/N-containing functional groups, hydrophobic benzene rings, and internal cavities). Two representative EDs (2,4-D, BPA) were selected as model pollutants to investigate the adsorption performance of CD-PLMs. CD-PLMs showed fast adsorption kinetics for the removal of 2,4-D and BPA, and the adsorption capacity according to the Langmuir model was 497.6 mg/g and 499.2 mg/g, respectively, which is much higher than previously reported activated carbon, graphene oxide, and MOF. Combining the zeta potential , kinetics, thermodynamics, FTIR, and XPS results, the adsorption mechanism was proposed. Due to the multifunctional properties of CD-PLMs, different active sites are available for different pollutants, CD-PLMs exhibited non-competitive adsorption for 2,4-D and BPA in binary systems. Moreover, the CD-PLMs had good reusability during multiple adsorption-desorption cycles. This study provides green materials for the treatment of complex water pollution and new ways to valorize biomass.
研究领域:
环境科学
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材料科学
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水处理技术
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化学工程
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产品关联
DOI
高影响因子
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