INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
·
2019
·
IF 1.267
Kai Sun,Shun-Yao Li,Hui-Ling Chen,Qing-Guo Huang,Youbin Si
In this study, we used a simple and rapid colourimetric reaction for visual sensing of Fe2+ and Pb2+ ions in water by employing nano-MnO2 as a natural oxidase mimic to respectively catalyse ABTS and TMB in citrate-phosphate buffer solution (C-PBS) at 25°C and pH 3.8. It was found that nano-MnO2 possessed highly oxidase-mimicking activity with the Km values of 0.030 and 0.027 toward ABTS and TMB, respectively, indicating TMB had a stronger affinity on nano-MnO2 than ABTS. Interestingly, the presence of 0.01 mmol·L−1 Fe2+/Pb2+ ion was able to significantly down-regulate the activity of MnO2 nanozyme in nano-MnO2-mediated ABTS reaction processes (P < 0.01), which mainly due to the strong adsorption of metal ion toward nano-MnO2 surface via the electrostatic attractions, thus leading to the passivation and inactivation of MnO2 nanozyme catalytic activity. Thereinto, Fe2+ reacted with multivalent manganese by oxidation-reduction, while Pb2+ was specifically adsorbed onto the surface of MnO2 nanozyme and formed complexes. Notably, only Fe2+ ion inhibited the activity of MnO2 nanozyme-TMB with a detection limit as low as 1.0 μmol·L−1. In MnO2 nanozyme-ABTS sensing systems, Fe2+ and Pb2+ ions detection limit of 0.5 and 2.0 μmol·L−1 were, respectively, achieved with a linear response range of 0–0.02 and 0–0.8 mmol·L−1, implying the developed MnO2 nanozyme-ABTS sensor was potentially applicable for the visual determination of Fe2+ and Pb2+ ions in water. In the real water samples, MnO2 nanozyme-ABTS achieved high accuracy (relative errors: 3.4‒10.5%) and recovery (96‒110%) for respective detection of Fe2+ and Pb2+ ions. The simple and rapid MnO2 nanozyme-ABTS sensing systems might provide a practical assay for visual detection of Fe2+ and Pb2+ ions in the environmental water samples.
研究领域:
分析化学
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环境科学
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生物催化
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产品关联
DOI
高影响因子
Chemical Engineering Journal Advances
·
2022
·
IF 0
Na Yuan,Hongchao Li,Jieshu Qian
Determination of the concentration of peracetic acid (PAA) in the presence of H 2 O 2 is a great challenging problem due to the similar chemical behaviors of the two species. In this study, we developed a new method for simultaneous measurement of PAA (0.5 – 10.0 μM) and total peroxides (i.e., PAA and H 2 O 2 with a molar ratio of 10:6.5), based on the catalytic oxidation of ABTS by separately using Co(II) and peroxidase (POD) as the catalysts. Owing to the unique catalytic activity of Co(II) towards PAA, the coexistent H 2 O 2 exhibits negligible effect on the determination of PAA. The value of absorbance at 415 nm increases linearly with increasing the concentration of PAA or total peroxides, and the sensitivities for PAA and total peroxides are as high as 5.31 × 10 4 and 9.07 × 10 4 M −1 cm −1 , respectively. In addition, the proposed ABTS method shows excellent feasibility and applicability, which make it suitable to be employed for detection in complicated water matrices.
研究领域:
分析化学
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环境科学
✓ 已验证
DOI
CHEMOSPHERE
·
2020
·
IF 5.778
Yifei Leng,Jianguo Bao,Henglin Xiao,Dandan Song,Jiangkun Du,Sanjeeb Mohapatra,David Werner,Jun Wang
The threat of antibiotics in the environment causing antibiotics resistance is a global health concern. Enzymes catalyze pollutant transformations, and how commercially available enzymes like horseradish peroxidase (HRP), with or without a redox mediator, may be used to degrade antibiotics in water treatment is of great interest. This work demonstrates tetracycline transformation by HRP, and how it is significantly enhanced by free radicals created from the mediator 2,2-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). Water temperature and pH strongly influence the tetracycline removal rate due to their correlation with the enzyme activity , abundance and stability of ABTS •+ . Four transformation products were identified in the pure HRP system using a liquid chromatography tandem mass spectrometry hybrid quadrupole-orbitrap mass spectrometer system. Addition of 25 μmol L −1 ABTS not only accelerated the degradation of tetracycline, but also expanded the range of degradation pathways. Potential tetracycline transformation pathways are proposed based on these observations, which include a range of mechanisms such as hydroxylation, demethylation, dehydration, decarbonylation and secondary alcohol oxidation. Despite of decreased efficiency, the HRP/ABTS system was able to degrade tetracycline in a domestic wastewater treatment plant effluent matrix, which demonstrates the potential of the system to be utilized in wastewater treatment.
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Chemistry
·
2015
·Vol. 2015
·期 1
·670194
·
IF 0.772
Youxun Liu,Mingyang Yan,Yuanyuan Geng,Juan Huang
Efficient reuse and regeneration of spent mediators are highly desired for many of the laccases’ biotechnology applications. This investigation demonstrates that a redox mediator 2,2′-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) covalently attached to silica nanoparticles (SNPs) effectively mediated dye decolorization catalyzed by laccase. Characteristics of ABTS-modified silica nanoparticles (ABTS-SNPs) were researched by scanning electron microscopy and Fourier-transformed infrared spectroscopy. When ABTS and ABTS-SNPs were used as laccase mediators, the decolorization yields of 96 and 95% were, respectively, obtained for indigo carmine dye. The results suggest that ABTS immobilized on SNPs can be used as laccase mediators as they retain almost the same efficiency as the free ABTS. The oxidized ABTS-SNPs were regenerated by their reduction reaction with ascorbic acid. Decolorization efficiency of regenerated ABTS-SNPs and their initial forms were found to be almost equivalent. Six reuse cycles for spent ABTS-SNPs were run for the treatment of indigo carmine, providing decolorization yields of 96–77%. Compared with free mediator, the immobilized mediators have the advantage of being easily recovered, regenerated, and reused making the whole process environmentally friendly.
研究领域:
环境生物技术
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纳米生物技术
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酶工程
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污染物降解
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绿色化学
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DOI
高影响因子
FOOD CHEMISTRY
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2025
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IF 9.8
Jing-Wen Hao,Wei-Ting Wang,Nai-Dong Chen,Yan Shen
The novel application of a combined screening method, utilizing AAPH, ABTS, and FRAP assays with HPLC, is first used for screening natural antioxidants in Green Calyx plums (GCP). This method was assumed that the peak areas of compounds with antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH, ABTS, and FRAP. The thirteen screened antioxidants can be identified by Q-TOF-MS/MS. kaempferol-3-O-rutinoside, narcissoside, and astragalin were first reported in GCP. respectively. The thirteen screened antioxidants were quantified simultaneously at their maximum absorption wavelengths, with concentrations ranging from 0.61 to 79.30 mg/g across three distinct flowering stages. Furthermore, the in vitro activity and molecular docking of oxidative stress-related enzyme xanthine oxidase (XOD) were utilized to evaluate the antioxidant capacity of the screened compounds. Consequently, our method effectively screens for antioxidants within complex mixtures, positioning GCP as a promising reservoir of natural bioactive compounds.
研究领域:
植物化学
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Yixin Huang,Jinbin Lin,Jing Zou,Jiaxin Xu,Mengyun Wang,Huahua Cai,Baoling Yuan,Jun Ma
Horseradish peroxidase (HRP)-catalyzed hydrogen peroxide (H 2 O 2 ) oxidation could degrade a variety of organic pollutants, but the intrinsic drawback of slow degradation rate limited its widespread application. In this study, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was introduced into HRP/H 2 O 2 system as an electron shuttle to enhance diclofenac degradation under neutral pH conditions. The green-colored ABTS radical (ABTS •+ ), generated by the oxidation of ABTS with HRP-catalyzed H 2 O 2 oxidation, was proved to be the main reactive species for the rapid degradation of diclofenac in HRP/H 2 O 2 /ABTS system. There was no destruction of ABTS/ABTS •+ in HRP/H 2 O 2 /ABTS system, and ABTS was verified as an ideal electron shuttle. The reaction conditions including solution pH (4.5–10.5), HRP concentration (0–8 units mL −1 ) and H 2 O 2 concentration (0–500 μM) would impact the formation of ABTS •+ , and affect the degradation of diclofenac in HRP/H 2 O 2 /ABTS system. Moreover, compared with Fenton and hydroxylamine/Fenton systems, HRP/H 2 O 2 /ABTS system had better diclofenac degradation efficiency, higher H 2 O 2 utilization efficiency and stronger anti-interference capacity in actual waters. Overall, the present study provided a meaningful and promising way to enhance the degradation of organic pollutants in water with HRP-catalyzed H 2 O 2 oxidation.
研究领域:
环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 26
·27063-27072
·
IF 2.914
Wang Mengyun,Wang Daiyao,Qiu Shiyi,Xiao Junyang,Cai Huahua,Zou Jing
In this study, a sensitive and low-cost multi-wavelength spectrophotometric method for the determination of hydrogen peroxide (H 2 O 2 ) in water was established. The method was based on the oxidative coloration of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) via Fenton reaction, which resulted in the formation of green radical (ABTS •+ ) with absorbance at four different wavelengths (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). Under the optimized conditions ( C ABTS = 2.0 mM, C Fe 2+ = 1.0 mM, pH = 2.60 ± 0.02, and reaction time ( t ) = 1 min), the absorbance of the generated ABTS •+ at 415 nm, 650 nm, 732 nm, and 820 nm were well linear with H 2 O 2 concentrations in the range of 0–40 μM ( R 2 > 0.999) and the sensitivities of the proposed Fenton-ABTS method were calculated as 4.19 × 10 4 M –1 cm –1 ,1.73 × 10 4 M –1 cm –1 , 2.18 × 10 4 M –1 cm –1 , and 1.96 × 10 4 M –1 cm –1 , respectively. Meanwhile, the detection limits of the Fenton-ABTS method at 415 nm, 650 nm, 732 nm, and 820 nm were respectively calculated to be 0.18 μM, 0.12 μM, 0.10 μM, and 0.11 μM. The absorbance of the generated ABTS •+ in ultrapure water, underground water, and reservoir water was quite stable within 30 min. Moreover, the proposed Fenton-ABTS method could be used for monitoring the variations of H 2 O 2 concentration during the oxidative decolorization of RhB in alkali-activated H 2 O 2 system.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Xin Liu,Qi Fu,Peng Xu,Pengfei Zhu,Zhuoyu Yang,John C. Crittenden
A sensitive spectrophotometric method (the Br − -ABTS method) for measuring monopersulfate (PMS) of 0–20 μM in water was established. The Br − -ABTS method was based on the oxidation coloration reaction where Br − as catalyst promoted PMS for oxidation of ABTS forming the green-colored ABTS radical (ABTS • + ) under pH 3.0 (50 mM phosphate buffer). ABTS • + could be quantitatively measured at 415 nm, 650 nm, 732 nm and 820 nm using UV–vis spectrophotometer. There was a well linear relationship between the absorbance of ABTS • + and PMS concentration with the value of R 2 being 0.999. The proposed Br − -ABTS method was highly sensitive, and the absorbance generated by Br − -ABTS at 415 nm was 6.59 × 10 4 cm −1 per M (mol L –1 ) of PMS. The changes of pH from 3.0 to 9.0 had an insignificant effect on the calibration curve of Br − -ABTS method. The reaction of PMS with ABTS had a stoichiometric coefficient of 1:1.96 at the pH 3.0. The residual absorbance of ABTS •+ in ultrapure water, Xiangjiang water and Hou-lake water was stable within 30 min. Moreover, the trace PMS concentration of 0.03–20 μM in ultrapure water, 0.05–20 μM in natural water (e. g. Xiangjiang water and Hou-lake water) and 1–20 μM in wastewater was accurately measured with the proposed Br − -ABTS method. Additionally, the interference of natural water matrix (e. g. humic acid), metal ions (e. g. Co(II), Fe(III) and Cu(II)) and hydroxylamine had barely interference for the proposed Br − -ABTS method. Furthermore, the Br − -ABTS method was more accurate and flexible to determine PMS concentration than DPD method during the decolorization of rhodamine B in the Co(II)/PMS system, Cu(II)/PMS system and hydroxylamine/Fe(III)/PMS process.
研究领域:
环境科学
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分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2020
·Vol. 58
·期 9
·875-879
·
IF 1.28
Qian Zheng-Ming,Fang Bo-Wen,Chen Hai-Ming,Li Chun-Hong,Huang Qi,Chen Ling,Li Wen-Jia,Li De-qiang
In the present study, an online liquid extraction coupled with high-performance liquid chromatography-2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (HPLC-ABTS) system for rapid screening of antioxidants in tea samples was proposed. As an example, the tea samples were firstly extracted by online HPLC extractor with mobile phase at 70°C, then the hyphenated HPLC-ABTS was used for the chromatographic separation on a Poroshell EC C18 column by 0.3% aqueous formic acid and acetonitrile with a gradient elution at 1.5 mL·min−1, and the UV and antioxidant chromatograms with detection wavelengths at 270 nm and 750 nm were recorded, respectively. The established system integrated the processes of online HPLC sample extraction, HPLC separation and online antioxidants detection, the total analysis time of which was <20 min. The developed method was successfully applied to samples of green tea, oolong tea and black tea. As a result, 11 antioxidants were found in tea samples, including gallocatechin, epigallocatechin, catechin, chlorogenic acid, epicatechin, epigallocatechingallate, epicatechingallate, rutin, 1,4,6-trigalloylglucose, quercetin-3-glycoside and kaempferol-3-glucoside. The combined online liquid microextraction and online HPLC-ABTS method is a rapid and green approach for the quality evaluation of tea.
研究领域:
食品科学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2021
·
IF 7.086
Jinbin Lin,Junyang Xiao,Hengyu Cai,Yixin Huang,Jiawen Li,Haoyu Yang,Tao Li,Jing Zou
In this study, a multi-wavelength spectrophotometric method for simultaneous determination of peracetic acid (PAA) and coexistent hydrogen peroxide (H 2 O 2 ) was presented. This method was based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) with the assistance of Fe 2+ /KI to produce a stable green radical (ABTS ●+ ), which could be determined at four characteristic peaks (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). The absorbances of ABTS ●+ at four peaks were well linear (R 2 > 0.999) with concentrations of both total peroxides (PAA + H 2 O 2 ) and PAA in the range of 0–40 μM under optimized conditions. The sensitivities for determining total peroxides at 415 nm, 650 nm, 732 nm and 820 nm were determined to be 4.248 × 10 4 M −1 cm −1 , 1.682 × 10 4 M −1 cm −1 , 2.132 × 10 4 M −1 cm −1 , and 1.928 × 10 4 M −1 cm −1 , respectively. For determining PAA, the corresponding sensitivities were 4.622 × 10 4 M −1 cm −1 , 1.895 × 10 4 M −1 cm −1 , 2.394 × 10 4 M −1 cm −1 and 2.153 × 10 4 M −1 cm −1 , respectively. The concentration of coexistent H 2 O 2 was gained by deducting PAA concentration from total peroxides concentration. The ABTS method was accurate enough to determine PAA concentration in natural water samples. Moreover, the ABTS method was successfully used to determine the changes of PAA and coexistent H 2 O 2 and to distinguish their role on naproxen degradation in heat-activated PAA process. Overall, the ABTS method could be used as an alternative method for the convenient, rapid and sensitive determination of PAA and the coexistent H 2 O 2 in water samples.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiang Qiu,Shushu Wang,Shanshan Miao,Hongbo Suo,Huajin Xu,Yi Hu
This work aims to achieve the co-immobilization of laccase and 2,2-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) to improve removal capability of the biocatalyst for pollutants while avoiding potential pollution caused by ABTS. The laccase was immobilized on magnetic chitosan nanoparticles modified with amino-functionalized ionic liquid containing ABTS (MACS-NIL) based on Cu ion chelation (MACS-NIL-Cu-lac). The carrier was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, x-ray diffraction and etc., and electron paramagnetic resonance confirmed the mediator molecule ABTS on the carrier could also play the role of electron transmission. MACS-NIL-Cu-lac presented relatively high immobilization capacity, enhanced activity (1.7-fold that of free laccase), improved pH and temperature adaptability, and increased thermal and storage stability. The removal performance assay found that MACS-NIL-Cu-lac had a good removal efficiency with 100.0 % for 2,4-dichlorophenol in water at 25 °C, even when the concentration reached 50 mg/L. Reusability study showed that after six catalytic runs, the removal efficiency of 2,4-dichlorophenol by MACS-NIL-Cu-lac could still reach 93.2 %. Additionally, MACS-NIL-Cu-lac exhibited higher catalytic efficiencies with 100.0 %, 70.5 % and 93.3 % for bisphenol A, indole, and anthracene, respectively. The high catalytic performance in pure water system obtained by the novel biocatalyst co-immobilizing laccase and electron mediator ABTS showed greater practical application value.
研究领域:
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2387-2394
·
IF 4.58
Tang Ying,Lai Wenqiang,Zhang Jin,Tang Dianping
This work reports on a sensitive colorimetric immunoassay for aflatoxin B 1 (AFB 1 ) in foodstuff. The reagent L-ascorbic acid 2-phosphate (AAP) is added to the system, and alkaline phosphatase (ALP) hydrolyzes AAP under formation of ascorbic acid and phosphate. The ascorbic acid produced reduces chloroauric acid to form zero-valent gold (in the form of nanoparticles). Hence, Au(III) is no longer available to oxidize 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) to form a green product. Rather, the solution remains colorless. By using ALP-labelled monoclonal anti-AFB 1 antibody, a competitive enzyme-label immunoassay was developed for AFB 1 in a microplate coated with the AFB 1 -BSA conjugate. Under optimal conditions, the absorbance of the solution at 415 nm increases linearly with increasing AFB 1 concentration in range from 10 pg·mL −1 to 100 ng·mL −1 (while the color gradually turns to green), and the detection limit is 7.8 pg·mL −1 . The precision of the method (expressed as RSD) is ±9.7%. The accuracy was validated by analyzing both naturally contaminated and spiked food samples, and the results matched the results obtained by ELISA very well.
Schematic of a colorimetric aflatoxin B 1 immunoassay. Antibody is labeled with alkaline phosphatase which catalyzes the hydrolysis of ascorbic acid 2-phosphate (AAP) to form ascorbic acid which reduces chloroauric acid to form gold nanoparticles. Hence, Au(III) is no longer available to oxidize 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) to form a green product.
研究领域:
食品科学
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）