Evidence-based Complementary and Alternative Medicine
·
2023
·
IF 2.65
Wei-Qi Yang,Zheng-Ming Qian,Meng-Qi Wu,Jian-Li Gao,Qi Huang,Yuan-Sheng Zou,Dan Tang
The root of Polygonum bistorta (PB) is a traditional Chinese medicinal plant material widely used in China. It has been commonly used for the treatment of hemostasis, detumescence, diarrhea, snake bite, and acute gastroenteritis. However, the research on the antioxidant properties and bioactive compounds from PB is inadequate. In the current research, an online microextraction (OLME) coupled with a high-performance liquid chromatography coupled with the 2,2-nitrogen-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt antioxidant assay (HPLC-ABTS) system for rapid analysis of antioxidants from PB was proposed. The PB sample (0.17 mg) was online extracted by mobile phase (acetonitrile and 0.2% acetic acid); a Poroshell 120 SB-Aq column was used for separation; then, an online ABTS assay system was used for screening the antioxidants. Finally, ten components were found in PB, and among them, eight components possessed antioxidant activities. Furthermore, five components (gallic acid, neochlorogenic acid, caffeic acid, chlorogenic acid, and an unknown compound) were proved as major antioxidants when compared with rutin as an antioxidant marker. The results showed that the developed OLME-HPLC-ABTS system was a simple, rapid, green, and efficient instrument for the screening of antioxidants from PB, which provides a powerful tool for the discovery of natural antioxidants in Chinese medicines.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Shen Peng,Yuqin Wang,Junjun Yang,Lei Wang
A new method for the quantification of trace periodate (PI) concentration was developed. The Br - -ABTS method was based on the oxidation coloration reaction. Under Br - catalysis, the PI rapidly oxidized ABTS to the green ABTS •+ , which could be accurately determined by UV-Vis spectrophotometer. The absorbance of ABTS •+ was remarkably correlated linearly with the concentration of PI at 415 nm, and the slope of standard curve was (3.77 ± 0.01)× 10 4 M −1 ·cm −1 . The proposed method had high sensitivity and the slope at different optical paths showed a good multiplicative relationship for concentration values as low as 0.02 μM to be measured. The existence of different solutes in river and lake water did not affect the accuracy of Br - -ABTS method, the generated ABTS •+ could maintain good stability within 30 min, and the recovery of PI was between 95%− 105%. The stoichiometric coefficient between PI and ABTS •+ was 1.12 at pH 3.0. In addition, a common reductant (hydroxylamine), transition metal ions and inorganic anions (Cl - , SO 4 2- and HCO 3 - ) did not affect the Br - -catalyzed oxidation of ABTS for the measurement of PI concentration. Consequently, Br - -ABTS is a feasible method to determine the concentration of PI.
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Jing Zou,Yixin Huang,Lirong Zhu,Zheng Cui,Baoling Yuan
A spectrophotometric method using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as the indicator was proposed for measuring persulfates, i.e., peroxymonosulfate (PMS) and peroxydisulfate (PDS). This method was based on the direct oxidation of the colorless ABTS by PMS/PDS to generate the green-colored ABTS + at neutral pH and room temperature . The generated ABTS + had four different characteristic absorption peaks at 820 nm, 732 nm, 650 nm and 415 nm. The absorption values at these wavelengths increased with the increase of PMS/PDS concentration, and there were well linear relationships (R 2 > 0.998) between the corresponding absorption values and the concentrations of PMS/PDS in the range of 3–500 μM. Monitoring the change of PMS/PDS concentration by the proposed ABTS method was in good agreement with that monitored by the common spectrophotometric method using potassium iodide as the indicator. The green-colored waste solutions produced after PMS/PDS analysis with the ABTS method showed no bactericidal effect towards Escherichia coli . The proposed multi-wavelength spectrophotometric method using ABTS as the indicator could be employed as a candidate method for the accurate and convenient measurement of PMS/PDS in water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 26
·27063-27072
·
IF 2.914
Wang Mengyun,Wang Daiyao,Qiu Shiyi,Xiao Junyang,Cai Huahua,Zou Jing
In this study, a sensitive and low-cost multi-wavelength spectrophotometric method for the determination of hydrogen peroxide (H 2 O 2 ) in water was established. The method was based on the oxidative coloration of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) via Fenton reaction, which resulted in the formation of green radical (ABTS •+ ) with absorbance at four different wavelengths (i.e., 415 nm, 650 nm, 732 nm, and 820 nm). Under the optimized conditions ( C ABTS = 2.0 mM, C Fe 2+ = 1.0 mM, pH = 2.60 ± 0.02, and reaction time ( t ) = 1 min), the absorbance of the generated ABTS •+ at 415 nm, 650 nm, 732 nm, and 820 nm were well linear with H 2 O 2 concentrations in the range of 0–40 μM ( R 2 > 0.999) and the sensitivities of the proposed Fenton-ABTS method were calculated as 4.19 × 10 4 M –1 cm –1 ,1.73 × 10 4 M –1 cm –1 , 2.18 × 10 4 M –1 cm –1 , and 1.96 × 10 4 M –1 cm –1 , respectively. Meanwhile, the detection limits of the Fenton-ABTS method at 415 nm, 650 nm, 732 nm, and 820 nm were respectively calculated to be 0.18 μM, 0.12 μM, 0.10 μM, and 0.11 μM. The absorbance of the generated ABTS •+ in ultrapure water, underground water, and reservoir water was quite stable within 30 min. Moreover, the proposed Fenton-ABTS method could be used for monitoring the variations of H 2 O 2 concentration during the oxidative decolorization of RhB in alkali-activated H 2 O 2 system.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2387-2394
·
IF 4.58
Tang Ying,Lai Wenqiang,Zhang Jin,Tang Dianping
This work reports on a sensitive colorimetric immunoassay for aflatoxin B 1 (AFB 1 ) in foodstuff. The reagent L-ascorbic acid 2-phosphate (AAP) is added to the system, and alkaline phosphatase (ALP) hydrolyzes AAP under formation of ascorbic acid and phosphate. The ascorbic acid produced reduces chloroauric acid to form zero-valent gold (in the form of nanoparticles). Hence, Au(III) is no longer available to oxidize 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) to form a green product. Rather, the solution remains colorless. By using ALP-labelled monoclonal anti-AFB 1 antibody, a competitive enzyme-label immunoassay was developed for AFB 1 in a microplate coated with the AFB 1 -BSA conjugate. Under optimal conditions, the absorbance of the solution at 415 nm increases linearly with increasing AFB 1 concentration in range from 10 pg·mL −1 to 100 ng·mL −1 (while the color gradually turns to green), and the detection limit is 7.8 pg·mL −1 . The precision of the method (expressed as RSD) is ±9.7%. The accuracy was validated by analyzing both naturally contaminated and spiked food samples, and the results matched the results obtained by ELISA very well.
Schematic of a colorimetric aflatoxin B 1 immunoassay. Antibody is labeled with alkaline phosphatase which catalyzes the hydrolysis of ascorbic acid 2-phosphate (AAP) to form ascorbic acid which reduces chloroauric acid to form gold nanoparticles. Hence, Au(III) is no longer available to oxidize 2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) to form a green product.
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2024
·
IF 5.3
Qinhai Xu,Kang Li,Peng Wang
The reaction mechanism of p -hydroxystyryl-substituted BODIPY (BOH) with two oxidative cations, namely ABTS •+ and Fe 3+ , was investigated in a water–ethanol mixed solution and in liposome suspensions, respectively, using different spectroscopic methods. In solution, the oxidation of BOH (with orange fluorescence) by the two cations occurred at the ethylene group (C C) locating between the dipyrrole and phenol groups and resulted in conjugation-truncated products exhibiting characteristic green fluorescence emission. In heterogeneous small unilamellar vesicles (SUV), water soluble ABTS •+ was evidenced to oxidize BOH embedded in the lipid bilayers of SUV, while Fe 3+ did not. The lack of reaction between Fe 3+ and BOH was attributed to the complexation between Fe 3+ and the phenolic hydroxyl group of BOH on the surface of the SUV. The reaction kinetics results indicated that, in homogeneous solution, the oxidation rate of Fe 3+ was three orders of magnitude slower than that of ABTS •+ for BOH. The location and orientation of BOH within the SUV were discussed based on the reaction phenomena. BOH could be as a good antioxidant fluorescent prober for ABTS •+ detection with a detection limit of 1.5 * 10 −7 M and a linear rang of 0–4.93 μM. What’s more, the amphiphilic BOH dispersed in the round GUV (BOH + GUV) could show the bright red fluorescence. This research suggests the significant potential of BOH as an antioxidant fluorescent probe for in situ bioimaging.
✓ 已验证
产品关联
DOI
高影响因子
Chemical Engineering Journal Advances
·
2022
·
IF 0
Na Yuan,Hongchao Li,Jieshu Qian
Determination of the concentration of peracetic acid (PAA) in the presence of H 2 O 2 is a great challenging problem due to the similar chemical behaviors of the two species. In this study, we developed a new method for simultaneous measurement of PAA (0.5 – 10.0 μM) and total peroxides (i.e., PAA and H 2 O 2 with a molar ratio of 10:6.5), based on the catalytic oxidation of ABTS by separately using Co(II) and peroxidase (POD) as the catalysts. Owing to the unique catalytic activity of Co(II) towards PAA, the coexistent H 2 O 2 exhibits negligible effect on the determination of PAA. The value of absorbance at 415 nm increases linearly with increasing the concentration of PAA or total peroxides, and the sensitivities for PAA and total peroxides are as high as 5.31 × 10 4 and 9.07 × 10 4 M −1 cm −1 , respectively. In addition, the proposed ABTS method shows excellent feasibility and applicability, which make it suitable to be employed for detection in complicated water matrices.
✓ 已验证
DOI
ACS ES&T Water
·
2025
·
IF 4.3
Shule Duan,Zhimin Qiang,Yu Lei,Shaogang Liu,Ting Xie,Zhenyu Wang,Huiyu Dong
Spectrophotometry can efficiently determine permanganate (PM)-based chemical oxygen demand (CODMn) in various waters, but existing methods face challenges, such as large reagent requirements and interference from complex water matrices. Here, we developed a novel spectrophotometric method for determining the CODMn using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) as an indicator. The kinetics and thermodynamics of the PM + ABTS reaction were investigated, revealing a pseudo-first-order rate constant (k′) of 36.4–48.6 s–1 at [PM]0:[ABTS]0 molar ratios of 1:60–1:100 (pH 6.5 and 298 K) and an Arrhenius activation energy (Ea) of 17.5 kJ mol–1. Compared to the PM + DPD (N,N-diethyl-p-phenylenediamine) reaction, which has a k′ of 29.4–47.5 s–1 under identical conditions and an Ea of 47.4 kJ mol–1, the PM + ABTS reaction demonstrated greater adaptability for rapid CODMn determination. Furthermore, the ABTS method showed higher sensitivity and accuracy for CODMn determination than the DPD method, with a higher slope of the linear calibration curve (0.228 L mg–1 cm–1 vs 0.101 L mg–1 cm–1) and a lower limit of quantification (0.03 mg L–1 vs 0.05 mg L–1). In addition, both methods showed high robustness against different water matrices, including real waters. Although the ABTS method has a higher reagent cost than the DPD method, it offers sensitive, rapid, and accurate CODMn determination, making it suitable for time-sensitive water quality assessments.
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yixin Huang,Jinbin Lin,Jing Zou,Jiaxin Xu,Mengyun Wang,Huahua Cai,Baoling Yuan,Jun Ma
Horseradish peroxidase (HRP)-catalyzed hydrogen peroxide (H 2 O 2 ) oxidation could degrade a variety of organic pollutants, but the intrinsic drawback of slow degradation rate limited its widespread application. In this study, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) was introduced into HRP/H 2 O 2 system as an electron shuttle to enhance diclofenac degradation under neutral pH conditions. The green-colored ABTS radical (ABTS •+ ), generated by the oxidation of ABTS with HRP-catalyzed H 2 O 2 oxidation, was proved to be the main reactive species for the rapid degradation of diclofenac in HRP/H 2 O 2 /ABTS system. There was no destruction of ABTS/ABTS •+ in HRP/H 2 O 2 /ABTS system, and ABTS was verified as an ideal electron shuttle. The reaction conditions including solution pH (4.5–10.5), HRP concentration (0–8 units mL −1 ) and H 2 O 2 concentration (0–500 μM) would impact the formation of ABTS •+ , and affect the degradation of diclofenac in HRP/H 2 O 2 /ABTS system. Moreover, compared with Fenton and hydroxylamine/Fenton systems, HRP/H 2 O 2 /ABTS system had better diclofenac degradation efficiency, higher H 2 O 2 utilization efficiency and stronger anti-interference capacity in actual waters. Overall, the present study provided a meaningful and promising way to enhance the degradation of organic pollutants in water with HRP-catalyzed H 2 O 2 oxidation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 19
·7973-7977
·
IF 1.938
Yingfeng Qin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
A homogeneous label-free colorimetric sensing strategy for bleomycin (BLM) assay was developed on the basis of BLM enhanced Fe(II)–H2O2–ABTS reaction. Fe(II) exhibits a catalytic effect on H2O2-mediated oxidation of ABTS by Fenton chemistry. BLM·Fe(II) complex possessed a significantly higher catalytic ability than Fe(II) probably because of the further formation of more active “activated oxo-iron-bleomycin complex”. The present method was convenient without any complicated chemical synthesis, modification, or tedious experimental procedures. Analysis results can be seen with the naked eye and monitored by UV-vis spectra. The peak absorbance exhibits a linear relationship with BLM concentrations from 25 nM to 1 μM, and a detection limit of 16 nM can be achieved. The designed colorimetric strategy may provide a promising alternative for BLM detection in clinical samples.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xiang Qiu,Shushu Wang,Shanshan Miao,Hongbo Suo,Huajin Xu,Yi Hu
This work aims to achieve the co-immobilization of laccase and 2,2-binamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) to improve removal capability of the biocatalyst for pollutants while avoiding potential pollution caused by ABTS. The laccase was immobilized on magnetic chitosan nanoparticles modified with amino-functionalized ionic liquid containing ABTS (MACS-NIL) based on Cu ion chelation (MACS-NIL-Cu-lac). The carrier was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, x-ray diffraction and etc., and electron paramagnetic resonance confirmed the mediator molecule ABTS on the carrier could also play the role of electron transmission. MACS-NIL-Cu-lac presented relatively high immobilization capacity, enhanced activity (1.7-fold that of free laccase), improved pH and temperature adaptability, and increased thermal and storage stability. The removal performance assay found that MACS-NIL-Cu-lac had a good removal efficiency with 100.0 % for 2,4-dichlorophenol in water at 25 °C, even when the concentration reached 50 mg/L. Reusability study showed that after six catalytic runs, the removal efficiency of 2,4-dichlorophenol by MACS-NIL-Cu-lac could still reach 93.2 %. Additionally, MACS-NIL-Cu-lac exhibited higher catalytic efficiencies with 100.0 %, 70.5 % and 93.3 % for bisphenol A, indole, and anthracene, respectively. The high catalytic performance in pure water system obtained by the novel biocatalyst co-immobilizing laccase and electron mediator ABTS showed greater practical application value.
✓ 已验证
DOI
高影响因子
MOLECULES
·
2017
·Vol. 22
·期 6
·920
·
IF 2.861
Youxun Liu,Yuanyuan Geng,Mingyang Yan,Juan Huang
The successful encapsulation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules. ABTS@MIL-100(Fe) was then evaluated as an immobilized laccase mediator for dye removal using indigo carmine (IC) as a model dye. Through the application of laccase in combination with a free (ABTS) or immobilized (ABTS@MIL-100(Fe)) mediator, decolorization yields of 95% and 94%, respectively, were obtained for IC after 50 min. In addition, following seven reuse cycles of ABTS@MIL-100(Fe) for dye treatment, a decolorization yield of 74% was obtained. Dye decolorization occurred through the breakdown of the chromophoric group by the Laccase/ABTS@MIL-100(Fe) system, and a catalytic mechanism was proposed. We therefore expect that the stability, reusability, and validity of ABTS@MIL-100(Fe) as a laccase mediator potentially render it a promising tool for dye removal, in addition to reducing the high running costs and potential toxicity associated with synthetic mediators.Keywords:laccase;ABTS;metal-organic framework;mediator;decolorization
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）