Nano Materials Science
·
2025
·
IF 17.9
Yaqing Zhou,Zunquan Zhao,Yang Cao,Yaqing Zhao,Aimei Zhang,Ailing Zhang,Jialei Bai,Yuan Peng
Covalent organic framework (COF) materials have the advantages of good chemical stability, regular pores, high specific surface area, and it is widely used in adsorption, catalysis, and biosensors. However, the construction of photonic crystal (PC) with COF remains a challenge. In this work, we developed a new type of COF PC, which had an inverse opal array structure, called covalent organic framework - inverse opal photonic crystal (COF-IOPC). The COF-IOPC was prepared by sacrificial template method, which was simple and rapid. The SiO 2 microspheres was used to prepare the opal template through the restricted meniscus self-assembly method, and then, the COF pre-reaction solution was added to fulfill the crevices of the opal template. By controlling the size of the SiO 2 particles of the opal template, the photonic band gap of the COF-IOPC could be manipulated to exhibit different structural colors. Furthermore, the COF-IOPC could be used to detect Aflatoxin B 1 (AFB 1 ) without label. The spiked real samples were detected through the national standard method Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS) as comparison, and the results indicated that COF-IOPC sensing method had a well accuracy with the LC-MS/MS method. The successful construction of this novel material further expands the application field of COF material.
研究领域:
材料科学
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分析化学
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生物传感器
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纳米技术
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DOI
高影响因子
Analytical Methods
·
2022
·Vol. 15
·期 1
·70-78
·
IF 3.532
Hongyan Zhan,Si Yang,Chenxi Li,Rong Liu,Wenliang Chen,Xiaoli Wang,Yansong Zhao,Kexin Xu
Aflatoxin B1 (AFB1) is a typical mycotoxin found in agricultural products, and poses a huge threat to both humans and animals. Accurate and rapid measurement of AFB1 is essential for environmental analysis and food safety. Based on molecular docking simulation design and exonuclease-assisted target recycling amplification, we designed a competitive fluorescence aptasensor to detect AFB1 rapidly and sensitively. According to the molecular docking simulations, a complementary strand (cDNA) was designed by searching for potential binding sites of the aptamer, which had the lowest binding energy. Magnetic beads modified with biotin-Apt were used as the capture probe, while FAM-labeled cDNA acted as the reporter probe. By using EXO I for target recycling amplification, this aptasensor was highly sensitive and selective for AFB1. The detection limit of the suggested aptasensor under optimal conditions was 0.36 ng mL−1 (S/N = 3) in the range of 1–1000 ng mL−1 (R2 = 0.991). The developed aptasensor was successfully used to analyze AFB1 in oil samples.
研究领域:
食品安全
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环境分析
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生物技术
·
分析化学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Mingsan Miao,Liang Guo,Jinyan Xue,Yuzhen Jia,Zhenzhen Cui,Huai-Xia Yang
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. In this sensing system, AFB1 and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, which were used as recognition probes to specifically capture the target. Activators generated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as signal amplification strategy to improve the sensitivity. The initiator modified at the end of the antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve simultaneous detection of OTA and AFB1 with the limit of 18.62 and 1.60 fg/mL for AFB1 and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
研究领域:
分析化学
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生物技术
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食品科学
·
毒理学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2019
·
IF 4.527
Sihong Li,Ishfaq Muhammad,Hongxiao Yu,Xiaoqi Sun,Xiuying Zhang
In this study, we identified AFB1 adducts as potential markers and investigated the role of curcumin in alleviating AFB1-induced liver damage by suppressing the production of AFB1 adducts and oxidative stress in AA broilers liver. A total of 64 one-day-old Arbor Acres (AA) broilers were randomly divided into four groups, including control group, AFB1 group (5 mg/kg AFB1), cur + AFB1 group (300 mg/kg curcumin+5 mg/kg AFB1) and curcumin group (300 mg/kg). Serum biochemical parameters, liver antioxidant abilities, AFB1 adducts and oxidative stress mechanism were studied in broilers. AFB1 administration accompany with signs of liver injury, including hepatic histological lesions, increased serum enzymes activities , decreased liver antioxidant enzymes activities and the suppression of ROS and 8-OHdG. Meanwhile, Nrf2/HO-1 pathway was depressed by AFB1 treatment. Immunohistochemistry and ELISA showed that AFB1 significantly increased AFB1-DNA adduct in liver ( p < 0.05) and AFB1-lysine adduct in serum ( p < 0.05). Importantly, supplementation of curcumin can ameliorate these alterations. Intriguingly, curcumin alleviated AFB1-induced toxicity and oxidative stress by inhibiting the generation of ROS, 8-OHdG and AFB1 adducts, and activated Nrf2 signaling pathway in broilers. Conclusively, our experiments suggest that curcumin could be considered as a potential agent for prevention of AFB1-induced toxicity and oxidative stress, and AFB1 adducts could be suitable therapeutic targets.
研究领域:
兽医学
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毒理学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying Li,Boyu Jia,Pengyue Song,Nan Long,Linchun Shi,Peng Li,Jiabo Wang,Lidong Zhou,Weijun Kong
As nucleic acid-based affinity elements, aptamers have attracted significant attention for a wide range of analytical applications. Although several aflatoxin B 1 (AFB 1 ) aptamers have been identified, they are unsuitable for overcoming the unavoidable cross-reactions from interferents in complex food matrices due to their poor binding affinities and specificities. Herein, a novel precision-systematic evolution of ligands by exponential enrichment (P-SELEX) strategy through introducing the counter (matrix without target AFB 1 ) and positive (with AFB 1 ) screening steps was implemented to accurately identify AFB 1 aptamers. A DNA aptamer A-42-2 at a 24-nt length was selected finally, which possessed nanomolar-level affinity of 5.55 nM, high specificity to other interferents, and strong anti-cross-reactivity ability for matrix components. Then, an A-42-2 aptamer-based ultra-sensitive colorimetric and fluorescent dual-readout aptasensor was fabricated for AFB 1 detection in three kinds of complex food samples rich in starch without cross-reactions. The aptasensor displayed outstanding detection capacity with a wide liner range of 0.25–30 nM (1.95–234.4 μg/kg), while the detection limit for colorimetric measurement as low as 0.22 nM (1.72 μg/kg) and 0.048 nM (0.20 μg/kg) for fluorescent determination. P-SELEX is ideal for screening and applying aptamers in complex food matrices, creating more opportunities for the efficient and cost-effective development of high-quality aptamers and aptasensors for other targets.
研究领域:
分析化学
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食品科学
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生物技术
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhihui Wu,Da-Wen Sun,Hongbin Pu,Qingyi Wei,Xuanran Lin
Mycotoxin B1 (AFB1) contamination in agricultural products pose a deadly danger to animal and human health and its rapid and reliable detection is thus very important. Herein, a ratiometric surface-enhanced Raman scattering (SERS) aptasensor for AFB1 detection was developed, in which 1,2-bis(4-pyridyl) ethylene (BPE) was used to trigger the assembly of Au nanoparticle dimers (AuNP dimers) and form intensive SERS “hot spots”, and MXenes nanosheets could load aptamer-modified AuNP dimers due to the hydrogen bonding and the chelation between the phosphate groups of aptamers and the Ti ion of MXenes. With the presence of AFB1 preferentially binding to AFB1 aptamer, AuNP dimers were separated from MXenes nanosheets, leading to a decrease in SERS intensity. Regression analysis in the range from 0.001 to 100 ng·mL −1 showed the limit of detection (LOD) being 0.6 pg·mL −1 in standard solution, indicating that the great prospects of the AuNP dimers/MXenes SERS substrate for detecting AFB1.
研究领域:
分析化学
·
纳米技术
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食品安全
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Haoyang He,Da-Wen Sun,Hongbin Pu,Zhihui Wu
Food aflatoxin B1 (AFB1) contamination greatly threatens human health and its sensitive determination is imperative. In this study, a surface-enhanced Raman scattering (SERS) and fluorescence dual-signal aptasensor was constructed for sensitive AFB1 detection in peanuts, walnuts, and almonds samples. Fluorescent dye cy5 was used as fluorophore and Raman reporter, while polyethyleneimine modified Ag coating magnetic nanoparticles (MNP@Ag-PEI) were utilized to absorb the cy5 modified aptamer (apt-cy5). Results indicated that linear ranges of 0.001–1000 ng/mL and 0.2–20,000 ng/mL with detection limits of 0.45 pg/mL and 0.135 ng/mL for the SERS and fluorescence methods were obtained, respectively, and AFB1 detection in the nut samples using the aptasensor achieved satisfactory recoveries of 95.2%–108.6% for SERS and 94.7%–109.7% for fluorescence. Compared with other mono signal detection, the established aptasensor facilely fused the merits of the two signals and improved the detection accuracy and flexibility.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 187
·期 1
·1-8
·
IF 5.479
Liang Yutao,He Juan,Huang Zhipeng,Li Huayu,Zhang Yunxia,Wang Huige,Rui Chaofan,Li Yuanyuan,You Liqin,Li Kai,Zhang Shusheng
A surface imprinted polymer of type UiO-66-NH2@MIP was prepared by combining molecular imprinted polymers (MIPs) and an amino-functionalized zirconium-based metal-organic framework. Quercetin is used as the virtual template, UiO-66-NH 2 acts as the carrier to which the monomer acrylamide can be copolymerized. The material was characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. It was used as a sorbent in a solid-phase extraction column. The extraction conditions were optimized. The adsorption capacities for aflatoxins AFB1, AFB2, AFG1 and AFG2 by this SPE and by the commercial SPE were compared. The method was successfully applied to quantify the aflatoxins in grain. Figures of merit include (a) good linearity (range from 0.20–45 μg·kg −1 ) with R 2 (range from 0.9986–0.9994), (b) low detection limits (90–130 ng·kg −1 ), (c) acceptable reproducibility (1.0–5.9%; for n = 6), and (d) relatively satisfactory recovery rates (74.3–98.6%). The new sorbent has good selectivity and reusability.
UiO-66-NH 2 @MIPs were synthesized with modified UIO-66-NH 2 as core and quercetin as pseudo template. A cartridge was prepared with the polymers as the sorbent, and its performance was compared with different commercial SPE cartridges.
研究领域:
分析化学
·
食品科学
·
材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 6
·1-10
·
IF 6.232
Liu Xin,Wen Yangping,Wang Wenjun,Zhao Zitong,Han Yi,Tang Kaijie,Wang Dan
A novel nanobody (Nb)-based voltammetric immunosensor coupled with horseradish peroxidase concatemer–modified hybridization chain reaction (HRP-HCR) signal amplifying system is described to realize the rapid and ultrasensitive detection of AFB 1 . To design such an immunoassay, anti-AFB 1 Nbs with smaller molecular size were coated densely onto the surface of Au nanoparticle-tungsten disulfide-multi-walled carbon nanotubes (AuNPs/WS 2 /MWCNTs) functional nanocomposites as an effective molecular recognition element, whereas AFB 1 -streptavidin (AFB 1 -SA) conjugates were ingeniously bound with biotinylated HCR dsDNA nanostructures as the competitor, amplifier, and signal report element. In the presence of AFB 1 targets, a competitive immunoreaction was performed between the analyte and AFB 1 -SA-labeled HCR (AFB 1 -HCR) platform. Upon the addition of SA-modified polyHRP (SA-polyHRP), AFB 1 -HCR nanostructures containing abundant biotins were allowed to cross-link to a quantity of HRP by streptavidin−biotin chemistry for signal amplification and signal conversion. Under optimal conditions, the immunosensor displayed a good linear correlation toward AFB 1 ranging from 0.5 to 10 ng mL −1 with a sensitivity of 2.7 μA (mL ng −1 ) and an ultralow limit of detection (LOD) of 68 fg mL −1 . The specificity test showed that the AFB 1 immunosensor had no obvious cross-reaction with OTA, DON, ZEN, and FB 1 . The signal of this sensor decreased by 10.18% in 4 weeks indicating satisfactory stability, and its intra- and inter-laboratory reproducibility was 3.42~10.35% and 4.03%~12.11%, respectively. This biosensing system will open up new opportunities for the detection of AFB 1 in food safety and environmental analysis and extend a wide range of applications in the analysis of other small molecules.
Graphical abstract
研究领域:
食品安全
·
环境分析
·
生物传感技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
·
IF 5.3
Sun Danni,Liu Tao,Yao Yiran,Kong Dezhao,Liu Chang,Ye Hua,Zhang Qi,Li Shijie,Li Yaqi,Shi Qiaoqiao
A surface-enhanced Raman scattering (SERS) aptasensor was developed using gold nanostars (Au NSs) and Fe 3 O 4 @Au nanoparticles (NPs) for the highly sensitive detection of aflatoxin B 1 (AFB 1 ). Au NSs were modified by the Raman reporter 4-aminothiophenol (PATP) and then coupled with cDNA to act as the capture probes (Au NSs@PATP-cDNA). Fe 3 O 4 @Au NPs were modified with the AFB 1 aptamer (AFB 1 Apt) and used as signal probes (Fe 3 O 4 @Au NPs-AFB 1 Apt). The SERS peak of PATP at 1078 cm −1 was used for quantitative analysis. When the core-satellite nanostructures (Fe 3 O 4 @Au NPs-AFB 1 Apt/cDNA-Au NSs@PATP) were self-assembled by oligonucleotide hybridization, the SERS intensity was significantly enhanced. When AFB 1 was present, AFB 1 Apt specifically binds to AFB 1 , causing the Fe 3 O 4 @Au NPs-AFB 1 Apt and Au NSs@PATP-cDNA to dissociate, resulting in a decrease in the SERS intensity measured after magnetic separation. Under optimal conditions, the limit of detection (LOD) of AFB 1 can be reduced to 0.24 pg/mL. This is attributed to the high affinity of AFB 1 Apt, excellent magnetic separation characteristics, and multiple SERS hotspots. The assay has been validated to perform well in recovery and accuracy by evaluating spiked samples (rice, corn, and wheat) and positive samples (corn, brown rice, and wheat). Graphical
研究领域:
分析化学
·
食品安全
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Wei Xu,Yonghua Xiong,Weihua Lai,Yang Xu,Changming Li,Mingyong Xie
Mycotoxins are fatal threats in food safety due to their strong carcinogenesis and toxicity, thus requiring highly sensitive detections . Herein, different-sized quantum dots (QDs) were used to construct a Förster resonance energy transfer (FRET) based immunosensor for sensitive detection of aflatoxin B 1 (AFB 1 ) in rice grains. To avoid irregular aggregation between two kinds of QDs, monovalent monoclonal antibody (mAb)-labeled red QDs (~0.84 anti-AFB 1 mAbs per QD) and multivalent hapten-labeled green QDs (~6.8 AFB 1 per QD) were designed as acceptor and donor, respectively. The anti-AFB 1 mAbs and AFB 1 interactions promoted one or more acceptors bound with a multivalent AFB 1 -labeled donor, resulting in energy transfer from the green QDs to the red QDs. Various parameters that influence the immunoassay including reactant ratio of donor to acceptor, buffer pH value, buffer ionic strength and immunoreaction time were systematically investigated and optimized. With optimal conditions, the obtained energy transfer efficiency is proportional to the logarithm of AFB 1 concentration in a range over 0.19–16 pM (0.06–5 ng/mL), while offering a limit of detection of 0.13 pM (0.04 ng/mL) in rice extracts. The recovery rates of the intra-assay for spiked samples at AFB 1 concentrations of 0.1, 1.0, and 5.0 ng/mL were 83.27%±3.27%, 97.36%±4.55% and 83.04%±4.94%, respectively, and those for the inter-assay were 81.28%±6.11%, 95.97%±7.07%, and 82.78%±5.99%, respectively. Statistical analysis using t -test had no significant difference between the proposed FRET-based immunoassay and the commercial enzyme-linked immunosorbent assay kit.
研究领域:
食品安全
·
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Le Wang,Pengxue Sun,Mingna Li,Yan Shen,Yangyong Lv,Hailong Tian,Dapeng Wu,Ying Liang,Qipeng Yuan,Jinshui Wang
A novel biological detoxification polymer (BDP) was in-situ synthesis by the co-immobilization of the compound enzymes (laccase and Aspergillus niger crude enzyme) and the aminated mesoporous silica nanoparticles (MSN-NH 2 ) with zeolitic imidazolate framework-8 (ZIF-8) for the detoxification of aflatoxin B 1 (AFB 1 ). The ZIF-8 shell of BDP acted as the AFB 1 adsorbent around the compound enzymes inside the BDP. The detoxification rate of BDP at 35 °C for 24 h was 96.7%. The detoxification of AFB 1 with the added mass ratio of BDP 1% to moldy corn was 65.8%. After the BDP was stored at 4 °C for 30 d, the enzyme activity remained 70.9%. Moreover, the enzyme activity and detoxification rate with BDP remained 80.7% and 80.0%, respectively, after the fourth repeated detoxification. The compound enzymes acted on the C–O bond and methoxyl group in the side chain of AFB 1 . Three degradation products (C 16 H 16 O 5 , C 16 H 14 O 4 , C 15 H 14 O 2 ) with different structures were detected in the degradation of AFB 1 with the BDP. Application of the in-situ BDP represents a promising way to improve the detoxification of AFB 1 .
研究领域:
食品科学
·
生物技术
·
环境科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）