Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 21
·4031-4039
·
IF 7.571
Xu Li,Kang Cui,Mingzhen Xiu,Chenxi Zhou,Li Li,Jing Zhang,Shiji Hao,Lina Zhang,Shenguang Ge,Yizhong Huang,Jinghua Yu
In this work, novel dual-mode lab-on-paper devices based on in situ grown WO3/BiVO4 heterojunctions onto cellulose fibers, as signal amplification probes, were successfully fabricated by the integration of photoelectrochemical (PEC)/colorimetric analysis technologies into a paper sensing platform for the ultrasensitive detection of alpha-fetoprotein (AFP). Specifically, to achieve an impressive PEC performance of the lab-on-paper device, the WO3/BiVO4 heterojunction was in situ grown onto the surface of cellulose fibers assisted with Au nanoparticle (Au NP) functionalization for enhancing the conductivity of the working zone of the device. With the target concentration increased, more immune conjugates could be captured by the proposed paper photoelectrode, which could lead to a quantitative decrease in the photocurrent intensity, eventually realizing the accurate PEC signal readout. To meet the requirement of end-user application, a colorimetric signal readout system was designed for the lab-on-paper device based on the color reaction of 3,3′5,5′-tetramethylbenzidine (TMB) oxidized by WO3/BiVO4 nanoflowers in the presence of H2O2. Noticeably, it is the first time that the WO3/BiVO4 heterojunction is in situ grown onto cellulose fibers, which enhances the sensitivity in view of both their PEC activity and catalytic ability. By controlling the conversion process of hydrophobicity and hydrophilicity on the lab-on-paper device combined with diverse origami methods, the dual-mode PEC/colorimetric signal output for the ultrasensitive AFP detection was realized. Under optimal conditions, the proposed dual-mode lab-on-paper device could enable the sensitive PEC/colorimetric diagnosis of AFP in the linear range of 0.09–100 ng mL−1 and 5–100 ng mL−1 with the limit of detection of 0.03 and 1.47 ng mL−1, respectively.
研究领域:
分析化学
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生物传感器
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纳米技术
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2025
·
IF 4.5
Yi Liu,Fuliang Wang
Two highly sensitive amperometric immunosensors for alpha-fetoprotein antigen (AFPAg) detection were fabricated through electrode modification strategies. Typically, during AFPAg detection, electron transfer is mediated by substances such as ferrocene or gold. However, in the case of the prepared zinc oxide electrodes, direct electron transfer was observed between the electrode surface and horseradish peroxidase (HRP). Specifically, by modifying a zinc electrode, pre-heated to 500°C, with AFP antibody (AFPAb) and ferrocene via chitosan, we developed two types of amperometric immunosensors for AFPAg quantification. Under optimized conditions, cyclic voltammetry revealed specific responses of the immunosensors to AFP within two distinct concentration ranges: 1.56 to 12.5 ng/mL and 25.0 to 100.0 ng/mL. The limits of detection were determined to be 0.02 ng/mL and 0.05ng/mL, respectively, at a signal-to-noise ratio of 3. The results demonstrated that electrodes modified with chitosan and AFPAb exhibited high sensitivity, indicating effective immobilization of AFPAb on the electrode surface and facilitating direct electron transfer between the electrode and HRP. Furthermore, when the electrode was additionally modified with ferrocene, a good sensitivity was achieved, suggesting that ferrocene also participated in electron transfer and contributed to signal amplification. We conducted a comprehensive investigation into the factors influencing the performance of the proposed immunosensors. The developed immunosensors exhibited excellent specificity, good reproducibility, repeatability, and long-term stability, making them promising candidates for clinical AFPAg detection.
研究领域:
免疫学
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肿瘤学
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生物传感器
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分子诊断
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转化医学
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临床检验
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纳米医学
✓ 已验证
DOI
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 3
·125-129
·
IF 0.975
Chulei Zhao,Kaihang Cao,Chaoyun Ma,Wenjun Li,Yiju Song,Xiuwen Qiao,Chenglin Hong
In this experiment, a Cu 2 O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml ( S / N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis.
研究领域:
分析化学
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纳米科技
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生物化学
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生物医学工程
·
电化学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Qiang Chu,Yiru Zhang,Wen Chen,Ruoyi Jia,Xin Yu,Yaxuan Wang,Yonglu Li,Yangyang Liu,Xiang Ye,Lushuang Yu,Xiaodong Zheng
Immunosuppression refers to the suppression of the immune response. The immune function of immunocompromised people is not enough to resist bacterial, viral, fungal and other infections, leading to a series of diseases. A large number of experimental data show that polysaccharide compounds are immune modulators, which can enhance the body immunity with little toxic. Meanwhile, it can reduce the side effects of commonly used immunosuppressants, such as cytotoxicity, decreased ability of the body to fight infection, and inhibition of the reproduction of bone marrow hematopoietic cells. It can be used as oral or injectable drugs. In this study, a purified polysaccharide was primarily extracted from the flowers of Apios americana Medik (AAM), which can improve the immunosuppression induced by cyclophosphamide (CTX). The immunoenhancement effect of AFP was evaluated by measuring the body weight, immune organ index, cytokine secretion and antibody generated levels of CTX-induced mice. Our results showed that AFP could significantly improve the above immune indexes, which indicated AFP could alleviate immunosuppression induced by CTX. The study provided a theoretical basis for the promotion, development and application of AAM as a newly introduced food material.
研究领域:
免疫学
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药理学
·
植物学
·
实验医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Oncotarget
·
2017
·
IF 5.168
Hai-Ning Liu,Hao Wu,Yan-Jie Chen,Yu-Jen Tseng,Enkhnaran Bilegsaikhan,Ling Dong,Xi-Zhong Shen,Tao-Tao Liu
Background Many new diagnostic biomarkers have been developed for hepatocellular carcinoma (HCC). We selected two methods with high diagnostic value, the detection of serum microRNAs and metabolomics based on gas chromatography/mass spectrometry (GC/MS), and attempted to establish appropriate models. Methods We reviewed the diagnostic efficiencies of all microRNAs identified by previous diagnostic tests. Then we chose appropriate microRNAs to validate the diagnostic efficiencies, and determined the optimal combination. We included 66 patients with HCC and 82 healthy controls (HCs) and detected the expression of the microRNAs. GC/MS analysis was performed, and we used three multivariate statistical methods to establish diagnostic models. The concentration of alpha feto-protein (AFP) was determined for comparison with the novel models. Results 82 published studies and 92 microRNAs were ultimately included in this systematic review. Seven microRNAs were selected for further validation of their diagnostic efficiencies. Among which, miR-21, miR-106b, miR-125b, miR-182 and miR-224 had a significantly different expression in HCC patients. The combination of miR-21, miR-106b and miR-224 had the highest area under the curve (AUC) at 0.950 with a sensitivity of 80.3% and a specificity of 92.7%. The GC/MS analysis exhibited an excellent diagnostic value and the AUC reached 1.0. In comparison, the AUC of the traditional biomarker, AFP, was 0.755. Conclusion MicroRNAs and metabolomics shows promising potential as new diagnostic methods due to their high diagnostic value compared with traditional biomarkers.
研究领域:
肿瘤学
·
分子生物学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Wenjie Zhu,Hong Qian,Shengya Cao,Wei Xia,Xilong Wang,Jing Jin,Xin Wang,Hao Zhang,Dongsheng Liu,Ying Chen
Background The early detection of Hepatocellular Carcinoma (HCC) is crucial for improving patient survival rates.Early diagnosis of HCC can significantly enhance treatment outcomes and reduce disease progression. Antigen detection of tumor markers is one of the important diagnostic methods for HCC. However, Traditional antigen detection methods often rely on heavy detection equipment, involve lengthy turnaround times, and must be conducted in laboratory settings. Therefore, there is a clear need for a portable, low-skill, rapid sample-to-result detection method for early HCC biomarkers. Results We propose a new platform based on electrowetting-on-dielectric digital microfluidic(EWOD-DMF) for the detection of early-stage HCC biomarkers, enabling the quantitative measurement of Alpha-Fetoprotein (AFP), the proportion of AFP-L3 in total AFP (AFP-L3%), and Des-Gamma-Carboxy Prothrombin (DCP). First, serum samples are processed through the microfluidic system, achieving the separation of AFP-L3 within 10 min. Next, immunoassays are performed within 15 min, using magnetic particles to capture biomarkers such as AFP, AFP-L3, and DCP, followed by enzymatic reactions that generate detectable signals. Each chip can simultaneously detect three biomarkers from five different samples, allowing for a total of fifteen targets to be tested, with only approximately 2.4 μL of serum required for each biomarker detection. Ultimately, data are analyzed with dedicated software to quantitatively measure the HCC biomarkers. The detection limits for AFP or AFP-L3 and for DCP are 0.24 ng/mL and 1.89 ng/mL, respectively. Significance This study presents a EWOD-DMF platform for early-stage HCC diagnosis, capable of simultaneously detecting multiple samples and biomarkers, thus improving detection efficiency and diagnostic accuracy. Moreover, the platform has POCT capability, with advantages in portability and cost-effectiveness, providing clinicians and primary healthcare institutions with a fast and convenient solution for early-stage HCC diagnosis.
研究领域:
医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2026
·
IF 4.5
Xin Yan,Huina Tang,Rao Fu,Xiaobing Chen,Lulu Wang,Shirong Zhang,Xue Zhou
We proposed a surface plasmon resonance (SPR) biosensor based on a multimode-multi-core-multimode fiber (MMF-MCF-MMF) structure, where the cladding of the MCF was etched using hydrofluoric acid (HF). The sensing region of the etched MCF was sequentially coated with Ag-GeSe-Au triple-layer films for detecting alpha-fetoprotein (AFP), a key biomarker of liver cancer cells. The structure and film thickness of the sensing region were optimized to enhance the sensitivity and detection accuracy of the fiber optic sensor. By specifically immobilizing AFP antibodies on the sensor surface, directional detection of AFP was achieved. The sensor demonstrated an AFP detection range of 0-2000 ng/mL with a sensitivity of 1.477 nm/log(ng/mL) and a low limit of detection (LoD) of 1.072 ng/mL. The proposed MCF sensor integrates optical fiber sensing with biological detection. To enhance the detection capability for low-concentration biomolecules, efforts have been focused on improving the sensor's detection accuracy and sensitivity. The biosensor enables highly specific and trace-level detection, holding significant potential for preventive screening and early diagnosis of liver cancer.
✓ 已验证
DOI
高影响因子
Surfaces and Interfaces
·
2021
·
IF 4.837
Xinxin Mo,Yu Wang,Qiyou Xiao,Xitong Zhou,He Li
Alpha fetoprotein(AFP) is an important tumor biomarker associated with some diseases, and its accurate determination is very important for clinical detection. In this paper, a novel conjugated polymer(CP) containing multi-carboxylic group was synthesized and used to fabricate photoelectrochemical biosensor based on CP sensitized hyperbranched titanium dioxide for detection of AFP in serum. The prepared materials were characterized by FT-IR, IR, NMR and UV-Vis, respectively. The absorption wavelength of the hyperbranched titanium dioxide shifted from ultraviolet region(365nm) to visible region(395nm) after modified with CP compound. Which is conducive to broaden the application of titanium dioxide in biomedicine field due to reducing protein denaturation . The results showed that the photoelectrochemical biosensor has a good linear range from 0.1 ng•mL −1 to 1.0×10 2 ng•mL −1 with detection limit of 0.03 ng•mL −1 (S/N=3). The proposed biosensor exhibited the good selectivity , stability and high sensitivity for detecting AFP in serum. This strategy is expected to be used to detect AFP in clinical serum.
研究领域:
生物化学
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生物医学工程
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分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Wenjun Li,Yue Yang,Chaoyun Ma,Yiju Song,Xiuwen Qiao,Chenglin Hong
Traditional sandwich-type electrochemical immunosensors can only detect single tumor markers because signal interference occurs when detecting multiple tumor markers. In this work, an electrical signal difference strategy was proposed for the accurate detection of multiple tumor markers. We labeled PdAgCeO 2 mesoporous nanospheres with a carcinoembryonic antigen (CEA) secondary antibody and MnO 2 nanosheets labeled with an alpha-fetoprotein (AFP) secondary antibody. The two electrical signal tags were mixed and incubated on a prepared immunosensor to catalyze H 2 O 2 and generate an electrical signal I 1 (i-t ampere curve). When 2.5 mM ascorbic acid solution (AA) was added to 20 mL of PBS solution at pH = 6.5 for 180 s, an electrical signal I 2 was generated. I 2 was the current response of the CEA antigen concentration, and the electrical signal difference ΔI = I 1 – I 2 was the current response of the AFP antigen. Thus, the immunosensor accurately detected the AFP and CEA tumor markers. This method was called the electrical signal difference strategy. The proposed single-use immunosensor detected CEA antigens in a range of 0.001 ng/mL-40 ng/mL, and the detection limit was 0.5 pg/mL; the detection range of the AFP antigen was 0.005 ng/mL-100 ng/mL, and the detection limit was 1 pg/mL. Therefore, this study provides new ideas and strategies for accurate clinical detection of multiple tumor markers.
研究领域:
生物医学工程
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Yueshuang Xu,Xiaoping Zhang,Chengxin Luan,Huan Wang,Baoan Chen,Yuanjin Zhao
Barcodes-based suspension array have for demonstrated values in multiplex assay of tumor markers. Photonic barcodes which are encoded by their characteristic reflection peaks are the important supports for suspension array due to their stable code, low fluorescent background and high surface-volume ratio. Attempts to develop this technology tend to improve the function of the photonic barcodes. Here, we present a new type of hybrid hydrogel photonic barcodes for efficient multiplex assays. This photonic barcodes are hybrid inverse opal hydrogel composed of poly(ethylene glycol) diacrylate (PEG-DA) and agarose . The polymerized PEG-DA hydrogel could guarantee the stabilities of the inverse opal structure and its resultant code, while the agarose could offer active chemical groups for the probe immobilization and homogeneous water surrounding for the bioassay. In addition, the interconnected pores inverse opal structure could provide channels for biomolecules diffusing and reaction into the voids of barcodes. These features imparted the hybrid hydrogel photonic barcodes with limits of detection (LOD) of 0.78 ng/mL for carcinoembryonic antigen (CEA) and 0.21 ng/mL for α-fetoprotein (AFP), respectively. It was also demonstrated that the proposed barcodes showed acceptable accuracy and detection reproducibility, and the results were in acceptable agreement with those from common clinic method for the detections of practical clinical samples. Thus, our technique provides a new platform for simultaneous multiplex immunoassay .
研究领域:
生物医学工程
·
分析化学
·
分子诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 20
·6385
·
IF 3.5
Peng Sun,Mingbin Liu,Yongxin Zhang,Chaoran Liu,Xun Yang
Chronic hepatitis B poses a serious threat to human health and life, and early diagnosis is essential to improving patient cure rates. Hepatitis B virus (HBV) and Alpha-fetoprotein (AFP) are two key biomarkers for diagnosing chronic hepatitis B. In this study, we propose a silicon nanowire array field effect transistor (SiNW-array FET) biosensor that enables highly sensitive, real-time, and low-cost joint detection of both HBV and AFP. The SiNW-array FET is fabricated using traditional micro-nano fabrication techniques such as self-limiting oxidation and anisotropic etching, and its morphology and electrical properties were tested. The results show that the diameters of the fabricated silicon nanowires (SiNWs) are uniform and the SiNW-array FET exhibits a strong output signal and high signal-to-noise ratio. Through specific chemical modification on the surface of SiNWs, the SiNW-array FET is highly sensitive and specific to HBV-DNA fragments and AFP, with ultralow detection limits of 0.1 fM (HBV-DNA) and 0.1 fg/mL (AFP). The detection curve of the SiNW-array FET exhibits good linearity within the HBV-DNA concentration range of 0.1 fM to 100 pM and AFP concentration range of 0.1 fg/mL to 1000 pg/mL. More importantly, the device could also detect HBV-DNA successfully in serum samples, laying a solid foundation for the highly sensitive clinical detection of chronic hepatitis B.
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Xiaohong Tan,Bo Wang,Xuyang Chen,Yuge Liu,Dang Wu,Jintao Liang,Guiyin Li
Background Golgi protein 73 (GP73) has emerged as a promising serological biomarker for hepatocellular carcinoma (HCC), with its combination with alpha-fetoprotein (AFP) substantially improving diagnostic precision in early-stage HCC detection. To address this clinical need, we constructed an electrochemical dual-aptasensor for parallel quantification of GP73&AFP. Results GP73 aptamer (GP73 Apt ) functionalized with reduced graphene oxide-ferrocene-polyaniline (rGO-Fc-PANi) nanocomposites and AFP aptamer (AFP Apt ) conjugated with nitrogen-doped reduced graphene oxide-cuprous oxide (NrGO-Cu 2 O) nanostructures were employed as the dual-signal probes. Upon GP73&AFP binding, the GP73 Apt -GP73 interaction induced double-helix dissociation of aptamer-complementary DNA (cDNA) complexes, stabilizing the Fc-mediated electrochemical structure and amplifying Fc redox current. Conversely, AFP Apt -AFP interaction induced steric hindrance through aptamer-protein complex formation, synergistically combined with dielectric property changes to suppress Cu 2 O redox signals. Under optimal conditions, the aptasensor demonstrated logarithmic linear ranges of 0.001–100.0 ng/mL for both biomarkers, achieving lower LOD of 0.48 pg/mL (GP73) and 1.77 pg/mL (AFP). Additionally, GP73&AFP were directly measured in human serum samples and demonstrated excellent reproducibility with RSD of 1.15–3.52 % for GP73 and 3.96–5.97 % for AFP. Significance This work presents a groundbreaking advancement in HCC diagnostics through the development of a dual-mode electrochemical aptasensor. The system's sensitive simultaneous detection capability, combined with operational stability in complex biological matrices, positions it as a transformative tool for early-stage HCC screening and management.
研究领域:
生物传感技术
·
肿瘤诊断
·
纳米材料
·
电化学分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）