BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
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2017
·Vol. 65
·期 3
·323-327
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IF 1.413
GuoQing Ying,MinJun Wang,Yu Yi,JianShu Chen,JianFeng Mei,YanLu Zhang,ShuQing Chen
An electrochemical biosensor that used an aptamer as a biological element was constructed to detect endotoxin. Biolayer interferometry was used to obtain the affinity constant of an aptamer for lipopolysaccharide, which had an equilibrium dissociation constant of 22.9 nM. The amine-terminated aptamer was then assembled on a gold electrode surface using 3-mercaptopropionic acid as an intermediate linker. The modification of the gold electrode was confirmed by cyclic voltammetry and electrochemical impedance spectroscopy. In the range of 0.001–1 EU/mL, the increase in electron transfer resistance of the biosensor was linear with the logarithmic value of the endotoxin concentration. The constructed biosensor exhibits sensitivity and a low limit of detection.
研究领域:
生物化学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
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2022
·
IF 5.76
Hao Fan,Ying Wu,Tongfu Huang,Nian Hong,Hanfeng Cui,Guobing Wei,Fusheng Liao,Jing Zhang
As an artificial nanomachine, a DNA walker demonstrates the potential for biosensing. In this study, a highly integrated, biostable, and autonomous electrochemical DNA walker sensor was rationally designed by a simple assembly of a Mn 2+ -dependent DNAzyme-powered DNA walker with nanoscale Mn 2+ @MOFs containing free carboxylic acid groups UiO-66(Zr)-(COOH) 2 . In this study, the release of Mn 2+ from Mn 2+ @MOFs was exploited to drive the autonomous and progressive operation of the DNA walker, and the DNAzyme-driven DNA walker was constructed by the co-modification of walking strands and track strands onto the gold electrode (GE) surface. The walking strand was a single-stranded DNA containing a DNAzyme sequence, which was pre-silenced by the locking strand. The track strand was a specially designed DNA sequence that the target can hybridize with the locking strand; hence, the walking strand is unlocked, and the liberated DNAzyme catalyzes the cleavage of track strands to drive the DNA walker operation, shifting tetraferrocene away from the electrode and producing a significant signal change. A detection limit of 38 fM was obtained with our new system, exhibiting a wide linear range from 1.5625 × 10 −9 M to 1 × 10 −13 M. The proposed approach provided a novel means for constructing an highly integrated, automated, and DNAzyme-driven DNA walker for bioanalysis.
研究领域:
生物传感
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry A
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2022
·Vol. 10
·期 9
·4858-4869
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IF 14.511
Yancong Qiao,Jinming Jian,Hao Tang,Shourui Ji,Ying Liu,Haidong Liu,Yuanfang Li,Xiaoshi Li,Fei Han,Zhiyuan Liu,Tianrui Cui,Guangyang Gou,Lelun Jiang,Yi Yang,Bingpu Zhou,Tian-Ling Ren,Jianhua Zhou
A pressure sensor is an important device in daily life, especially for physiological signal monitoring. However, to realize an intelligent pressure sensor, more features should be achieved, such as high sensitivity, large linearity range, in situ signal processing, and automatic analysis. In this article, inspired by the reinforced concrete structure, a nanomesh-reinforced graphene pressure sensor (NRGPS) has been designed and realized with not only excellent mechanical performance but also water vapor permeability. Unlike the negative-resistance pressure sensor, the resistance of the NRGPS increases under a larger pressure, which greatly increases the measuring range. Thanks to the nanomesh skeleton, the NRGPS has ultra large linearity (1 MPa), high sensitivity (4.19 kPa−1), and excellent stability (more than 10 000 cycles). To explain the sensing mechanism of the NRGPS, a finite element model was proposed for the microstructure of nanomesh-reinforced graphene. With the aid of large linearity and high sensitivity, the NRGPS can simulate the mechanical Metal–Oxide–Semiconductor Field Effect Transistors (MOSFET) and realize the in situ pulse signal amplification. Finally, an intelligent tactile sensor was achieved by combining the NRGPS with a convolutional neural network. Convex Braille numbers can be distinguished by the intelligent tactile sensor with an accuracy of 88%. This work has potential in the intelligent diagnosis and tactile reconstruction field.
研究领域:
材料科学
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生物医学工程
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电子工程
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2017
·
IF 4.95
Nuo Duan,Wenhui Gong,Shijia Wu,Zhouping Wang
An improved SELEX technique was developed for selecting aptamers against ractopamine (RAC) by immobilizing ssDNA library on the magnetic beads. After sixteen selection rounds, a highly enriched ssDNA pool was sequenced and nine families were grouped according to their homology and secondary structures analysis. One representative aptamer candidate from each family was picked out for binding affinity identification by graphene oxide (GO) adsorption platform. The aptamer RAC-6 was demonstrated as the optimal aptamer with high specificity and dissociation constant (K d ) value of 54.22 ± 8.02 nM. To prove the potential application of aptamer RAC-6 in the quantitative determination of RAC, a fluorescent bioassay with aptamer RAC-6 was developed. The linear range for RAC was from 0.10 ng/mL to 100 ng/mL and the limit of detection was as low as 0.04 ng/mL. Furthermore, the method was validated for the analysis of RAC spiked real samples, and the recoveries were between 82.57% and 104.65%.
研究领域:
分子生物学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
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2020
·Vol. 8
·期 10
·5275-5283
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IF 11.301
Yufan Hao,Quan Li,Benqiao He,Bo Liao,Xianhui Li,Mengyang Hu,Yanhong Ji,Zhenyu Cui,Mohammad Younas,Jianxin Li
Nanofiltration (NF) membranes with high permeability and selectivity are urgently needed to reduce energy consumption and improve separation efficiency in desalination and wastewater treatment. Herein, an ultrahighly permeable-selective NF membrane mediated by an in situ formed interlayer is designed and prepared through chitosan (CS) assisted interfacial polymerization of piperazine (PIP) in water with trimesoyl chloride (TMC) in n-hexane. CS exists in the form of free molecules and nanoparticles (CSPs) and in situ forms an interlayer due to its slower diffusion rate compared with PIP during interfacial polymerization and mediates the structure and performance of the NF membrane. Through controlling the CS concentration and aging time of CSPs in an aqueous amine solution, the NF membrane surface morphology can be systematically tuned from nodular to vermicular and to a convex structure, while the thickness of the polyamide layer obtained can be maintained at about 20 nm. The resulting NF membrane shows an unprecedented separation performance with a pure water permeance up to 45.2 L m−2 h−1 bar−1, equivalent to about 5-fold that of the control PIP/TMC NF membrane, while maintaining a Na2SO4 rejection of 99.3%. The superior performance is attributed to a synergistic effect of the reduced thickness of the polyamide layer, increased special surface area with negative charges, and low-resistance water nanochannels in the interlayer. This work provides a cost-effective and simple route to fabricate ultrahighly permeable-selective NF membranes.
研究领域:
材料科学
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环境工程
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化学工程
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DOI
高影响因子
Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 7
·1991-1998
·
IF 6.641
Jiaxin Zhang,Xiang Zhang,Haiwei Feng,Ziwei Yu,Jiaming Zhang,Shihao Liu,Letian Zhang,Wenfa Xie
At present, hybrid organic light-emitting devices (HyOLEDs) employing inorganic metal oxides as carrier injection/transport materials are widely studied because they are able to improve the poor stability of conventional devices. However, the stability can only be slightly improved by using a metal oxide as an electron transport layer as the base of the device. On the basis of the above-mentioned hybrid devices, we have successfully prepared a stable device by introducing an ultra-thick inorganic metal oxide MoO3 layer (105 nm) as a hole transport layer on top of the light-emitting layer to prevent erosion caused by moisture and oxygen in air. The luminance of the unpackaged device after storing in the atmosphere for 7 h is 8 times higher than that of conventional devices. In addition, due to the efficient hole injection ability and excellent carrier mobility of MoO3, even if the thickness of the device is significantly increased, the turn-on voltage is still lower than that of conventional devices. Moreover, electrons and holes are balanced in the device, so the efficiencies are significantly higher than those of conventional devices and HyOLEDs using inorganic metal oxides as electron transport layers.
研究领域:
材料科学
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光电技术
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有机电子学
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产品关联
DOI
高影响因子
AESTHETIC PLASTIC SURGERY
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2026
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IF 2.7
Li Tian-Xi,Lang Ming-Fei,Su Di-Ya,Zhang Chen,Wang Shi-Hui,Dong Xiao-Ying,Han Cheng,Wang Jie-Qing,Sun Jing
Objective Despite its good biocompatibility and processability, the severe surface hydrophobicity of polydimethylsiloxane (PDMS) limits its biomedical applications. This study aimed to enhance the surface properties of PDMS by depositing gelatin onto it via polydopamine (PDA) mediation and to evaluate the resulting cell–material interactions. Methods and Results The 1% gelatin-modified PDA-PDMS (G-PDA-PDMS) group showed the highest cell viability at 48 h. Surface hydrophilicity was significantly improved, with the contact angle reduced to 21.97° ± 2.06 ( p <0.01). XPS confirmed successful modification, showing increased nitrogen signal and characteristic C=O/C-N peaks on G-PDA-PDMS. AFM revealed significantly higher surface roughness and altered nano-topography compared to PDMS ( p <0.05). After interacting with cells, it was observed that the cell viability of G-PDA-PDMS was significantly higher than that of the other two groups ( p <0.05). FITC/DAPI fluorescence staining showed that the number of cells in G-PDA-PDMS was higher than that in PDMS ( p <0.05), and it exhibited a better cell spreading area ( p <0.05). Calcein AM/PI fluorescence staining showed that the proportion of red fluorescence in PDA-PDMS and G-PDA-PDMS decreased significantly, while the green fluorescence increased significantly, indicating that they had a better cell survival rate compared with PDMS ( p <0.05). The cell scratch test showed that the cell migration rate of G-PDA-PDMS was higher than that of PDA-PDMS ( p <0.05). At the molecular level, G-PDA-PDMS significantly upregulated the mRNA expression of TGF-β1 , COL3A1 , COL1A1 , and α-SMA ( p <0.05), which was corroborated by increased TGF-β1 protein production ( p <0.05). Conclusion Gelatin was successfully modified onto PDA-PDMS. The resulting G-PDA-PDMS material significantly improves surface hydrophilicity, enhances fibroblast proliferation and adhesion, and upregulates key extracellular matrix-related gene and protein expression, demonstrating excellent bioactivity and potential for biomedical applications. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable
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高影响因子
Cell Chemical Biology
·
2026
·Vol. 33
·期 2
·227-240
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IF 9
Jinyao Chen,Ming Zhang,Yeji Wang,Zhiyu Xie,Fei Yang,Hongjun Yu,Di Zhang,Min Fang,Xiaoyi Luan,Xueyang Jin,Xue Wang,Yanan Qiao,Shicun Zheng,Xiuyun Li,Wenqiang Chang,Hongxiang Lou
The overexpression of drug exporters is a well-known mechanism driving antifungal drug resistance. Here, we investigate that converting the exporter to function as a drug importer provides an alternative approach to combat drug resistance. Using high-throughput screening, we identified quaternary ammonium compounds exhibiting hypersensitivity in Candida albicans strains overexpressing MDR1 . Further experiments involving CRISPR-Cas9-mediated deletion in C. albicans , heterologous expression of Ca Mdr1 in Saccharomyces cerevisiae and construction of Ca Mdr1-containing proteoliposomes confirmed Mdr1 acts as an importer of MKT-077, a potent cyanine derivative highly selective against MDR1 -overexpressing strains. By combining computational simulations and the site-directed mutagenesis, critical residues within the transmembrane domains of Ca Mdr1 involved in MKT-077 import were further identified. Moreover, MKT-077 exhibited therapeutic efficacy in Galleria mellonella infected with Mdr1-overexpressing C. albicans . This work opens an avenue to convert drug resistance into drug sensitivity-a paradigm-shifting strategy in antifungal drug discovery.
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DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 49
·36622-36632
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IF 3.9
Lijun Xu,Qian Tong,Bing Hu
Through a simple room-temperature process, different amounts of Keggin-type quaternary ammonium silicotungstate were successfully encapsulated into the metal–organic framework (MOF) material ZIF-67. The catalysts were characterized using Fourier transform infrared (FT-IR) spectroscopy, X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and BET analysis. An extraction and catalytic oxidation desulfurization system was studied using H2O2 as an oxidant and a deep eutectic solvent (DES) as an extractant. Using the 43.06%-SiW12@ZIF-67 composite under optimal reaction conditions, DBT present in a model oil could be deeply and effectively removed. The catalyst was reused 6 times, and the desulfurization rate still exceeded 90%. Finally, a possible desulfurization mechanism is proposed.
研究领域:
催化
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环境化学
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材料科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 8.2
Minning Wang,Sen Zhang,Yuxin Kong,Bingjun Wang,Shijie Wang,Jing Guo,Yuxiang Li,Wei Ma
Machine vision systems possess the abilities of converting captured image signals into digital signals and further processing these signals to extract features, replacing human eyes for measurement and judgment. Optoelectronic memristors with the functionalities of photodetection, memory, and processing are a promising candidate used in a machine vision system. However, the existing optoelectronic memristors are limited by inevitable electrical signal input, complex fabrication processes, inhomogeneous integration of different materials, and high cost. In this work, we designed and fabricated a single organic mixed ionic-electronic conductor (OMIEC)-based all-optical-controlled synaptic device (AOCSD), which can achieve three functionalities of reading, light-writing, and light-erasing using three optical-controlled modules, separately. During device operation, there is no electrical input, which reduces the complexity of the programming. Furthermore, this OMIEC-based AOCSD can be used to emulate various visual synaptic plasticity behaviors in an all-optical-controlled manner, making it possible to realize passive machine vision hardware near the edge.
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DOI
PubMed
高影响因子
IEEE Access
·
2021
·
IF 3.367
Fujun Ji
In terms of the leap-forward development for education experiment, both the Chinese and English educational experiment have been very successful, while the progress of the children’s mathematics education experiment in China has been relatively slow. It is believed that the main reason for this is the lack of an efficient cognitive tool or system that supports students’ study and exploration and correspondingly covers the whole curriculum of children’s math education. In recent years, basic mathematics education cognitive tools have undergone rapid development, whereas the lack of a common normalization method for continued in-depth inquiry of the relationships among Objects, Algebra, and Geometry (OAG) has led to the fragmentation of students’ mathematical knowledge. Thus, it is difficult for students to develop a systematic understanding of mathematics. Therefore this project attempts to construct a Children’s Mathematics Exploring System (CMES), which integrates with cognition theory, method, and contents of basic mathematics education to query the essence of the relationships among OAG. Specifically, the functions of CMES include the following: (1) providing cognitive tools with typical characteristics for the relationships among OAG; (2) offering a general normalized open system on which sustainable in-depth research can be conducted and a conversion tool through which the complete textbook unit of the basic mathematics education can be converted to reveal the relationships among OAG; and (3) presenting a set of cognitive theories, methods, and a system of basic mathematics education to further explore the relationships among OAG. This project will accelerate the development of cognitive technology in basic mathematics education, widen its practical applications, reduce the difficulty of mathematics cognition, and finally improve the quality and efficiency of basic mathematics education.
研究领域:
数学教育
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认知科学
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教育技术
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课程开发
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DOI
高影响因子
Microsystems & Nanoengineering
·
2024
·Vol. 10
·期 1
·1-17
·
IF 7.3
Yang Chengxiu,Hu Jiafei,Liu Lihui,Wu Shaowei,Pan Mengchun,Liu Yan,Wang Haomiao,Li Peisen,Zhang Qi,Qiu Weicheng,Luo Huihui
The perception of fish locomotion is important for understanding their adaptive behaviors and ethological characteristics. However, the main strategy used for extracting fish attitudes involves the use of a vision-based monitoring system, which is limited in its range of observation and cannot perform tracking for long times. Here, we report the use of a wearable tagging electronic device, referred to as an underwater vest, to capture the surrounding flow field disturbances triggered by swimming or momentary postural changes. All of these goals were achieved by integrating a pair of pseudocapacitive pressure-sensing units and a flexible circuit board. Notably, additional conditions, such as variable hydraulic pressures and minimal changes in fish posture, require high stability and sensitivity of the sensing units. Thus, hybrid hydrogel electrodes were developed through cross-linking MXene with holey-reduced graphene oxide nanosheets and further modification with 1-ethyl-3-methylimidazolium dicyanamide ionic liquids, which increased the interfacial capacitance and long-term interfacial activity of the MXene. Consequently, the sensing unit exhibited ultrahigh sensitivity ( S max ~136,207 kPa −1 ) in an aquatic environment for 60 days and superior high-pressure resolution (10 Pa) within a wide working range of 1 MPa. Ultimately, an underwater vest integrated with such sensing units clearly distinguished and recorded fish locomotion. We believe that the designed device may open avenues in flow field monitoring and ocean current detection and provide new insights into the development of sensitive underwater tagging.
研究领域:
生物工程
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材料科学
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海洋学
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DOI
高影响因子
危险品化学品经营许可证（带存储）