ACTA PHARMACOLOGICA SINICA
·
2017
·Vol. 38
·期 7
·998-1008
·
IF 3.223
Zhou Bing,Zhou Dan-li,Wei Xiao-hong,Zhong Rong-yu,Xu Jie,Sun Liao
Although the pathogenesis of non-alcoholic fatty liver disease (NAFLD) is not completely understood, the increased influx of free fatty acids (FFAs) into the liver and the FFA-induced hepatic endoplasmic reticulum (ER) stress are two crucial pathogenic processes in the initiation and development of NAFLD. In this study we investigated the effects of astragaloside IV (AS-IV), a bioactive compound purified from Astragali Radix , on FFA-induced lipid accumulation in hepatocytes and elucidated the underlying mechanisms. Human HepG2 cells and primary murine hepatocytes were exposed to FFAs (1 mmol/L, oleate/palmitate, 2:1 ratio) with or without AS-IV for 24 h. Exposure to FFAs induced marked lipid accumulation in hepatocytes, whereas co-treatment with AS-IV (100 μg/mL) significantly attenuated this phenomenon. Notably, AS-IV (50–200 μg/mL) concentration-dependently enhanced the phosphorylation of AMPK, acetyl-CoA carboxylase (ACC) and SREBP-1c, inhibited the accumulation and nuclear translocation of mature SREBP-1 and subsequently decreased the mRNA levels of lipogenic genes including acc1 , fas and scd1 . AS-IV treatment also concentration-dependently attenuated FFA-induced hepatic ER stress evidenced by the reduction of the key markers, GRP78, CHOP and p-PERK. Pretreated the cells with the AMPK inhibitor compound C (20 μmol/L) greatly diminished these beneficial effects of AS-IV. Our results demonstrate that AS-IV attenuates FFA-induced ER stress and lipid accumulation in an AMPK-dependent manner in hepatocytes, which supports its use as promising therapeutics for hepatic steatosis.
研究领域:
药理学
·
肝病学
·
细胞生物学
·
代谢疾病
✓ 已验证
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2016
·Vol. 37
·期 7
·941-949
·
IF 3.166
Sun Hui-ping,Su Jing-han,Meng Qing-shuo,Yin Qi,Zhang Zhi-wen,Yu Hai-jun,Zhang Peng-cheng,Wang Si-ling,Li Ya-ping
Aim: To improve the therapeutic efficacy of cancer treatments, combinational therapies based on nanosized drug delivery system (NDDS) has been developed recently. In this study we designed a new NDDS loaded with an anti-metastatic drug silibinin and a photothermal agent indocyanine green (ICG), and investigated its effects on the growth and metastasis of breast cancer cells in vitro . Methods: Silibinin and ICG were self-assembled into PCL lipid nanoparticles (SIPNs). Their physical characteristics including the particle size, zeta potential, morphology and in vitro drug release were examined. 4T1 mammalian breast cancer cells were used to evaluate their cellular internalization, cytotoxicity, and their influences on wound healing, in vitro cell migration and invasion. Results: SIPNs showed a well-defined spherical shape with averaged size of 126.3±0.4 nm and zeta potential of −10.3±0.2 mV. NIR laser irradiation substantially increased the in vitro release of silibinin from the SIPNs (58.3% at the first 8 h, and 97.8% for the total release). Furthermore, NIR laser irradiation markedly increased the uptake of SIPNs into 4T1 cells. Under the NIR laser irradiation, both SIPNs and IPNs (PCL lipid nanoparticles loaded with ICG alone) caused dose-dependent ablation of 4T1 cells. The wound healing, migration and invasion experiments showed that SIPNs exposed to NIR laser irradiation exhibited dramatic in vitro anti-metastasis effects. Conclusion: SIPNs show temperature-sensitive drug release following NIR laser irradiation, which can inhibit the growth and metastasis of breast cancer cells in vitro .
研究领域:
肿瘤学
·
药剂学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2016
·Vol. 37
·期 8
·1091-1100
·
IF 3.166
Xiong Yong-jian,Chu Hong-wei,Lin Yuan,Han Fang,Li Ya-chan,Wang Ai-guo,Wang Fu-jin,Chen Da-peng,Wang Jing-yu
Aim: Postoperative ileus (POI) is a postoperative dysmotility disorder of gastrointestinal tract, which remains one of the most perplexing problems in medicine. In the present study we investigated the effects of hesperidin, a major flavonoid in sweet oranges and lemons, on POI in rats. Methods: SD rats were administered hesperidin (5, 20, and 80 mg·kg −1 ·d −1 , ig) for 3 consecutive days. POI operation (gently manipulating the cecum for 1 min) was performed on d 2. The gastrointestinal motility and isolated intestinal contraction were examined 1 d after the operation. Then the myosin phosphorylation and inflammatory responses in cecum tissue were assessed. Smooth muscle cells were isolated from rat small intestine for in vitro experiments. Results: The gastric emptying and intestinal transit were significantly decreased in POI rats, which were reversed by administration of hesperidin. In ileum and cecum preparations of POI rats in vitro , hesperidin (2.5–160 μmol/L) dose-dependently increased the spontaneous contraction amplitudes without affecting the contractile frequency, which was blocked by the myosin light chain kinase (MLCK) inhibitor ML-7 or verapamil, but not by TTX. Furthermore, administration of hesperidin increased the phosphorylation of MLC 20 in the cecum tissue of POI rats. Moreover, administration of hesperidin reversed the increased levels of inflammatory cytokines, iNOS and COX-2 in cecum tissue of POI rats. In freshly isolated intestinal smooth muscle cells, hesperidin (5–80 μmol/L) dose-dependently increased the intracellular Ca 2+ concentration as well as the phosphorylation of MLC 20 , which was abrogated by ML-7 or siRNA that knocked down MLCK. Conclusion: Oral administration of hesperidin effectively alleviates rat POI through inhibition of inflammatory responses and stimulation of Ca 2+ -dependent MLC phosphorylation.
研究领域:
药理学
·
胃肠病学
·
外科
✓ 已验证
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2016
·Vol. 37
·期 11
·1467-1480
·
IF 3.166
Fu Yu,Lin Qing,Gong Tao,Sun Xun,Zhang Zhi-rong
Aim: We previously reported a novel triptolide (TP)-glucosamine conjugate (TPG) that specifically accumulated in kidneys and protected renal function from acute ischemia/reperfusion (I/R) injury in rats. In this study we further examined the molecular mechanisms underlying the renoprotective action of TPG. Methods: The renal-targeting of TPG was investigated in a human proximal renal tubular epithelial cell line (HK-2) by measuring cell uptake of TP or TPG. The effects of TP or TPG on cell cycle distribution and apoptosis rate of HK-2 cells were assessed, and the activities of caspase-3 and caspase-9 were also measured. SD rats were subjected to bilateral renal ischemia by temporarily clamping both renal pedicles. The rats were administered TP (4.17 μmol·kg −1 ·d −1 , iv) or TPG (4.17 μmol·kg −1 ·d −1 , iv) for 3 d before the renal surgery. The kidneys were harvested after 24 h of recovery from the surgery. The levels of oxidative stress, proinflammatory cytokines, chemotactic cytokines and intracellular adhesion molecules in kidneys were examined. Results: The uptake of TPG in HK-2 cells was 2–3 times higher than that of TP at the concentrations tested. Furthermore, TPG targeting the proximal tubules was mediated through interactions with megalin receptors. TP (40–160 nmol/L) concentration-dependently increased G 2 /M arrest, apoptosis and caspase-3/caspase-9 activity in HK-2 cells, whereas the same concentrations of TPG did not show those features when compared with the control group. In I/R-treated rats, TPG administration caused more robust down-regulation of proinflammatory cytokines (TNF-α, IL-6, IL-1, TGF-β) and chemotactic cytokines (MCP-1) in the kidneys compared with TP administration, suggesting the inhibition of the proliferation and accumulation of lymphocytes. And TPG administration also caused more prominent inhibition on the levels of oxidative stress and intracellular adhesion molecules in the kidneys, compared with TP administration. Conclusion: The renal-targeting TPG is more effective and less toxic than TP, in amelioration of I/R-induced rat renal injury, which may provide a new avenue for the treatment of acute kidney injury.
研究领域:
药理学
·
肾病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2026
·
IF 4.6
Wu Xiao,Yuan Jinyu,Li Jingpan,Liu Yue,Li Biao,Yang Jianxiao
High-yield and ultra-pure rayon-based carbon fiber (RCF) mats were successfully fabricated via a synergistic catalytic pyrolysis strategy using ammonium persulfate (APS) and caprolactam (CPL), aiming to overcome the conventional trade-off between carbon yield and catalyst-derived impurities. The catalytic mechanism, pyrolysis behavior, and structural evolution and performance were systematically investigated. APS selectively oxidized C6 hydroxy groups of cellulose into carboxyl functionalities and generated an in situ acidic environment, which suppressed levoglucosan formation and reduced the apparent activation energy of pyrolysis. Under these conditions, CPL underwent acid-induced ring opening to form aminohexanoic acid intermediates that reacted with cellulose-derived species through Schiff-base condensation, leading to the formation of covalently cross-linked structures. This cooperative mechanism effectively inhibited depolymerization and enhanced carbon yield during thermal conversion. As a result, the R-C/A-2000 sample achieved a high carbon yield of 31.78%, and developed turbostratic graphite microcrystals with a d 002 spacing of 0.362 nm after graphitization at 2000 °C. Subsequent halogen purification reduced the total impurity concentration to 119.23 ppm, meeting the purity requirements for semiconductor thermal-field insulation materials. The results demonstrated that synergistic regulation of cellulose pyrolysis pathways via CPL/APS catalysis is an effective strategy to simultaneously improve carbon yield and product purity in RCF mat production. Graphical abstract
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2026
·
IF 6
Chao Ma,Li Feng,Junpeng Cui,Zhipeng Hu,Qianqian Chu,Shipeng Xu,Weiqian Chen,Bo Chen,Putsiata Artur,Luoyi Zhang
To improve the service reliability of components used during spent nuclear fuel electrorefining, La 2 Zr 2 O 7 (LZO) protective coatings were prepared on SS 316L by atmospheric plasma spraying (APS) followed by vacuum annealing. The effects of annealing temperature and holding time on the phase structure, apparent porosity, and surface wettability of the LZO coatings were systematically investigated. The optimized coating was then exposed to KCl-LiCl molten salt at 600 °C for up to 640 h to evaluate its corrosion behavior and degradation mechanism. Annealing at 1200 °C for 2 h reduced apparent porosity from 18.96% to 8.21% and suppressed spreading of the salt-containing test solution. Within the initial 80 h, the as-sprayed and annealed coatings gained 1.3 and 0.6 mg·cm -2 , respectively, with apparent mass-change rates of 0.015 and 0.0075 mg·cm -2 ·h -1 . Both coatings shifted from mass gain to mass loss during 80-160 h, and the apparent mass-change rate of LZO-C640 reached -0.0525 mg·cm -2 ·h -1 . During 160-320 h, the apparent rates became positive as connected defects enabled deeper salt penetration and local interaction with the NiCrAlY bond coating. Preferential degradation of Al- and Cr-rich regions exposed and redistributed Ni-bearing material. NiO and NiCr 2 O 4 were subsequently detected at 320 h as secondary products. LaOCl was also detected at 320 h, whereas LiAlO 2 appeared after extended exposure. Throughout the final stage of 320-640 h, LZO-C640 maintained a low positive rate of 0.0075 mg·cm -2 ·h -1 . In contrast, the cumulative mass-change of H/LZO-C640 increased from -0.6 to -0.3 mg·cm -2 as its apparent rate approached zero. After 640 h, the annealed coating retained its overall skeleton, whereas the as-sprayed coating underwent top-layer disintegration. These results indicate that vacuum annealing did not completely prevent degradation, but limited molten-salt ingress and improved the long-term corrosion resistance of LZO coating.
✓ 已验证
DOI
高影响因子
MATERIALS CHARACTERIZATION
·
2025
·
IF 5.5
Xianshu Dong,Tianshun Dong,Jingkun Li,Yanfei Jiang,Binguo Fu,Guolu Li
To improve the wear resistance of Al 30Si coatings, nano-Y₂O₃-reinforced Al 30Si (Y₂O₃/Al-30Si) coatings were fabricated on 6063 aluminum alloy substrates by atmospheric plasma spraying (APS). The effect of nano-Y 2 O 3 addition on the coating was studied in detail. The results show that nano-Y₂O₃ reduced the porosity of the Al 30Si coatings from 8.2 % to 3.1 %. Nano-Y₂O₃ can provide heterogeneous nucleation sites for primary silicon (β-Si) phases, enabling the Y₂O₃/Al-30Si coating to achieve a 57.4 % reduction in β-Si phase size. Correspondingly, the microhardness, elastic modulus and fracture toughness of the coating increased by 11.0 %, 11.2 % and 48.9 %, respectively. Under the present test conditions, the Y₂O₃/Al-30Si coating exhibited a lower coefficient of friction and a 27.2 % reduction in wear volume. Both the Y₂O₃/Al-30Si and Al 30Si coatings exhibited abrasive, oxidative, and fatigue wear mechanisms.
研究领域:
材料科学
·
表面工程
·
金属涂层
·
摩擦学
·
复合材料
✓ 已验证
DOI
高影响因子
JOURNAL OF THERMAL SPRAY TECHNOLOGY
·
2023
·Vol. 32
·期 8
·2460-2477
·
IF 3.1
Wu Duoli,Wu Haotian,Yuan Ziyi,Wei Xinlong,Zhang Chao
To investigate the influence of Mo addition and water vapor content on the high-temperature corrosion resistance of Ni-Al-based coatings, 0Mo, 5Mo, and 10Mo coatings were deposited by APS thermal spray onto stainless steels. The chlorine-induced corrosion test was conducted in an experimental environment simulating a biomass boiler. The results show that adding the Mo element lowers the coating's ability to resist corrosion without water vapor. The coating is more prone to peeling because Mo prevents the coating from eventually forming a protective oxide layer. Water vapor significantly speeds up the production of oxides and modifies their morphology and structure. The combined effects of Mo and water vapor increase the coating's ability to resist corrosion. The development of thick and protective Ni and Al oxide layers on the coated surface is aided by the acceleration of oxide production by water vapor and the alteration of oxide structure by Mo components.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yuying Wang,Linshan Wang,Shiwen Wang,Jingyu Chen,Ruiwen Hu,Zhongrong Chen,Gang Zhao,Yue Cheng
The surface microstructures of Natural Killer (NK) cells, such as microvilli, are essential for target cell recognition and the subsequent formation of immunological synapses. However, traditional cryopreservation protocols (e.g., 10% DMSO and serum) severely damage the cell surface structure, which further impairs cytotoxicity. Although a variety of novel cryoprotectants or ice inhibitors have emerged to improve the post-thaw viability of cells, there are currently few reports on strategies for the preservation of surface microstructures essential for immune synapse formation in natural killer (NK) cells. Here, we introduce a dual-protection strategy that combines the membrane-stabilizing capacity of Astragalus Polysaccharide (APS), a bioactive compound from traditional Chinese medicine, with a hydrogel encapsulation system to mitigate cryo-injury from ice crystal formation. The results demonstrated that, in the absence of DMSO, the experimental group achieved an 85% viability rate for NK-92 cells. Furthermore, the surface microstructure was significantly better preserved compared to conventional protocols, and the corresponding cytokine secretion and cytotoxicity showed no significant difference from the fresh group. This study demonstrates that the synergistic cryoprotective effect of APS and hydrogel, thereby laying a solid foundation for immune cell preservation and cell-based therapies.
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Junjie Tian,Hongmei Liu,Rong Guo,Honghao Sun,Yufeng Chen,Yi Wang,Qian Xu,Yao Zhu,Qing Yao
White light diodes (WLEDs) composed of blue chip and phosphor-converter are widely used in liquid crystal display (LCD) projectors as backlights. And it often needs a condenser lens to irradiate white light onto the LCD screen. In this paper, a large size lenticular shaped phosphors-in-glass (PiG) was prepared by Isobam-APS gel casting for the first time. The PiG could act for converting and condensing in LCD projector. Then a rectangular light spot similar to the shape of LCD screen was obtained to reduce the loss of light. The maximum temperatures of the chip and PiG were 62.1 °C and 57.3 °C respectively after working for 600 s and gradually stabilized by simulation. It was proved that simulation result was reliable through comparing with the actual measured value and the lenticular shaped PiG had good heat dissipation capacity for its large area. In conclusion, this is a successful application of PiG in LCD projector, which has guidance and promoting effect.
研究领域:
光学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Macromolecular Science Part A-Pure and Applied Chemistry
·
2024
·
IF 2.1
Huijie Yan,Yuhang Jiao,Bingrui Jin,Huixuan Zhang,Zhongyu Fu,Shiling Jia,Yunjiao Deng
Aqueous precipitation polymerization is commonly used in the industry due to its efficient heat dissipation, reaction stability and production flexibility. However, its mechanisms and kinetics are still partially unclear. The ammonium persulfate-ammonium sulfite monohydrate (APS-AS) initiated aqueous phase precipitation polymerization of acrylonitrile (AN) at low concentration was deeply investigated, with the APS initiation system as a control. The apparent kinetic equations for the APS and APS-AS initiation systems were determined to be Rp= K[AN]1.706[(NH4)2S2O8]0.704 and Rp= K[AN]3[(NH4)2S2O8]0.704[(NH4)2S2O3]−1.055, respectively. Monitoring AN distribution in the aqueous and polyacrylonitrile (PAN) phases revealed a rapid transition of the reaction site from the aqueous phase to the PAN phase following polymer precipitation. Zeta potential and primary particle number exposed that the nucleation site disparities between initiation systems led to different predominant factors affecting the polymerization rate. The change in molecular weight during polymerization had a maximum due to its dependence on the monomer concentration. The crystallinity of the product initially increased during polymerization, then decreased and eventually stabilized. Scanning electron microscopy (SEM) revealed that the diameter of the internal particles was larger, while the surface particles were smaller after coagulation. These findings offer crucial insights for optimizing aqueous precipitation polymerization and PAN particle property control.
研究领域:
高分子化学
·
化学反应工程
·
材料科学
·
工业聚合过程
✓ 已验证
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2023
·
IF 5.4
Duoli Wu,Yi Li,Ziyi Yuan,Liao Xiang,Xinlong Wei,Chao Zhang
The study examined the impact of varying molybdenum content (0 %, 5 %, and 10 % wt) on the high-temperature corrosion resistance of APS-sprayed Ni-clad Al coatings. The coatings were exposed to a simulated biomass-firing environment with pure KCl deposition at 600 °C for 168 h, and a mixed gas containing 15 % water vapor was passed through. The findings revealed that increasing Mo content improved the coating's high-temperature corrosion resistance. The coating with 10 % Mo displayed a continuous layer of Ni and Al oxides on its surface, while KCl was concentrated in the Mo region. Based on the experimental results, a mechanism for how the presence of Mo element contributes to the high-temperature corrosion resistance of NiAl coatings was proposed.
研究领域:
材料科学
·
腐蚀与防护
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）