ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Cheng Lin,Lianfu Jiang,Dawei Hu,Yiqun Li,Bo Cai,Jing Li,Yu Gu,Luyang Wang,Kan Zhang,Haibo Zeng
Although research progress on mimicking natural photosynthesis for solar-to-fuel conversion has been continuously made, exploring broadband spectral-responsive materials with suitable band positions and high stability still remains a huge challenge. Herein, we, for the first time, report novel AsP nanosheets (NSs) with P-type semiconducting property and enough negative conduction band, which can work as a stable near-infrared (NIR) region-responsive electron donor for water reductive hydrogen (H2) production. To mimic photosystem I, Au nanorods (NRs) act as electron transport media, which are also responsible for the enhanced electric field nearby, and 1T-MoS2 NSs as a hydrogen evolution catalyst are orderly coupled with AsP NSs with a sheet-rod-sheet structure by electrostatic self-assembly. The cascaded band level alignment enables unidirectional electron flow from AsP to Au and then to MoS2, and the optimum H2 production rate of the MoS2-Au-AsP ternary heterojunction reaches 125.52 μmol g–1 h–1 with good stability even after being stored for several months under light irradiation with a wavelength longer than 700 nm. This work provides a platform that is energetically tailored to drive a solar broad-spectrum fuel generation, including CO2 reduction and N2 fixation.
研究领域:
材料科学
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能源化学
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光催化
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产品关联
DOI
PubMed
高影响因子
POLYMER BULLETIN
·
2023
·Vol. 80
·期 12
·12865-12879
·
IF 3.2
Zhang Dawei,Wang Jiaming,Ren Lu,Meng Xiangchun,Luan Boyi,Zhang Yumin
The existence of fine oil droplets that are difficult to coalesce greatly reduces the oil–water separation efficiency of alkali‒surfactant‒polymer (ASP) flooding-produced water. In order to improve the oil–water separation efficiency and promote the reinjection of ASP-produced water, a novel cationic graft copolymer CDC was obtained by ultrasonically assisted copolymerization of chitosan and dimethyl diallyl ammonium chloride under the initiation of cerium ions. The copolymer CDC was characterized by FT-IR, XRD, TGA and SEM, and it was confirmed that the grafting reaction proceeded successfully. The flocculation effect of copolymer CDC on ASP-produced water (Nan 4–8, pH = 7.83; Sanyuan-6, pH = 9.95) was investigated. Copolymer CDC has better oil removal rate (90.5%) and light transmittance (95.25%) to Nan 4–8 produced water compared with Sanyuan-6-produced water. It has a better oil removal effect when the initial pH of produced water is 6–7 and the temperature is 30 °C. After the addition of CDC, the large-diameter oil droplets in produced water will coalesce and separate. It may be that under weak acid or neutral conditions, the electrostatic attraction of produced water is enhanced, compressing the double electron layer of oil droplets, and destroying the stability of oil droplets. In addition, the hydrophobic effect of –CH=CH 2 in CDC molecules increases the hydrophobic association between molecules and increases the probability of oil droplet collision during the flocculation process.
研究领域:
环境科学
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化学工程
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石油工程
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DOI
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-9
·
IF 4.38
Yue Song,Sun Kexin,Li Siyuan,Liu Yi,Zhu Qihao,Chen Yiyu,Yuan Dong,Wen Tao,Ge Mingjian,Yu Qiubo
In this paper, we first synthesis three-dimensional jasmine-like Cu@L-aspartic acid(L-ASP) inorganic–organic hybrid nanoflowers to load palladium-platinum nanoparticles (Pd–Pt NPs) as the signal enhancer in order to quantify intracellular speckle-type POZ domain protein. Scanning electron microscope, fourier transform infrared, energy dispersive spectrometer, X-ray photoelectron spectroscopy analysis was used to characterize the newly synthesized materials. The newly formed Cu@L-Asp/Pd-PtNPs can catalyze the decomposition of hydrogen peroxide and exhibit excellent catalytic performance. When different concentration of speckle-type POZ domain protein is captured by speckle-type POZ domain protein antibody linked to the surface of Cu@L-Asp/Pd–Pt NPs, the current signal decreases with the increase concentration of speckle-type POZ domain protein. After optimization, the speckle-type POZ domain protein immunosensor exhibited a good linear response over a concentration range from 0.1–1 ng mL −1 with a low detection limit of 19 fg mL −1 . The proposed sensor demonstrates good stability within 28 days, acceptable reproducibility (RSD = 0.52%) and selectivity to the speckle-type POZ domain protein in the presence of possible interfering substances and has potential application for detecting other intracellular macromolecular substances.
研究领域:
分析化学
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生物化学
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纳米技术
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生物传感器
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Yonghai Liu,Pengfei Zhang,Peiyun Lei,Yige Jin,Haoting Yu,Xingde Zhang,Yonglan Pan,Chunyan Ou,Tingming Fu
The core to the treatment of gout is the elimination of pathologic crystal, monosodium urate monohydrate (MSUM). The primary treatment available is to gradually dissolve the “culprit crystals” by lowering the blood uric acid concentration with medications, which often takes a long time and in severe cases must still be treated surgically. Herein, we developed a dynamic bionic platform based on a hydrogel composite membrane (HCM) to screen the direct facilitated solubilization of MSUM crystals by small organic molecules in bionic saturated, or even supersaturated, solutions. The customized and biologically safe (NAGA/PEGDA/NIPAM) HCM, which is consistent with the main amino acid composition of articular cartilage, well mimics the entire process of organic molecules leading to the dissolution of MSUM crystals in the joint system. With the verifications of this platform, it is shown that l-aspartic acid (ASP) significantly promotes the dissolution of MSUM crystals not only in saturated but also in supersaturated solutions. Furthermore, a novel mechanism called “crane effect” was used to explain this “dissolution effect” of ASP on MSUM, which stems from the ability of ASP to lock onto the surface of MSUM crystals through hydrogen bonding by virtue of its two carboxyl groups , and simultaneously its amino group lifts the uric acid molecules from the surface of MSUM crystals by virtue of interactions of hydrogen bonding . The results of bulk crystallization , scanning electron microscopy (SEM), powder X-diffraction (PXRD), and density-functional theory (DFT) studies are quantitatively consistent with this hypothetical "crane effect" mechanism. Hence, this HCM-based functional platform could provide entirely novel ideas and methods for drug design and screening for the treatment of pathological crystal diseases of gout.
研究领域:
生物医学工程
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药物设计
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晶体病治疗
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DOI
高影响因子
JOURNAL OF BIOMATERIALS APPLICATIONS
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2017
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IF 2.31
Yufeng Guo,Haixing Xu,Yiping Li,Fengzheng Wu,Yixuan Li,Yun Bao,Xiumei Yan,Zhijun Huang,Peihu Xu
Graphene oxide (GO) modified with hyaluronic acid (HA) and Arg-gly-asp peptide (RGD) was designed as a dual-receptor targeting drug delivery system to enhance the specificity and efficiency of anticancer drug delivery. Firstly, GO-HA-RGD conjugate was characterized to reveal its structure and morphology. Whereafter, doxorubicin (Dox) as a model drug was loaded on GO-HA-RGD carrier, which displayed a high loading rate (72.9%, GO:Dox (w/w) = 1:1), pH-response and sustained drug release behavior. Cytotoxicity experiments showed that GO-HA-RGD possessed excellent biocompatibility towards SKOV-3 and HOSEpiC cells. Additionally, the GO-HA-RGD/Dox had a stronger cytotoxicity for SKOV-3 cells than either GO-HA/Dox (single receptor) or GO/Dox (no receptor). Moreover, celluar uptake studies illustrated that GO-HA-RGD conjugate could be effectively taken up by SKOV-3 cells via a synergic effect of CD44-HA and integrin-RGD mediated endocytosis. Hence, GO-HA-RGD nanocarrier is able to be a promising platform for targeted cancer therapeutic.
研究领域:
纳米技术
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药学
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癌症研究
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
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2022
·
IF 0
Lichao Chen,Bo Wang,Haohao Ren,Yanan Wu,Defu Lyu,Yanan Ouyang,Qiyi Zhang,Yonggang Yan
Poly-amino acid (PAA) is a promising biomaterial in biomedical engineering due to its similar amide bond structure to collagen and excellent biocompatibility, but the lack of osteogenic activity and inferior mechanical strength limit its long-term application in orthopedics. In this study, a poly-amino acid/poly (p-benzamide) (PAA-PBA) copolymer with high mechanical strength was designed and fabricated by the method of solution polymerization. The chain structures, thermal properties and mechanical properties of these polymers were evaluated and results showed that PBA greatly promoted the mechanical properties of PAA, and the copolymer performed the maximum mechanical strengths with compressive strength, bending strength and tensile strength of 123 MPa, 107 MPa and, 95 MPa, respectively. To increase the bioactivity of surface, a bioactive coating that consists of poly-(dopamine) (PDA) nanolayers and tripeptide Arginine-Glycine-Aspartic acid (RGD) on sulfonated PAA-PBA copolymer was created. A porous structure appeared on the surface after modification, the surface roughness and hydrophilicity of copolymer has been improved obviously after introducing PDA and RGD peptide coating. The in vitro bioactivity evaluation demonstrated that the RGD-functionalized sample showed a significantly improved ability to promote bone apatite mineralization, cell adhesion, proliferation and osteogenic differentiation. In a word, such a strategy of material synthesis and surface modification method shows a great potential for broadening the use of PAA in the application of load-bearing bone substitute biomaterials.
研究领域:
生物医学工程
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材料科学
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组织工程
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产品关联
DOI
PubMed
RSC Advances
·
2020
·Vol. 10
·期 32
·18918-18926
·
IF 3.119
Mengjin Zhai,Mian Wu,Cunying Wang,Xiaobing Li
The existence of fine-sized oil drops that are difficult to coalesce greatly decreases the separation efficiency of produced water from alkali, surfactant, and polymer flooding technology (ASP) containing oil-in-water emulsions. To improve oil–water separation efficiency, a silica-supported polyether polysiloxane quaternary ammonium (abbreviated as PPQA@SiO2) demulsifier was synthesized. The supported demulsifier possesses a rough surface structure and large surface area. In addition, it displays high thermal stability. It was applied for treating the produced water from ASP flooding. The effects of dosage, treatment temperature and treatment time on the oil removal efficiency from ASP produced water were investigated. Owing to the synergetic effect of demulsification and adsorption, the supported demulsifier exhibited an oil removal greater than 92% within 50 min at the initial oil concentration of 300 mg L−1, which is much higher than that of a commercial demulsifier SA001 (40.33%). Furthermore, the demulsification mechanism was explored from the perspective of the zeta potential, mean diameter and size distribution of the oil droplets. The high oil removal efficiency establishes PPQA@SiO2 as a promising candidate for oil–water separation from the ASP flooding produced wastewater.
研究领域:
环境科学
·
化学工程
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DOI
PubMed
高影响因子
Materials Today Communications
·
2022
·
IF 3.662
Xinyu Xu,Hao Wang,Shuling Zhang,Xiaohan Mei,Boda Ying,Ruiyan Li,Yanguo Qin
High-performance polymers, such as polyetherimide (PEI), are promising bone biomaterials that meet the mechanical demands of bone defect repair. To achieve better therapeutic outcomes, the osteoinduction of high-performance polymers requires further improvement. In this study, the fused deposition modeling (FDM) 3D printing method was applied to fabricate orthogonal porous PEI scaffolds. Inspired by the simulation of the extracellular matrix (ECM), The polydopamine (PDA) and Arg-Gly-Asp (RGD) peptides were applied to construct an osteogenic microenvironment on PEI/PDA/RGD scaffold. The characterization, bioactivity, and osteogenic differentiation of the scaffolds were systematically investigated. The mechanical properties of the PEI/PDA/RGD scaffold were similar to those of bone tissues with favorable hydrophilicity and no biotoxicity. The adhesion, proliferation, extracellular bone matrix formation, calcium deposition, and alkaline phosphatase (ALP) vitality of MC3T3-E1 osteoblasts was higher in the PEI/PDA/RGD scaffold compared with other scaffolds. The PEI/PDA/RGD scaffold promoted the osteogenic differentiation of MC3T3-E1 osteoblasts by upregulating their gene expression of osteopontin (OPN), runt-related transcription factor 2 (RUNX-2), bone morphogenetic protein (BMP-2), osteocalcin (OCN), ALP, and especially type I collagen (COL-1). The PEI/PDA/RGD scaffold fabricated in this study is a promising bone biomaterial with appropriate mechanical properties and ideal osteogenic microenvironment, which has potential application in the field of bone defect repair. The construction of osteogenic microenvironment on 3D printed porous PEI scaffold could broaden the application of polyetherimide in bone biomaterials.
研究领域:
生物材料
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组织工程
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生物医学工程
·
细胞生物学
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产品关联
DOI
高影响因子
Journal of Cleaner Production
·
2023
·
IF 11.1
Shuzhen Wang,Jiaye Wang,Yuan Yao,Jia-Yan Liu,Yankun Gao,Qiaoli Hua,Qingcai Jiao,Junzhong Liu,Yang Jin,Hongjuan Zhang,Lina Chen
The strategies based on enzyme immobilization into/on metal-organic frameworks (MOFs) have been widely developed, but they still face some challenges in practical applications, such as limited recycle times, low loading efficiency. In this work, aspartate aminotransferase (AspAT), branched-chain aminotransferase (BCAT), polyvinyl pyrrolidone/aspartate (PA), cobalt ions and sodium 2-amino terephthalate were one-pot mixed in water at room temperature, and self-assembled to encapsulate the enzymes into cobalt-amino terephthalate framework (CAF) in 15 min. Compared with free enzymes , the biocomposite (A&B& [email protected] ) exhibited superior performances such as high encapsulation efficiency (55%), reusability (≥12), and resistance capacity to harsh conditions. Meanwhile, the activity recovery (A R ) value (59.4%) of the biocomposite without adding polyvinyl pyrrolidone and aspartate was almost half of that (108.2%) of A&B& [email protected] , and this enhancement was further demonstrated to be from Asp action via 1 H-NMR and UV–Vis. To investigate the general applicability of this approach, the other aminases were also de novo immobilized with all their A R values reached more than 117%, even 180% for ω-transaminase. This study provided a sustainable and green approach for clean production of the significant chiral amine compounds.
研究领域:
化学工程
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生物化学
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材料科学
·
催化
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Zhengwei Xu,Xiaohong Jiang
TiO 2 nano-porous arrays with appropriate structural parameters, roughness and wettability have application potential in implantable titanium-based (Ti-based) alloys. In this research, the polydopamine (PDA) and Arg-Gly-Asp (RGD) peptide are adopted to construct a favorable osteogenic microenvironment on the anodized TiO 2 nano-porous arrays before and after heat treatment. The characterization of surface morphologies and chemical compositions has been conducted to confirm the feasibility of the PDA-based modification and RGD peptide immobilization. Via immersion in the modified-simulated body fluid (m-SBF) for 7 days, the PDA-based modified samples have represented enhanced biomineralization performance. Moreover, the cell adhesion and proliferation assay, alkaline phosphatase (ALP) activity assay and quantitative real-time polymerase chain reaction (qRT-PCR) assay in vitro have indicated that the heat-treated TiO 2 nano-porous arrays modified with PDA and RGD peptide have endowed the seeded MC3T3-E1 mouse pre-osteoblasts with excellent osteogenic performance. Such modified TiO 2 nano-porous arrays have great latent capacity in implantable materials for biomineralization and osteogenesis.
研究领域:
生物材料
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生物医学工程
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组织工程
·
材料科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Yinshuo Xu,Yuanjie Shan,Yuta Nishio,Kenichiro Todoroki,Toshimasa Toyo'oka,Toufeng Jin,Jun Zhe Min
With the development of untargeted metabolomics, studies have shown that alterations in the serum levels of specific amino acids are strongly correlated with the development of early-stage colorectal cancer (CRC). However, the relationship between optically active free amino acids in human nails and the risk of CRC remains unclear. In this study, a highly sensitive UHPLC-MS/MS chiral separation method based on ( S )-(−)-DBD-Pro-COCl derivatization was established for the simultaneous detection of DL-Asp, DL-Kyn, DL-α-hydroxybutyric acid (DL-α-HB), and Cystamine in the fingernails of patients with CRC. The derivatization reagents were incubated with DL-amino acids, DL-α-HB and Cyst at 60 °C for 15 min to form diastereomers. Subsequently, an Imtakt cadenza CD-C 18 column (3.0 × 150 mm, 3.0 μm) was used for the separation of diastereomers under gradient elution of 10 mM HCOONH 4 in H 2 O as mobile phase A and 0.1 % HCOOH in CH 3 CN as mobile phase B. The degrees of separation (Rs) ranged from 1.76 to 3.21. R 2 was >0.9995, indicating good linearity; the limit of detection (LOD) was 25–100 fmol; the interday and intraday precision of DL-Asp and DL-α-HB detection were 0.85 %–4.91 %; and the average recovery ranged from 81.12 % to 110.2 %. However, during the determination process of fingernail samples, only DL-Asp and DL-α-HB were successfully detected, while DL-Kyn and Cyst were not detected. Therefore, this novel method was used to quantify DL-Asp and DL-α-HB in fingernail samples from 32 healthy volunteers (HVs) and 16 patients with CRC (CPs). The results revealed significant differences in D-Asp ( p < 0.05), L-Asp ( p < 0.01), and L-α-HB ( p < 0.05) levels between male HVs and CPs and in D-α-HB ( p < 0.05) levels between female HVs and CPs. In addition, the D/L-α-HB ratio ( p < 0.01) was significantly different between male HVs and CPs. These findings validate the use of DL-amino acids, DL-α-HB and Cyst from human fingernails as potential diagnostic biomarkers for CRC, providing a basis for the clinical application of human fingernail samples for the noninvasive diagnosis of CRC.
研究领域:
分析化学
·
癌症研究
·
代谢组学
·
临床诊断
·
手性分离
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DOI
PubMed
高影响因子
Acta Metallurgica Sinica-English Letters
·
2017
·Vol. 30
·期 6
·558-566
·
IF 1.292
Cao Sheng,Li He-Jun,Li Ke-Zhi,Lu Jin-Hua,Zhang Lei-Lei
Carbon/carbon (C/C) composites were deposited with graphite-like carbon (GLC) coating, and then, Arg-Gly-Asp acid (RGD) peptides were successfully immobilized onto the functionalized GLC coating. GLC coating was utilized to prevent carbon particles releasing and create a uniform surface condition for C/C composites. RGD peptides were utilized to improve biocompatibility of GLC coating. Surface chemical characterizations of functionalized GLC coating were detected by contact angle measurement, X-ray photoelectron spectroscopy and Raman spectra. Optical morphology of GLC coatings was observed by confocal laser scanning microscopy. In vitro biological performance was determined using samples seeded with MC3T3-E1 osteoblast-like cells and cultured for 1 week. Surface characterizations and morphological analysis indicated that C/C composites were covered by a dense and uniform GLC coating. Contact angle of GLC coating was reduced to 27.2° when it was functionalized by H 2 O 2 oxidation at 40 °C for 1 h. In vitro cytological test showed that the RGD peptides immobilized GLC coating had a significant improvement in biocompatibility. It was suggested that RGD peptides provided GLC coating with a bioactive surface to improve cell adhesion and proliferation on C/C composites.
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DOI
高影响因子
危险品化学品经营许可证（带存储）