MOLECULES
·
2024
·Vol. 29
·期 18
·4490
·
IF 4.2
Yuxia Li,Jingxuan Ren,Zeting Meng,Baozhu Zhang
In this study, a turn-on fluorescence sensor for the detection of adenosine 5′-triphosphate (ATP) was developed and tested using ATP-DNA2-Ag NCs. The results showed that the fluorescence of ATP-DNA2-Ag NCs was significantly enhanced with the addition of ATP. The fluorescence enhancement was a result of the specific binding activity of the ATP aptamer and ATP, which caused G-rich sequences to approach the dark DNA-Ag NCs, owing to the alteration in ATP aptamer conformation. The proposed sensor demonstrated a good linear range of 18–42 mM and a limit of detection (LOD) of 2.8 μM. The sensor’s features include sensitivity, selectivity, and simple operation. In addition, the proposed sensor successfully measured ATP in 100-fold diluted fetal bovine serum.
研究领域:
分析化学
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纳米技术
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生物传感器
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生物医学工程
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Zhou Jiang,Erhu Xiong,Songlan Pan,Jianhua Chen,Huihuang Yi,Yinfeng Li,Yi Qing,Zhen Zou
Abundant adenosine triphosphate (ATP), an important mediator of metabolic reprogramming in cancer progression, is regarded as a significant focus in cancer treatment. Nonetheless, due to low selectivity, the attempts of ATP exhaustion may induce unacceptable side effects because ATP also plays key roles in maintaining normal cell function. Inspired by feedback inhibition in nature, here, we propose feedback inhibition of mitochondrial ATP synthetic pathway for tumors inhibition while minimizing side effects. As a proof-of-concept, an ATP-responsive ZIF-90 as a broad framework for mitochondrial-targeted delivery of 2, 2’-azobis[2-(2-imidazolin-2-yl)propane]-dihydrochloride (AIPH) and an FDA-approved drug bedaquiline (BE). It was found that ZIF-90/AIPH/BE nanocomplex exhibits unique properties of high pulmonary accumulation and mitochondria-targeting capability. When ATP is present, the ZIF-90/AIPH/BE nanoparticles were eroded and release the wrapped contents because of the competitive binding between ATP and the Zn2+ in ZIF-90. The released AIPH and BE significantly reduced ATP-production, causing mitochondrial ATP depletion. The reduced ATP restricted the follow-up release of ZIF-90/AIPH/BE nanocomplex in a negative-feedback manner. The feedback inhibition mechanism expands the possibilities for targeted disease treatment and opens up new avenues for ATP-based nanomedicine.
研究领域:
生物化学
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肿瘤学
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药理学
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生物材料
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DOI
PubMed
高影响因子
SENSORS
·
2018
·Vol. 18
·期 10
·3281
·
IF 2.475
Jingjing Zhang,Chunzheng Yang,Chaoqun Niu,Chen Liu,Xuepin Cai,Jie Du,Yong Chen
In this study, a label-free fluorescent, enzyme-free, simple, highly sensitive AND logic gate aptasensor was developed for the detection of adenosine triphosphate (ATP). Double-stranded deoxyribonucleic acid (DNA) with cohesive ends was attached to graphene oxide (GO) to form an aptasensor probe. ATP and single-stranded DNA were used as input signals. Fluorescence intensity of PicoGreen dye was used as an output signal. The biosensor-related performances, including the logic gate construction, reaction time, linearity, sensitivity, and specificity, were investigated and the results showed that an AND logic gate was successfully constructed. The ATP detection range was found to be 20 to 400 nM (R2= 0.9943) with limit of detection (LOD) of 142.6 pM, and the sensitivity range was 1.846 × 106to 2.988 × 106M−1. This method for the detection of ATP has the characteristics of being simple, low cost, and highly sensitive.Keywords:ATP detection;label-free fluorescence;enzyme-free;graphene oxide;logic gate
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
Biomed Research International
·
2018
·
IF 2.583
Xuemei Ye,Aifang Huang,Xianqin Wang,Congcong Wen,Lufeng Hu,Guanyang Lin
Linezolid has been widely used in serious infections for its effective inhibiting effect against multidrug-resistant gram-positive pathogens. However, linezolid caused severe adverse reactions, such as thrombocytopenia, anaemia, optic neuropathy, and near-fatal serotonin syndrome. In order to investigate the toxicity of linezolid, twenty-four Sprague-Dawley rats were randomly divided into: control group (n=7), low-group (n=8), and high-group (n=9). The rats of low-group and high-group were given by gavage with linezolid 60 and 120 mg/kg/day for 7 days, respectively. The serum concentration of linezolid was determined by high performance liquid chromatography (HPLC); blood metabolic change was analyzed by gas chromatography-mass spectrometer (GC-MS). Adenosine triphosphate (ATP) concentration in HepG2-C3A after being cultured with linezolid was determined by HPLC. The results showed that there were six metabolites and nine metabolites had statistical differences in low-group and high-group (P<0.05). The trimethyl phosphate was the most significant indicator in those changed metabolites. Except for d-glucose which was slightly increased in low-group, octadecanoic acid, cholest-5-ene, hexadecanoic acid, α-linolenic acid, eicosapentaenoic acid, 9,12-Octadecadienoic acid, and docosahexaenoic acid were all decreased in low-group and high-group. ATP concentration was decreased in HepG2-C3A after cultured with linezolid. In conclusion, the toxicity of linezolid is related to its serum concentration. Linezolid may inhibit the synthesis of ATP and fatty acid.
研究领域:
药理学
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毒理学
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代谢研究
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Xiaojiao Dai,Ziling Huang,Chuanyi Liu,Zhigang Li,Qingmin Chen,Yi Xu,Xiaomei Chen,Quansheng Chen,Jie Wei
Adenosine triphosphate (ATP) levels are recognized as a critical indicator of microbial content in food safety. The development of highly efficient ATP-sensing methods is essential for assessing food freshness. Herein, we propose a simple-yet-efficient hemin/G-quadruplex (G4) DNAzyme-based aptasensor for ATP detection and oyster freshness assessment. The anti-ATP aptamer subunits specifically bind to ATP, leading to self-assembly of the hemin/G4 DNAzyme. The newly produced DNAzyme then catalyzes the oxidation of ABTS 2- to produce the colored ABTS •− , providing an absorbance response for sensitive ATP analysis. The aptasensor facilitates high-performance and accurate determination of ATP with a low detection limit of 0.065 μM. Furthermore, this sensor enables reliable ATP analysis in oyster samples and evaluation of oyster freshness. In particular, a good linear correlation was established between microbial content and ATP levels. The proposed aptasensor for the assessment of freshness is characterized by its simplicity and efficiency, potentially offering a new tool to monitor food safety.
研究领域:
生物化学
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食品安全
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分子生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Cunduo Tang,Taisong Shen,Xueyang Bai,Yao Wang,Jiashu Zhang,Yunchao Kan,Dandan Li,Lunguang Yao,Hongling Shi
Nicotinamide mononucleotide (NMN) is a very important bioactive nucleotide that is of great help to human health. However, its widespread application has been limited by its high production costs, especially the cost of the core substrates, coenzyme, and enzymes. In this study, the ADP/GDP-polyphosphate phosphotransferase RhPPK2 originating from Rhodobacter sphaeroides was successfully expressed in Escherichia coli with high-level solubility, and the enzyme activity in the lysate supernatant reached 21.9 ± 0.65 U/mL. And then, the temperature profiles, pH profiles, and kinetic parameters of purified reRhPPK2 were systematically characterized, which demonstrate its potential for application in enzymatic ATP regeneration systems. Furthermore, the introduction of reRhPPK2 for ATP regeneration significantly enhanced NMN production efficiency, achieving a 2.3-fold increase compared to the conventional ATP supplementation method. Finally, the production efficiency of NMN was further improved by a single-factor experiment and L9(34) orthogonal design, and the yield was up to 14.6 ± 0.51 g/L, about 5.4 times of the initial yield. This research substantially reduced NMN production costs and established a robust foundation for industrial-scale NMN production.
研究领域:
生物化学
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合成生物学
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酶工程
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2021
·Vol. 32
·期 23
·27871-27881
·
IF 2.478
Zhang Hongda,Zhou Hong,Liu Guolong,Ge Jing,Jin Liguo
The hybrid fillers (Al 2 O 3 –ATP hybrids) composed of two types of inorganic materials—modified alumina (Al 2 O 3 ) and modified attapulgite (ATP)—were principally prepared, following which the epoxy matrix (EP) composites reinforced with Al 2 O 3 –ATP hybrids were synthesized. The mechanical and dynamic mechanical properties of fabricated Al 2 O 3 –ATP/EP composites are enhanced by doping Al 2 O 3 –ATP hybrids into EP. The highest impact and flexural strength of EP composites (3 wt% Al 2 O 3 –3 wt% ATP/EP) are improved to 27.88 kJ/m 2 and 157.04 MPa, which are 457.6% and 89.2% higher than those of the pure epoxy resin, respectively. The glass transition temperature of Al 2 O 3 –ATP/EP composites increases by 8.8 °C, compared with pure EP composites and the dielectric constant and dielectric loss can reach 3.19 and 0.0041 at 100 Hz. These excellent mechanical and dynamic mechanical properties, accompanied by dielectric property may expand the practical applications of EP composites in industrial engineering.
研究领域:
材料科学
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工程学
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DOI
高影响因子
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Baolin Li,Yaxin Huang,Zixin Zhu,Min Zhong,Guangrong Li,Jinbo Liu
Adenosine triphosphate (ATP) plays a critical role in cellular energy metabolism, acting as the energy source for many biological reactions. Abnormal fluctuations in ATP levels are directly linked to various diseases such as malignancies, bacterial infections, and cardiovascular disorders, making early-stage disease detection through rapid, highly sensitive ATP measurement essential. The current methods for ATP detection suffer from drawbacks such as complex procedures, reliance on expensive instruments, and prolonged reaction times. To this end, we have developed a novel biosensor named ATP Output Sensor Activated by CRISPR (AOSAC).This biosensor employs aptamer-based specific recognition combined with a dual-enzyme, amplification-free system involving Exo III and CRISPR-Cas12a for ATP detection. This sensor operates through an aptamer in a closed duplex structure, upon binding ATP, releases a trigger strand. This strand displaces an activation chain, which in turn interacts with Cas12a's crRNA, initiating trans-cleavage activity that cleaves a single-stranded DNA probe to produce a fluorescent signal. Concurrently, the activation chain's release sites are targeted by Exo III, enhancing signal amplification without the need for complex nucleic acid amplification, thus providing a highly selective, rapid detection method. This technique offers a linear detection range from 0 nM to 20 μM with a detection limit of 44.2 nM, presenting a novel strategy for signal amplification and high specificity in distinguishing ATP from its analogues.
研究领域:
分子生物学
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生物化学
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医学检测
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2019
·Vol. 29
·期 39
·1904144
·
IF 15.621
Zhanwei Zhou,Yadong Liu,Minghua Zhang,Chenzi Li,Ruoxi Yang,Jing Li,Chenggen Qian,Minjie Sun
Protein-based theranostic agents (PBTAs) exhibit superior performance in the diagnosis and therapy of cancers. However, the in vivo applications of PBTA are largely limited by undesired accumulation, penetration, or selectivity. Here, an ATP-supersensitive protein cluster is fabricated for promoting PBTA delivery and enhancing magnetic resonance imaging (MRI)-guided tumor photothermal therapy. Gd 3+ - and CuS-coloaded small bovine serum albumin nanoparticles (GdCuB) are synthesized as the model protein with a size of 9 nm and are encapsulated into charge switchable polycations (DEP) to form DEP/GdCuB nanoclusters of 120 nm. In blood circulation, DEP/GdCuB significantly extends the half-lifetime and thereby enhances the tumor accumulation of GdCuB. When the clusters reach the tumor site, the extracellular adenosine triphosphate (ATP) can effectively trigger the release of GdCuB, resulting in tumoral deep penetration as well as the activation of T 1 -weighted MRI ( r 1 value switched from 2.8 × 10 −3 to 11.8 × 10 −3 m −1 s −1 ). Furthermore, this delivery strategy also improves the tumoral photothermal therapy efficacy with the MRI-guided therapy. The study of ATP-activated nanoclusters develops a novel strategy for tumor deep penetration and on/off imaging of PBTA by size switchable technology, and reveals the potential for MRI-guided therapy of cancers.
研究领域:
纳米技术
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生物医学工程
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癌症治疗
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磁共振成像
·
药物递送系统
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 21
·3832-3841
·
IF 4.872
Guan-Hai Wang,Guo-Liang Huang,Yi Zhao,Xing-Xiang Pu,Tong Li,Jun-Jie Deng,Jian-Tao Lin
Stimuli-responsive nanocarriers for anticancer drug and gene co-delivery are a promising strategy in cancer therapy due to their combination of chemotherapy and gene therapy. In this work, we developed a facile and effective method to fabricate stimuli-responsive nanocarriers for anticancer drug and gene co-delivery based on complexes of polyethylenimine (PEI) with an adenosine triphosphate (ATP) responsive aptamer duplex (ARAD). No chemical reactions or complex modifications were used in the construction processes. In this system, Doxorubicin-loaded aptamer duplex and plasmid DNA (p53) can be bound by PEI by electronic interactions to form stable complexes which effectively protect the aptamer and p53 from DNase degradation. The intercalated Dox can be released on-demand by a structural change in the aptamer duplex in an ATP-rich environment. The morphology and average size of the nanocarriers were characterized by zeta potential and transmission electron microscopy (TEM). The nanocarriers exhibit lower cell toxicity in HeLa cell lines relative to PEI. RT-PCR and Western blot analysis confirmed that p53 could be effectively delivered and expressed in HeLa cells by PEI/ARAD/p53 complexes. Moreover, the apoptosis percentage of HeLa cells treated with PEI/ARAD/Dox/p53 complex increased to 40.8%, compared to 24.7% for PEI/ARAD/Dox complex and 11.5% for PEI/ARAD/p53, respectively. The result demonstrated that the combinatorial delivery of Dox and p53 by nanocarriers could induce synergistic actions and lead to effective cancer cell apoptosis.
研究领域:
癌症治疗
·
药物递送系统
·
分子生物学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Wei Wan,Meijia Liu,Xiangling Ren,Longfei Tan,Changhui Fu,Qiong Wu,Jun Ren,Hongbo Li,Zhongbing Huang,Xianwei Meng
Adenosine triphosphate (ATP) actively involve into cellular processes. Abnormal levels of ATP related to a variety of diseases, therefore determining the ATP level is of pivotal significance for diagnoses. Here, a highly sensitive fluorescence probe based on gold nanoclusters (Au NCs) was constructed for the detection of ATP, and an expanded detection range and low limit of detection (LOD) were realized in our work. Zeolitic imidazolate framework-8 (ZIF-8) enhanced the fluorescence intensity of Au NCs by the encapsulation and less ATP cause to a stronger fluorescence change, which guaranteed detection reproducibility and facilitated lower LOD. The LOD of Au NCs encapsulated ZIF-8 (AZ) for ATP detection was expanded to 2 μM, which was only 0.05 % of sole Au NCs (4000 μM). Significantly, ATP of high concentration quenched AZ by disrupting the structure of ZIF-8 which expanded the detection range. Moreover, the linear range of high concentration ATP detection in serum was 600–4000 μM, which was closed to the normal level of ATP in physiological. Therefore, due to its low LOD and high sensitivity, AZ can be applied for fluorescence detection and initial diagnosis of disease related to ATP.
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Shicai Xu,Chao Zhang,Shouzhen Jiang,Guodong Hu,Xiaoyue Li,Yan Zou,Hanping Liu,Jun Li,Zhenhua Li,Xiaoxin Wang,Mingzhen Li,Jihua Wang
As the major energy molecule of cells, adenosine triphosphate (ATP) regulates various biological processes and has been found to be closely related to many diseases. Therefore, ATP detection in trace amounts is very useful for understanding various biological processes, studying cellular events such as proliferation and apoptosis, and estimating contaminated degree of food and medical instrument. To date, the trace sensing ATP at picomolar level in biological systems is still a major challenge. Because of unique electrical and structural properties, graphene has attracted much attention in biosensing applications. Here, a sensitive and selective graphene foam field-effect transistor (GF-FET) biosensor for ATP detection is demonstrated. The lowest detection limit of the biosensors for analyzing ATP is down to 0.5 pM, which is one or several orders lower than the reported results. Moreover, the GF-FET biosensor show a good linear current response to ATP concentrations in a broad range from 0.5 pM to 50 μM. The GF-FET sensor surface can be regenerated for many times and used for up to weeks without significant loss of functionality. Based on this sensing platform, label-free measurements of ATP concentrations in human serum as well as in cell lysate are demonstrated. The work may provide a novel platform to study ATP release and energy-regulated biological processes, suggesting a promising future for biosensing applications.
研究领域:
生物传感器
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材料科学
·
医学诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）