SEPARATION AND PURIFICATION TECHNOLOGY
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2022
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IF 9.136
Yun Cheng,Xiaobin Zhao,Qiong Zhang,Xueqin Li,Zhong Wei
Acteoside (ACT) is an active component of Cistanche tubulosa with rare content and complex structure. The development of ACT-based molecularly imprinted hybrid membranes (A-MIHMs) with high rebinding capabilities and selectivity remains a challenge to obtain high purity of ACT. Here a series of A-MIHMs was designed for highly selective separation of ACT. The A-MIHMs were fabricated by incorporating imprinted nanofillers made of ACT-imprinted surface molecularly imprinted polymers and polyethylenimine modified mesoporous silica (SMIP-PEI/MCM-41) into polyvinylidene fluoride (PVDF) powders. The as-prepared optimum A-MIHMs-400 exhibited the highest rebinding capabilities of 96.24 mg/g, an excellent rebinding selectivity of 4.48, and permselectivity of 6.37 for the ACT. The following reasons were attributed to the results: first, the incorporated SMIP-PEI/MCM-41 nanofillers were distributed homogeneously into the A-MIHMs, and the pores of the membrane were tailored to form the interconnected imprinted reticular structure, and it can capture abundant ACT, resulting in highly selective separation of ACT. Second, the formed imprinted cavities and sites on the imprinted reticular structure can uniquely recognize ACT, enhancing A-MIHMs selectivity for ACT. Third, the amino groups of PEI acted as affinity sites with a high-affinity for the hydrophilic molecule of ACT, allowing A-MIHMs to bind the ACT with high rebinding capabilities and selectivity. After eight cycles, the regeneration rate approached 90%, indicating that A-MIHMs were highly renewable. The design of MIHMs with an imprinted reticular structure would provide a new strategy for the highly selective separation of active components from natural products.
研究领域:
材料科学
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化学工程
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药学
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分离技术
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
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2026
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IF 5.4
Baohong Zhao,Yaqian Ping,Xiaohui Dou,Ziying Jiang,Fangxu Yin,Daqing Sun
Background Slow transit constipation (STC) is characterized by delayed intestinal transit and impaired defecation, with enteric nervous system (ENS) injury and inflammatory dysregulation contributing to its pathogenesis. Acteoside (ACT), a natural phenylethanoid glycoside, has anti-inflammatory and neuroprotective properties. However, its therapeutic effect and mechanism in STC remain unclear. Methods The effects of ACT were investigated in a loperamide (LOP)-induced STC-like mouse model. Intestinal motility, colonic morphology, and ENS integrity were evaluated by functional assays, hematoxylin and eosin (H&E) staining, and immunofluorescence. Transcriptomic analysis was performed to screen ACT-responsive inflammatory pathways. NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation and cytokine production were assessed by Western blotting, enzyme-linked immunosorbent assay (ELISA), and quantitative reverse transcription polymerase chain reaction (qRT-PCR). Nlrp3 -knockout ( Nlrp3 -KO) mice were used for genetic validation. Results ACT shortened defecation latency, increased fecal water content and pellet number, and improved intestinal propulsion. ACT also alleviated colonic histopathological injury, restored muscular layer thickness, preserved HuC/D-positive myenteric neurons, and increased PGP9.5-positive nerve fiber density. Transcriptomic analysis identified NLRP3-related inflammatory signaling within broader high-dose ACT-responsive transcriptional changes. At the molecular level, high-dose ACT reduced the protein levels of NLRP3, cleaved-caspase-1, and cleaved-interleukin-1β (IL-1β) expression and reduced IL-1β, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) production. Nlrp3 deficiency partially reproduced ACT-mediated protection against STC-like phenotypes, inflammasome activation, and ENS injury, whereas ACT retained additional protective effects in Nlrp3 -KO mice, suggesting that NLRP3-independent mechanisms may also contribute. Conclusion ACT alleviates LOP-induced STC-like injury, and this protection is associated with attenuation of NLRP3 inflammasome activation and preservation of ENS integrity, while additional NLRP3-independent mechanisms may also contribute.
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DOI
PubMed
高影响因子
Cellular and Molecular Bioengineering
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2025
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IF 5
Liu Junyu,Wang Hua,Zhang Caihua
Background Skin wound healing is a complex biological process involving cellular, molecular, and physiological events. Traditional treatments often fail to provide optimal outcomes, particularly for chronic wounds. Objectives This study aimed to develop a self-healing hydrogel loaded with Acteoside, a bioactive compound with antioxidant and anti-inflammatory properties, to enhance skin wound healing. Methods Using transcriptomic analysis, Rab31 was identified as a key target of Acteoside in regulating hair follicle stem cells (HFSCs). In vitro assays demonstrated that Acteoside promotes HFSC proliferation, migration, and differentiation by upregulating Rab31 expression. The self-healing hydrogel was prepared using quaternized chitosan derivatives, which exhibited excellent mechanical properties, antibacterial, and antioxidant activities. Results In vivo studies in a mouse model showed that Acteoside-loaded hydrogel significantly accelerated wound healing, promoting skin regeneration and improving wound closure. Conclusions This research highlights the potential of Acteoside-loaded self-healing hydrogels as an innovative therapeutic strategy for enhancing skin wound healing. By modulating HFSC activity, this hydrogel offers a promising solution for improving healing outcomes in challenging wound environments. Graphical Schematic representation of an injectable self-healing hydrogel loaded with the phenylethanoid compound acteoside for regulating the proliferation and differentiation of HFSCs to mediate the healing of skin wounds.
研究领域:
生物材料学
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分子生物学
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皮肤修复
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产品关联
DOI
PubMed
高影响因子
Molecular Medicine Reports
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2022
·Vol. 26
·期 6
·1-11
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IF 3.423
Wenjuan Guo,Xiaodi Wang,Fang Liu,Shuo Chen,Shuai Wang,Qingrui Zhang,Lan Yuan,Shiyu Du
Ulcerative colitis (UC) is a significant burden on human health, and the elucidation of the mechanism by which it develops has potential for the prevention and treatment of UC. It has been reported that acteoside (ACT) exhibits strong anti‑inflammatory activity. In the present study, it was hypothesized that ACT may exert a protective effect against UC. The effects of ACT on inflammation, oxidative stress and apoptosis were evaluated using dextran sulphate sodium (DSS)‑treated mice and DSS‑treated human colorectal adenocarcinoma Caco‑2 cells, which have an epithelial morphology. The results demonstrated that the ACT‑treated mice with DSS‑induced UC exhibited significantly reduced colon inflammation, as demonstrated by a reversal in body weight loss, colon shortening, disease activity index score, inflammation, oxidative stress and colonic barrier dysfunction. Further in vivo experiments demonstrated that ACT inhibited DSS‑induced apoptosis in colon tissues, as demonstrated by the results of the TUNEL assay and the altered protein expression levels of Bax, cleaved caspase‑3 and Bcl‑2. Furthermore, DSS significantly stimulated the protein expression levels of high mobility group box 1 protein (HMGB1), which serves a central role in the initiation and progression of UC, an effect which was markedly inhibited by ACT. Finally, DSS significantly decreased the protein expression levels of heme oxygenase‑1 (HO‑1) in colon tissues and the effect of ACT on GSH, apoptotic proteins and HMGB1 was markedly attenuated in the presence of the HO‑1 inhibitor tin protoporphyrin. In conclusion, ACT ameliorated colon inflammation through HMGB1 inhibition in a HO‑1‑dependent manner.
研究领域:
药理学
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免疫学
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胃肠病学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
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IF 6.165
Helin Xu,Xueqin Li,Yanyan Hao,Xiaobin Zhao,Yun Cheng,Jinli Zhang
To achieve simple and efficient separation of acteoside (ACT), extraction ACT from crude extracts of Cistanche tubulosa via ionic liquid (IL) based aqueous two-phase system (ATPS) is investigated. The IL–ATPS comprising [C 4 mim]BF 4 and (NH 4 ) 2 SO 4 exhibits excellent extraction performance, extracting almost all ACT in the [C 4 mim]BF 4 -rich phase. The high polarity of [C 4 mim]BF 4 having multi-hydrogen bond receptors is conducive for extracting ACT in the IL-rich phase, and (NH 4 ) 2 SO 4 not only provides a weak acidic microenvironment for increasing ACT stability in the IL-rich phase but also reduces the solubility of ACT in the salt-rich phase via the salting-out effect. In addition, molecular simulation results show that ACT is extracted in the [C 4 mim]BF 4 -rich phase via multi-interactions, including hydrogen bonding, van der Waals forces, and π–π stacking. This study is expected to provide a valuable reference for the separation of bioactive constituents from natural products.
研究领域:
植物化学
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天然产物分离
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DOI
高影响因子
CHEMICAL ENGINEERING RESEARCH & DESIGN
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2022
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IF 4.119
Chunying Li,Fang Nie,Changyin Feng,Mengfei Tian,Meiting Yu,Chunjian Zhao,Yujie Fu
A creative dual-template molecularly imprinted polymer (Fe 3 O 4 @MCM-41-MIPs) with good magnetic properties and specific recognition of echinacoside and acteoside was prepared by surface imprinting. Echinacoside and acteoside served as dual templates, 4-vinylpyridine (4-VP) was used as a functional monomer , the cross-linker was ethylene glycol dimethacrylate (EGDMA), and the selected initiator was azobisisobutyronitrile (AIBN). The prepared Fe 3 O 4 @MCM-41-MIPs were applied to the selective separation and enrichment of echinacoside and acteoside from Cistanche deserticola Y. C. Ma ( C. deserticola ). The resulting Fe 3 O 4 @MCM-41-MIPs were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), laser particle size scanning and vibration sample magnetometry (VSM). The results of the isotherm adsorption experiment showed that the maximum adsorption capacities of Fe 3 O 4 @MCM-41-MIPs for echinacoside and acteoside were 6.02 and 1.63 mg·g -1 , respectively, at 298 K. The pseudo-second-order reaction mechanism showed that the equilibrium adsorption capacities of Fe 3 O 4 @MCM-41-MIPs for echinacoside and acteoside were 4.81 and 0.93 mg·g -1 , respectively. The characterization of Fe 3 O 4 @MCM-41-MIPs showed that the imprinted layer with dual-template molecules was wrapped on the outside of the carrier, and the obtained Fe 3 O 4 @MCM-41-MIPs showed good saturation magnetization , desirable capacity for echinacoside and acteoside adsorption, and satisfactory reuse (echinacoside and acteoside maintained relative adsorption rates of 97.5% and 95.8% after five cycles). After separation, the purities of echinacoside and acteoside were 33.50% and 27.00%, respectively. In addition, in the selective adsorption experiment, the synthesized Fe 3 O 4 @MCM-41-MIPs showed better selectivity for echinacoside and acteoside than their structural analogs. Therefore, this method proved to be sustainable, rapid and highly efficient for the separation and enrichment of echinacoside and acteoside.
研究领域:
中药化学
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分离科学
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产品关联
DOI
高影响因子
EXPERIMENTAL EYE RESEARCH
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2023
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IF 3.4
Jingfei Yang,Zhijuan Hua,Zhikun Zheng,Xuan Ma,Liang Zhu,Yan Li
Diabetes retinopathy (DR) is one of the most common microvascular complications of diabetes. Retinal pigment epithelial (RPE) cells exposed to a high glucose environment experience a series of functional damages, which is an important factor in promoting the progression of DR. Acteoside (ACT) has strong antioxidant and anti-apoptotic properties, but the mechanism of ACT in DR is not completely clear. Therefore, the purpose of the present study was to explore whether ACT inhibits the damage to RPE cells in a high glucose environment through antioxidative effects to alleviate the DR process. The DR in vitro cell model was constructed by treating RPE cells with high glucose, and the DR in vivo animal model was constructed by injecting streptozotocin (STZ) into the peritoneal cavity of mice to induce diabetes. The proliferation and apoptosis of RPE cells were detected by CCK-8 and flow cytometry assays, respectively. The expression changes in Nrf2, Keap1, NQO1 and HO-1 were evaluated by qRT‒PCR, Western blot and immunohistochemistry analyses. The MDA, SOD, GSH-Px and T-AOC contents were detected by kits. The changes in ROS and nuclear translocation of Nrf2 were observed by immunofluorescence assays. HE staining was used to measure the thickness of the outer nuclear layer (ONL) of the retina, and TUNEL staining was used to detect the number of apoptotic cells in the retinas of mice. In the present study, ACT effectively ameliorated outer retina damage in diabetic mice. In high glucose (HG)-induced RPE cells, ACT treatment had the following effects: improved proliferation, decreased apoptosis, inhibited Keap1 expression, promoted the nuclear translocation and expression of Nrf2, upregulated NQO1 and HO-1 (the target genes of Nrf2) expression, decreased ROS concentration, and increased the levels of the SOD, GSH-Px and T-AOC antioxidant indicators. However, knockdown of Nrf2 reversed the above phenomena, which indicated that the protective function of ACT in HG-induced RPE cells are closely related to Nrf2. In summary, the present study demonstrated that HG-induced oxidative stress injury is inhibited by ACT in RPE cells and the outer retina through the Keap1/Nrf2/ARE pathway.
研究领域:
药理学
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细胞生物学
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分子生物学
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糖尿病研究
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眼科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
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2024
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IF 3.8
Mujin Fang,Chen Chen,Yingying Fan,Qiong Zhang,Kui Li,Guoqing Yang,Renpan Deng,Xueqin Li
Molecularly imprinted nanocomposite membranes (MINMs) have shown great superiority in selective separation of acteoside (ACT) from phenylethanoid glycosides in Cistanche tubulosa . Herein, ACT-based MINMs (A-MINMs) with coral reef-like imprinted structure were proposed and developed for specifically separating ACT molecules. The nanospheres with hydrophilic multicores (NHMs) were introduced into polyvinylidene fluoride (PVDF) powders to obtain NHMs@PVDF membranes by a phase inversion method. Subsequently, the designed coral reef-like imprinted structure was constructed on NHMs@PVDF membrane-based surface. The A-MINMs with coral reef-like imprinted structure had dendritic and porous properties, which helped to form abundant ACT-imprinted cavities and sites of A-MINMs. In addition, the hydrophilic multicores and void space of NHMs together formed the affinity capture cages for hydrophilic ACT, enhancing rebinding capacity and permselectivity of A-MINMs. Therefore, A-MINMs with coral reef-like imprinted structure exhibited the high rebinding capacity (110.95 mg/g), rebinding selectivity of 5.15 and outstanding permselectivity of 10.04 toward ACT molecules. Moreover, the designed strategy of A-MHIMs with coral reef-like imprinted structure provides a new feasible method for permselectivity separation of other bioactive components.
研究领域:
材料科学
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生物化学
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制药工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2020
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IF 3.69
Yi Chen,Ying-Qi Li,Jia-Yi Fang,Ping Li,Fei Li
Ethnopharmacological relevance Cistanche tubulosa is a precious traditional Chinese medicine that has been widely used in the treatment of osteoporosis and Alzheimer's disease . Echinacoside and acteoside are the main active constituents in Cistanche tubulosa that have the pharmacological activities with research value. It has been reported that echinacoside and acteoside could improve the learning and memory ability, promote the proliferation and differentiation of osteoblast. Aim of study Echinacoside and acteoside from Cistanche tubulosa have shown significant activities of anti-osteoporosis and anti-Alzheimer's disease, while these effects have not been studied concurrently in a rat model. The aim of this study was to establish and verify the model of osteoporosis combined with Alzheimer's disease in rat, and to investigate the double effects of echinacoside and acteoside on this concurrent model. Materials and methods Three model groups of ovariectomy (OVX), sham surgery with D-galactose and AlCl 3 (D), ovariectomy with D-galactose and AlCl 3 (OVX + D) were set at the same time. The rats in drug treatment groups were ovariectomized. While conducting the intraperitoneal injection of D-galactose and intragastric administration of AlCl 3 in the rats of drug treatment groups, the rats were orally administered echinacoside (90 mg/kg/d), acteoside (90 mg/kg/d) and the positive control drugs of estradiol valerate (0.6 mg/kg/d), donepezil HCl (0.8 mg/kg/d), respectively. After the drug treatment of 8 weeks, Morris Water Maze (MWM) test for 6 days was firstly performed. The rats were then sacrificed to harvest the blood, uteri, femora, tibiae and brain tissues. The serum was used for biochemical tests. The uteri were used for histomorphometry. The right femora were used for Micro-CT and histomorphometry, respectively. The right tibiae were used for biomechanical test. The hippocampus collected on ice box was used for biochemical tests. The brain collected by perfusion was used for histomorphometry. Results Compared with Sham group, OVX + D group could significantly reduce the learning and memory ability by causing oxidative damage, impairing neurons in hippocampus and affecting the hydrolysis and synthesis of acetylcholine . Meanwhile, the activities of BALP and TRAP in OVX + D group increased significantly ( P < 0.001) as compared to Sham group. In addition, compared with Sham group, the mean bone mineral density obviously decreased ( P < 0.05), the trabecular bone mass and microarchitecture were also destroyed significantly in OVX + D group. Furthermore, the maximum load and maximum stress significantly reduced ( P < 0.01) and the energy absorption also decreased greatly as compared to Sham group. After administrated with echinacoside and acteoside, the typical pathological features of osteoporosis and Alzheimer's disease were ameliorated. Conclusions The model of osteoporosis combined with Alzheimer's disease in rat was feasible and successfully established. Echinacoside and acteoside also showed some significant effects on this concurrent model, and they could be potential candidates from Cistanche tubulosa with double effects for further study.
研究领域:
药理学
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神经科学
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老年医学
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DOI
PubMed
高影响因子
Frontiers in Cell and Developmental Biology
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2021
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IF 6.684
Li Ying-Qi,Chen Yi,Jiang Si-Qi,Shi Yuan-Yuan,Jiang Xiao-Li,Wu Shan-Shan,Zhou Ping,Wang Hui-Ying,Li Ping,Li Fei
Alzheimer’s disease (AD) is the most frequent type of dementia. Acteoside (ACT) is a compound isolated from Cistanche tubulosa, which possesses excellent neuroprotective properties. However, the underlying mechanism of ACT in regulating microglia polarization remains ill-defined. Therefore, a computational network model was established to identify the driving targets of ACT and predict its mechanism by integrating multiple available databases. The AlCl3-induced AD model in zebrafish larvae was successfully constituted to demonstrate the therapeutic efficacy of ACT. Subsequently, LPS-induced BV-2 cells uncovered the positive role of ACT in M1/M2 polarization. The NF-κB and AMPK pathways were further confirmed by transcriptomic analysis, metabolomics analysis, molecular biology techniques, and molecular docking. The research provided an infusive mechanism of ACT and revealed the connection between metabolism and microglia polarization from the perspective of mitochondrial function. More importantly, it provided a systematic and comprehensive approach for the discovery of drug targets, including the changes in genes, metabolites, and proteins.
研究领域:
神经科学
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药理学
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分子生物学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）