AMB Express
·
2018
·Vol. 8
·期 1
·1-13
·
IF 1.719
Liang Jibei,Cheng Tao,Huang Yi,Liu Jianhua
Enhanced bioremediation is a favorable approach for petroleum pollutant cleanup, which depends on the growth of oil-eating microorganisms. In this study, we show that, by using the modified T-RFLP (mT-RFLP) methodology, one of the four major microbial populations derived from oil sludge has failed to propagate in MS medium supplemented with 2% yeast extract (YE). rDNA sequence-based analysis indicated that the four populations were Donghicola sp. CT5, Bacillus sp. CT6, Alcaligenes sp. CT10, and Pseudomonas sp. ZS1. Four purified strains grow well individually in MS medium supplemented with 2% YE, suggesting that ZS1 growth is antagonized by other strains. Co-growth analysis using mT-RFLP methodology and plate inhibitory assay indicated that ZS1 exhibited antagonistic effect against CT5 and CT6. On the other hand, co-growth analysis and plate inhibition assay showed that CT10 antagonized against ZS1. To investigate the potential compounds responsible for the antagonism, supernatant of CT10 culture was subjected to GC–MS analysis. Analysis indicated that CT10 produced a number of antimicrobial compounds including cyclodipeptide c-(L-Pro-L-Phe), which was known to inhibit the growth of Pseudomonas sp. Growth test using the purified c-(L-Pro-L-Phe) from CT10 confirmed its inhibitory activity. We further showed that, using both gravimetric and GC analysis, CT10 antagonism against the oil-eating ZS1 led to the diminishing of crude oil degradation. Together, our results indicate that bioremediation can be affected by environmental antagonists.
研究领域:
微生物学
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环境科学
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DOI
PubMed
高影响因子
Water
·
2023
·Vol. 15
·期 23
·4084
·
IF 3.4
Zhi Zeng,Jiahui Xiao,Manzhi Li,Jiahui Wu,Taiping Zhang
The effect and mechanism of phenol removal by immobilized microorganisms in Fe3O4nanomaterial-modified pharmaceutical residue biochar was investigated to develop efficient biochar immobilizing microbial technology. Plant residue biochar (Y3, Y5, and Y7) was prepared fromAndrographis paniculataplant residues as the raw material at 300 °C, 500 °C, and 700 °C, respectively. Y7 was modified with Fe3O4nanomaterial (Fe-Y7). These four kinds of biochars were used as carriers to adsorb immobilizedAlcaligenes faecalisstrain JH1, JY3, JY5, JY7, and Fe-Y7 to investigate the mechanism of phenol removal, and eight cycles were performed to analyze their immobilization performance. Compared with suspended bacteria, biochar-immobilized bacteria could improve their tolerance in different environments. At temperatures of 25 °C to 40 °C, pH = 5~9, initial phenol concentration of 300–500 mg/L, and salinity of 3%, the bacteria could still grow and maintain strong activity within 48 h. The water-extractable organic carbon of biochar was also tested for the degradation of phenol by bacteria, which was found to have different stimulating effects on bacteria. In the batch experiments, as the number of cycles increased, the bacteria grew and adhered rapidly to the biochar, eventually forming a thick and sticky biofilm. After the sixth cycle, all the biochar-immobilized bacteria could remove 300 mg/L phenol solution within 12 h. The removal rate of phenol by JFe-Y7 was relatively fast in the eighth cycle. The results indicated that biochar-immobilized bacteria have good durability, stability, and reproducibility and that Fe3O4nanoparticle modification could improve the removal of phenol by increasing the phenol adsorption amount, the adsorption capacity of bacteria, and the enzymatic activity of bacteria.Keywords:biochar;immobilization;bacteria;phenol;mechanism
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高影响因子
BIORESOURCE TECHNOLOGY
·
2024
·
IF 9.7
Xinyi Li,Tianbo Jia,Haiguang Zhu,Luhan Cai,Yubiao Lu,Jianxin Wang,Hengcong Tao,Peng Li
The CO 2 fixation mechanism by Alcaligenes faecalis ZS-1 in a biocathode microbial fuel cell (MFC) was investigated. The closed-circuit MFC (CM) exhibited a significantly higher CO 2 fixation rate (10.7%) compared to the open-circuit MFC (OC) (2.0%), indicating that bioelectricity enhances CO 2 capture efficiency. During the inward extracellular electron transfer (EET) process, riboflavin concentration increased in the supernatant while cytochrome levels decreased. Genome sequencing revealed diverse metabolic pathways for CO 2 fixation in strain ZS-1, with potential dominance of rTCA and C4 pathways under electrotrophic conditions as evidenced by significant upregulation of the ppc gene. Differential metabolite analysis using LC-MS demonstrated that CM promoted upregulation of various lipid metabolites. These findings collectively highlight that ZS-1 simultaneously generated electricity and fixed CO 2 and that the ppc associated with bioelectricity played a critical role in CO 2 capture. In conclusion, bioelectricity resulted in a significant enhancement in the efficiency of CO 2 fixation and lipid production.
研究领域:
环境科学
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微生物学
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生物化学
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基因组学
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DOI
PubMed
高影响因子
Journal of Water Process Engineering
·
2023
·
IF 7
Guangxu Bo,Liangcheng Jiao,Huanhuan Li,Guowei Zhao,Zitang Xu,Xiaoxiao Liu,Pengbo Wang,Xiaoke Tian,Jinyong Yan,Xueqing Wang,Yunjun Yan
In this study, the removal efficiencies of bio-based rigid polyurethane foams (“RPUFs” group), Alcaligenes faecalis subsp. faecalis NBRC 13111 T (“strain” group), and the combined use of RPUFs and A. faecalis subsp. faecalis NBRC 13111 T (“RPUFs + strain” group) were investigated. The concentration of NaCl, number of RPUFs, initial pH and initial phenol concentration were sequentially examined. The results showed the adsorption of phenol in “RPUFs” group and “RPUFs + strain” group was improved with the increased number of RPUFs, and the highest adsorption was about 200 mg/L when 40 pieces of RPUFs were used. Under all conditions, the phenol removal efficiency of “RPUFs + strain” group was higher than that of the “strain” group. With the increased concentration of NaCl, the complete removal time of phenol in “strain” group and “RPUFs + strain” group was correspondingly prolonged. In neutral and weakly alkaline conditions, the phenol removal efficiency of “strain” group and “RPUFs + strain” group was much improved, and the phenol-removed time of 500 mg/L of “RPUFs + strain” group was only 30 h at the pH of 7. With the increase of initial phenol concentration, the complete removal time in “strain” group and “RPUFs + strain” group was delayed, especially at the high concentration of 1100 mg/L, the “strain” group had nearly no ability to remove phenol, while the “RPUFs + strain” group could still completely removed phenol at 78 h. The phenol removal mechanism of the “RPUFs + strain” group was attributed to “physical adsorption - biodegradation”.
研究领域:
环境科学
·
微生物学
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DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2021
·
IF 6.498
Xingyao Ye,Xiaoxian Zheng,Dongqing Zhang,Xiaojun Niu,Yiming Fan,Wangde Deng,Zhang Lin,Huijian Hu,Shaoqi Zhou
Cadmium-contaminated wastewater has attracted increasing concerns due to its non-biodegradable properties and high toxicity. To explore eco-friendly and economically feasible strategies, the screened Alcaligenes faecalis K2 were employed for the biomineralization and recovery of Cd 2+ from wastewater while producing considerable secretory organo-biominerals (SOBs) as bioadsorbents. At 75 mg/L Cd 2+ exposure, 85.5% of Cd 2+ was removed by K2, 43.0% of which was fixed in the granular SOBs. SOBs were convenient for separating from the solution. The adsorption capacity of granular sorbent made from SOBs was verified to be greater than 77.1 mg/g. Practically, 89.5% of 75 mg/L of Cd 2+ could be stably removed while ereK2 continuously generated SOBs in a moving-bed biofilm reactor (MBBR). To sum up, the production of bioadsorbents can be achieved by K2, while removing Cd with live microorganisms , which was conducive to making full use of materials and improving Cd removal efficiency.
研究领域:
环境科学
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生物技术
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废水处理
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微生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 32
·48638-48647
·
IF 5.19
Li Qian,Tang Zeqin,Zhang Jiahui,Hu Jingtao,Chen Jianmeng,Chen Dongzhi
Simultaneous biodegradation of malodorous 1-propanethiol (PT) and dimethyl sulfide (DMS) by Pseudomonas putida S-1 and Alcaligenes sp. SY1 was investigated and the interactions implicated were explored. Results showed that PT was completely degraded in 33 h, while a lag of 10 h was observed for DMS degradation alone, and the lag was even extended to 81 h in the binary mixture. Mechanism analysis found that the lag was mainly attributed to the exposure of DMS degrader ( Alcaligenes sp. SY1), rather than PT metabolites and PT degrader. The exposure time and PT concentration also influenced the lag duration much. Citric acid could effectively reduce the lag. Pseudo-first-order model was proved suitable for the description of PT degradation, revealing that PT degradation could be enhanced in presence of DMS with a concentration of < 50 mg L −1 . A modified Gompertz model, incorporated the lag phase, was developed for the description of DMS degradation in the mixture, revealing that DMS degradation depended on the initial PT concentration, and when the lag was not considered, PT with low-concentration could promote DMS biodegradation, while a higher concentration (> 20 mg L −1 ) cast negative effect.
研究领域:
环境生物学
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微生物学
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DOI
PubMed
高影响因子
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