查找使用阿拉丁产品的科研文献

Impact of chronic exposure to field-level glyphosate on the gut microbiota of Apis cerana and Apis mellifera

Changsheng Ma,Sihao Chen,Xiaoyu Shi,Lianyang Bai,Hongmei Li-Byarlay,Zuren Li

Glyphosate (GLY) stands as one of the most extensively used pesticides globally. Although several studies have investigated the effects of sublethal doses of GLY on the gut microbiota of bees, limited information is available on how residual concentrations of GLY in bee food affect Apis cerana (Asian honey bees) and Apis mellifera (European honey bees) at different time points. To explore these effects, we employed 16S rRNA Illumina sequencing technology to evaluate the consequences of chronic GLY exposure (0.7 mg/kg, GLY residual in beebread) on the gut microbiome of A. cerana and A. mellifera on days 3 and 12 of the exposure period. Even though our findings revealed no significant difference in the microbiota abundance between control bees and GLY-treated bees on the 3rd day, GLY-treated bees exhibited 55.56 % abundance of the genus Snodgrassella (a core gut bacterium) and 4.54 % of the genus Commensalibacter (a non-core gut bacterium) compared to control bees in A. cerana and A. mellifera , respectively. In addition, our results showed that a noticeable trend indicated that the gut of A. mellifera was more susceptible to GLY than that of A. cerana . Considering the vital role of gut microbiota in the health of honey bee colonies, our results suggest that 0.7 mg/kg of GLY are likely to have adverse effects on the health of honey bees.

Composition of Strawberry Flower Volatiles and Their Effects on Behavior of Strawberry Pollinators, Bombus terrestris and Apis mellifera

Jinjia Liu,Min Chen,Weihua Ma,Lifang Zheng,Bing Zhang,Huiting Zhao,Yusuo Jiang

Strawberries are popular fruits around the world, and their yield and fruit quality rely on pollination by honey bees and bumblebee colonies. Both bee species have their own advantages in strawberry pollination. This study investigates the characteristic of strawberry (Fragaria×ananassa‘Red Face’) flower volatiles and their effects on bee pollinators by (1) detecting the volatile compounds of strawberry flowers by polydimethylsiloxane (PDMS) combined gas chromatography-mass spectrometry (GC-MS), (2) determining whetherBombus terrestrisorApis melliferashowed antennae responses to certain compounds of strawberry flower volatiles by an electroantennography test (EAG), and (3) testing whether these compounds could elicit a corresponding behavioral response in bees. The results showed that (1) there were 38 chemical compounds in ‘Red Face’ volatiles with 7 types, most of which were known to be generally emitted by flowers but also have some compounds that have not been reported in strawberry flowers; (2)B. terrestrisandA. melliferahad strong EAG responses to several compounds, respectively, especially to ethyl benzoate, (Z)-3-hexenyl propionate, (Z)-3-hexenyl acetate, benzeneacetaldehyde and melonal; and (3) both bee species showed significant avoidance behaviors to four tested compounds, especially theB. terrestris. Flower volatiles of strawberry ‘Red face’ were different from other strawberry varieties that have been reported; some of these electrophysiologically active compounds could cause antennal potential responses in bees, as well as behavioral responses. Our study supports the idea that strawberry flower volatiles are one of the factors influencing bee foraging decisions and provides a reference for formulating more reasonable bee pollination to improve strawberry fruit quality.Keywords:strawberry flower volatiles;Bombus terrestris;Apis mellifera;EAG response;behavior response

Corrigendum: Resisting majesty: Apis cerana, has lower antennal sensitivity and decreased attraction to queen mandibular pheromone than Apis mellifera

Shihao Dong,Ping Wen,Qi Zhang,Xinyu Li,Ken Tan,James Nieh

Evaluating the combined toxicity of broflanilide and myclobutanil on honeybees (Apis mellifera L.): Molecular mechanisms and protective effects of curcumin

Rongyi Ye,Ran Yi,Yihan Wang,Nan Huang,Yanhua Wang,Chen Chen

Pesticide toxicity to honeybees has become a pressing ecological issue, yet the effects of pesticide co-exposure are still not fully understood. This research investigates the toxicological implications of concurrent exposure to broflanilide (BFL), a novel diamide insecticide, and myclobutanil (MYC), a commonly used triazole fungicide, on honeybees ( Apis mellifera L . ), while exploring potential preventive strategies. Acute toxicity tests revealed a significantly lower 96-hour lethal concentration 50 (LC 50 ) for BFL (0.34 mg a.i. L −1 ) compared to MYC (82.3 mg a.i. L −1 ), and their co-exposure resulted in pronounced synergistic toxicity. Worker bees were exposed to environmentally relevant doses of BFL and MYC for 7 days, and midgut toxicity was assessed. The co-exposure caused severe midgut damage, including G-layer deterioration, loss of columnar epithelium integrity, and downregulation of the tight junction protein Z O-2. Additionally, oxidative stress-related genes ( Sod1 , Catalase , SelK , GstD1 ) were upregulated, accompanied by higher MDA levels and increased CAT and SOD activities. Furthermore, a greater number of TUNEL-positive cells were detected, along with elevated expression of apoptosis-related genes ( Caspase-3-like , Caspase-8-like , Caspase-9-like ) and higher caspase enzyme activities. Curcumin (Cur) was tested for its protective effects, and it significantly alleviated midgut damage, oxidative stress, and apoptosis. This study reveals the synergistic ecotoxicological effects of pesticide combinations and suggests Cur as a potential prevention strategy for mitigating their harmful impact on honeybees.

Stress responses in the honeybee (Apis mellifera L.) midgut triggered by PFOA and PFHxS at environmental residue concentrations detected in bee products

Tao Cang,Lu Lv,Yue Yin,Dou Wang,Kan Shao,Zhenlan Xu,Xinquan Wang,Yanhua Wang

Perfluorooctanoic acid (PFOA) and perfluorohexane sulfonate (PFHxS), as persistent global contaminants, pose substantial threats to pollinators like honeybees ( Apis mellifera L.), yet their sub-lethal effects remain insufficiently explored. Here, we exposed bees to environmentally relevant concentrations (0.5–50 μg/L) of PFOA and PFHxS for 10 days, assessing midgut toxicity through pathology, metabolomics, and gene expression, and examined curcumin (Cur) as a potential mitigator. Although acute toxicity assays indicated relatively high LC₅₀ values (266–331 mg/L), sub-lethal exposures induced pronounced midgut injury, characterized by decreased epithelial cell density and thinning of the protective gel layer. Disruption of glycerophospholipid metabolism was detected, impairing membrane integrity. Additionally, PFOA and PFHxS downregulated barrier integrity genes ( ZO-2 and Mucin-1 ), while upregulating inflammatory ( Eiger and Relish ) and apoptotic indicators ( Caspase-3 , Caspase-8 , and Caspase-9 ). For several sub-lethal endpoints, PFHxS triggered more pronounced effects than PFOA. Notably, curcumin treatment restored midgut integrity and reversed many of the associated molecular disruptions. These results demonstrated that environmentally relevant concentrations of PFAS could substantially compromise honeybee gut health, offering critical insights for ecological risk assessments aimed at safeguarding these essential pollinators.

Odor-Binding Protein 2 in Apis mellifera ligustica Plays Important Roles in the Response to Floral Volatiles Stimuli from Melon and Tomato Flowers

Jiangchao Zhang,Weihua Ma,Yue Zhang,Surong Lu,Chaoying Zhang,Huiting Zhao,Yusuo Jiang

Honeybee olfaction can influence foraging behavior and affect crop pollination. Odor-binding proteins play a vital role in honeybee olfactory perception. A previous study based on the antennal transcriptome ofApis mellifera ligusticain melon and tomato greenhouses revealed thatAmelOBP2is highly expressed. Therefore, we aimed to further investigate the olfactory recognition mechanism of honeybees by detecting the expression levels and binding ability of AmelOBP2 to floral volatiles of melon and tomato flowers. The results show thatAmelOBP2mRNA was highly expressed in the antennae of honeybees, and its protein expression was highest in the antennae at 20 days of age and was higher in the melon greenhouse. The binding ability of AmelOBP2 to floral volatiles of melon was stronger than that of tomato. AmelOBP2 had a stronger binding ability with aldehydes in melon floral volatiles and with terpenes and benzenes in tomato floral volatiles. After feeding with siRNA, the electroantennogram response of honeybees to E-2-hexenal, E-2-octenal, and 1-nonanal decreased markedly, confirming the role of AmelOBP2 in the recognition of melon and tomato floral volatiles. These results elucidate the molecular mechanisms underlying honeybee flower-visiting behavior and provide a theoretical reference for regulating the behavior of honeybees using plant volatiles.

Optimized nanopesticide delivery of thiamethoxam to cowpeas (Vigna unguiculata) controls thrips (Megalurothrips usitatus) and reduces toxicity to non-target worker bees (Apis mellifera)

Wenjie Deng,Yanheng Zhang,Liangheng He,Li Xu,Xulang Ye,Hanhong Xu,Li Zhu,Jinliang Jia

Thrips [ Megalurothrips usitatus (Bagnall)] (Thysanoptera: Thripidae) is a pest that poses a serious challenge to global crop production and food supply, especially to the cowpea industry. Nano-delivery systems have broad application prospects in the prevention and control of pests in agriculture. Herein, three types of amino acid (AA) modified polysuccinimide nano-delivery carriers (PSI-GABA, PSI-ASP and PSI-GLU) were constructed with a diameter of approximately 150 nm to load thiamethoxam (THX), which enhanced THX effective distribution and use with cowpea plants. Significantly, the PSI-GLU nanocarrier effectively delivered THX to cowpea plant tissues following 6 h of soil application. Compared with commercial THX suspension (SC), the THX content in the leaves of cowpea plants was increased by 2.3 times. Confocal laser scanning microscopy revealed that the FITC-labeled PSI-GLU nanocarrier reached the leaves through the vascular system after being absorbed by the roots of cowpea plants. The PSI-GLU nanocarrier decreased the LC 50 of THX from 11.45 to 7.79 mg/L and significantly enhanced the insecticidal effect. The PSI-GLU nanocarrier also improved the safety of THX to worker bees at 48 h, and moreover showed a growth-promoting effect on cowpea seedlings. These results demonstrated that the PSI-GLU nano-delivery carrier has promising uses on improving the effective utilization of THX for the sustainable control of thrips and reducing the risk to non-target pollutions.

Use of an innovative T-tube maze assay and the proboscis extension response assay to assess sublethal effects of GM products and pesticides on learning capacity of the honey bee Apis mellifera L.

Han Peng,Niu Chang-Ying,Lei Chao-Liang,Cui Jin-Jie,Desneux Nicolas

Transgenic Cry1Ac+CpTI cotton (CCRI41) is a promising cotton cultivar throughout China but side effects and especially sublethal effects of this transgenic cultivar on beneficial insects remain poorly studied. More specifically potential sublethal effects on behavioural traits of the honey bee Apis mellifera L. have not been formally assessed despite the importance of honey bees for pollination. The goal of our study was to assess potential effects of CCRI41 cotton pollen on visual and olfactory learning by honey bees. After a 7-day oral chronic exposure to honey mixed with either CCRI41 pollen, imidacloprid-treated conventional pollen (used as positive sublethal control) or conventional pollen (control), learning performance was evaluated by the classical proboscis extension reflex (PER) procedure as well as a T-tube maze test. The latter assay was designed as a new device to assess potential side effects of pesticides on visual associative learning of honey bees. These two procedures were complementary because the former focused on olfactory learning while the latter was involved in visual learning based on visual orientation ability. Oral exposure to CCRI41 pollen did not affect learning capacities of honey bees in both the T-tube maze and PER tests. However, exposure to imidacloprid resulted in reduced visual learning capacities in T-tube maze evaluation and decreased olfactory learning performances measured with PER. The implications of these results are discussed in terms of risks of transgenic CCRI41 cotton crops for honey bees.