Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2014
·Vol. 298
·期 3
·501-512
·
IF 1.53
Shuaishuai Huang,Yu Ren,Ping Wang,Yanyuan Li,Xue Wang,Haihui Zhuang,Rong Fang,Yuduo Wang,Ningsheng Liu,Michael Hehir,Jeff X. Zhou
Our previous studies illustrated that a steady increase of intracellular calcium concentration ([Ca 2+ ]i) was important for maintaining microtubules (MTs) rearrangement in apoptotic cells. However, little is known about the effect of calcium sensing receptor (CaSR)-mediated increase in [Ca 2+ ]i on cytoskeleton gene expression. We examined the impact of taxol or CaSR agonist/antagonist on the regulation of [Ca 2+ ]i concentration, cytoskeleton arrangement, phosphorylated CREB and cytoskeleton gene expressions in HeLa cells with dominant negative plasmid of CREB (PM). This study demonstrated that Gdcl3 (a specific CaSR agonist) evoked a rapid increase of [Ca 2+ ]i, formed a rigid bundle of MTs which surrounded the nucleus and decreased the cytoskeleton gene expressions in HeLa cells. These effects were rescued by addition of NPS2390 (a specific CaSR antagonist). Moreover, CaSR activity affected cytoskeleton gene expression through transcription factor CREB. Histoscores of pCREB immunoreactivity in tissues of cervical adenocarcinoma, renal clear cell carcinoma, and diffuse large B-cell lymphoma were markedly increased compared with non malignant tissue. These data demonstrate, for the first time, that CaSR-mediated increase in [Ca 2+ ]i probably modulate cytoskeleton organization and gene expression via transcription factor CREB. Anat Rec, 298:501–512, 2015. © 2014 Wiley Periodicals, Inc.
研究领域:
分子生物学
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细胞生物学
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肿瘤学
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DOI
PubMed
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2018
·Vol. 301
·期 6
·1115-1125
·
IF 1.373
Hui Wang,Xiaoguang Dong,Zengxun Liu,Shaowei Zhu,Haili Liu,Wenchuang Fan,Yanlai Hu,Tao Hu,Yonghui Yu,Yizhao Li,Tianwei Liu,Chengjia Xie,Qing Gao,Guibao Li,Jing Zhang,Zhaoxi Ding,Jinhao Sun
Rotenone is a common pesticide and has been reported as one of the risk factors for Parkinson disease. Rotenone can cause neuronal death or apoptosis through inducing oxidative injury and inhibiting mitochondrial function. As a natural polyphenolic compound, resveratrol possesses the antioxidant capacity and neuroprotective effect. However, the mechanism underlying the neuroprotective effect of resveratrol against rotenone-induced neurotoxicity remains elusive. Here, we treated PC12 cells with rotenone to induce neurotoxicity, and the neurotoxic cells were subjected to resveratrol treatment. The CCK8 and LDH activity assays demonstrated that resveratrol could suppress neurotoxicity induced by rotenone ( P < 0.01). The DCFH-DA assay indicated that resveratrol reduced the production of reactive oxygen species (ROS). JC-1 and Hoechst 33342/PI staining revealed that resveratrol attenuated mitochondrial dysfunction and cell apoptosis. Moreover, resveratrol reversed rotenone-induced decrease in SIRT1 expression and Akt1 phosphorylation ( P < 0.05). Furthermore, when the SIRT1 and Akt1 activity was inhibited by niacinamide and LY294002, respectively, the neuroprotective effect of resveratrol was remarkably attenuated, which implied that SIRT1 and Akt1 could mediate this process and may be potential molecular targets for intervening rotenone-induced neurotoxicity. In summary, our study demonstrated that resveratrol reduced rotenone-induced oxidative damage, which was partly mediated through activation of the SIRT1/Akt1 signaling pathway. Our study launched a promising avenue for the potential application of resveratrol as a neuroprotective therapeutic agent in Parkinson disease. Anat Rec, 301:1115–1125, 2018. © 2018 Wiley Periodicals, Inc.
研究领域:
神经科学
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药理学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2018
·Vol. 301
·期 7
·1179-1188
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IF 1.373
Wei Cao,Junqiang Zhang,Gaoyuan Wang,Jinsen Lu,Taorong Wang,Xiaoyu Chen
In rheumatoid arthritis patients, the fibroblast-like synovial cells (FLS) growth is not controlled normally, but is similar to the tumor cells proliferation in histology. Our previous studies have shown that resveratrol inhibits the proliferation of FLS and promotes FLS apoptosis. However, the molecular mechanisms involved in resveratrol-induced FLS apoptosis have not been determined yet. Here, we showed that the FLS cell viability (following pretreatment with 5 µM H 2 O 2 for 24 hr) exhibited better proliferation performance than at other concentrations via the CCK-8 assay. The cell apoptotic rate increased with the increasing concentration of resveratrol (0, 40, 80, 160, 320 μM), as detected by TdT-mediated dUTP nick-end labeling (TUNEL) staining and western blotting. Furthermore, the expression level of autophagy-related proteins (LC3A/B, ATG-5) decreased with the increased concentration of resveratrol, as determined by immunofluorescence and western blot analysis. We also showed that resveratrol induced FLS mitochondrial morphology change. Moreover, mitochondrial function detection showed that the mitochondrial membrane potential was lost with the increased concentration of resveratrol as examined by the JC-1 assay. The production of ATP in cells was positively and negatively correlated with the resveratrol concentration. Simultaneously, the intracellular calcium release and calcium influx decreased gradually with the increase in resveratrol concentration. Therefore, we proposed that resveratrol can reduce the level of autophagy in FLS. The decrease in the autophagy level can lead to the accumulation of reactive oxygen species, which may result in mitochondrial dysfunction and promotion of FLS apoptosis. Anat Rec, 301:1179–1188, 2018. © 2018 Wiley Periodicals, Inc.
研究领域:
药理学
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细胞生物学
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免疫学
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风湿病学
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产品关联
DOI
PubMed
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
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2016
·Vol. 299
·期 4
·450-460
·
IF 1.507
Fuyin Hou,Min Xiao,Jian Li,Devin W. Cook,Weidong Zeng,Caiqiao Zhang,Yuling Mi
Cadmium (Cd) is an environmental endocrine disruptor that has toxic effects on the female reproductive system. Here the ameliorative effect of grape seed proanthocyanidin extract (GSPE) on Cd-induced meiosis inhibition during oogenesis was explored. As compared with controls, chicken embryos exposed to Cd (3 µg/egg) displayed a changed oocyte morphology, decreased number of meiotic germ cells, and decreased expression of the meiotic marker protein γH2AX. Real time RT-PCR also revealed a significant down-regulation in the mRNA expressions of various meiosis-specific markers ( Stra8 , Spo11 , Scp3 , and Dmc1 ) together with those of Raldh2 , a retinoic acid (RA) synthetase, and of the receptors ( RARα and RARβ ). In addition, exposure to Cd increased the production of H 2 O 2 and malondialdehyde in the ovaries and caused a corresponding reduction in glutathione and superoxide dismutase. Simultaneous supplementation of GSPE (150 µg/egg) markedly alleviated the aforementioned Cd-induced embryotoxic effects by upregulating meiosis-related proteins and gene expressions and restoring the antioxidative level. Collectively, the findings provided novel insights into the underlying mechanism of Cd-induced meiosis inhibition and indicated that GSPE might potentially ameliorate related reproductive disorders. Anat Rec, 299:450–460, 2016. © 2016 Wiley Periodicals, Inc.
研究领域:
毒理学
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生殖生物学
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分子生物学
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DOI
PubMed
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2023
·
IF 2
Guanghong Shen,Chengjian Lou,Qunfeng Li,Bingxin Zhao,Yuhuan Luo,Fei Wu,Dian Jiao,Marong Fang,Yu Geng
Inflammatory injury following ischemia-reperfusion (I/R) severely limits the efficacy of stroke treatment. Edaravone dexborneol (C.EDA) has been shown to reduce inflammation following a cerebral hemorrhage. However, the precise anti-inflammatory mechanism of C.EDA is unknown. In this study, we investigated whether C.EDA provides neuroprotection after I/R in rats, as well as the potential mechanisms involved. A middle cerebral artery occlusion/reperfusion (I/R) model was created using Sprague-Dawley rats. The blood flow of the central cerebral artery was monitored by a laser speckle imaging system. The neurological score was used to assess behavioral improvement. Cerebral infarction volume was measured by TTC staining. And the integrity of the blood–brain barrier was detected by Evan's blue staining. The expression of the nuclear factor kappa-B (NF-κB)/ the NOD-like receptor protein (NLRP3) inflammasome signal pathway and microglia polarization were detected by immunofluorescence and Western blotting. The cerebral blood flow ratio indicates that the cerebral I/R model was successfully established. After reperfusion for 72 h, the improvement of neurological scores, infarct volume reduction, and integrity of the blood–brain barrier was observed in I/R rats with C.EDA treatment. Meanwhile, the immunofluorescence result showed that the expression of iNOS, NLRP3, and NF-κB protein was decreased and the level of Arg1 was increased. Western blot analysis showed that the expression of NF-κB/NLRP3 signal pathway-related protein was decreased. In conclusion, this study indicates that C.EDA alleviates I/R injury by blocking the activation of the NLRP3 inflammasome and regulating the polarization of M1/M2 microglia via the NF-κB signal pathway.
研究领域:
神经科学
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药理学
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炎症
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神经保护
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
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2019
·
IF 7.467
Qiulin Li,Heng Liu,Long-Cheng Zhang,Hao Chen,Hongjiu Zhu,Yuanke Wu,Maowen Xu,Shu-Juan Bao
Fe-N and pyridinic-N generally are considered the active sites of Fe-N-C catalyst. In this featured work, small uniform Fe/N-rich polymer spheres are selected to prepare the Fe-N-C catalyst and study how the sintering atmosphere (NH 3 , Ar, and H 2 ) to engineer their products. The findings reveal that the sintering atmosphere greatly affected not only the composition , aggregate state, and morphology of Fe, but also the graphitization degree and pore structure of carbon. In addition, XPS results suggest that Fe-N-C-NH 3 possesses the highest Fe-N and pyridinic-N content. Combining its abundant pores, high graphitization, and large specific surface area, the catalyst obtained in NH 3 atmosphere delivers best ORR catalytic performance (E 0 = 0.97 V, E 1/2 = 0.85 V vs RHE), which is even comparable to commercial Pt/C (E 0 = 0.97 V, E 1/2 = 0.83V vs RHE). Moreover, the catalyst exhibits greater stability and methanol resistance than those of Pt/C.
研究领域:
材料科学
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电化学
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DOI
高影响因子
PROCEEDINGS OF THE COMBUSTION INSTITUTE
·
2022
·Vol. 39
·期 1
·315-324
·
IF 6.535
Qianjin Lin,Wanxiong Liao,Jia Cheng,Chun Zou,Bin Yang,Wenxiang Xia
Few studies on the low-temperature combustion behavior of MIPK, being a promising fuel additive , have been conducted. In this work, ignition delay times (IDTs) of MIPK were measured in the temperatures ranging between 780–910 K and pressures of 20 and 25 bar using a rapid compression machine (RCM). Oxy-fuel combustion combined with biofuel could remove CO 2 from the atmosphere. The IDTs of MIPK were measured in the temperatures ranging between 1125–1600 K under the O 2 /CO 2 atmosphere at the pressures of 1 and 10 bar using a shock tube . A low to high-temperature MIPK kinetic model (HUST-MIPK model) was proposed, in which the low-temperature sub-model consists of 19 low-temperature reaction classes and was constructed by analogy-based method, the high-temperature sub-model was adapted from the works of Cheng et al.. The predictions of HUST-MIPK model are in good agreement with the present low-temperature IDTs, high-temperature O 2 /CO 2 atmosphere IDTs, and the literature experimental data. The negative temperature coefficient (NTC) behavior was not observed in the temperature range from 790 to 910 K in the present RCM experiments, but was observed for methyl propyl ketone (MPK) and diethyl ketone (DEK) under similar conditions. The low-temperature chemistry of three pentanone isomers (MIPK, MPK, and DEK) was compared using the flux and sensitivity analysis. The comparison of the experimental high-temperature IDTs between O 2 /CO 2 and O 2 /Ar atmospheres indicates the IDTs of MIPK under O 2 /CO 2 atmosphere are longer than those under O 2 /Ar atmosphere at 1 bar, and the effects of CO 2 are almost independent of the pressure. The physical and chemical effects of CO 2 on the ignition were studied in detail.
研究领域:
燃烧化学
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燃料化学
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反应动力学
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能源工程
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大气环境影响
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DOI
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Zhijin Zhang,Wentao Zhang,Huan Wang,Haotian Chen,Hong Wang,Yang Yu,Danjing Shen,Man Pi,Yang Wu,Ming Luo,Yanyan He,Shiyu Mao,Jiang Geng,Wei Li,Guangchun Wang,Changcheng Guo,Daqiang Yin,Xudong Yao
Epidemiological studies indicate that prostate cancer (PCa) is the second prevalent malignant tumor affecting men globally. Environmental pollution such as cigarette smoke is one of the important risk factors for the development of prostate cancer. However, as one of the main carcinogens in cigarette smoke, the role of benzo[a]pyrene (BaP) in prostate cancer is still unclear. The current study aimed to investgate the impacts of BaP exposure on the progression of PCa toward malignancy and the regulation of the immune microenvironment. We verified that BaP exposure can promote the proliferation, migration, and apoptosis of prostate cancer cells through in vitro experiments. We constructed a subcutaneous xenograft tumor model of BaP exposure mouse and found that can promote the proliferation of tumors in vivo . Organoids-driven by PCa patients showed higher growth rate under BaP exposure. Flow cytometric analysis demonstrated a remarkable decrease in CD4 + T and CD8 + T cell infiltration levels. Moreover, we identified four genes ( Mdm2, Ar, Foxo1, Crebbp ) were strongly associated with BaP exposure by combining mouse tumor RNA-seq and CTD database. Additionally, a nomogram integrating clinicopathological features was constructed to assess the prognosis of prostate cancer patients under BaP exposure. This study systematically proved that BaP exposure promotes malignant progression of PCa and suppresses the immune microenvironment, in which Mdm2, Ar, Foxo1, Crebbp may play a crucial role in inhibiting apoptosis. These findings offer novel insights into the mechanisms via which BaP exposure contributes to PCa development.
研究领域:
肿瘤学
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免疫学
·
环境医学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·
IF 4.1
Wang Meng-Yun,Zhang Yue,Wang Dan,Yao Ming,Wang Yi-Xuan,Zhou Xing-Ping,Peng Hai-Yan,Xie Xiao-Lin
Holographic optical elements (HOEs) based on polymer composites have become a research hot spot in recent years for augmented reality (AR) due to the significant improvement of optical performance, dynamic range, ease of processing and high yield rate. Nevertheless, it remains a formidable challenge to obtain a large field of view (FOV) and brightness due to the limited refractive index modulation. Herein, we report an effective method to tackle the challenge by doping an epoxy liquid crystal termed E6M, which enables a large refractive index modulation of 0.050 @ 633 nm and low haze of 5.0% at a doping concentration of 5 wt%. This achievement may be ascribed to the improved molecular ordering of liquid crystals within the holographic polymer composites. The high refractive index modulation can endow transmission-type holographic polymer composites with a high diffraction efficiency of 96.2% at a small thickness of 5 µm, which would promise the design of thin and lightweight AR devices.
研究领域:
光学
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材料科学
·
光子学
·
增强现实
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产品关联
DOI
高影响因子
Advanced Science
·
2026
·
IF 14.1
Yong Luo,Tianlong Luo,Lingfeng Li,Qi Sun,Dongquan Li,Shanhe Huang,Zonglin Li,Weilong Lin,Yuan Ou,Tao Du,Shengmeng Peng,Kewei Xu,Bisheng Cheng,Hai Huang
Resistance to second-generation antiandrogens like enzalutamide (ENZ) in castration-resistant prostate cancer (CRPC) is a major clinical challenge, yet the role in the tumor microenvironment remains poorly understood. This study identifies a unique AR-positive tumor-associated macrophages (AR + TAMs) subpopulation, enriched in ENZ-resistant patients and correlated with poor prognosis, which acquires functional AR protein not through endogenous expression but via ANXA2-dependent phagocytosis of tumor cells. The internalized AR protein translocates to the macrophage nucleus, directly binds the IL-6 promoter to enhance its transcription and secretion. Macrophage-derived IL-6 subsequently activates the JAK2/STAT3 pathway in cancer cells, suppressing ENZ-induced apoptosis and conferring therapeutic resistance. Genetic or pharmacological blockade of IL-6 signaling restored ENZ sensitivity in vitro and in vivo, and combining an anti-IL-6 antibody with ENZ synergistically overcomes resistance in patient-derived xenograft and orthotopic models. These findings reveal a novel phagocytosis-mediated, paracrine mechanism of ENZ resistance orchestrated by AR + TAMs, challenging the tumor-centric view of therapy failure and providing a strong rationale for co-targeting the IL-6 pathway to improve outcomes of AR-directed therapy in CRPC.
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DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Kelin Hu,Feipeng Wang,Hongcheng Liu,Yanqiong Li,Wen Zeng
Pd-doped SnO 2 nanofibres were prepared by electrospinning and magnetron sputtering . It was found that the efficiency of gas sensing properties in these fibres depends primarily on the density of oxygen vacancies , which can be regulated by plasma treatment. We demonstrated that moderate plasma treatment can increase the oxygen vacancy as well as improve the gas sensing performance of SnO 2. However, excessive plasma treatment led to the production of other substances that deteriorated the gas sensing performance. Furthermore, the best results for hydrogen gas sensing were obtained when the nanofibres were subjected to plasma treatment for 1 min. The fabricated material demonstrated a response of more than 53 for 500 ppm hydrogen at the optimum temperature (130 °C), which is a significant improvement as compared to sensors without plasma treatment. Evidently, plasma treatment is an effective method to further enhance the sensing properties of the fabricated nanofibres.
研究领域:
材料科学
·
传感器技术
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产品关联
DOI
高影响因子
Device
·
2023
·
IF 0
Chang Gu,Yan Yan,Jiale He,Donglin Pu,Linsen Chen,Yu-Mo Zhang,Sean Xiao-An Zhang
Transparent non-emissive displays are ideal for augmented reality (AR) applications due to their ability to modulate ambient light as colored and transparent pixels. Electrochromic (EC) displays with transparent “ON” and “OFF” states can help enable such a display. However, unwanted image diffusion and signal crosstalk due to the imbalance between performance and the preparation process limit their practical application. In this work, we present a transparent and energy-efficient EC display prototype (4.2 inch, 16 × 16 pixels). We minimize the signal crosstalk problem by exploiting the confinement effect between EC pixels using the in situ photolithography of EC materials with the application of a photolithographic pixel definition layer and digital dispensing printing. The prototype has an optical modulation of ∼60%, a full-contrast lifetime tested beyond 20,000 cycles, an ultra-low power consumption (9.5 μW/cm 2 ), and the ability to provide spatiotemporally tunable information for potential AR applications.
研究领域:
材料科学
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显示技术
·
光学工程
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DOI
危险品化学品经营许可证（带存储）