FOOD CHEMISTRY
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2025
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IF 9.8
Linyan Wang,Meihong Ding,Qingzhong Lai,Haofang Wan,Yang Guan,Changyu Li
Astragali Radix is renowned for its dual use in medicine and food. This study presented a comprehensive approach that combines ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry, in-house library matching, fragment ion identification, molecular networking and collision cross section prediction to assess Astragali Radix quality. Based on this approach, 130 compounds were successfully characterised, categorised into 38 saponins, 68 flavonoids, 10 amino acids, 5 organic acids, and others. Additionally, 8 pairs of isomers were verified based on collision cross section measurements. Furthermore, Astragali Radix produced in the Gansu, Shanxi and Jilin provinces could be successfully discriminated using PLS-DA and OPLS-DA models. Twenty differential metabolites were identified including 8 flavonoids and 12 saponins such as astragaloside II, neoastragaloside I, astragaloside VII, astragaloside VI, soyasaponin I, calycosin-7- O - glucoside, formononetin-7- O -glucoside-6”- O -acetate, and ononin, which could be used as potential markers.
研究领域:
中药学
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分析化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Shiyao Hua,Jiayue Liu,Sijia Pu,Liang Zou,Lei Zhang,Peng Li
Comprehensive elucidation of the chemical constituents of Astragali Radix is crucial for its quality control and edible product development. Herein, online comprehensive two-dimensional hydrophilic interaction liquid chromatography × reversed phase liquid chromatography tandem high-resolution mass spectrometry was elaborately designed to separate, identify, and qualitatively compare various chemical components of Astragali Radix from two species. After selection and optimization of stationary phase, mobile phase, elution program and interface, an optimum method suitable for chemical components in Astragali Radix was established successfully. Under the optimized separation conditions, the orthogonality reached 99 %. A total of 212 compounds were identified and tentatively identified (198 from A. membranaceus var. mongholicus and 177 from A. membranaceus ). Additionally, the fragmentation pattern of flavonoids and saponins mass data in Astragali Radix have also been systematically studied. These findings not only unveiled multi-component complexity and diversity in two Astragalus species, but also proposed an innovative strategy for the rapid separation and identification of other complex systems.
研究领域:
中药学
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分析化学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
PHYTOCHEMICAL ANALYSIS
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2026
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IF 2.9
Xinyu Dong,Peng Zhou,Qiaomei Lou,Chang Li,Yu Du,Yu He
Purpose To research the therapeutic effect and compatibility mechanism of Astragali Radix-Safflower (ARSA) main effective parts in cerebral ischemia/reperfusion injury (CI/RI), especially pharmacokinetics and pharmacodynamics study. Method Nine formulas of three main effective parts (total flavonoids of Astragali Radix, total saponins of Astragali Radix and safflower yellow pigment) of ARSA were formulated according to L 9 (3 4 ) orthogonal design. The sample collection was performed via blood–brain dual-channel microdialysis, combined with LC–MS/MS to quantify six components (hydroxysafflor yellow A, astragaloside IV, calycosin-7-O-β-D-glucopyranoside, ononin, calycosin, and formononetin) of ARSA and five neurotransmitters (aspartic acid, 5-hydroxytryptamine, γ-aminobutyric acid, dopamine, and glutamate) associated with CI/RI. The entropy weight method and the partial least square method were applied to analyze data. Results The best compatibility prescription of the main effective parts of ARSA was total flavonoids of Astragali Radix 600 mg/kg, total saponins of Astragali Radix 140 mg/kg, and safflower yellow pigment 840 mg/kg, which had a better therapeutic effect and the largest total area under the curve than other formulas. Undergoing CI/RI, glutamate was primarily regulated among the five neurotransmitters, and the components with the greater impact on neurotransmitter modulation were hydroxysafflor yellow A and astragaloside IV. Conclusion The different compatibility of effective parts of traditional Chinese medicine would influence the pharmacokinetics parameters of each active component in CI/RI rats. Orthogonal compatibility and total statistical moment method provided a way to find the best compatibility. This study provided a pattern for the exploration of traditional Chinese medicine compatibility.
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
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2022
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IF 6.656
Yu Du,Chang Li,Shouchao Xu,Jiehong Yang,Haitong Wan,Yu He
Background Astragali Radix-Safflower combination (ARSC) is widely utilized in clinic to treat cerebral ischemia/reperfusion injury (CI/RI). Whereas, there is no in-depth research of the pharmacokinetics (PK) and pharmacodynamics (PD) analysis of ARSC after intragastric administration in rats with CI/RI. Purpose The purpose of this research is to investigate the PK characteristics of eight active ingredients (astragaloside IV, calycosin , calycosin-7-O-β-glucoside, formononetin , ononin , hydroxysafflor yellow A, syringin and vernine) of ARSC, and the regulation of neurotransmitters disorders, revealing the pharmacodynamic substance basis and the mechanism of ARSC in treating CI/RI from the molecular level. Methods We established a new method which based on blood-brain dual channel microdialysis (MD) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to continuously gather, and determine the components of ARSC and neurotransmitters related to CI/RI in vivo . The collected data were analyzed by sigmoid-E max function. The neurotransmitters primarily regulated in CI/RI rat were discussed by principal component analysis and the compound most associated with total pharmacodynamics was chosen by partial least squares regression . Results The validated LC-MS/MS method had specificity and selectivity to simultaneously analyze the concentration of eight active components of ARSC extract and five neurotransmitters of CI/RI rats. The recovery rates of brain MD probe and blood MD probe were stable within six hours. The MD probes recovery rates decreased with the increase of flow rates, but the solution concentration had little effect on the probes recovery rates. It was feasible to correct the recovery rates of probes in vivo by using reverse dialysis method. All eight active ingredients of ARSC could pass across the blood brain barrier after CI/RI. ARSC regulated the release of glutamate (Glu), γ-aminobutyric acid (GABA), dopamine (DA), 5-hydroxytryptamine (5-HT) and aspartic acid (Asp). Notably, astragaloside IV and hydroxysafflor yellow A might have better regulatory effect on neurotransmitters in comparison with other six measured components of ARSC, and Glu was the neurotransmitter mainly regulated in CI/RI rats. Conclusion The ARSC was able to treat CI/RI through ameliorating neurotransmitters disorders. There was a hysteresis between the peaked drug concentration and maximum therapeutic effect of ARSC. The drug effective concentrations range of ASIV, calycosin, calycosin-7-O-β-glucoside, syringin and vernine in blood microdialysate and calycosin, syringin, vernine in brain microdialysate were narrow, which need be paid attention in clinical use.
研究领域:
药理学
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神经科学
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分析化学
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中医药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）