MICROCHIMICA ACTA
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2022
·Vol. 189
·期 5
·1-10
·
IF 6.408
Ding Jinglei,Zhang Wenqi,Xue Fengying,Sun Yu,Yan Quizhu,Chen Yanwei,Shan Guiye
Gold nanoclusters (AuNCs) are widely used in the fluorescence detection of biomolecules in human serum due to their good fluorescence properties, low toxicity, and better biocompatibility. However, the weak fluorescence intensity of AuNCs limits the fluorescence detection of molecules within a wide concentration range. It is reported that coating AuNCs in ZIF-8 with adjustable pore size can effectively improve the fluorescence intensity of AuNCs and broaden the detection range. And the AuNCs wrapped in the gaps of ZIF-8 can prevent the fluorescence quenching caused by the aggregation of AuNCs. However, ZIF-8 has high crystallinity, poor dispersion, and easy deposition, which reduces the fluorescence stability of the detection system and affects the detection. Based on the above research, the highly hydrophilic polymer PEI was modified to the surface of ZIF-8, and a kind of nanocomposite material AuNCs/ChOx@ZIF-8/PEI was obtained by co-encapsulating AuNCs prepared with glutathione as a ligand and cholesterol oxidase (ChOx) into ZIF-8 modified with PEI. The composite material emits strong red light at 650 nm under the excitation of 395-nm light, and the system can sensitively detect cholesterol (Chol) in human serum. Compared with other materials, the PEI-modified composite has better solubility and stability, so the detection effect of Chol is better. Encapsulation of ChOx in the ZIF-8 shell can protect the enzyme and increase the local concentration of ChOx, thereby speeding up the reaction rate. Compared with free AuNCs/ChOx, the quenching rate of AuNCs/ChOx@ZIF-8/PEI system is doubled. Secondly, the addition of Fe 2+ to the detection process results in higher quenching rate and detection sensitivity. The system can detect Chol in the concentration range 0.1–2.4 μM, with a detection limit of 0.073 μM. The determination is a fast and sensitive strategy. In addition, the practicability of this assay in the detection of Chol in human serum has been verified. Due to its selectivity and sensitivity, it has potential application value in clinical diagnosis. Graphical abstract
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INORGANIC CHEMISTRY COMMUNICATIONS
·
2023
·
IF 3.8
You-Ping Jin,You Lu,Yue Gao,Qing Li
The sensitive and selective detection of glyphosate (Gly) in the environment is urgently need because it has significant implications for human health and environmental safety. In this work, a GSH-AuNCs/Cu 2+ sensing probe was fabricated via the coordination between Cu 2+ and GSH-AuNCs, which resulted in the suppression of the fluorescence of GSH-AuNCs. Glyphosate can competitively grasp Cu 2+ from GSH-AuNCs/Cu 2+ , leading to fluorescence recovery. Thereby, a simple and sensitive “turn-on” fluorescence strategy was developed for the quantitative detection of glyphosate. The linear range of glyphosate detection is 0.25 ∼ 1.25 μM, with a detection limit of 92.16 ng/mL. This method was demonstrated selectivity, fast response, and sensitivity, making it suitable for the quantitative determination of glyphosate in real water samples. Furthermore, a paper-based test strip was constructed using the sensing probe, which enabled on-site visual and semiquantitative detection of Gly. Overall, our strategy provides new opportunities for determination of organophosphorus pesticides in the environment.
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ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yifan Chen,Juan Qiao,Qianrong Liu,Mingming Zhang,Li Qi
Papain-stabilized gold nanoclusters ( [email protected] ) with a red fluorescence emission at 639 nm have been generated successfully in aqueous solution. The fluorescence of [email protected] could be quenched in the addition of Cu 2+ . Subsequently, a unique fluorescent probe based on [email protected] 2+ complex has been constructed for sensitive, selective and “turn-on” detection of D-penicillamine (D-pen). The sensing probe has exhibited a remarkable fluorescence enhancement in the presence of D-pen, which can be measured ranging from 30.0 μM to 2.0 mM (r 2 = 0.991) with the detection limit of 5.0 μM. Furthermore, the developed assay has been utilized to explore the metabolic process of D-pen in rats after intraperitoneal injection. As far as we concerned, the present study has reported the first analytical method for sensing serum D-pen in real time by using the fluorescent [email protected] 2+ complex. Our strategy paves a way for digging up the biological and clinical application of the fluorescent AuNCs.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yunjing Zang,Huifeng Zhang,Dawei Zhang,Qiong Jia
Fenitrothion (FTN), a widely used organophosphorus pesticide, raises significant concerns for food safety and environmental health, creating a demand for sensitive and rapid detection methods. In this study, as the first attempt, a red-emitting gold nanocluster (Arg/MTU-AuNCs) was synthesized for FTN detection. The introduction of arginine (Arg) and 4-hydroxy-2-mercapto-6-methylpyrimidine (MTU) significantly improves the fluorescence properties of AuNCs via Au-S and hydrogen bonds. After the addition of FTN, the fluorescence intensity of Arg/MTU-AuNCs varies with FTN concentration due to the static quenching, thus enabling its sensitive detection. The probe exhibits a linear detection range for FTN of 2.00-90.00 μg/L and a limit of detection of 0.72 μg/L. Furthermore, the probe exhibits high selectivity against common interfering substances (e.g., metal ions, anions, and coexisting pesticides). When used for analysis of real samples including tea, mulberry leaves, and oranges, the method achieves recoveries ranged from 90.50% to 108.67%. In addition, a fluorescent hydrogel sensing platform was constructed by combining the probe with agarose hydrogel, and portable detection of FTN was realized through a smartphone. This work offers an effective and portable approach for rapid FTN monitoring, with significant potential for food safety and environmental applications.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 13.2
Linlin Xie,Xingye Ma,Zheng Wang,Caifang Wang,Zhongxin Xiao,Ling-Dong Sun,Chun-Hua Yan,Chen-Jie Fang
Pyroptosis resulted from photodynamic therapy (PDT) plays a crucial role in triggering photoimmunotherapy (PIT). However, visible light-triggered PDT may rise phototoxicity, with low penetration into biological tissues. Crucially, the efficacy of PDT in eliciting pyroptosis remains suboptimal. To address these formidable challenges, we developed a O 2 -supplementing photosensitizer AuNCs@TAT that could efficiently induce pyroptosis upon irradiation with second near-infrared (NIR-II) light in hypoxic tumor microenvironment (TME). Replacing GSH with TAT resulted in a gold nanocluster AuNCs@TAT. The ratio of Au (I) to Au (0) shifted from 1: 0.26 in pristine AuNCs to 1: 1.21 in AuNCs@TAT, which contributed to enhanced catalytic activity of AuNCs@TAT. The superior catalase-like activity of AuNCs@TAT produced 1.9-fold higher O 2 than AuNCs. This O 2 self-supplying capability of AuNCs@TAT not only relieved hypoxic condition in TME but also significantly enhanced the efficiency of AuNCs@TAT to induce pyroptosis upon 1064 nm light irradiation. Western blotting indicated both remarkable upregulation of cleft Caspase-3 and GSDME-N, suggesting GSDME mediated pyroptosis. Further, release of pro-inflammatory cytokines and damage-associated molecular patterns (DAMPs) triggered a strong systematic immune response and reprogrammed the immunosuppressive microenvironment into an immunostimulatory state, which led to a significant suppress of tumor metastasis in mice. Overall, this work sets the stage for future advancements in the design of NIR-II photosensitizer with O 2 -supplementing capability and therefore offers an innovative framework and solid foundation for tumor PIT.
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 27
·5182-5190
·
IF 7.571
Ze Wang,Liang He,Songtian Che,Huiyuan Xing,Lin Guan,Zhe Yang,Xingchen Li,Andrei V. Zvyagin,Quan Lin,Wenrui Qu
As the most common cancer in men worldwide, prostate cancer has a serious impact on people's health. Until now, the development of a platform for integrating tumor targeting, imaging and an effective treatment for prostate cancer has remained challenging. Herein, a nano-system is designed to improve both diagnosis and treatment for prostate cancer. We successfully synthesized an AuNCs–LHRHa nano-system by combining PEI-modified gold nanoclusters (AuNCs) with LHRH analogues (LHRHa). Due to the good tunable optical properties and photothermal properties of AuNCs, the nano-system can not only achieve efficient fluorescence/computed tomography dual-mode imaging, but can also be used for photothermal therapy (PTT). After modifying the LHRHa antibody of a prostate tumor, AuNCs–LHRHa can be more effectively recognized by the gonadotropin-releasing hormone receptors (GnRH-R) on the membrane of RM-1 cells, enhancing the tumor cell uptake of the nano-system, improving the targeting accuracy and PTT therapy efficacy for prostate cancer. It is hoped that the nano-system, which combines dual-mode imaging and targeted therapy, will provide a promising strategy for the integration of FL/CT diagnosis and PTT therapy for GnRH-R positive prostate cancer.
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FOOD CONTROL
·
2021
·
IF 5.548
Hualin Guo,Yuhan Sun,Pengfei Ma,Imran Mahmood Khan,Nuo Duan,Zhouping Wang
Patulin (PAT) is a mycotoxin that exists in a wide range and is harmful to human health, which has aroused great attention to food safety. In this study, we established a novel assay to detect food samples contaminated by PAT. First of all, we proposed a truncation protocol based on the secondary structure of aptamer. By removing the stem-loop structure one by one, an optimized truncated PAT aptamer (P-30) was obtained. To the best of our knowledge, the mechanism between PAT and P-30 was elaborated in the assisting of molecular docking and circular dichroism for the first time. Furthermore, we developed a sensitive aptasensor platform to detect PAT in food samples. The aptamer modified with gold nanoclusters (AuNCs) was used as fluorescent probe, and BSA-directed synthesized MnO 2 nanoflakes (MnO 2 NFs) as fluorescence receptor. Additionally, fluorescence signal amplification strategy assisted by DNase Ⅰ successfully improved the sensitivity of aptasensor. The developed assay had a low detection limit of 8.5 ng/L, and can be effectively used in apple and grape juice samples. Thus, the established aptasensor shows a broad application prospect in PAT detection.
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ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Xiaozhe Xu,Juan Qiao,Nan Li,Li Qi,Shufeng Zhang
A new fluorescent probe based on ensemble of gold nanoclusters (AuNCs) and polymer protected gold nanoparticles (AuNPs) for turn-on sensing of l -cysteine was designed and prepared. The AuNCs were protected by bovine serum albumin and had strong fluorescence. The polymer protected AuNPs were synthesized by a facile in situ strategy at room temperature and could quench the fluorescence of AuNCs due to the Förster resonance energy transfer. Interestingly, it has been observed that the quenched fluorescence of AuNCs was recovered by l -cysteine, which could induce the aggregation of polymer protected AuNPs by sulfur group. Then the prepared fluorescent probe was successfully used for determination of l -Cys in human urines, which would have an evolving aspect and promote the subsequent exploration.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 26
·期 1
·139
·
IF 4.9
Yutong Huang,Simiao Zhang,Mei Zhou,Xiaokang Xu,Weiqing Sun,Jing Ma,Long Wu
Biogenic amines (BAs), produced in fish and seafood due to microbial contamination, pose significant health risks. This study introduces a novel ratiometric fluorescent probe, synthesized by integrating rhodamine 6G(R6G) and gold nanoparticles (AuNCs), for the sensitive and specific detection of BAs. The probe operates on the principle of BAs hydrolysis, catalyzed by diamine oxidase, to produce hydrogen peroxide (H2O2), which selectively quenches the fluorescence of AuNCs at 620 nm, while the fluorescence of R6Gat 533 nm remains unaffected. The ratio of I620/I553demonstrated excellent linearity with a wide dynamic range (1–1000 μM) and a low detection limit of 0.1 μM. Validation using grass carp samples showed high recovery rates (97.57% to 104.29%), confirming the probe’s efficacy and potential for practical application in food safety monitoring.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xuan Weng,Guanbo Wang,Hang Zhang,Hai Jiang
This study presents a titanium carbide MXene–gold nanocomposites (MXene–AuNCs)-based aptasensor for the sensitive detection of neuron-specific enolase (NSE). Laser-induced graphene electrodes (LIGEs) were fabricated using polyimide film as the three-electrode electrochemical sensing system. Specifically, MXene–AuNCs were immobilized on the working electrode to enhance both the electronic conduction and the immobilization capacity of the NSE aptamers (Apt). As a labeling material, MXene–AuNCs–Apt facilitated signal amplification, enabling highly sensitive detection. Under optimized conditions, this aptasensor exhibited an ultralow detection limit of 0.13 pg/mL for NSE, a wide detection range from 0.01 ng/mL to 100 ng/mL along with satisfactory reproducibility, repeatability, specificity and stability. Furthermore, the proposed aptasensor was successfully applied to measure NSE levels in serum samples. This work provides an efficient strategy for the fabrication of high-performance electrochemical biosensors in the preliminary estimates and diagnosis of lung cancer at early stages.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Sheng Gong,Fan Nie,Lingxuan Ma,Jiali Wan,Yu Yang,Wensheng Zhang,Jinchao Wei,Peng Li
The overuse of pesticides poses a significant threat to human health, driving the need for accurate and rapid strategies to identify various pesticides. A fluorescent nano-sensor (R-AuNCs@Aptamer) was developed by integrating an amino-modified carbendazim aptamer with red-colored gold nanoclusters (R-AuNCs). We subsequently engineered an aptamer-triggered turn-off-on Förster resonance energy transfer (FRET) platform, employing R-AuNCs@Aptamer in conjunction with tungsten disulfide nanosheets (WS 2 NS). In this setup, the carbendazim aptamer-stabilized red-emitting R-AuNCs (∼655 nm) served as the energy donor, while WS 2 NS acted as a fluorescence quencher. Upon the introduction of carbendazim, alterations in fluorescence intensity were monitored at 655 nm, facilitating the recognition of carbendazim. The resulting fluorescent nano-sensor displayed outstanding selectivity towards carbendazim, exhibiting a broad detection range of 0.1 to 1000 μg/L and remarkable sensitivity with a low limit of detection. It demonstrated reliability and practicality when applied to Notoginseng Radix et Rhizoma and Ginseng Radix et Rhizoma samples, with recoveries ranging from 90 % to 110 %. By changing the aptamer sequence, this sensor can be customized to detect different pollutants, exhibiting excellent tailorability and broad application potential.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Zhenjie Zheng,Siqi Cai,Mengqi Ma,Xiaoyi Chen,Zhanglin Weng,Da Huang,Bin Qiu,Zhenyu Lin,Fang Luo
ATT-AuNCs provide intrinsically efficient solid-state AIE emission without additional material engineering. • CRISPR/Cas12a enables efficient target-triggered molecular signal amplification. • The biosensor achieves a 0.37 pg·mL −1 detection limit for NDMA in processed meat samples. N -Nitrosodimethylamine (NDMA) is a potent carcinogenic small-molecule contaminant, and its trace-level occurrence necessitates sensitive and reliable detection methods. Herein, we developed an electrochemiluminescence (ECL) aptasensor integrating a solid-state aggregation-induced emission (AIE)-active gold nanocluster (Au NCs) luminophore with clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a-mediated molecular signal amplification for ultrasensitive NDMA detection. 6-Aza-2-thiothymine (ATT)-protected Au NCs (ATT-AuNCs) served as the ECL luminophore with triethylamine as the coreactant, while ferrocene (Fc)-labeled single-stranded DNA (Fc-ssDNA) acted as the ECL quencher. Upon NDMA recognition, target-induced strand displacement released a DNA activator, which activated the Cas12a/crRNA complex and triggered trans-cleavage of electrode-bound Fc-ssDNA. Cleavage of the ssDNA backbone disrupted the linkage between the Fc moiety and the electrode-anchored terminus, allowing Fc-containing cleavage products to detach from the electrode interface and thereby reducing Fc-mediated quenching. As a result, the ECL emission of ATT-AuNCs was restored, translating target recognition into an enhanced ECL response. The proposed sensor exhibited a linear range of 5 pg·mL −1 to 500 ng·mL −1 with a detection limit of 0.37 pg·mL −1 . By exploiting the intrinsically efficient solid-state AIE emission of ATT-AuNCs and CRISPR/Cas12a-mediated signal amplification, this strategy enables ultrasensitive NDMA detection. Download: Download high-res image (118KB) Download: Download full-size image
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