CHEMOSPHERE
·
2020
·
IF 5.778
Danping Wu,Fangfang Li,Quan Chen,Min Wu,Wenyan Duan,Qing Zhao,Bo Pan,Baoshan Xing
Significant degradation of organic contaminants in biochar sorption systems has recently been reported, demonstrating a promising potential application of biochar in pollution control . We hypothesized that the degradation of organic chemicals by biochar may be further enhanced under irradiation by UV light due to the photocatalytic activities of carbonaceous materials . Our results confirmed that UV conditions increased the degradation of rhodamine B (RhB) by up to three times compared to dark conditions. Washing biochar with ultrapure water further increased RhB photodegradation by up to ten times. This photodegradation increase was the highest for biochar produced at 1000 °C. HF treatment and the addition of biochar supernatant did not increase RhB photodegradation. Therefore, the biochar properties mediating RhB photodegradation may be related to solid particles, mostly the combination of graphite structures and organic functional groups. Based on XPS and FTIR analysis, we propose that the quinoid C O in biochar may play an essential role in RhB photodegradation. Further research on identifying the photoactive components of biochar will be fundamental for improved biochar manufacture and application.
研究领域:
环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2022
·Vol. 11
·期 3
·1351-1360
·
IF 14.511
Deqi Wang,Jie Liu,Tianyu Tan,Shishuo Liu,Guozhen Xu,Zhimiao Zhang,Yang Qin,Fengsheng Li
Boron (B) is the most suitable fuel for rocket ramjet. However, boron oxide (B2O3) on the surface of B causes many problems in its application. B2O3 cross-links with hydroxyl prepolymer, which reduces the processibility of the system. Moreover, B2O3 hinders contact between the oxidation component and internal active B. Consequently, B is difficult to ignite, and its reactivity and combustion efficiency are limited. To solve these problems, a dual-core–shell structure, B@LiF@AP, B embedded with LiF as core and AP as shell, has been designed and prepared. Its multi-effect synergies, improving processibility, protecting B from oxidation by AP before burning, decomposing B2O3, and improving reactivity and combustion efficiency, have been confirmed. Compared with the mixed sample of B and AP (B–AP), the heat release of B@LiF@AP increased by 211.10%. The combustion efficiency of B@LiF@AP increased by 42.60% more than that of B@AP. The ignition test showed that its ignition energy was reduced by 37.89% and the flame temperature increased by 26.27% than that of B–AP. The application and development of boron-based rocket ramjet will be accelerated because the application problems of B are solved by B@LiF@AP.
研究领域:
材料科学
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航空航天工程
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DOI
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 27
·275604
·
IF 3.953
Kai Cheng,Jie Zhou,Yujie Zhao,Yu Chen,Lan Ming,Da Huang,Rui Yang,Zhenyu Lin,Daozhen Chen
Herein, an amphiphilic block copolymer CD44-targeting peptide-conjugated polyethylene glycol-block-hydroxyethyl starch-block-poly (L-lactic acid) (CD44p-conjugated PEG-b-HES-b-PLA) are synthesized, which could self-assemble into the pH-responsive and CD44-targeting polymer micelles against breast cancer cells MDA-MB-231. Emodin (Emo) is a natural anthraquino with pharmacological activities in anti-tumor effects. However, Emo suffers from poor water solubility, low biocompatibility, rapid systemic elimination, and off-target side effects, resulting in unsatisfactory treatment outcomes. Nanotechnology-based drug delivery systems have proven great potential for cancer chemotherapy. The constructed polymeric micelles Emo@CD44p-PM have exhibited an average size of 154.5 ± 0.9 nm characterized by DLS and TEM. Further, the Emo@CD44p-PM have effective Emo-loading capacity, good thermal stability, and pH responsiveness. Intracellular uptake study shows the enhanced cellular internalization of Emo@CD44p-PM due to the increased exposure of CD44p enhances the cellular internalization of Emo@CD44p-PM effectively. Furthermore, the in vitro results showed Emo@CD44p-PM has been observed good biocompatibility and anti-tumor effects. Therefore, the polymeric micelles Emo@CD44p-PM provide a promising delivery strategy of targeted therapy for breast cancer.
研究领域:
纳米技术
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药学
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癌症研究
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高分子化学
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产品关联
DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
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IF 4.7
Tingbiao Wu,Deyao Li,Qiuyu Chen,Dezhi Kong,Hongxuan Zhu,Hefeng Zhou,Qingwen Zhang,Guozhen Cui
Ginkgolide B (GB), a compound derived from Ginkgo biloba , exhibits significant cardioprotective properties, although its precise molecular target has yet to be identified. In this study, we synthesized a biotin-labeled GB probe (GB-biotin) to identify the molecular targets of GB. Our experiments demonstrated that treatment with GB or GB-biotin reduced mitochondrial injury, restored mitochondrial membrane potential, and decreased cell apoptosis in a concentration-dependent manner. Additionally, GB increased mitochondrial oxygen consumption rate, indicating improved mitochondrial bioenergetics. Proteomic analysis revealed that the voltage-dependent anion channel 1 (VDAC1) is a key protein that interacts with GB-biotin. This finding was further confirmed through cellular thermal shift assay (CETSA) and molecular docking, which revealed hydrogen bond formation between GB and VDAC1. Furthermore, overexpression of VDAC1 diminished the protective effects of GB, highlighting the crucial role of VDAC1 in GB-mediated cardioprotection. These findings identify VDAC1 as a therapeutic target for GB in vitro , providing valuable insights into the cardioprotective mechanisms of GB and the development of novel cardioprotective strategies.
研究领域:
药理学
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分子生物学
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心血管科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE INDIAN CHEMICAL SOCIETY
·
2024
·
IF 3.2
Lijun Jin,Lixia Jin,Haiyang Liu,Zhe Wang,yuguang Lv
Rhodamine B, hydrazine hydrate, and HCl generated the intermediate LBXJ. Next, LBXJ, chromone 3-formaldehyde, and 100 % ethanol prepared the probe Y2ST. NMR, IR, and MS. described the intermediate and probe structures. Following the determination of the structure, tests were conducted on the response time, pH value, linearity, detection limit, accuracy, coordination ratio, and recovery of samples. Additionally, the fluorescence selectivity of ions was compared. The rate of Zn 2+ detection in zinc gluconate was 96.1 % at 5 × 10 −6 mol/L--5 × 10 −5 mol/L, while the detection limit of Y2ST was 0.7 × 10 −6 mol/L. It can be applied to Zn 2+ content determination.
研究领域:
分析化学
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生物化学
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产品关联
DOI
高影响因子
INFECTION GENETICS AND EVOLUTION
·
2025
·
IF 2.6
Huanhuan Zhang,Fengjun Sun,Wei Feng,Yan Qian
Objectives This study investigated the mechanisms underlying polymyxin B (PMB)-induced resistance and examined the role of PMB in combination with amikacin (AMK) in delaying the development of resistance. Methods In vitro continuous induction of three Klebsiella pneumoniae strains was performed using PMB alone or in combination with AMK, and variations in the minimum inhibitory concentration (MIC) were determined via the microdilution method. The Kp81 strain, which presented the most significant delay in resistance development, was selected for analysis of the expression of relevant genes via qRT–PCR. Homologous recombination techniques were employed to construct nlpE and cpxR gene knockout and complemented Kp81 strains, and the expression levels of these genes, as well as changes in the MIC following continuous induction, were assessed. Additionally, the biofilm-forming abilities of the strains were analyzed via crystal violet staining and confocal laser scanning microscopy. Results The results indicate that the combination of PMB and AMK can delay the development of resistance to PMB in K. pneumoniae . qRT–PCR analysis demonstrated a significant increase in the expression of phoP , nlpE , cpxR , and acrA in the resistant strain Kp81·R, whereas these genes were significantly downregulated in the resistant delay strain Kp81·DR. Notably, after 20 h of PMB treatment, the expression of nlpE was markedly elevated in the nlpE knockout strain. cpxR exhibited synchronized dynamic changes with nlpE in both the nlpE knockout and complemented strains, whereas acrA showed a similar expression pattern in the cpxR knockout and complemented strains. Additionally, the absence of nlpE or cpxR was found to delay PMB resistance. Furthermore, biofilm formation was significantly increased in both the resistant strains and those treated with PMB, while the combination of PMB with AMK inhibited biofilm formation. Conclusions The combination of PMB and AMK can delay the development of resistance in K. pneumoniae to PMB. The nlpE gene, as a key regulatory factor, can influence PMB resistance by modulating the CpxA/R two-component system and biofilm formation.
研究领域:
微生物学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Jing Huang,Jiang Peng,Junqing Zeng,Liping Zheng,Huajie Chen
Despite the important advances on B/N co-doped porous carbons (BNCs), integrating high B/N-doping content and hierarchical porosity into carbon nanosheets remains a challenging task for supercapacitor application. Herein, we report a novel BNC nanosheet with both expected properties through one-step H 3 BO 3 -mediated carbonization/activation of a newly-developed N/O-rich polymer (DPP). In such a mixture system, an in-suit crossing-linked DPP@H 3 BO 3 composite is formed due to the formation of multiple intermolecular hydrogen bonds between the H 3 BO 3 and the ester groups of DPP. The improved thermal stability for such a composite enables the fixation of more B-/N-bearing species in the carbon skeleton during carbonization. Particularly the nanosheet-like BNC-850 shows a rather high B/N content (6.63/7.27 at.%), a large S BET value (766.5 m 2 g −1 ), and a hierarchical porosity, all of which are in favor of electrochemical performance in both aqueous supercapacitors and zinc-ion capacitors (ZICs). In 6 M KOH electrolyte, the BNC-850 electrodes deliver an impressive capacitance of 341.5 F g −1 at 0.5 A g −1 , a high capacitance retention of 79.2 % at 20 A g −1 , and a good cycling stability (95 % capacitance retention over 10,000 cycles at 5 A g −1 ). The energy density of BNC-850-based ZICs is as high as 126.0 Wh kg −1 at 459.0 W kg −1 , exhibiting an outstanding superiority compared to the reported carbon-based ZICs. This work offers a facile strategy for accessing BNCs with high B/N-doping contents, and highlights the importance of such materials in high-energy-density ZICs.
研究领域:
材料科学
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电化学
·
能源存储
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产品关联
DOI
高影响因子
PHARMACOLOGICAL RESEARCH
·
2022
·
IF 10.334
Jiale Zhu,Zhiwei Jin,Lei Yang,Caili Zhao,Jianping Hu,Jinhu Chen,Yubao Han,Pei Yu,Jun Luo,Lingyi Kong,Chao Zhang
Promoting angiogenesis in the ischemic penumbra is a well-established method of ischemic stroke treatment. Ginkgolide B (GB) has long been recognized for its neuroprotective properties following stroke. As previously reported, it appears that stroke-induced neurogenesis and angiogenesis interact or are dependent on one another. Although the pharmacodynamic effect of GB on cerebral blood flow (CBF) following ischemic stroke has been reported, the molecular mechanism underlying this effect remains unknown. As such, this study sought to elucidate the pharmacodynamic effects and underlying mechanisms of GB on post-stroke angiogenesis. To begin, GB significantly increased the proliferation, migration, and tube formation capacity of mouse cerebral hemangioendothelioma cells (b.End3) and human umbilical vein endothelial cells (HUVEC). Additionally, GB significantly improved angiogenesis after oxygen-glucose deprivation/reperfusion (OGD/R) in endothelial cells. The dynamics of CBF, brain microvascular neovascularization and reconstruction, and brain endothelial tissue integrity were examined in middle cerebral artery occlusion (MCAO) mice following GB administration. Through label-free target detection techniques, we discovered for the first time that GB can specifically target Creatine Kinase B (CKB) and inhibit its enzymatic activity. Additionally, we demonstrated through network pharmacology and a series of molecular biology experiments that GB inhibited CKB and then promoted angiogenesis via the CCT/TRiC-SK1 axis. These findings shed new light on novel therapeutic strategies for neurological recovery and endothelial repair following ischemic stroke using GB therapy.
研究领域:
神经学
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药理学
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分子生物学
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细胞生物学
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卒中研究
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2025
·Vol. 39
·期 2
·e6070
·
IF 1.7
Li Li,Dan Yu,Wen-Fei Sun,Qi-Zhong Jin,Xing-Jie Chang,Qian Shi
Ginkgolide B (GB) is the main active ginkgolide in Ginkgo biloba leaves extract. Pharmacological study suggested that GB exhibits protective effect on nervous system impaired and can be used in the treatment of dementia, cerebral insufficiency or related cognitive decline. However, the information on pharmacokinetics of GB in vivo was limited. In this study, a sensitive LC–MS/MS analytical method was developed to accurately determinate the concentration of GB in dog plasma and applied to the pharmacokinetic study of GB in dogs after bolus injection of GB. A portion of 100 μL dog plasma was pretreated by liquid–liquid extraction using 1 mL of ethyl acetate. The GB and IS were separated on a Waters ACQUITY HSS T3 column using acetonitrile and water containing 0.1% formic acid as mobile phase, at a flow rate of 0.4 mL/min. Multiple-reaction monitoring (MRM) mode was used for quantitative analysis of GB and tolbutamide (internal standard) in negative electrospray ionization. GB showed excellent linearity with correlation coefficient > 0.99 over the concentration range of 1–5000 ng/mL. The intra and interday RSD% were less than 7.4%, while the RE% ranged from −6.1% to 9.6%. The mean extraction recovery was > 83.1%. The validated method was further successfully applied to pharmacokinetic study of GB in dog plasma. Dose-proportional pharmacokinetics of GB were observed after bolus injection administration in dogs. This comprehensive study of the pharmacokinetics of GB in dogs will provide useful information for its further development in clinic.
研究领域:
药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 10
·7772-7779
·
IF 3.289
Huiping Zhao,Yafang Zhang,Guangfang Li,Fan Tian,Han Tang,Rong Chen
Loose-packed flower-like BiOCl hierarchical nanostructures (HNs) assembled by thin nanosheets were successfully synthesized via a facile solvothermal method. Based on the matchability analysis of band structure of BiOCl and the molecular orbitals of the dyes, the RhB dye-sensitized heterogeneous BiOCl HNs system was established for MO degradation under visible light irradiation. The mechanism of MO degradation over RhB-sensitized BiOCl was also discussed according to the photocurrent and photoluminescence results. It was found that MO degradation efficiency over RhB-sensitized BiOCl HNs was obviously improved due to the generation of superoxide radicals (˙O2−) through the reduction of surface-adsorbed O2 molecules by the injected electrons on the conduction band of BiOCl HNs from the excited RhB molecules. This work may provide insight on better understanding the organic dye photodegradation process in RhB dye-sensitized BiOCl heterogeneous systems and explore a dye-sensitized semiconductor photocatalyst strategy for environmental remediation.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
MOLECULES
·
2015
·Vol. 20
·期 11
·20031-20041
·
IF 2.416
Jie Zhao,Ting Geng,Qi Wang,Haihong Si,Xiaoping Sun,Qingming Guo,Yanjing Li,Wenzhe Huang,Gang Ding,Wei Xiao
Ginkgolide B (GB), an important active constituent ofGinkgo bilobaextract, has been used in clinical applications for the treatment of dementia, cerebral insufficiency or related cognitive decline. To investigate the main pharmacokinetic characteristics of three different GB formulations in beagle dogs, a simple, specific and sensitive LC-MS/MS method was established and validated. The separation of the analytes was achieved on an Agilent Eclipse Plus C18column (1.8 μm, 2.1 × 50 mm) with a mobile phase consisting of water and acetonitrile. The flow rate was set at 0.4 mL/min. Quantitation was performed using multiple reaction monitoring (MRM) in negative ion mode, with the transitions atm/z(Q1/Q3) 423.1/367.1 for GB andm/z269.3/170.0 for IS. The linear calibration curve of GB was obtained over the concentration range of 2–200 ng/mL. The intra- and inter-day precisions were <15% and the accuracies were within ±12.7%. The validated method was applied to compare the pharmacokinetic characteristics of GB in healthy beagle dogs after oral administration of three formulations (HME08, GB capsule prepared by hot-melt extrusion technology; LL06, GB pellet prepared by liquid layer technology; conventional GB tablet). The Cmaxvalues of GB from different formulations in beagle dog plasma were 309.2, 192.4 and 66.6 µg/L, and the AUC values were 606.7, 419.1 and 236.2 µg/L·h, respectively. The data suggested that the exposure level of GB from HME08 and LL06 in beagle dog plasma was greatly improved compared with conventional tablets. This study should be helpful for the design and development of oral GB preparations.Keywords:ginkgolide B;hot-melt extrusion;liquid layer;beagle dog plasma;pharmacokinetics;LC-MS/MS
研究领域:
药理学
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药物分析
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 6
·1847-1859
·
IF 2.647
Li Chunxia,Guo Xin,Du Hongmei,Zhao Jinsheng,Liu Lixia,Yuan Qing,Fu Chonggang
Two novel donor-acceptor (D-A)-conjugated polymers, poly[(thiophene-2,5-yl)-((benzo[1,2-b:6,5-b′]dithiophene-4,5-dione)-2,7-yl)] (PTDT) and poly[((2,3-dihydrothieno[3,4-b] [ 1 , 2 ]dioxine)-5,7-diyl)-((benzo[1,2-b:6,5-b′]dithiophene-4,5-dione)-2,7-diyl)] (PTDO), have been synthesized with the same acceptor unit of benzo[1,2-b:6,5-b′]dithiophene-4,5-dione and different donor units of thiophene (Th) and 3,4-ethylenedioxothiphene (EDOT), respectively. And then, two composites (PTDT@C and PTDO@C) are made by coating the polymers on the surface of active carbon with an in situ method, which are used as the electrode materials of lithium-ion battery. The reversible capacities of PTDT@C and PTDO@C are 246.9 and 322.3 mAh g −1 at 0.1 A g −1 after 300 cycles running, respectively. By deducing the capability contribution of pure carbon, the theoretical capacities of the pure polymers are calculated to be 596 and 991 mAh g −1 , respectively, for PTDT and PTDO, which correspond to the lithium storage number of 6.7 and 13.3, respectively, for the least repeating unit of the polymers. With the help of theoretical calculations, the underlying mechanisms for the different electrochemical performances of the polymers are discussed and revealed. The structure-activity relationship discussed in this work may provide the design principle for the construction of the D-A-type polymers used as high-capacity electrode materials for organic rechargeable batteries.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）