BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2021
·Vol. 85
·期 8
·1809-1817
·
IF 2.043
Li Qiang,Zhai Chunmiao,Wang Guodong,Zhou Jia,Li Weiguang,Xie Liquan,Shi Zhanli
Ginsenoside Rh1 (Rh1) has anti-inflammatory effects in asthma mice, but the underlying mechanism remains unclear. BALB/c mice were sensitized and challenged with ovalbumin (OVA) to construct asthma model. Mice received Rh1 or tiotropium bromide 0.5 h before OVA challenge. Airway morphology and airway remodeling were assessed by HE staining and Masson's trichrome staining, respectively. Th1/Th2 cytokines in serum or broncho alveolar lavage fluid (BALF) were measured by ELISA kits. Rh1 significantly alleviated the lung resistance and airway resistance, and reduced the number of total inflammation cells, eosinophils, neutrophils, and lymphocytes in BALF of the asthmatic mice. The morphological changes and collagen deposition of airway were also reduced by Rh1 in asthmatic mice. The increase of Eotaxin, IL-4, IL-5, IL-13, and IL-33 and the decrease of IL-12 and IFN-γ in both BALF and serum of OVA exposed mice were reversed by Rh1. Rh1 attenuates OVA-induced asthma in the mice model by regulating Th1/Th2 cytokines balance.
研究领域:
免疫学
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药理学
·
呼吸病学
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DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2023
·
IF 3.9
Yanping Xie,Xuekun Ma,Abdul Waheed,Muhammad Adnan,Ning Xie,Gang Liu
Cellobiose dehydrogenase (CDH) catalyzes the oxidation of cello-oligosaccharides and transfers electrons to oxygen or other electron receptors, such as lytic polysaccharide oxygenases (LPMOs). Cellobiose dehydrogenase has the potential to be used as an electron donor for LPMOs. Therefore, it is important to obtain CDHs under reaction conditions that are compatible with those of cellulases and LPMOs. Here, a CDH gene from Myceliophthora thermophila ( Mt CDH-A) was constitutively expressed in Trichoderma reesei , and its ability to donate electrons to the LPMO was validated. Mt CDH-A has an optimal pH of 6.0 and maintains relatively high activity over a wide pH range. The optimal temperature was 60°C, and it remained stable at 50°C for a long time. These enzymatic properties were compatible with the catalytic conditions of fungal LPMOs and cellulases. When Mt CDH-A was used as an electron donor, the synergistic effect of LPMO and cellulase improved the degradation efficiency of phosphoric acid-swollen cellulose by 141%. This work provides a novel insight into finding a stable electron donor for LPMOs and a new direction for finding more efficient thermostable CDHs in newly identified fungal species. © 2023 Society of Industrial Chemistry and John Wiley & Sons Ltd.
研究领域:
生物化学
·
酶学
·
微生物学
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DOI
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2021
·Vol. 16
·期 1
·193-203
·
IF 4.102
Miaojia Song,Jianping Lu,Yu Zhang,Yongming Sun,Zhenhong Yuan,Zhongming Wang
Furfural (FF) is an important bio-based platform compound derived from lignocellulose. It can be converted into many industrially valuable chemicals, a typical one being methyl-2-furoate (MF). At present, MF production depends largely on vigorous one-step chemical catalysis (oxidative esterification), in which expensive catalysts (precious metals) and a high temperature (60–120 °C) are required. To reduce the costs of this procedure, a mild, green, and cheap chemoenzymatic process is proposed where FF is initially chemically oxidized to furoic acid (FA), and then FA is enzymatically esterified to MF. Neither step requires an expensive catalyst, and both occur at low temperatures (30–50 °C). For the chemical oxidation process, the optimized FA yield of 83.7% was obtained at 40 °C with an FF/BTIC (1,3-bis(2,4,6-trimethylphenyl) imidazolium chloride) molar ratio of 0.18, 10 mL N -methyl pyrrolidone, and 0.1 MPa oxygen pressure. For the enzymatic esterification, the optimized MF yield reached 80.6% at 50 °C with 50 mg•mL −1 enzyme loading and an alcohol/acid ratio of 7:1. Kinetic studies demonstrated the oxidation to be a sixth-order reaction, and the esterification to be an endothermic reaction. For both oxidation and esterification reactions, the relationship between the rate constant and temperature agreed well with the Arrhenius equation. For esterification, the relationship between the equilibrium constant and the temperature also agreed with the Van't Hoff equation. A 67.5% MF yield was obtained when sequential oxidation and esterification were initiated from FF. The development of chemoenzymatic processes for MF production from FF is effective and has potential value in decreasing the production cost of MF. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd
研究领域:
化学工程
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生物技术
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绿色化学
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DOI
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2026
·
IF 3.8
Zihao Zhang,Yu Zhang,Wei Liu,Wen Wang,Victor Chernyshev,Cuiyi Liang,Shiyou Xing,Xuesong Tan,Wei Qi
Furfural (FF), an important biomass platform compound, is extensively utilized in the synthesis of high-value-added chemicals, fuels, resins, and pharmaceuticals. To achieve the efficient conversion of xylose into FF, we systematically screened a series of metal salt catalysts in a homogeneous methanol–water system and thoroughly investigated their catalytic performance and reaction mechanisms. The results demonstrated that AlCl 3 exhibited the optimal catalytic activity in this reaction, achieving >99% xylose conversion and a 77.3% FF yield under the conditions of 160 °C and 2 h of reaction. Kinetic studies indicated that the conversion of xylose to intermediates was the rate-determining step, with an apparent activation energy of 42.97 kJ mol −1 . Mechanistic analysis further revealed that AlCl 3 possesses both Lewis and Brønsted acid sites, which synergistically catalyze the multi-step reactions – including isomerization, ring-opening, dehydration, and cyclization – of xylose to form FF. This study develops a green and efficient catalytic system prototype with low cost, which not only clarifies the structure–performance relationship of AlCl 3 and the dual function of the methanol–water system, but also provides a theoretical foundation and technical basis for the sustainable production of biomass-based FF.
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DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2025
·
IF 1.3
Zhai Zhenyu,Zhang Yi-Heng P Job
Formate oxidase (FOX) has applications in enzymatic assays, pollutant biodegradation, and bioorganic chemistry. However, its sources are few and none of them have significant activity above pH 7. We performed bioinformatic mining of FOX and identified 579 potential sequences. Two FOXs from Aspergillus nomiae (AnFOX) and Trichophyton rubrum (TrFOX) were expressed in E. coli. AnFOX had an alkalinity-tolerant pH activity range. It not only had a Km value for formate one order of magnitude lower, but also had far higher catalytic activity under non-acidic conditions. Spectroscopic and mass spectrometry analyses found that AnFOX contains an 8-formyl FAD cofactor. AnFOX enabled the H2O2-dependent sulfoxidation of thioanisole over pH 6-8. Structural analysis and kinetic studies revealed that the acidic residue E142, positioned on the surface adjacent to the isoalloxazine ring of flavin, could contribute to the unique pH preference. This novel FOX could greatly expand the applicability under non-acidic conditions.
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产品关联
DOI
PubMed
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2025
·
IF 1.3
Yang Zhongyi,Gong Ruhai,Mimata Yoshiharu,Ye Shaosong,Ji Wei,Ye Wenxiu
A key tricarboxylic acid cycle metabolite, malate, accumulates in leaves during dehydration and induces stomatal closure by recruiting cytosolic Ca2+, activating a SLOW ANION CHANNEL-ASSOCIATED 1, and promoting reactive oxygen species. However, the effects of malate on stomatal opening and its underlying molecular mechanisms remain poorly understood. Our study revealed that, among TCA cycle metabolites, malate specifically inhibited light-induced stomatal opening in both grapevine and Arabidopsis. We demonstrated that SLAC1 was required for malate's inhibitory effects. The inhibition by malate was disrupted by Ca2+ signaling inhibitors. Additionally, malate signal was mediated by G-proteins, which regulate the production of second messengers. ROS production was abolished when G-proteins were inhibited. These findings show that malate efficiently maintains stomatal closure by not only inducing stomatal closure but also inhibiting stomatal opening. The inhibition of stomatal opening by malate is mediated through the activation of SLAC1 and the G-protein signaling cascade.
研究领域:
植物生理学
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分子生物学
·
细胞信号传导
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产品关联
DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2024
·
IF 3.2
Haonan Zhu,Shanshan Yan,Yansheng Wu,Hao Xu,Haoran Chen,Hong Zhang,Xin Guo,Xun Hu,Shu Zhang,Wenran Gao
In this study, biochars with different structures were prepared through HNO 3 oxidation and secondary pyrolysis to study the relationship between the structure of biochar and its selectivity for the adsorption of light and heavy aromatics in bio-oil. The results showed that the adsorption rates of biochar with different structures ranged from 2.90 to 4.00 g bio-oil per g biochar. The influence of pore structure was dominant, followed by the influence of O-containing functional groups and the degree of graphitization. The higher the adsorption capacity of biochar for bio-oil, the smaller the concentrations of the light aromatic model compounds in the absorbed bio-oil (ABO). Among the five light aromatics, biochar has the best adsorption selectivity for dihydroxybiphenyl and the worst for eugenol and propyl phenol. This is attributed to the diphenyl ring structure of dihydroxybiphenyl, which makes it more susceptible to adsorption, and other light model compounds only have one benzene ring. In summary, biochar demonstrates better adsorption selectivity for heavy aromatics than light aromatics. The more developed the pore structure is, the more enriched O-containing functional groups are, and the higher the graphitization degree of biochar is, the better the selective adsorption of aromatic compounds in bio-oil.
研究领域:
环境科学
·
能源科学
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DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2024
·
IF 1.4
Chen Xue,Kang Yulai,Tang Chunhua,Zhang Lili,Guo Lu
Foam cells are primarily formed through scavenger receptors that mediate the uptake of various modified low-density lipoproteins (LDL) into cells. In addition to the receptor-dependent pathway, macropinocytosis is an essential non-receptor endocytic pathway for vascular smooth muscle cells (VSMCs) to take up lipids. However, the molecular mechanisms underlying this process remain unclear. Primary cultured VSMCs were stimulated with 200 ng/ml lipopolysaccharide (LPS) and 200 μg/ml native LDL (nLDL). We observed a significant increase in TLR4 protein expression and a significant activation of macropinocytosis, which correlated with the highest uptake of nLDL and intracellular lipid deposition in WT VSMCs. However, macropinocytosis was inhibited and lipid accumulation decreased after treatment with macropinocytosis inhibitors and Syk inhibitors in WT VSMCs. Consistently, TLR4 knockout significantly suppressed macropinocytosis and lipid droplets accumulation in VSMCs. Taken together, our findings suggest a critical role of TLR4/Syk signaling in promoting receptor-independent macropinocytosis leading to VSMC-derived foam cells formation.
研究领域:
心血管生物学
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细胞生物学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2024
·Vol. 18
·期 6
·1994-2004
·
IF 3.2
Wanling Shen,Haihang Zhu,Xiaoman Cheng,Xin Li
Mesoporous niobium-based solid acid catalysts were successfully synthesized using the soft template method with sodium lignosulfonate (SLS) as the template. In most studies the template is calcined to form mesopores after hydrothermal synthesis; however, in this study SLS was retained to produce niobium-carbon composite catalysts. The catalysts obtained were characterized by X-ray diffraction, nitrogen adsorption–desorption, Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and static contact angle measurements. The catalytic activity of the solids was also investigated in the conversion of glucose to 5-hydroxymethylfurfural (HMF) using pure water as the solvent. Under optimal conditions, niobium-carbon composite catalyst achieved a glucose conversion yield of 65.1% and an HMF yield of 42.1%. The enhanced catalytic activity was attributed to the timely extraction of the HMF by the carbonized SLS.
研究领域:
催化化学
·
材料科学
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产品关联
DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2024
·
IF 1.4
Wu Tao,Wu Ji,Liu Li,Song Hongping
Lipopolysaccharide (LPS) causes inflammatory cholestasis in sepsis. We investigated the role of PDZK1 in the repression of ABC transporters in LPS-induced cholestasis. Lentiviral gene transfer of PDZK1 to rats was conducted to explore its influence on cholestasis induced by LPS. And the effect of lentivirus-mediated shRNA targeting PDZK1 on ABC transporters in rat liver BRL-3A cells was evaluated. Lentiviral vector encoding rat PDZK1 was administered to rats by tail intravenous injection. Obviously elevated serum total bile acid (TBA) level and liver biochemical markers in cholestatic rats were decreased by the Lv-PDZK1 delivery. Also Lv-PDZK1 delivery stimulated the suppressed hepatic ABC transporters expression. In vitro, after the lentiviral vector LV3/PDZK1 shRNA transfection, no expression of PDZK1 and mild expression of ABC transporters were detected in BRL-3A cells by Western blotting. Our results indicate that the lentiviral-mediated hepatocyte PDZK1 expression ameliorates LPS-induced cholestasis in rats by rescuing hepatic ABC transporters expression.
研究领域:
分子生物学
·
肝病学
·
基因治疗
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产品关联
DOI
PubMed
高影响因子
Biofuels Bioproducts & Biorefining-Biofpr
·
2024
·
IF 3.2
Yue Ouyang,Ying Liu,Longjin Tian,Yongyu Ding,Guozhi Fan,Cheng Pan,Qunpeng Cheng
Anaerobic digestion is a promising technology for the treatment of food waste (FW) but it requires optimization to improve its performance. This study investigated whether combining the addition of biochar with lipase pretreatment could improve the anaerobic digestion of FW. The results showed that biochar stimulated the release of soluble substances, resulting in an increase in the methane yield of groups with added biochar by 16.24% to 19.36% in comparison with the group without biochar. Lipase pretreatment substantially shortened the fermentation time required to complete the anaerobic digestion, from 15 to 9 days. Lipase pretreatment further improved microbial abundance and promoted the enrichment of functional microbes in the anaerobic digestion of FW mediated by biochar. It also changed the methane production pathway from acetotrophic to hydrogenotrophic, thereby ensuring the stability of the anaerobic digestion system in the presence of a high ammonia nitrogen concentration.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
高影响因子
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
·
2012
·Vol. 76
·期 6
·1091-1097
·
IF 1.276
Yue-Xiu SI,Zhi-Jiang WANG,Daeui PARK,Hyoung Oh JEONG,Sen YE,Hae Young CHUNG,Jun-Mo YANG,Shang-Jun YIN,Guo-Ying QIAN
We studied the inhibitory effects of isorhamnetin on mushroom tyrosinase by inhibition kinetics and computational simulation. Isorhamnetin reversibly inhibited tyrosinase in a mixed-type manner at Ki=0.235 ± 0.013 mM. Measurements of intrinsic and 1-anilinonaphthalene-8-sulfonate(ANS)-binding fluorescence showed that isorhamnetin did not induce significant changes in the tertiary structure of tyrosinase. To gain insight into the inactivation process, the kinetics were computed via time-interval measurements and continuous substrate reactions. The results indicated that inactivation induced by isorhamnetin was a first-order reaction with biphasic processes. To gain further insight, we simulated docking between tyrosinase and isorhamnetin. Simulation was successful (binding energies for Dock6.3: −32.58 kcal/mol, for AutoDock4.2: −5.66 kcal/mol, and for Fred2.2: −48.86 kcal/mol), suggesting that isorhamnetin interacts with several residues, such as HIS244 and MET280. This strategy of predicting tyrosinase interaction in combination with kinetics based on a flavanone compound might prove useful in screening for potential natural tyrosinase inhibitors.
研究领域:
生物化学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）