JOURNAL OF APPLIED POLYMER SCIENCE
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2014
·Vol. 132
·期 9
·
IF 1.64
Yan-Yan Hu,Jing Zhang,Qi-Chen Fang,Dong-Mei Jiang,Chu-Cheng Lin,Yi Zeng,Ji-Sen Jiang
A novel semi-interpenetrating poly(2-hydroxyethyl methacrylate) (pHEMA) based polyelectrolyte hydrogel [p(HEMA- co -METAC)/PEG] was prepared by copolymerizing HEMA with the cationic monomer 2-methacryloyloxyethyltrimethyl ammonium chloride (METAC) in the presence of polyethylene glycol (PEG) with different content and molecular weight (MW 4000 and 400). The chemical structure of the gels was confirmed by FT-IR spectroscopy, morphology study was performed by scanning electron microscope (SEM), thermal stability was revealed by thermogravimetric analysis (TGA), and the mechanical properties were determined by electronic universal testing machine. Swelling studies showed introduction of cationic monomer METAC led to high water content, and the obvious salt and pH sensitive properties were observed which proved the smart behavior of the semi-interpenetrating polymer networks (IPNs) gels. In addition, the effect of temperature and some important biological solution on swelling behavior were reported. Cytotoxicity test demonstrated that synthesized gels owned satisfactory cytocompatibility and were convenient for the application as biomaterials. Finally, the weak bovine serum albumin (BSA) adsorption on semi-IPNs by introducing METAC and controlling the content of PEG in gels demonstrated that they were of good protein resistance effect in biomedical applications. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41537.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
CHIRALITY
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2021
·Vol. 33
·期 7
·385-396
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IF 2.437
Qiuna Lu,Lei Chen,Qi Meng,Yan Jiang,Licheng Xie
A composite chiral interface (BSA-MB-MWCNTs) was prepared from bovine serum albumin (BSA), methylene blue (MB), and multi-walled carbon nanotubes (MWCNTs) for chiral recognition of amine enantiomers (1 S , 2 S )- N , N ′-dimethyl-1,2-cyclohexanediamine and (1 R , 2 R )- N , N ′-dimethyl-1,2-cyclohexanediamine. The BSA-based composite was characterized by field emission scanning electron microscopy (FESEM) and ultraviolet–visible spectroscopy (UV–Vis). The electrochemical responses towards the two enantiomers were analyzed via cyclic voltammetry (CV), electrochemical AC impedance method (EIS), and differential-pulse voltammetry (DPV). The experimental results showed that the combination of MWCNTs and BSA could effectively improve the overall identification efficiency, and the peak current displayed by the S -enantiomer is larger, indicating that the prepared chiral surface has stronger interaction with the R -enantiomer. Under optimized condition, the current value of the oxidation peak of the chiral modified electrode showed a good linear relationship towards the amine concentration in the range of 5.0 × 10 −3 to 5.0 × 10 −5 mmol·L −1 . The proposed electrochemical chiral interface is easy to handle and provides a promising electrochemical sensing platform that can be used to identify chiral amine enantiomers.
研究领域:
生物化学
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电化学传感
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材料科学
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DOI
高影响因子
EUROPEAN POLYMER JOURNAL
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2023
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IF 6
Jiaping Feng,Dan Huang,Jiaxin Hu,Jiulong Li,Juan Zhang,Hongquan Fu,Jinming Chang,Hejun Gao,Yunwen Liao
Due to the low adsorption capacity, poor adsorption selectivity and poor biocompatibility of bilirubin adsorption materials currently used, it is still a challenge to design an efficient bilirubin removal material for the treatment of hyperbilirubinemia. In this work, BSA-grafted (bovine serum albumin) porous amino polypyrrole was constructed by grafting bovine serum albumin onto porous amino polypyrrole composites. Comparing with porous amino polypyrrole, its removal rate was as high as 90% in 25 mg/L conjugated bilirubin solution, and it has significantly improved the selectivity of adsorption. In addition, it has good blood compatibility and biocompatibility. Superhydrophobic interaction, hydrogen bond and pore filling were the main reasons for bilirubin adsorption. BSA-grafted porous amino polypyrrole has potential application value in whole blood perfusion.
研究领域:
生物材料学
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医学工程
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生物化学
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高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 21
·3099-3104
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IF 1.9
Dong Li,Minghui Hua,Kun Fang,Rong Liang
High-performance magnetic resonance (MR) imaging contrast agents are the key to reliable and accurate diagnosis of diseases using the MR imaging technique. In particular, dual-modal MR imaging nanoprobes that integrated the advantages of a high contrast effect and impressive sensitivity of T1- and T2-weighted MR imaging, respectively, have attracted increasing attention in recent years. However, the current methods for the fabrication of the dual-modal MR imaging nanoprobes suffer from a tedious synthesis procedure, harsh synthesis condition and ambiguous toxicity, strongly hindering their clinic transformation. Herein, we proposed a biomineralization approach for the fabrication of bovine serum albumin (BSA)–Fe3O4 nanoparticles with undoubted biosafety and robust T1 and T2 MR imaging ability in a facile way under mild conditions. BSA not only served as a template to control the growth of nanocrystal, but also enabled the favorable water solubility of the nanoprobe. The outstanding biocompatibility of BSA–Fe3O4 nanoparticles was proven by systematic in vitro and in vivo toxicity assessments, and dual-modal T1 and T2 MR imaging in vivo was achieved successfully. In addition, the nanoprobe was successfully applied for in vivo MR imaging of liver with a broad time window, and the metabolic investigation of BSA–Fe3O4 nanoparticles revealed that they could be eliminated from the body, ensuring a negligible adverse effect. The proposed biomineralization approach opens a new way for the fabrication of dual-modal MR contrast imaging nanoprobe with undoubted biosafety, and other functional proteins also exhibit great potential for the fabrication of dual-modal MR contrast imaging nanoprobe with specific functions with respect to biomineralization.
研究领域:
医学影像学
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材料科学
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生物医学工程
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DOI
高影响因子
MOLECULES
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2023
·Vol. 28
·期 2
·880
·
IF 4.6
Huanhuan Guo,Zongguang Tai,Fang Liu,Jing Tian,Nan Ding,Zhongjian Chen,Shen Gao
Over the past decade, the dose of nanoparticles given to solid tumors has remained at a median of 0.7% of the injected dose. Most nanoparticles are trapped in a mononuclear phagocyte system (MPS), of which 85% are Kupffer cells. In our study, threshold doses of bovine serum albumin (BSA) nanoparticles were investigated for the uptake of Kupffer cells in vitro and in vivo. The antitumor effect and safety of albumin-bound paclitaxel (ABP) were improved by using threshold doses of BSA nanoparticles. We found a threshold dose of 20,000 nanoparticles per macrophage uptake in vitro and a saturation dose of 0.3 trillion nanoparticles in tumor-bearing mice. In vivo efficacy and safety evaluations demonstrated that the threshold doses of blank BSA nanoparticles could significantly improve the efficacy and safety of ABP against tumors compared with ABP alone. In this study, the delivery efficiency of ABP was improved by using blank nanoparticles to saturate Kupffer cells, which provided a new approach to studying the Kupffer cell saturation threshold and thus a new scheme for improving the curative effect of ABP.Keywords:nanoparticles;drug delivery;breast cancer;Kupffer cell;albumin-bound paclitaxel (ABP);bovine serum albumin (BSA)
研究领域:
药理学
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肿瘤学
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纳米技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Xin Li,Bing Li,Jie Huang,Haiyan Zhu,Ying Li,Gang Shi
Polydopamine (PDA)-based molecular imprinting photoelectrochemical (MIP-PEC) sensor is usually applied to recognize proteins. However, the abundant active functional groups on the PDA surface lead to a non-specific adsorption at the non-imprinted sites, resulting in a decrease in the ability of the MIP-PEC sensor to qualitatively analyze proteins. To solve this problem, in the present work, the polymer brush is specially designed to been grafted onto the PDA imprinted layer of the Si-based photoelectrode and then reduces the probability of non-specific adsorption, improving the specific detection ability. In addition, the Si-based photoelectrode was formed by successively modifying buried junction, nitrogen-doped carbon and TiO 2 on the Si wafer with micropyramid structure, enhancing the photocurrent density of the photoelectrode and improving the detection sensitivity. The experimental results suggest that the specific recognition ability of the MIP-PEC sensor to BSA was significantly improved with an increase of imprinting factor from 5.10 to 7.06. Also, the sensing signals showed a good linearity with BSA concentration in a range of 10 -16 -10 -9 mg·mL −1 , and a limit of detection of 2.203 × 10 -17 mg·mL −1 (S/N = 3). Furthermore, it was also successful to analysis as the template of acetylcholine was used, confirming the universality of this method.
研究领域:
生物传感技术
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材料科学
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分析化学
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Shuxian Hou,Ye Hong,Jihua Shang,Yimei Wang,Xuechao Shi,Xinxin Liu,Guoqiang Yang,Yuxuan Wang,Fei Ge,Yao Xiao,Chaldi Kaoutar,Yuan Wu,Jun Wang
The misuse of antibiotics has led to the growing problem of multidrug-resistant (MDR) bacteria, and there is still a lack of effective antibacterial agents that can replace antibiotics. Therefore, the design and development of multifunctional nanomaterials with long-term inhibitory effects on drug-resistant bacteria are extremely challenging. In this study, a multifunctional biomimetic self-assembly system, BSA-ZnO&Quercetin, based on bovine serum albumin (BSA), ZnO, and quercetin, was established using a simple and controllable method. The prepared self-assembly system has high stability and biocompatibility, and could fully combine the performance advantages of each component. BSA-ZnO&Quercetin showed excellent broad-spectrum antibacterial activity without inducing bacterial resistance. The related antibacterial mechanism of BSA-ZnO&Quercetin primarily involves biofilm inhibition and destruction, and inducing the production of reactive oxygen species, resulting in the death of the bacteria. The biomimetic self-assembly system BSA-ZnO&Quercetin constructed in this research is expected to replace antibiotics for antibacterial application.
研究领域:
纳米材料
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微生物学
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药物开发
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 186
·期 12
·1-10
·
IF 5.479
Li Xingmei,Ding Yanjun,Ling Jiang,Yao Wenyan,Zha Lagabaiyla,Li Na,Chang Yunfeng,Wang Yong,Cai Jifeng
Forensic saliva identification represents an increasingly useful auxiliary means of crime investigations, particularly in sex crimes. Salivary bacteria detection techniques have been shown to be viable methods for identifying the presence of saliva. A one-pot method is described for the fabrication of bovine serum albumin-stabilized SiC nanoparticles (SiC@BSA NPs). The SiC@BSA NPs were conjugated to antibacterial peptide GH12 to allow for fluorometric detection and imaging of bacteria in saliva. More specifically, the nanoprobe, with fluorescence excitation/emission maxima at 320/410 nm, was used to detect the oral bacteria S. salivarius levels. The detection limit is 25 cfu·mL −1 , and the assay can be performed within 40 min. The nanoprobe was also used to detect bacteria in forensic body fluids including blood, urine, and semen. In all cases, positive results were obtained with (mixed) samples containing saliva, while other saliva samples without saliva showed negative results. Fluorescent images of S. salivarius cells were obtained by implementing a high-content image analysis system. These results suggest that this new nanoprobe can be applied to screen for forensic saliva stains.
Schematic representation of the preparation of SiC@BSA-GH12 nanoprobe for fluorometric detection and imaging of S. salivarius in saliva.
研究领域:
生物技术
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法医学
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微生物学
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DOI
PubMed
高影响因子
RSC Advances
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2021
·Vol. 11
·期 25
·15323-15331
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IF 3.361
Yao Xu,Xiang Wang,Kang Song,Jun Du,Jinliang Liu,Yuqing Miao,Yuhao Li
Photodynamic therapy is a promising treatment method. The development of suitable photosensitizers can improve therapeutic efficacy. Herein, we report three iridium complexes (Ir1, Ir2, and Ir3), and encapsulate them within bovine serum albumin (BSA) to form nano-photosensitizers (Ir1@BSA, Ir2@BSA, and Ir3@BSA) for photodynamic therapy (PDT) of tumor cells. In the structures of Ir(III) complexes, we use the pyrazine heterocycle as part of the C^N ligands and explore the effect of different ligands on the ability to generate singlet oxygen (1O2) by changing the conjugation length of the ligand and increasing the coplanarity of the ligand. Besides, the fabricated nano-photosensitizers are beneficial to improve water dispersibility and increase cellular uptake ability. Through studying photophysical properties, 1O2 generation capacity, and cellular uptake performance, the results show that Ir1@BSA has the best photodynamic therapeutic effect on 4T1 tumor cells. This study provides an effective research basis for the further design of new nano-photosensitizers.
研究领域:
生物医学
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材料科学
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肿瘤学
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DOI
PubMed
高影响因子
Micro & Nano Letters
·
2019
·Vol. 14
·期 9
·952-956
·
IF 0.975
Yining Zhu,Wang Li,Cheng Ju,Xiang Gong,Wenhao Song,Yu Zhao,Ruijun Li
A bovine serum albumin-stabilised gold nanoclusters/Cu 2+ (BSA-Au NCs/Cu 2+ ) system was constructed as a fluorescent sensor for the ‘off–on’ detection of glutathione (GSH). The fluorescence of BSA-Au NCs could be effectively quenched by Cu 2+ due to the strong affinity between BSA-Au NCs and Cu 2+ . Then, coordination of Cu 2+ to GSH enabled the recovery of the fluorescence of BSA-Au NCs. To assess the selectivity of the BSA-Au NCs/Cu 2+ for GSH, K + , Mg 2+ , Ca 2+ , glucose, cysteine, tryptophan, and phenylalanine were examined under identical conditions, and all of them did not induce noticeable responses to BSA-Au NCs/Cu 2+ except for Cys. There was a good linear dependence of fluorescence intensity on the concentration of GSH in the range of 0.4–20.0 μM with the detection limit of 0.36 μM. The proposed sensor showed excellent performance in the selective detection of GSH.
研究领域:
分析化学
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纳米技术
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生物传感器
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DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2022
·Vol. 33
·期 24
·19331-19343
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IF 2.779
Li Weiyin,Zhao Tongtong,Ding Lingling
Au@BSA was prepared at pH 8, pH 9, pH 10, pH 11, pH 11.4, and pH 12 as the working electrode of the non-enzymatic glucose sensor by biological template method. The six kinds of gold-cluster film working electrodes sintered by Au@BSA show golden color, especially the golden films corresponding to pH 8 and pH 12 are obvious. The gold-cluster films at pH 8, pH 9, pH 10, and pH 11 showed mono-layer gold nanoparticles, while the gold-cluster film at pH 11.4 showed porous structure. The gold-cluster film prepared at pH 12 presents a multi-layer 3D structure composed of a large number of gold nanoparticles. The linear detection range of the non-enzymatic glucose sensor prepared at pH 8 is the widest among the six sensors, and its sensitivity is also better than the other four sensors except the sensor prepared at pH 12. The sensor with the gold-cluster film-modified working electrode prepared at pH 12 show the highest sensitivity (330.002 μA mM −1 cm −2 ), because multi-layer 3D structure can bring more electric catalytic active site for this sensor. The electrochemical impedance spectroscopy showed that the specific surface area of the sensor prepared at pH 12 was much larger than that of the other five sensors. We provide a pH cycling method for once shaping preparation that can be extended to other metal films or metal-oxide films electrochemical interfaces. The Au@BSA non-enzymatic glucose sensor prepared in this paper is stable and can resist the toxicity of excessive chloride ions without losing activity.
研究领域:
生物传感器
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电化学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2017
·
IF 2.625
Shuang Zhao,Zhengping Li,Yunxiao Li,Jun Yu,Guohua Liu,Ruping Liu,Zhao Yue
Herein we present the development of a bovine serum albumin (BSA)-coated fluorescent gold nanocluster (BSA-AuNC)/graphene oxide (GO) hybrid nanocomposite-based photoelectrochemical (PEC) biosensor, which can be used for multichannel detection of both hydrogen peroxide (H 2 O 2 ) and dopamine (DA) within one chip at negative and positive potentials, respectively. BSA-AuNC-based biosensors generally exhibit poor PEC sensing performance owing to their poor separation of photoexcited electron-hole pairs and low electrode coverage. Consequently, improved AuNC/GO hybrid nanocomposites and a layer-by-layer (LBL) method are introduced in this study. The enhancement was investigated by exploring the optimum concentration of GO (0.12 mg mL −1 ) and LBL number (four layers) for the preparation of the BSA-AuNC materials, revealing that GO plays different enhancement roles in the detection of H 2 O 2 and DA. Compared to the results from other gold nanoclusters with different ligands, the photoelectrical enhancement of BSA-AuNCs can be achieved for both negative and positive potentials simultaneously with the same optimum GO concentration and LBL number. Thus, the detection of H 2 O 2 and DA was achieved by BSA-AuNC/GO multilayers with enhanced sensing properties and easier fabrication processes. The two enhancement methods acting together led to an improvement in the limits of detection (LODs) from 325 μM to 23 μM (H 2 O 2 ) and from 7.45 μM to 1.5 μM (DA). Thus, our strategy shows great potential for the improvement of PEC sensing structure, and provides a method for multichannel detection.
研究领域:
生物传感
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纳米材料
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）