BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Yaru Zhang,Jing Ren,Quan Wang,Shuxiang Wang,Shuna Li,Hongya Li
In the current work, studies were conducted for understanding oxidation characteristics and biodegradation potential of a dye-decolorizing peroxidases from Bacillus amyloliquefaciens (BaDyP) for triphenylmethane dye crystal violet (CV). Recombinant BaDyP from Bacillus amyloliquefaciens MN-13 exhibits a broad substrates profile. It can oxidize ABTS, veratryl alcohol, Mn 2+ , azo dye reactive black 5, anthraquinone dye reactive blue 19, but cannot oxidize guaiacol. The maximum BaDyP activity against ABTS was obtained at 30 ℃ and pH 4.0. When CV was treated by BaDyP for 72 h, the degradation percentage of 63.0 % was observed. UV–vis spectra and HPLC/MS analysis indicated that CV degradation occurred via N -demethylation and cleavage of conjugated chromophore structure. The identified degradation products included stepwise N -demethylated intermediates during the N -demethylation of CV and, 4-( N -methylamino)-4′-( N ’-methylamino) benzophenone and 4-( N -methylamino)-4′-amino benzophenone after cleavage of conjugated chromophore structure. Based on these data, a hypothetic mechanism of CV degradation mediated by BaDyP was proposed in this study. Finally, it is worth to be noted that BaDyP possessed high decolorization efficiency (72.3 % removal ADMI) for actual dye effluent. These results demonstrated that BaDyP might be used as an efficient biocatalyst for the enzymatic pretreatment of dyes in wastewater.
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BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2020
·Vol. 44
·期 4
·727-735
·
IF 2.419
Wang Hongrao,Han Shuran,Wang Jiayi,Yu Shuyu,Li Xiaoyan,Lu Lei
Laccases are versatile oxidases that are capable of decolorizing various synthetic dyes. Recombinant Bacillus amyloliquefaciens laccase was immobilized as magnetic cross-linked enzyme aggregates (M-CLEAs) for application in dye decolorization. Several parameters influencing the activity recovery were evaluated during the synthesis of M-CLEAs. With ammonium sulfate as precipitant, maximum activity was recovered by cross-linking with 0.16% glutaraldehyde for 1 h. The prepared M-CLEAs exhibited improved activity under alkaline conditions. It remained 74% activity after incubation at 60 °C for 5 h. Enhanced tolerance towards NaCl was also observed for the M-CLEAs, with 68% activity remaining in the presence of 1 M NaCl. The immobilized laccase could rapidly decolorize more than 93% of reactive black 5 and indigo carmine in 1 h, while its catalytic efficiency towards reactive blue 19 was relatively low. After four cycles of consecutive reuse, the M-CLEAs could decolorize 92% of indigo carmine. The easy recovery and reusability of M-CLEAs facilitate the potential application of bacterial laccase in dye decolorization.
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Systems Microbiology and Biomanufacturing
·
2023
·
IF 0
Li Chang-Long,Li Meng,Zhang Wei-Guo,Xu Jian-Zhong
Menaquinone-7 (MK-7) has an important role in preventing diseases such as cardiovascular disease and osteoporosis. In this study, a combination strategy of strain improvement and medium optimization is investigated to increase MK-7 production in Bacillus amyloliquefaciens . Conventional breeding method was first used to modify the biosynthetic pathway to construct a MK-7 high-producing strain by atmospheric and room temperature plasma mutagenesis and protoplast fusion. The resulted strain Ba-4 with resistance to sulfaguanidine, 1-hydroxy-2-naphthoic acid, menadione, 2-deoxy- d -glucose and rifampicin as well as sensitive to β-fluoropyruvate produced 73.57 ± 1.61 mg/L of MK-7, which was 1.36 times more than that of the parent strain H.β.D.R.-5 (i.e., 31.12 ± 1.40 mg/L). Subsequently, single-factor optimization and response surface methodology (RSM) were used to optimize the medium components for increasing MK-7 production by strain Ba-4. Strain Ba-4 produced 90.43 ± 1.32 mg/L of MK-7 under the single-factor optimized medium. Moreover, the results of response surface methodology indicated that glycerol, soy peptone and Tween-80 had significant effects on MK-7 production, and the highest MK-7 production (i.e., 95.03 ± 1.01 mg/L) was obtained under the optimized medium, which was 0.29 times higher than that of the initial medium. These results confirmed that the conventional breeding methods and fermenter control system are effective strategies in improving MK-7 production by B. amyloliquefaciens .
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Xiaozhou Xia,Yu Fu,Liang Ma,Hankun Zhu,Yong Yu,Hongjie Dai,Jiadong Han,Xin Liu,Zhengfang Liu,Yuhao Zhang
Long-term high salt intake exerts a negative impact on human health. The excessive use of sodium substitutes in the food industry can lead to decreased sensory quality of food. γ-Glutamyl peptides with pronounced taste-enhancing effects can offer an alternative approach to salt reduction. However, the content and yield of γ-glutamyl peptides in natural foods are relatively low. Enzyme-catalyzed synthesis of γ-glutamyl peptides provides a feasible solution. In this study, Pleurotus geesteranus was hydrolyzed by Flavourzyme to generate protein hydrolysates. Subsequently, they were modified by Bacillus amyloliquefaciens γ-glutamyl transpeptidase to generate γ-glutamyl peptides. The reaction conditions were optimized and their taste-enhancing effects were evaluated. Their peptide sequences were identified by parallel reaction monitoring with liquid chromatography–tandem mass spectrometry and analyzed using molecular docking. The optimal conditions for generation of γ-glutamyl peptides were a pH of 10.0, an enzyme condition of 1.2 U/g, and a reaction time of 2 h, which can elicit a strong kokumi taste. Notably, it exhibited a remarkable taste-enhancing effect for umami intensity (76.07%) and saltiness intensity (1.23-fold). Several novel γ-glutamyl peptide sequences were found by liquid chromatography–tandem mass spectrometry, whereas the binding to the calcium-sensing receptor was confirmed by molecular docking analysis. Overall, γ-glutamyl peptides from P. geesteranus could significantly enhance the umami and salt tastes, which can serve as promising taste enhancers.
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Journal of Fungi
·
2026
·Vol. 12
·期 8
·576
·
IF 4.3
Dan-Na Ma,Yihan Niu,Mengli Chen,Jun-Nan Yao,Huiming Wu,Taiying Li
Fusarium wilt, caused by the soil-borne fungusFusarium oxysporumf. sp.lycopersici(Fol), is a devastating disease that severely constrains tomato production worldwide. In this study, an endophytic bacterium, designated ZN-S18, was isolated from healthy tomato stems and identified asBacillus amyloliquefaciensbased on phylogenetic analyses of the 16S rRNA andrpoBgenes. Dual culture assays demonstrated that ZN-S18 exhibited strong antagonistic activity against Fol and significantly inhibited fungal mycelial growth. The cell-free culture filtrate of ZN-S18 markedly suppressed Fol spore germination and mycelial growth, and the inhibitory effect on spore germination was irreversible. LC-MS analyses confirmed that ZN-S18 produces the cyclic lipopeptides surfactin and iturin A. In planta assays further demonstrated that pretreatment with ZN-S18, its culture filtrate, or the combination of surfactin and iturin A significantly reduced Fusarium wilt in tomato seedlings. Moreover, the culture filtrate disrupted fungal cell wall integrity, as indicated by the increased sensitivity of Fol to Congo red stress. Notably, the combined application of surfactin and iturin A exhibited significantly stronger antifungal activity than either compound alone under Congo red-containing conditions, suggesting a synergistic effect on fungal cell wall integrity. Collectively, these findings indicate that the endophytic strainB. amyloliquefaciensZN-S18 is a promising biocontrol agent against tomato Fusarium wilt, primarily through the synergistic action of surfactin and iturin A. This study provides an environmentally friendly alternative to chemical fungicides and establishes a foundation for future investigations into the molecular mechanisms underlying lipopeptide-mediated antagonism.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.5
Linru Huang,Fengping Liao,Kuntai Li,Yinyin Cao,Minghui Tan
The development of multifunctional and biodegradable composite films represents a promising approach to enhancing food preservation. Herein, a chitosan-based composite film was designed by incorporating bacterial cellulose (as a mechanical reinforcement agent) and Bacillus amyloliquefaciens HY2–1 lipopeptides (as an antimicrobial component), with the aim of enabling its potential application in food packaging. A systematic investigation was conducted to evaluate the influence of varying HY2–1 lipopeptides concentrations on the key performance metrics of the composite films, including mechanical strength, barrier properties, antioxidant capacity, antimicrobial activity, and practical efficacy in fruit preservation. The results revealed a concentration-dependent enhancement in functional properties of the composite films with increasing of HY2–1 lipopeptides incorporation. At the optimal HY2–1 lipopeptides concentration of 2.4 mg/mL, the composite film demonstrated significantly improved antioxidant activity, with DPPH radical scavenging of 48.21% (8-fold that of the control) and ABTS radical scavenging of 60.73% (3-fold that of the control). Besides, the composite film exhibited significantly enhanced antimicrobial activities against a broad spectrum of pathogens, including Gram-positive bacteria ( Listeria monocytogenes and Staphylococcus aureus ), Gram-negative bacteria ( Escherichia coli ), and the fungal Penicillium digitatum , compared to the control film. Furthermore, the lipopeptides-incorporated composite film effectively preserved critical shelf-life indicators of citrus and strawberry under ambient storage conditions, including color retention, firmness maintenance, and nutritional content preservation, thereby demonstrating excellent practical preservation efficacy. Overall, this study designs a high-performance, fully bio-based composite film by integrating multifunctional lipopeptides into a chitosan matrix, presenting a potent and natural strategy to extend the shelf-life of perishable foods.
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BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Xin-Yue Chen,Hui-Zhong Sun,Bin Qiao,Chang-Hao Miao,Zheng-Jie Hou,Shu-Jing Xu,Qiu-Man Xu,Jing-Sheng Cheng
The application of antibacterial lipopeptides is limited by high cost and low yield. Herein, the exogenous L-proline significantly improved lipopeptide production by Bacillus amyloliquefaciens HM618. A recombinant Corynebacterium glutamicum producing high levels of proline using genetically modifying proB and putA was used to establish consortium, to improve lipopeptide production of strain HM618. Compared to a pure culture , the levels of iturin A, fengycin, and surfactin in consortium reached 67.75, 39.32, and 37.25 mg L -1 , respectively, an increase of 3.19-, 2.05-, and 1.63-fold over that produced by co-cultures of B. amyloliquefaciens and recombinant C. glutamicum with normal medium. Commercial amylase and recombinant Pichia pastoris with a heterologous amylase gene were used to hydrolyze kitchen waste. A three-strain consortium with recombinant P. pastoris and C. glutamicum increased the lipopeptide production of strain HM618 in medium containing KW. This work provides new strategies to improve lipopeptide production by B. amyloliquefaciens.
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Scientific Reports
·
2016
·Vol. 6
·期 1
·1-13
·
IF 5.228
Raza Waseem,Ling Ning,Yang Liudong,Huang Qiwei,Shen Qirong
It is important to study the response of plant pathogens to the antibiosis traits of biocontrol microbes to design the efficient biocontrol strategies. In this study, we evaluated the role of volatile organic compounds (VOCs) produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum (RS). The VOCs of SQR-9 significantly inhibited the growth of RS on agar medium and in soil. In addition, the VOCs significantly inhibited the motility traits, production of antioxidant enzymes and exopolysaccharides, biofilm formation and tomato root colonization by RS. The strain SQR-9 produced 22 VOCs, but only nine VOCs showed 1–11% antibacterial activity against RS in their corresponding amounts; however, the consortium of all VOCs showed 70% growth inhibition of RS. The proteomics analysis showed that the VOCs of SQR-9 downregulated RS proteins related to the antioxidant activity, virulence, carbohydrate and amino acid metabolism, protein folding and translation, while the proteins involved in the ABC transporter system, amino acid synthesis, detoxification of aldehydes and ketones, methylation, protein translation and folding, and energy transfer were upregulated. This study describes the significance and effectiveness of VOCs produced by a biocontrol strain against tomato wilt pathogen.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Hongxuan Li,Yubo Zhang,Yaping Zheng,Xuejian Li,Zimu Li,Chaoxin Man,Yu Zhang,Yujun Jiang
The probiotic strain Bacillus amyloliquefaciens JM033 ( B. amyloliquefaciens JM033), isolated from the traditional Chinese fermented food Sufu (also known as Fu-ru or fermented bean curd), is distinguished by its high production of exopolysaccharides (EPS). The EPS (BAP-1) produced by this strain was purified and its structure analyzed. BAP-1 is a novel hybrid fructan with a molecular weight of 17.6 kDa. It is composed of →6)- β -D-Fru f -(2 → and →1,6)- β -D-Fru f -(2→, which form the backbone, with a branched chain of β -D-Fru f -(2 → attached at the 1-position of residue B. In vivo studies on mice indicated that BAP-1 improves immunity in immunosuppressed mice by enhancing humoral immunity ( P < 0.01), monocyte-macrophage phagocytosis ( P < 0.01), and NK cell killing activity ( P < 0.05). Additionally, BAP-1 was found to improve the composition of the intestinal microbiota and stimulate the production of short-chain fatty acids. Notably, BAP-1 exhibited a significant effect on the proliferation of Akkermansia . Therefore, BAP-1 shows promise as a prebiotic and may contribute to the development of new immunomodulatory agents.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Xiaodi Wang,Qiaolei Zhu,Yuhan Yan,Yao Yu,Wanting Yue,Yunping Zhu,Zhou Chen
This study aimed to identify novel antioxidant peptides extracted from okara fermentation broth, and to investigate the molecular mechanisms using in silico and in vitro approaches. The results demonstrated that three novel peptides—CKLLL, FMAV and SVCC—were identified, among which CKLLL exhibited the strongest DPPH radical scavenging activity (IC 50 = 30.21 ± 4.27 μM). Simulated gastrointestinal digestion showed CKLLL had stable DPPH scavenging activity and improved ABTS and hydroxyl radical scavenging capacities. Furthermore, all three antioxidant peptides effectively protected HepG2 cells against H 2 O 2 -induced damage, with CKLLL>SVCC>FMAV ranking highest to lowest in terms of protective effect. Peptide and Keap1 protein docking results indicate that the -CDOCKER energy of CKLLL (89.13 kcal/mol) is higher than that of FMAV and SVCC (55.35 and 48.01 kcal/mol). These findings suggest that these three peptides, especially CKLLL, have the potential to serve as antioxidant peptides, and provide a more reliable screening method for developing natural plant-derived antioxidant peptides.
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Journal of Functional Foods
·
2026
·
IF 3.9
Xiqian Tan,Zhiqi Xing,Bolin Zhou,Lijun You,Fangchao Cui,Xuepeng Li,Jianrong Li
Hyperuricemia (HUA) is a metabolic disorder that can trigger gout and cause renal dysfunction. In this study, microbial fermentation with Bacillus amyloliquefaciens Y11 was employed to ferment salmon dark meat. Subsequently, novel peptides with high xanthine oxidase (XOD) inhibitory activity were derived from the fermented samples, purified, and identified. The hypouricemic effect of these peptides was evaluated using an in vitro HK-2 cell HUA model. Five fractions (G1–G5) were isolated via ultrafiltration and Sephadex G15 gel chromatography, and the fractions with higher XOD inhibition rates (G1 and G5) were combined to prepare a mixed fraction. Subsequently, 4122 peptides were identified from the mixed fraction using liquid chromatography with tandem mass spectrometry. Based on predictive bioactivity, toxicity, allergenicity, and solubility scores, five peptides and seven corresponding truncated peptides were selected for synthesis. Their XOD inhibitory activity was assessed, and molecular docking analysis was performed. Ultimately, GEADFMDYGR and its truncated peptide GEADF, which showed high XOD inhibitory activity in vitro (inhibition rates of 90.72 ± 2.85% and 94.06 ± 1.30%, respectively), were screened out. In the HK-2 HUA model, both peptides significantly reduced intracellular uric acid levels. Specifically, 0.5 mg/mL GEADFMDYGR and GEADF decreased the uric acid levels in these cells by 39.70 ± 8.75 and 27.43 ± 3.31 μmol/L, respectively. Further analyses revealed that GEADFMDYGR and GEADF are reversible mixed-type XOD inhibitors. These findings provide new insights and experimental evidence guiding the development of novel therapeutic peptides for HUA.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2016
·Vol. 100
·期 17
·7639-7650
·
IF 3.376
Raza Waseem,Wang Jichen,Wu Yuncheng,Ling Ning,Wei Zhong,Huang Qiwei,Shen Qirong
The production of volatile organic compounds (VOCs) by microbes is an important characteristic for their selection as biocontrol agents against plant pathogens. In this study, we identified the VOCs produced by the biocontrol strain Bacillus amyloliquefaciens T-5 and evaluated their impact on the growth and virulence traits of tomato bacterial wilt pathogen Ralstonia solanacearum. The results showed that the VOCs of strain T-5 significantly inhibited the growth of R. solanacearum in agar medium and in soil. In addition, VOCs significantly inhibited the motility traits, root colonization, biofilm formation, and production of antioxidant enzymes and exopolysaccharides by R. solanacearum . However, no effect of VOCs on the production of hydrolytic enzymes by R. solanacearum was observed. The strain T-5 produced VOCs, including benzenes, ketones, aldehydes, alkanes, acids, and one furan and naphthalene compound; among those, 13 VOCs showed 1–10 % antibacterial activity against R. solanacearum in their produced amounts by T-5; however, the consortium of all VOCs produced on agar medium, in sterilized soil, and in natural soil showed 75, 62, and 85 % growth inhibition of R. solanacearum , respectively. The real-time PCR analysis further confirmed the results when the expression of different virulence- and metabolism-related genes in R. solanacearum cells was decreased after exposure to the VOCs of strain T-5. The results of this study clearly revealed the significance of VOCs in the control of plant pathogens. This information would help to better comprehend the microbial interactions mediated by VOCs in nature and to develop safer strategies to control plant disease.
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