PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2024
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IF 4.2
Shan Ye,Siyu Zhou,Yihang Ma,Jiahao Yang,Xuqi Shi,Ruoyu Zhang,Zhuhong Yang,Deliang Peng,Zhong Ding
Root-knot nematodes ( Meloidogyne spp.) are highly destructive pests that cause significant yield losses annually. Biological control of nematodes has emerged as a potential alternative in sustainable agriculture. In this study, we originally isolated Bacillus cereus G5 from the rhizosphere soil of rice ( Oryza sativa ). Treatment with the fermentation supernatant of G5 in vitro demonstrated high toxicity to second-stage juveniles (J2) of Meloidogyne graminicola and remarkably inhibited egg hatching. Moreover, G5 steadily colonized rhizosphere soil and rice seedlings, and exhibited excellent biocontrol efficacy against M. graminicola under greenhouse conditions . Notably, the volatile organic compounds (VOCs) produced by G5 displayed high fumigant activity against M. graminicola. The G5 VOCs efficiently reduced the gall index and nematode population in rice roots, while also promoting rice growth in double-layered pot tests. Additionally, the expression of defense genes involved in the salicylic acid ( OsNPR1 , OsWRKY45, OsPAL1 ), jasmonic acid ( OsJaMYB , OsAOS2 ) and ethylene ( OsACS1 ) signalling pathways was significantly upregulated in rice seedlings treated with G5 VOCs. This suggests that G5 VOCs contribute to eliciting plant defense responses. Furthermore, we identified 14 major VOCs produced by G5 using solid-phase micro-extraction gas chromatography and mass spectrometry (SPEM–GC–MS). Notably, allomatrine, morantel, 1-octen-3-ol and 3-methyl-2-butanol displayed strong contact nematicidal activity. Among these, only 1-octen-3-ol demonstrated fumigant activity against J2s of M. graminicola, with an LC 50 value of 758.95 mg/L at 24 h. Overall, these results indicated that the B. cereus G5 and its synthetic VOCs possess high potential as biocontrol agents for managing root-knot nematodes .
研究领域:
植物病理学
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农业科学
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分子生物学
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DOI
PubMed
高影响因子
Toxins
·
2023
·Vol. 15
·期 1
·65
·
IF 4.2
Guoli Xue,Yanjun Qu,Dan Wu,Shuyuan Huang,Yuqing Che,Jing Yu,Ping Song
Aflatoxin is a potent mycotoxin and a common source of grain contamination that leads to great economic losses and health problems. Although distilled baijiu cannot be contaminated by aflatoxin, its presence in the brewing process affects the physiological activities of micro-organisms and reduces product quality.Bacillus cereusXSWW9 capable of degrading aflatoxin B1(AFB1) was isolated from daqu using coumarin as the sole carbon source. XSWW9 degraded 86.7% of 1 mg/L AFB1after incubation at 37 °C for 72 h and tolerated up to 1 mg/L AFB1with no inhibitory effects. Enzymes in the cell-free supernatant of XSSW9 played a significant role in AFB1degradation. The AFB1-degradation activity was sensitive to protease K and SDS treatment, which indicated that extracellular proteins were responsible for the degradation of AFB1. In order to investigate the AFB1-degradation ability of XSSW9 during the baijiu brewing process, AFB1and XSWW9 were added to grain fermentation (FG-T) and normal grain fermentation without AFB1, while normal grain fermentation without AFB1and XSWW9 was used as a control (FG-C). At the end of the fermentation, 99% AFB1was degraded in the residue of fermented grains. The differences of microbial communities in the fermented grains showed that there were no significant differences between FG-T and FG-C in the relative abundance of dominant genera. The analysis of volatile compounds of their distillation showed that the contents of skeleton flavor components was similar between FG-T and FG-C. These results offer a basis for the development of effective strategies to reduce the effect of AFB1on the brewing process and ensure that the production of baijiu is stable.Keywords:aflatoxin B1;biodegradation;Bacillus;baijiu;aroma compoundsKey Contribution:The bioaugmentation of the zaopei can further improve the safety factor during the fermentation of the grains without affecting the quality of baijiu.
研究领域:
食品科学
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微生物学
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毒理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2020
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IF 3.365
Chen Xujun,Li Shuyi,Cong Xin,Yu Tian,Zhu Zhenzhou,Barba Francisco J.,Marszalek Krystian,Puchalski Czesław,Cheng Shuiyuan
Selenium is an essential trace element and micronutrient for human health. Application of organic selenium in plants and microorganisms as trace element supplement is attracting more and more attention. In this study, Bacillus cereus, an important probiotic, was used for selenium enrichment with sodium selenite as selenium source. The growth curve of B. cereus was investigated, and 150 μg/ml was selected as the concentration of selenium for B. cereus fermentation. With application of response surface methodology, the optimal fermentation conditions were obtained as follows: inoculation quantity of 7%, culture temperature of 33°C, and shaking speed of 170 rpm, leading to the maximal selenium conversion ratio of 94.3 ± 0.2%. Field emission scanning electron microscope and energy dispersive spectrometry evidenced that inorganic selenium had been successfully transformed. This study may contribute to get a strain with high Se conversion ratio, so as to extract organic selenium in the form of selenoprotein to be used for further application.
研究领域:
微生物学
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营养科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Foods
·
2022
·Vol. 11
·期 22
·3722
·
IF 5.561
Hongbin Wang,Yuanfu Zhang,Zhaoting Yuan,Xiaotong Zou,Yuan Ji,Jiayi Hou,Jinfang Zhang,Fuping Lu,Yihan Liu
Until now,Streptoverticillium mobaraensetransglutaminase (TG) is the only commercialized TG, but limited information is known about its selection tendency on crosslinking sites at the protein level, restricting its application in the food industry. Here, four recombinantBacillusTGs were stable in a broad range of pH (5.0–9.0) and temperatures (<50 °C), exhibiting their maximum activity at 50–60 °C and pH 6.0–7.0. Among them, TG ofB. cereus(BCETG) demonstrated the maximal specific activity of 177 U/mg. A structural analysis indicated that the Ala147-Ala156 region in the substrate tunnel of BCETG played a vital role in catalytic activity. Furthermore, bovine serum albumin, as well as nearly all protein ingredients in soy protein isolate and whey protein, could be cross-linked by BCETG, and the internal crosslinking paths of three protein substrates were elucidated. This study demonstratedBacillusTGs are a candidate for protein crosslinking and provided their crosslinking mechanism at the protein level for applications in food processing.Keywords:transglutaminase;enzymatic characterization;structural analysis;crosslinking properties;protein substrate crosslinking mechanism
研究领域:
食品科学
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生物化学
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酶学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhichao Huang,Xiaoming Yuan,Zhenjun Zhu,Ying Feng,Na Li,Shubo Yu,Chun Li,Bo Chen,Shi Wu,Qihui Gu,Jumei Zhang,Juan Wang,Qingping Wu,Yu Ding
Bacillus cereus is a pathogenic bacterium that causes food contamination, resulting in food poisoning such as diarrhea and emesis. Therefore, it is crucial to develop effective strategies to control this bacterium. In this study, we isolated and characterized a novel B. cereus phage, named DZ1. Morphological and genomic analyses revealed that phage DZ1 is a new species belonging to the Andromedavirus genus. Phage DZ1 was tolerant to a wide range of pH values (5–9), temperatures (4–55 ℃), and high concentrations of NaCl solution (1000 mM). B. cereus with 21 different sequence types (STs) can be lysed by phage DZ1. Importantly, phage DZ1 inhibited B. cereus growth in spiked rice substrates or milk up to 36 and 72 h, respectively, with suppression of 3 log. Therefore, phage DZ1 is a useful biocontrol agent for the control of B. cereus in the food industry.
研究领域:
微生物学
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食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
·
IF 3.3
He Ying,Huang Ao,Liu Yun
Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, and their combinations—were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7–12, and IC 13–14). Among these, the CI 7–12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30–60 °C) and pH range (6–10), with a half-life of 128 days. Kinetic analysis revealed a V max of 20.04 μmol/h and a K m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.
研究领域:
微生物学
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生物化学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
ARCHIVES OF MICROBIOLOGY
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2022
·Vol. 204
·期 3
·1-12
·
IF 2.667
Ming Shanxiu,Chen Xujun,Zhang Na,Li Shuyi,Zhu Zhenzhou,Cheng Shuiyuan
Microorganisms can produce many antibiotics against bacteria and fungi, which have been used as a potential choice of new antibiotics. In this paper, we studied the characteristics of antibacterial substances by Bacillus cereus BC1. The results showed that the acid-precipitated substance played the main role in antibacterial activity, and further characterization indicated that the antibacterial substance might be a lipopeptide substance. Then the antibacterial spectrum suggested that the antibacterial substance had an inhibitory effect on Gram-positive bacteria and fungi, while selenium-riched antibacterial substance of Bacillus cereus BC1 could significantly enhance the inhibition. Then the morphological effects of the antibacterial substance to indicator bacteria were determined. The effects of different treatment methods on the stability of antibacterial substances were studied and the results showed that the antibacterial substance was stable to heat, ultrasonic, and ultraviolet treatment, and their antibacterial activity would not be greatly affected. However, they were sensitive to pepsin. The optimum pH range of antibacterial activity was 3–5. This study may contribute to reusing the fermentation supernatant often discarded in the previous fermentation process. At the same time, the lipopeptide antibacterial substance extracted from the fermentation broth of selenium-enriched Bacillus cereus BC1 can be used in the development of antibiotics and biopesticides, and open up a new way for the control of plant diseases.
研究领域:
微生物学
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生物技术
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药学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2022
·
IF 14.224
Sinan Zhang,Jihong Wang
Microbial degradation of chlortetracycline (CTC) is an effective bioremediation method. In the present study, an enrichment technique was used to isolate a Bacillus cereus LZ01 strain capable of effectively degrading CTC from cattle manure. Response surface methodology was used to identify optimized conditions under which strain LZ01 was able to achieve maximal CTC removal (83.58%): temperature of 35.77 °C, solution pH of 7.59, CTC concentration of 57.72 mg/L and microbial inoculum of 0.98%. The antibacterial effect of CTC degradation products on Escherichia coli was investigated by the disk diffusion test, revealing that the products by LZ01 degradation of CTC exhibited lower toxicity than parent compound. Shake flask batch experiments showed that the biodegradation of CTC was a synergistic effect of intracellular and extracellular enzymes, and intracellular enzyme had a better degradation effect on CTC (77.56%). Whole genome sequencing revealed that genes associated with ring-opening hydrolysis, demethylation, deamination and dehydrogenation in strain LZ01 may be involved in the biodegradation of CTC. Subsequent seven possible biodegradation products were identified by LC-MS analyses, and the biodegradation pathways were proposed. Overall, this study provides a theoretical foundation for the characterization and mechanism of CTC degradation in the environment by Bacillus cereus LZ01.
研究领域:
环境科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2021
·
IF 5.263
Sinan Zhang,Jihong Wang
Biochar-immobilized microorganism technology is an effective way to remove antibiotic contamination from water bodies. In this study, three common Chinese medicine residues (forsythia, erding and honeysuckle) were selected as raw materials for pyrolysis at different temperatures. The biochar obtained was used as a carrier to immobilize the highly efficient chlortetracycline-degrading bacterium Bacillus cereus LZ01 and analyze the complex’s removal characteristics and removal mechanism chlortetracycline. The results showed that the Chinese medicine residue biochar pyrolyzed at 500 °C had a higher removal capacity for chlortetracycline and could adsorb more microorganisms. The erding medicine residue biochar (EBC-500) pyrolyzed at 500 °C possessed a more developed pore structure and suitable nutrients. And the alkalinity of EBC-500 had the effect of relieving the inhibition of bacteria by acid, providing more suitable living conditions for the growth of bacteria, and improving the removal of chlortetracycline. Orthogonal experiments determined the optimal conditions for microbial immobilization by biochar: 10% microbial inoculum, 0.5 g biochar dosage, 0.097 - 0.15 mm biochar particle size, and 36 h immobilization time. The FT-IR spectra showed that the complex integrated all functional groups of biochar and degrading bacteria. The comparison of the removal effect before and after inactivation revealed that the removal mechanism of chlortetracycline in water by the complex was mainly determined by a combination of microbial degradation and biochar surface adsorption. This study provides a method for preparing an efficient biochar–microbial complex using Chinese medicine residue waste substrate, which provides a new idea for removing chlortetracycline from water.
研究领域:
环境科学
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微生物学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jiaxun Li,Keyuan Qin,Yuting Shang,Zhenjun Zhu,Yangfu Li,Junhui Wu,Moutong Chen,Liang Xue,Qingping Wu,Juan Wang,Yu Ding
Bacillus cereus is a significant cause of foodborne illnesses, particularly through starchy foods like rice and pasta, leading to the vomiting symptom. The primary toxin involved, cereulide, is preformed within contaminated food and exhibits high resistance to heat, making it particularly difficult to control. Since cereulide persists even after standard food processing methods, its prevention and control remain critical for the food industry. Isoleucine (Ile), a branched-chain amino acid (BCAA), has been linked to the regulation of cereulide production, but the exact mechanisms remain unclear. Based on untargeted metabolomics analysis using liquid chromatography-mass spectrometry (LC-MS), this study demonstrated that Ile supplementation markedly downregulated the folate biosynthesis pathway in B. cereus . Supplementation with folic acid partially restored cereulide production that had been suppressed by Ile. In addition, RT-qPCR analysis revealed significant changes in the expression of key enzymes involved in folate metabolism and related pathways following Ile exposure, further supporting the notion that Ile inhibits cereulide synthesis by disruption of folate metabolism. Collectively, these findings suggest a novel mechanism by which Ile regulates cereulide production via folate metabolism, providing new insights for the effective prevention and control of cereulide production in B. cereus .
研究领域:
食品微生物学
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代谢组学
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毒理学
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分子生物学
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微生物生理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Yingying Sun,Lele Shao,Yana Liu,Bo Zou,Han Wang,Xingmin Li,Ruitong Dai
Bacillus cereus spores pose a significant concern during food processing due to their high resistance to environmental stress. Ohmic heating (OH) is an emerging and alternative heating technology with potential for inactivating such spores. This study evaluated the inactivation effects and the biological property changes of Bacillus cereus spores during OH treatments. OH effectively inactivated spores in milk, orange juice, broth, rice soup, and buffer solution in less time than oil bath heating (OB). A decrease in NaCl content improved spore inactivation at the same temperature. Spores were more sensitive to acid at 80–85 °C with OH treatment. Furthermore, OH at 10 V/cm and 50 Hz could reduce the spore resistance and inhibit an increase in spore hydrophobicity and spore aggregation. Both heating methods resulted in significant dipicolinic acid (DPA) leakage and damage to the cortex and inner membranes of the spores. However, OH at 10 V/cm and 50 Hz had the lowest DPA leakage and inflicted the least damage to the inner membrane. The damage to the spore's inner membrane was considered the primary reason for inactivation by OB and OH treatments. Still, OH at 10 V/cm and 50 Hz might also block the germination or outgrowth of treated spores or cause damage to the spore core.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Surfaces and Interfaces
·
2021
·
IF 4.837
Zhongxuan Xu,Diandi Li,Yuping Tan,Qinyu Yang,Han Xiong,Jiguang Li,Hong Deng,Zhang Lin
Microbial treatment of heavy metals is a low-cost and environmentally friendly method. During the treatment of chromium pollution, environmental media has a great impact on the efficiency of bacteria Cr immobilization. However, when Cr(VI) coexist with different metal cations , the reduction of Cr(VI) and the immobilization of Cr by bacteria are still unclear. In our research, although bacteria were significantly promoted the reduction of Cr(VI) with the coexistence of Mn(II), the bacteria produced the maximum of soluble organic Cr(III). In the performance of immobilization Cr, Mg(II) group has the highest chromium immobilizing rate. In addition, Cd(II) has the strongest inhibitory effect on Cr(VI) reduction and Cr immobilization. The SEM results show that Cr were stored in the cells. However, XRD analysis did not demonstrate the crystal structure. Our research investigated the ability of bacteria to reduce and immobilize Cr with the coexistence of 9 different metal ions, and provided a reference for a deeper understanding of the relationship between bacteria and metal ions.
研究领域:
环境生物学
·
微生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）