CHEMICAL ENGINEERING SCIENCE
·
2025
·
IF 4.3
Chen Shi,Ya-Kun Wang,Long Yan,Yu-Hong Kang,Guang-Hui Liu,Yong Gao,Ya-Nan Zhu,Xian-Yong Wei,Zhang-Feng Zhou
The biodegradation of coal is an effective way of clean and efficient utilization of coal. However, how to improve the degradation rate has been the focus of attention. The effect of combined surfactants Tr-Rh (Tr-octyl phenoxy poly ethoxy, Rh-rhamnolipid) on the degradation of Baolixile coal by Bacillus sp. XK1 was studied. The degradation experiment showed that compared with non-surfactant, the degradation rate of Tr-Rh increased by 25.4 % and the degradation time was shortened by 2 days. The bacterial growth characteristics experiment found that in the presence of Tr-Rh, the highest OD 600 of Bacillus sp. XK1 reached 1.311 and the alkaline protease activity was 93.09 U/mL, indicating that Tr-Rh promoted the growth of Bacillus sp. XK1 , secreting more alkaline proteases, and increasing the contact with coal. The surface property experiments of coal showed that, the contact angle and adsorption rate of coal sample were 16.2 ° and 68.3 % in the presence of Tr-Rh respectively, which indicated that Tr-Rh improved the hydrophilicity and charge of coal surface, and increased the adsorption of bacteria on the coal surface. Furthermore, the degradation products were characterized by GC–MS and FTIR. Compared with non-surfactant, the chain alkanes of the liquid bioproducts increased from 48.22 % to 66.10 % in the presence of Tr-Rh. FITR analysis indicated that Bacillus sp. XK1 , in the presence of Tr-Rh, degraded certain aromatic rings and side-chain groups in coal samples. This study provides a new method and theoretical basis for the efficient biodegradation of coal by microorganisms to produce chain alkanes.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Guoqiang Zhao,Jing Zhou,Yanning Tian,Qifeng Chen,Dongmei Mao,Jianchun Zhu,Xing Huang
Fomesafen is a diphenyl ether herbicide used to control the growth of broadleaf weeds in bean fields. The persistence, phytotoxicity, and negative impact on crop rotation associated with this herbicide have led to an increasing concern about the buildup of fomesafen residues in agricultural soils . The exigent matter of treatment and remediation of soils contaminated with fomesafen has surfaced. Nevertheless, the degradation pathway of fomesafen in soil remains nebulous. In this study, Bacillus sp. Za was utilized to degrade fomesafen residues in black and yellow brown soils. Fomesafen's degradation rate by strain Za in black soil reached 74.4%, and in yellow brown soil was 69.2% within 30 days. Twelve intermediate metabolites of fomesafen were identified in different soils, with nine metabolites present in black soil and eight found in yellow brown soil. Subsequently, the degradation pathway of fomesafen within these two soils was inferred. The dynamic change process of soil bacterial community structure in the degradation of fomesafen by strain Za was analyzed. The results showed that strain Za potentially facilitate the restoration of bacterial community diversity and richness in soil samples treated with fomesafen, and there were significant differences in species composition at phylum and genus levels between these two soils. However, both soils shared a dominant phylum and genus, Actinobacteriota, Proteoobacteria, Firmicutes and Chloroflexi dominated in two soils, with a high relative abundance of Sphingomonas and Bacillus . Moreover, an intermediate metabolite acetaminophen degrading bacterium, designated as Pseudomonas sp. YXA-1, was isolated from yellow brown soil. When strain YXA-1 was employed in tandem with strain Za to remediate fomesafen contaminated soil, the degradation rate of fomesafen markedly increased. Overall, this study furnishes crucial insights into the degradation pathway of fomesafen in soil, and presents bacterial strain resources potentially beneficial for soil remediation in circumstances of fomesafen contamination.
研究领域:
环境科学
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微生物学
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土壤学
·
农学
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DOI
PubMed
高影响因子
BIOCATALYSIS AND BIOTRANSFORMATION
·
2019
·
IF 1.627
Lu Dong,Yongkai Xu,Yun Zhang,Aijun Sun,Yunfeng Hu
Chiral 1-phenylethanol and its ester derivative are important chiral chemicals in diverse industries and the preparation of those optically pure enantiomers is of great importance. One bacterium, Bacillus sp. DL-2, whose extracellular proteases could efficiently asymmetrically hydrolyse (±)-1-phenylethyl acetate, was isolated from the deep sea of the Western Pacific. After the immobilization of extracellular proteases and the optimization of enzymatic reactions, (R)-1-phenylethanol was prepared with the enantiomeric excess (e.e.) being 97% and the yield being 41%, respectively. The optimal resolution reaction condition for the preparation of (R)-1-phenylethanol using immobilized extracellular proteases was found to be 5-mM (±)-1-phenylethyl acetate, 360 mg/mL immobilized extracellular proteases, pH 6.5, and 20 °C for 2 h. (S)-1-phenylethyl acetate was generated through enzymatic kinetic resolution with the e.e. being as high as 99% and the yield being 71%, respectively. The optimal resolution reaction condition for the preparation of (S)-1-phenylethyl acetate was found to be 2.5-mM (±)-1-phenylethyl acetate, 440 mg/mL immobilized extracellular proteases, pH 7.5, and 35 °C for 10 h. Our report is the first report about the kinetic resolution of (±)-1-phenylethyl acetate using proteases and the enantio-preference of the proteases was found to be the same as those of most other esterases/lipases. Also notably, the optical purity of (S)-1-phenylethyl acetate generated through kinetic resolution using the proteases of Bacillus sp. DL-2 was the highest report so far. Proteases from deep-sea Bacillus sp. DL-2 are new contributions to the biocatalyst library for the preparation of valuable chiral alcohols and chiral esters through kinetic resolution.
研究领域:
生物催化
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生物化学
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微生物学
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手性化学
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高影响因子
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
·
2020
·Vol. 113
·期 10
·1467-1477
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IF 2.674
Yin Yi-Rui,Sang Peng,Yang Feng-Li,Li Tao,Yang Run-Feng,Liu Hong-Yan,Luo Zong-Long,Li Wen-Jun,Yang Li-Quan
A β-glucosidase gene ( bsbgl1a ) from Bacillus sp. CGMCC 1.16541 was expressed in Escherichia coli BL21 and subsequently characterized. The amino acid sequence shared 83.64% identity with β-glucosidase (WP_066390903.1) from Fictibacillus phosphorivorans . The recombinant β-glucosidase (BsBgl1A) had a molecular weight of 52.2 kDa and could hydrolyze cellobiose, cellotriose, cellotetrose, p-nitrophenyl-β- d -glucopyranoside ( p NPG), and p-nitrophenyl-β- d -xylopyranoside ( p NPX). Optimal activity for BsBgl1A was recorded at 45 °C with a pH between 5.6 and 7.6, and 100% of its activity was maintained after a 24 h incubation between pH 4 and 9. Kinetic characterization revealed an enzymatic turnover (Kcat) of 616 ± 2 s −1 (with cellobiose) and 3.5 ± 0.1 s −1 (with p-nitrophenyl-β- d -glucopyranoside). Interestingly, the recombinant enzyme showed cupric ion (Cu 2+ ), sodium dodecyl sulfate (SDS) and alcohol tolerance at 10 mM for Cu 2+ and 10% for both SDS and alcohol. Additionally, BsBgl1A had high tolerance for glucose (Ki = 2095 mM), which is an extremely desirable feature for industrial applications. Following the addition of BsBgl1A (0.05 mg/ml) to a commercial cellulase reaction system, glucose yields from sugarcane bagasse increased 100% after 1 day at 45 °C. This work identifies a Cu 2+ , SDS, alcohol, and glucose tolerant GH1 β-glucosidase with potential applications in the hydrolysis of cellulose for the bioenergy industry.
研究领域:
酶学
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微生物学
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生物技术
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生物化学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Zhenkun Deng,Bing Hu,Changliu He,Lihe Zhang,Xi Zhao,Xu Zhang
Understanding the biodegradation of benzotriazole compounds (BTs) is crucial for improving their removal from the environment. Experiments using activated sludge showed a 61.65 % removal rate for benzotriazole (BTA), primarily through biodegradation. Notably, microbial diversity changed significantly, especially within the class Bacilli , which increased from 0.17 % to 0.75 % over 21 days ( p < 0.05). A novel Bacillus strain, DH-12, was identified, capable of degrading over 85 % of BTA and 5-methylbenzotriazole (MBT) within 24 hours. During the degradation of three BTs, seven possible intermediates were detected. Strain DH-12 appears to use methylation and carboxylation pathways for transformation and shows aerobic dechlorination ability. Additionally, this strain helps reduce the toxicity of BTs. This research enhances our understanding of BTs biodegradation by both microbial communities and a specific bacterial strain, offering valuable insights for environmental biotransformation strategies.
研究领域:
环境科学
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微生物学
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DOI
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2024
·
IF 4.1
Bo Zhang,Lei Wang,Zhenjun Diwu,Maiqian Nie,Hongyun Nie
A strain of Bacillus licheniformis T5 was isolated from soil contaminated with crude oil due to its efficient degradation of polycyclic aromatic hydrocarbons (PAHs). When subjected to stress metabolism using phenanthrene as a carbon source, significant changes were observed in T5 cells. Infrared spectrum analysis revealed the presence of -C=C- and Ph-O-C (aromatic) groups on the bacterial surface, facilitating the adsorption of PAHs on the phospholipid layer and causing damage to the cell membrane. Scanning electron microscope (SEM) analysis showed the changes of cell morphology, including a large number of folds on the lower surface and the folding of cell membrane. Transmission electron microscope (TEM) observation showed that non-stressed bacteria with adequate nutritional conditions accumulated more lipids. However, the stress group contained more protein. It was found that stress metabolism led to the increase of protein content in T5 cells by 16.4% and the activity of oxidoreductase more than doubled. These physiological and biochemical changes enhance the ability of stressed bacteria to degrade PAHs efficiently, thereby reducing the degradation cycle. The findings offer valuable insights for the remediation of PAHs pollution.
研究领域:
微生物学
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环境科学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xiaoxian Cai,Ke Chen,Lixue Zheng,Huijun Guan,Tianlong Hao,Wenqing Zhou,Jingjing Yang,Lihua Qi,Chunfeng Guan
The widespread accumulation of organic pollutants in agricultural soils, such as dimethyl phthalate (DMP), poses serious risks to human health and ecological safety. The application of plant growth-promoting rhizobacteria (PGPR) to improve phytoremediation efficiency for organic pollutant-contaminated soils has gained increasing attention. However, synergistic PGPR-plant signaling and competitive root colonization of PGPR directly affect the remedial efficiency of the PGPR-plant system for soil organic pollutants. Acyl-homoserine lactones (AHLs), one of the quorum sensing (QS) signals, can enhance microbial cooperation and promote plant growth, thereby probably facilitating phytoremediation processes for pollutant degradation. In this study, the potential capacity of AHLs to assist PGPR in the phytoremediation of DMP-polluted soils was explored, with a focus on their effects on DMP removal, plant physiological functions, and rhizosphere microbial community structure. The results showed that AHLs (C 4 -HSL)-assisted PGPR ( Bacillus YT2) treatment significantly increased DMP removal to 92.60 % and improved plant growth. Compared to the individual AHLs or PGPR treatments, the AHLs-assisted PGPR more effectively enhanced plant photosynthesis, osmoregulation, and antioxidant capacity, reduced oxidative damage, and improved soil enzyme activity and nutrient availability. Additionally, the AHLs-assisted PGPR treatment modulated microbial community in the rhizosphere, increasing the richness of pivotal functional taxa such as Xanthomonadaceae and Acidobacteriota. AHLs might directly promote DMP degradation by regulating the QS system and enhancing plant-bacterial communication, while also indirectly supporting plant growth through stress tolerance enhancement. In conclusion, AHLs-mediated enhancement of PGPR functionality provides a promising approach for remediating DMP-contaminated soils and offers a theoretical basis for developing composite microbial formulations.
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高影响因子
MOLECULAR PLANT-MICROBE INTERACTIONS
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2024
·
IF 3.2
Yanning Tian,Fangya Zhong,Na Shang,Houyu Yu,Dongmei Mao,Xing Huang
Diphenyl ether herbicides are extensively utilized in agricultural systems, but their residues threaten the health of sensitive rotation crops. Functional microbial strains can degrade diphenyl ether herbicides in the rhizosphere of crops, facilitating the restoration of a healthy agricultural environment. However, the interplay between microorganisms and plants in diphenyl ether herbicides degradation remains unclear. Thus, the herbicide-degrading strain Bacillus sp. Za and the sensitive crop, maize, were employed to uncover the interaction mechanism. The degradation of diphenyl ether herbicides by strain Bacillus sp. Za was promoted by root exudates. The strain induced root exudates re-secretion in diphenyl ether herbicide-polluted maize. We further showed that root exudates enhanced the rhizosphere colonization and the biofilm biomass of strain Za, augmenting its capacity to degrade diphenyl ether herbicide. Root exudates regulated gene fliZ, pivotal in biofilm formation. Wild-type strain Za significantly reduced herbicide toxicity to maize compared to the ZaΔfliZ mutant. Moreover, root exudates promoted strain Za growth and chemotaxis, which was related to biofilm formation. This mutualistic relationship between the microorganisms and the plants demonstrates the significance of plant-microbe interactions in shaping diphenyl ether herbicide degradation in rhizosphere soils.
研究领域:
环境微生物学
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植物科学
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农业化学
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微生物生态学
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生物修复
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2018
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IF 2.829
Leila Abdoli,Chunhai Guo,Xiuyong Chen,Xiaoyan He,Hua Li
Formation of microbial biofilm on marine structures is crucial for subsequent attachment of macro fouling species and understanding the impact of fluid conditions on formation and growth of the biofilm is essential for preventing biofouling. Here we report the effect of shear stresses generated by laminar and weakly turbulent flow on the adhesion of typical marine bacterium Bacillus sp. biofilm on stainless steel . Numerical simulation was carried out to calculate the distribution of pressure and shear stress applied on adhered bacteria. The shear stress was proportional to both the distance from the center of rotating disk and rotation speed. The stress reached 18 Pa as the flow velocity was 2.4 m/s and the rotation speed was 600 rpm. Further adhesion testing revealed that development of the bacterial biofilm in early stage was unaffected by the shear stresses. However, shear stress regulated the adherence orientations of the bacteria on the steel surface. Turbulent flow obviously shaped development of the architecture of the biofilm, as revealed by CLSM and SEM characterization. Biofilm growth patterns were clearly observed under weakly turbulent flow, however, they were not seen under laminar flow . The microcolonies assumed elongated forms, possibly triggered by exerted pressure drag force. The results give insights into possibly controlling the formation of marine biofilm on marine infrastructures for desired antifouling performances.
研究领域:
微生物学
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海洋科学
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材料科学
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2015
·Vol. 178
·期 3
·558-575
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IF 1.735
Liang Jiayuan,Zhang Yun,Sun Aijun,Deng Dun,Hu Yunfeng
A novel microbial esterase BSE01281 identified from the Indian Ocean was cloned, expressed, and functionally characterized. Esterase BSE01281 could enanoselectively resolve (±)-1-phenylethanol and (±)-1-phenylethyl acetate through two types of enzymatic reactions. After the optimization of enzymatic reactions, BSE01281 could efficiently generate ( R )-1-phenylethyl acetate with high enantiomeric excess (>99 %) and high conversion (42 %) after 96 h trans-esterification reactions. Additionally, BSE01281 could also produce ( R )-1-phenylethanol ( e.e . > 99 %) and ( S )-1-phenylethyl acetate ( e.e . > 95 %) at a conversion of 49 % through direct hydrolysis of inexpensive racemic 1-phenylethyl acetate for 8 h. Optically pure ( R )-1-phenylethanol generated from direct enzymatic hydrolysis of racemic 1-phenylethyl acetate by BSE01281 is not easily prepared by dehydrogenases, which generally follow the “Prelog’s rule” and give ( S )-1-phenylethanol instead.
研究领域:
生物化学
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酶工程
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合成生物学
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DOI
PubMed
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 2
·126
·
IF 4.073
Chunrui Ma,Xiao Li,Kun Yang,Shangyong Li
Chitooligosaccharide (COS) has been recognized to exhibit efficient anti-oxidant activity. Enzymatic hydrolysis using chitosanases can retain all the amino and hydroxyl groups of chitosan, which are necessary for its activity. In this study, a new chitosanase encoding gene,csnQ, was cloned from the marineBacillussp. Q1098 and expressed inEscherichia coli. The recombinant chitosanase, CsnQ, showed maximal activity at pH 5.31 and 60 °C. Determination of CsnQ pH-stability showed that CsnQ could retain more than 50% of its activity over a wide pH, from 3.60 to 9.80. CsnQ is an endo-type chitosanase, yielding chitodisaccharide as the main product. Additionally, in vitro and in vivo analyses indicated that chitodisaccharide possesses much more effective anti-oxidant activity than glucosamine and low molecular weight chitosan (LMW-CS) (~5 kDa). Notably, to our knowledge, this is the first evidence that chitodisaccharide is the minimal COS fragment required for free radical scavenging.Keywords:chitosanase;pH-stability;Bacillussp. Q1098;chitooligosaccharide;anti-oxidant activity
研究领域:
微生物学
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生物化学
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酶学
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药学
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产品关联
DOI
PubMed
高影响因子
BIOCATALYSIS AND BIOTRANSFORMATION
·
2021
·
IF 2.181
Lu Dong,Shujuan Qi,Jianwei Jia,Yun Zhang,Yunfeng Hu
Bacillus sp. DL-1 was isolated from the deep sea of the Western Pacific Ocean and behaved very good resistance to NaCl. The extracellular proteases of Bacillus sp. DL-1 were found to exhibit excellent enantioselectivity for the kinetic resolution of (±)-1-phenylethyl acetate. To improve the stability of the enzyme, the immobilized extracellular proteases were preparated by using 60 g kieselguhr and 1-L crude fermentation broth containing extracellular proteases of Bacillus sp. DL-1, shaking at 25 °C, 200 r/min for 10 h. Every gram of kieselguhr adsorbed 25.7 mg extracellular protease and the enzymatic activity recovery was 79.86%. The immobilized proteases preserved about 35.8% of its activity after 6 repeated uses, which were also used as biocatalyst to asymmetrically hydrolyse (±)-1-phenylethyl acetate for the preparation of (R)-1-phenylethanol and (S)-1-phenylethyl acetate with high optical purities. The effects of pH, temperature, enzyme concentration, substrate concentration, reaction time and additives (metal ions/surfactants) on the resolution were investigated by single factor experiments. Under the optimal reaction conditions (10 mM (±)-1-phenylethyl acetate, 40 mg/mL immobilized extracellular proteases, pH 7.5 (Tris-HCl), 5% (v/v) methanol and 45 °C for 2 h), (R)-1-phenylethanol was generated with the e.e.p being > 97%, and the yield being 53%, respectively. Analogously, under the optimal reaction conditions (10-mM (±)-1-phenylethyl acetate, 360 mg/mL immobilized extracellular proteases, pH 6.0 (PB), 5% (v/v) DMSO and 35 °C for 1.5 h), (S)-1-phenylethyl acetate was generated with the e.e.s being over 99% and the yield being 79%, respectively. Compared with the extracellular proteases from Bacillus sp. DL-2, the immobilized extracellular proteases from Bacillus sp. DL-1 exhibited higher hydrolytic activity and could asymmetrically hydrolyse (±)-1-phenylethyl acetate by using higher substrate concentrations, shorter reaction times to obtain higher yields. Notably, the extracellular proteases of Bacillus sp. DL-1 were demonstrated to behave the same enantio-preference as those of most other reported esterases/lipases. Proteases from deep-sea Bacillus sp. DL-1 are promising biocatalysts for the synthesis of valuable chiral chemicals.
研究领域:
生物催化
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酶学
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微生物学
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有机化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）