POLYMER
·
2023
·
IF 4.6
Yinjie Qian,Junjie Zhang,Yanxue Yu,Qiushi Jiang,Bin Yan,Xinlong Song,Xiaobin Yu,Zhiqiang Cheng
Methyl eugenol (ME) is a highly potent sexual attractant for Bactrocera dorsalis , which plays a crucial role in agricultural pest management. In this study, a composite fiber membrane was prepared by polystyrene (PS), polyethylene glycol diacrylate (PEGDA), and ME through electrostatic spinning respectively. The effective release period of ME was prolonged by triggering PEGDA with UV light. The mass ratio of PS to PEGDA affects the morphology of the fibers as well as the loading capacity (LC) and encapsulation efficiency (EE) for ME. Under optimal test conditions, the diameter of the fibers was 1.70 μm, and the ME loading capacity and encapsulation efficiency were (7.80 ± 0.07%) and (85.84 ± 7.94%). The combination of PS and PEGDA was confirmed by FTIR analysis, while its excellent thermal stability was verified through TGA testing. In the PS/PEGDA-ME-UV membrane, the cumulative release rate of ME reached 81.97% by week 8, which conformed to a first-order model. In the trapping experiments, a 53% rate of Bactrocera dorsalis capture was maintained even by week 8, demonstrating its potential for application in agricultural biocontrol.
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Life-Basel
·
2024
·Vol. 14
·期 6
·713
·
IF 3.2
Leyuan Liu,Lang Yang,Jinxi Yuan,Jie Zhang,Chenhao Liu,Hongxu Zhou,Wei Liu,Guirong Wang
The oriental fruit fly,Bactrocera dorsalis(Hendel), poses a significant threat to the global fruit industry, causing damage to diverse fruits like citrus, mango, and guava. Chemical pesticides have limited effectiveness, and pesticide residues and pesticide resistance are pressing issues. Therefore, it is essential to develop environmentally friendly pest control methods to address this problem. Behavior-modifying chemicals, including male attractants and intersex protein baits, play a critical role in the control ofB. dorsalis. The mature host fruit serves as both an oviposition site and food source under natural conditions, making it a potential attraction source for oriental fruit flies. Orange,Citrus sinensis, is a main host ofB. dorsalis, and commercial orange juice is a common attractant for the egg laying ofB. dorsalis. Although it can both attract and elicit oviposition behaviors inB. dorsalisadults, its active components are still unclear. This study utilized analytical chemistry, behavioral tests, and electrophysiology to identify the active components of commercial orange juice that attractB. dorsalis, with the aim of providing a reference for the development of behavior-modifying chemical-based techniques to controlB. dorsalis.Five compounds with a high abundance were identified via a GC-MS, including D-Limonene, butanoic acid ethyl ester, β-myrcene, linalool, and α-terpineol. Behavioral and electrophysiological experiments uncovered that D-Limonene was the active substance that was the main attractant in the mixture of these five substances, evoking a strong electrophysiological response in adultB. dorsalis. D-Limonene strongly attracts adultB. dorsalisonly when they are sexually mature, and the attraction is not rhythmic. Olfaction plays a leading role in the attraction of D-Limonene to adultB. dorsalis, andOrco−/−mediates the perception of D-Limonene byB. dorsalis. Overall, D-Limonene is one of the key attractant compounds forB. dorsalisin the volatile compounds of commercial orange juice, offering possible support for the development of behavior-modifying chemical-based technology to controlB. dorsalisin the future.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Mobarak Syed Husne,Basit Abdul,Wu Lei,Khurshid Aroosa,Gui Shun-Hua,Hu Chao-Xing,Liu Tong-Xian
Bactrocera dorsalis is a major pest causing economic losses in fruit and vegetable production. Current control methods, such as synthetic pesticides and male annihilation techniques, have drawbacks, including health risks, resistance development, and ineffectiveness against females. This study investigates the attractions and oviposition stimulant activities of five common volatiles—[isobutyl acetate (A), isoamyl acetate (B), isobutyl butyrate (C), isoamyl isovalerate (D), and isoamyl butyrate (E)]—from the most preferred hosts: mango, guava, orange, and banana. These volatiles were tested at three different concentrations, both individually and in complex blends. Choice tests were performed using a Y-tube olfactometer and Petri plate with agarose assays, and antennal responses were determined using electroantennogram (EAG) recordings. Among them, a blend comprising 1.25% isoamyl acerate (10.95 µg/µl), 1.25% isoamyl isovalerate (10.75 µg/µl), and 20% isoamyl butyrate (172.40 µg/µl) (B1D1E3) emerged as the most potent attractant for both sexes and oviposition stimulant for females. Moreover, combining B1D1E3 with mango, banana, or orange juice significantly enhanced egg-laying behavior. Electrophysiological recordings supported these findings, with B1D1E3 eliciting the strongest antennal responses. Results suggest B1D1E3 could be used to elicit strong oviposition responses in mass rearing of B. dorsalis for the Sterile Insect Technique.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Zhao Liu,Qian-Ping Xie,Huai-Wang Guo,Wei Xu,Jin-Jun Wang
Odorant-binding proteins (OBPs) are believed to play critical roles in host-seeking behavior. However, little attention was paid to its different functions in male and female. The antenna-specific OBP gene from Bactrocera dorsalis , BdorOBP13, was cloned and its expression profile was examined. The results showed that BdorOBP13 was exclusively expressed in male and female adults, which exhibited a high transcript level in antennae. After injection of BdorOBP13 dsRNA, its transcript level in males and females decreased significantly. Electrophysiological responses of RNAi-injected flies to, methyl eugenol (male attractant) and γ-octalactone (female attractant) decreased significantly. However, no significant changes in the electrophysiological response were observed in RNAi-injected flies to benzothiazole, (+),dipentene, and ethyl tiglate. The behavioral bioassay showed that males treated with RNAi significantly reduced their preference to methyl eugenol, while RNAi-injected females showed a significantly lower preference to γ-octalactone, suggesting that BdorOBP13 may have different functions between males and females: it may be involved in the detection of methyl eugenol in males but is involved in the detection of γ-octalactone in females. These findings improve our understanding of insect OBPs and their roles in insect chemosensation, which may provide us with new molecular targets in the management of B. dorsalis .
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ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
·
2018
·Vol. 98
·期 1
·e21452
·
IF 1.316
Jing Zhang,Deye Luo,Peng Wu,Haozhe Li,Hongyu Zhang,Weiwei Zheng
Odorant-binding proteins (OBPs) in insects are essential for mating and oviposition host selection. How these OBPs respond to different hosts at the mRNA level and their effects on behavior remain poorly characterized. The oriental fruit fly Bactrocera dorsalis is a highly invasive agricultural pest with an extremely broad host range and high fecundity. Based on our previously constructed B. dorsalis transcriptome, six OBP s that were differentially expressed during three different physiological adult stages were identified. A phylogenetic tree was constructed to illustrate the relationships of these six OBP s with OBP sequences from three other dipteran species ( Drosophila melanogaster , Anopheles gambiae , and Ceratitis capitata ). The spatiotemporal expression profiles of the six OBP s were analyzed using quantitative real-time PCR. Our results revealed that OBP19c, OBP44a, OBP99a , and OBP99d were abundantly expressed from the prepupa stage to the adult stage, and most of the OBP s were mainly expressed in the head, wings, and antennae. The expression levels of these OBP s were upregulated when female flies were exposed to their preferred hosts. Silencing OBP99a resulted fewer eggs being laid compared with the control group when the females were exposed to their preferred host, that is, banana, whereas more eggs were laid when a non-preferred host, that is, tomato, was used. Furthermore, silencing OBP99a led to sexually dimorphic mating behavior. dsOBP99a -injected males dramatically reduced courtship, whereas enhanced courtship was observed in the treated females. These data indicate that OBP s may participate in different biological processes of B. dorsalis . Our study will provide insight into the molecular mechanism of chemoreception and help develop ecologically friendly pest-control strategies.
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Insects
·
2022
·Vol. 13
·期 11
·1004
·
IF 3.139
Yinjun Fan,Changzhen Zhang,Yu Qin,Xinhui Yin,Xinyi Dong,Nicolas Desneux,Hongxu Zhou
Simple SummaryBactrocera dorsalisis a highly invasive polyphagous pest of fruit and vegetable, causing severe economic loss and trade restrictions by ovipositing inside hosts. Cryptic feeding habits of larval stages, strong flight ability of adults and prevalence of insecticide resistance reduce the efficiency of chemical control. Currently, the most cost-effective tool is olfaction-based trapping. Methyl eugenol (ME), a naturally occurring compound in some plants, alone or combination with insecticides has been widely used as a male attractant to monitor and controlB. dorsalispopulations for seven decades. Intense ME selection under laboratory conditions has resulted in the evolution of non- responsiveness inB. dorsalisand field management strategies based on ME has failed to eradicate recolonization ofB. dorsalisin some islands. However, the levels of ME responsiveness inB. dorsalisfield populations in China is unknown. In this study, we found that field populations had lower ME sensitivity compared to the susceptible strain. Furthermore, the results of olfactory organs, gene expressions and the bioassays showed that odorant binding protein (BdorOBP2, BdorOBP83b) and P450 may be involved in the lower sensitivity. The findings will guide the use of lures combined with insecticides and help to exploit molecular targets for the development of new attractants.AbstractBactrocera dorsalisis a notorious polyphagous pest in China, and its management strategies largely depend on methyl eugenol (ME), which has been widely used as an attractant to monitor and eradicateB. dorsalispopulations for seven decades. However, the non-responsiveness levels in fieldB. dorsalispopulations to ME is unknown. In this study, we monitored the response to ME in field populations from the four most heavily infested provinces in China, and the results showed that the populations had lower sensitivity to ME relative to GZS susceptible strain. The percent responsiveness of the lowest sensitivity population was 5.88-, 3.47-, and 1.47-fold lower relative to the susceptible strain at doses of 1, 10, and 100 µL of ME, respectively. Gene expression analysis and inhibitor assays further revealed that odorant binding protein (BdorOBP2, BdorOBP83b) and the P450 enzyme system may be associated with the lower response to ME. To our knowledge, this work is the first to report that the P450 enzyme system confers a lower responsiveness to lure insects. These findings provided valuable insights for exploiting ME non-responsiveness to protect sterile males from ME-based control strategies and the use of lures combined with insecticides.Keywords:methyl eugenol;non-responsiveness;odorant binding protein;P450;trapping
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Jinxin Yu,Jiayi He,Xin Gong,Jimin Xu,Yongqiang Bai,Yiqiang Tian,Xilian Yang,Xinnian Zeng,Jiali Liu
Associative learning is central to studies of cognitive behavior in invertebrates, including insects. It involves acquiring new values from rewards and punishments by continuously updating sensory cues. Different biogenic amines guide the processes of reward and punishment. The role of dopamine (DA) signaling in associative learning has long been a focal point in the study of insect cognition. Previous studies across multiple insect species have established a general framework in which DA is primarily associated with aversive learning and punishment processing. However, emerging evidence suggests that DA has also been implicated in appetitive memory in certain species, indicating potential variability in the neuromodulatory mechanisms underlying associative learning. Here, we conducted behavioral assays, pharmacological interventions, and molecular manipulations to investigate the role of DA signaling in both aversive and appetitive classical conditioning in Bactrocera dorsalis , an economically important agricultural pest with well-documented learning and memory capabilities. We found that pharmacological blockade of DA signaling impaired aversive but not appetitive learning and memory, consistent with its conserved role in punishment processing. RNAi knockdown of DA receptors impaired both aversive learning and memory, reinforcing the pharmacological findings. Surprisingly, BdDOP2 knockdown impaired appetitive memory, uncovering a unique role for DA receptor subtype in appetitive memory. These findings provide new insights into the conserved and divergent functions of DA in insect cognition. Our results highlight B. dorsalis as a promising model for studying the neural mechanisms underlying associative learning, with potential applications in developing targeted pest control strategies by disrupting specific aminergic pathways involved in olfactory-based decision-making.
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Journal of Integrative Agriculture
·
2023
·
IF 4.8
Jin-xin YU,Yan-min HUI,Jia-yi HE,Ying-hao YU,Zheng-bing WANG,Si-quan LING,Wei WANG,Xin-nian ZENG,Jia-li LIU
The cAMP-dependent protein kinase A (PKA) signaling pathway has long been considered critical for long-term memory (LTM) formation. Previous studies have mostly focused on the role of PKA signaling in LTM induction by multiple spaced conditioning with less attention to LTM induction by a single conditioning. Here, we conducted behavioral-pharmacology, enzyme immunoassay and RNA interference experiments to study the role of the PKA signaling pathway in LTM formation in the agricultural pest Bactrocera dorsalis , which has a strong memory capacity allowing it to form a two-day memory even from a single conditioning trial. We found that either blocking or activating PKA prior to conditioning pretreatment affected multiple spaced LTM, and conversely, they did not affect LTM formed by single conditioning. This was further confirmed by enzyme-linked immunosorbent assay (ELISA) and silencing of the protein kinase regulatory subunit 2 and catalytic subunit 1. Taken together, these results suggest that activating PKA during memory acquisition helps to induce the LTM formed by multiple spaced conditioning but not by a single conditioning. Our findings challenge the conserved role of PKA signaling in LTM, which provides a basis for the greater diversity of molecular mechanisms underlying LTM formation across species, as well as possible functional and evolutionary implications.
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Journal of Integrative Agriculture
·
2024
·
IF 4.6
Jinxin Yu,Jiayi He,Xuefeng Zhang,Chuxiao Lin,Shiyan Liu,Xin Gong,Xinnian Zeng,Jiali Liu
To ensure the reliability of learned information, most insects require multiple intervals of experience before storing the information as Long-term memory (LTM), and this requirement has been validated in insects from the behavioral to the molecular level. Recent studies have shown that some insects can form LTM after a single experience, although the mechanisms underlying one-trial LTM formation are not well understood. Therefore, understanding the mechanisms underlying rapid learning and subsequent preference formation in insects is crucial. Here we show that the agricultural pest Bactrocera dorsalis can rapidly form LTM, which is dependent on protein synthesis, and that the formation of LTM requires high energy support at the cost of reduced survival. Furthermore, based on a liquid chromatography-mass spectrometry (LC-MS) metabolomics approach, we found that LTM-related processes are sequentially coupled to two processes for energy generation, the TCA cycle and oxidative phosphorylation. This was further confirmed by blocking these energy generation processes. Our results provide a theoretical basis for the development of behavioral modulators in oriental fruit flies that target energy generation intermediate metabolites, as well as a new perspective on the rapid formation of LTM in insects.
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PEST MANAGEMENT SCIENCE
·
2022
·Vol. 79
·期 2
·666-677
·
IF 4.462
Meng-Ling Chen,Shu-Xia Zhang,Peng-Yu Guo,Qing-Shi Qin,Li-Wei Meng,Guo-Rui Yuan,Jin-Jun Wang
BACKGROUND The oriental fruit fly, Bactrocera dorsalis (Hendel) is a worldwide pest damaging a wide range of hosts. Due to the long-term indiscriminate use of insecticides, B. dorsalis has developed serious resistance to several insecticides. UDP-glycosyltransferases (UGTs) are secondary metabolic enzymes involved in biotransformation and play an important role in the metabolism of plant secondary metabolites and synthetic insecticides in insects. Thus, we suspect that UGTs in B. dorsalis play an important role in insecticide tolerance. RESULTS In this study, 31 UGT genes were identified in the genome of B. dorsalis , belonging to 13 subfamilies. Real-time quantitative polymerase chain reaction (RT-qPCR) results revealed that 12 UGT genes were highly expressed in the antennae, midgut, Malpighian tubule and fat body. The mRNA expressions of 17 UGT genes were up-regulated upon exposure to λ-cyhalothrin, imidacloprid, abamectin and chlorpyrifos. Knockdown of the selected five UGT genes ( BdUGT301D2 , BdUGT35F2 , BdUGT36K2 , BdUGT49D2 , BdUGT50B5 ) by RNA interference increased the mortality of B. dorsalis from 9.29% to 27.22% upon exposure to four insecticides. CONCLUSION The abundance of UGTs in B. dorsalis is similar to other insect species, and 12 out of 31 UGTs were specifically expressed in metabolic tissues, suggesting a key role in detoxification. Down-regulation of five selected UGT genes increased the susceptibility of B. dorsalis to various insecticides, indicating that UGTs may play an important role in tolerance of B. dorsalis to multiple insecticides. © 2022 Society of Chemical Industry.
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Scientific Reports
·
2020
·Vol. 10
·期 1
·1-11
·
IF 3.998
Zhou You,Qin De Qiang,Zhang Pei Wen,Chen Xiao Tian,Liu Ben Ju,Cheng Dong Mei,Zhang Zhi Xiang
Azadirachtin exhibits excellent bioactivities against several hundred arthropods. However, current knowlege of its biochemical effect on B . dorsalis larvae is not deep enough. In this study, integrated LC-MS and GC-MS-based untargeted metabolomics were used to analyze the changes of endogenous metabolites and the biochemical effects of azadirachtin on B . dorsalis larvae. Azadirachtin has excellent bioactivities against B . dorsalis larvae in this study, leading to a longer developmental duration, lower survival rate, and low pupa weight. The effect of azadirachtin was investigated on a total of 22 and 13 differentially abundant metabolites in the LC–MS and GC–MS-based metabolomics results, are selected respectively. Pathway analysis indicated that 14 differentially enriched metabolic pathways, including seven influential pathways, are worthy of attention. Further integrated key metabolic pathway analysis showed that histidine metabolism, d -glutamine and d -glutamate metabolism, biotin metabolism, ascorbate and aldarate metabolism, pentose and glucuronate interconversions, and alanine, aspartate and glutamate metabolism in B . dorsalis larvae are significantly relevant pathways affected by azadirachtin. Although extrapolating the bioactivity results in this study to the practical project of B . dorsalis pest management in the field has limitations, it was found that azadirachtin has a significant effect on the primary metabolism of B . dorsalis larvae.
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PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Wei Zhao,Di Zou,ZhengYang Wang,GuiQiang Wang,HongBo Jiang,Guy Smagghe,JinJun Wang,DanDan Wei
BACKGROUND Bactrocera dorsalis (Diptera: Tephritidae) is a highly adaptable invasive pest that causes significant economic losses. Long-term chemical control strategies have led to high levels of insecticide resistance and severe environmental risks. C-type lectins (CTLs), key pattern recognition receptors in insect innate immunity, are named for their calcium-dependent carbohydrate recognition domains (CRDs) and they play a critical role in detecting and binding to foreign pathogens. This study aims to identify B. dorsalis CTLs (BdCTLs) and investigate their roles in pathogen defense. RESULTS A total of 42 BdCTL genes were identified. Based on the number of CRDs, 36 genes were classified into the S subfamily, while the remaining six were assigned to the X subfamily. Spatio-temporal expression analysis by qPCR revealed that BdCTL genes were expressed throughout all developmental stages with a higher expression observed in the midgut, Malpighian tubules, and fat body. The expression levels of BdCTL-S4 , BdCTL-S12 , and BdCTL-S31 were significantly upregulated under the infection of the entomopathogenic fungus Beauveria bassiana . Notably, BdCTL-S12 also exhibited upregulated expression patterns in response to four different pathogens bacteria with sustained expression over time. Using RNA interference (RNAi), we successfully knocked down BdCTL-S12 expression, achieving a silencing efficiency of 68%. Survival assays demonstrated a 10–30% reduction in the survival rate of B. dorsalis after pathogen infection following dsRNA injection compared to the control group. Further in vitro experiments confirmed that BdCTL-S12 can agglutinate and bind to pathogens in a calcium-dependent manner. This binding was competitively inhibited by polysaccharides, underscoring a key functional mechanism of BdCTL-S12 in pathogen recognition. CONCLUSION In summary, we identified 42 BdCTLs and demonstrated that BdCTL-S12 is critical for pathogen recognition and immune defense in B. dorsalis . These results advance our understanding of the molecular mechanisms underlying B. dorsalis immunity. Specifically, elucidating the role of CTLs may facilitate the development of microbial-based biological control strategies (e.g., entomopathogenic fungi or bacteria) against this invasive pest. Additionally, RNAi-mediated knockdown of CTL-encoding genes could weaken the immune system of Bactrocera flies, increasing their susceptibility to microbial infections and thereby enhancing the effectiveness of biological control. © 2025 Society of Chemical Industry.
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