APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 11
·5274-5291
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IF 3.094
Lei Jiping,Tang Kexin,Zhang Ting,Li Yan,Gao Zhen,Jia Honghua
2-O-α-D-Glucosyl glycerol (2-αGG) can be used as a multipurpose anti-aging, cell-stimulating, and skin moisturizing agent in the cosmetic industry. Sucrose phosphorylase (SPase) has been widely used in the production of 2-αGG. In this paper, the gene encoding sucrose phosphorylase from Bifidobacterium longum (BlSP) was inserted into pRSF-Duet-1 to construct the recombinant plasmid pRSF-BlSP and was functionally expressed in E. coli BL21(DE3) to be used as a biocatalyst for the synthesis of 2-αGG firstly. The mutations of BlSP were carried out based on alanine scanning, and a positive mutant G293A with a 50% increase in activity for 2-αGG production was identified. Mutant G293A has less K m and bigger k cat / K m towards glycerol than the parental BlSP. Subsequently, the production of 177.6 g/L 2-αGG was attained from 1 M sucrose and 1.2 M glycerol catalyzed by 17 mg/mL G293A mutant. This study indicated that BlSP has good potential in the production of 2-αGG.
研究领域:
生物技术
·
酶学
·
药学
·
化妆品
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Yiming Ren,Liang Li
Probiotics have numerous biological functions and clinical studies have reported that consuming probiotic foods could promote hair growth and hair follicle health. Bifidobacterium longum BB536 was selected to investigate the possibility of treating hair loss with Cell-Free Supernatant (CFS) of B. longum BB536 and the alleviation mechanism of CFS on dihydrotestosterone (DHT)-damaged human dermal papilla cells (HDPCs). The results showed that HDPCs treated with 10% CFS of B. longum BB536 for 24 h could significantly promote cell proliferation, repair cell damage, accelerate the process of cell cycle and increase the secretion of VEGF. The CFS of B. longum BB536 reduced the apoptosis rate of DHT-damaged HDPCs. In addition, it could reduce the level of pro-inflammatory factors and improve the alkaline phosphatase (ALP) activity. Metabolites of B. longum BB536 may increase the protein expression of Wnt10b, β-catenin and LEF-1 by activating Wnt/β-catenin signaling pathway and inhibit the expression of DHT-induced negative feedback factors DKK1 and GSK-3β, which alleviates the damage of DHT on HDPCs, some protein expression levels such as LEF-1 are close to minoxidil (common drugs for hair loss). So, metabolites of B. longum BB536 can be used as new treatment for hair loss due to its safety. This study provides theoretical basis for probiotics to prevent androgenic alopecia.
研究领域:
微生物学
·
细胞生物学
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药理学
·
皮肤病学
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产品关联
DOI
高影响因子
Food & Function
·
2019
·Vol. 10
·期 8
·4975-4984
·
IF 3.241
Hui Zhu,Yan Zhou,Yan Qi,Rui Ji,Junliang Zhang,Zhixiang Qian,Chunzhen Wu,Jun Tan,Lei Shao,Daijie Chen
The aim of this study was to investigate the characteristics of a novel selenium-enriched Bifidobacterium longum DD98 (Se-B. longum DD98) supplement food and its repairing effects on the intestinal ecology of mammals. We assessed the growth, Se accumulation, and Se biotransformation of B. longum DD98 and its effects on antibiotic-induced intestinal dysbacteriosis in mice. The viable bacterial count at the end of fermentation was not significantly affected by the presence of Se. Bifidobacterium longum DD98 took up inorganic Se from the medium and biotransformed it into Se-containing proteins and selenoamino acids. The dominant Se species was selenomethionine (SeMet), which comprised 87% of the total Se in Se-B. longum DD98. Furthermore, Se-B. longum DD98 showed better regulation of the disrupted intestinal microbiota back to normal levels and repaired damaged colon tissues compared to the natural recovery and B. longum DD98 treatments. These findings suggest that B. longum DD98 efficiently biotransformed inorganic Se into more bioactive organic Se forms and may have therapeutic potential for the restoration of antibiotic-induced intestinal dysbacteriosis.
研究领域:
微生物学
·
营养学
·
医学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Dan Li,Yue Su,Yuhang Meng,Cuicui Duan,Fumin Ma,Byung-Hoo Lee,Xiaolei Li
Mutants E395D and S321D showed 1.6× and 1.3× higher catalytic efficiency than BlGBE. • S321D/E395D-modified pea starch had further reduced retrogradation and digestibility. • E395D/S321D increased DP 2 and 3 short branches, reduced R₁₀₄₇/₁₀₂₂ and crystallinity. • S321D-modified cake had further improved slowly digestible starch and reduced hardness. • S321D/E395D had enhanced substrate binding and stabilized transition-state by H-bonds. The 1,4-α-glucan branching enzyme found in Bifidobacterium longum can modify the physicochemical properties of starch by forming denser and shorter branches. To enhance its activity and modification function with regard to pea starch, ten mutants were engineered using site-directed mutagenesis based on multiple sequence alignment, molecular docking, and dynamic simulation. The mutants E395D, S321D and M509L exhibited specific activities 1.32-, 1.23- and 0.81-fold higher than the wild type (WT). The catalytic efficiencies of S321D and E395D were 18.36 and 14.00 mg −1 ·min −1 ·mL, respectively, which were significantly higher than the WT's efficiency of 11.19 mg −1 ·min −1 ·mL. Docking analysis revealed that the mutations reorganised the hydrogen-bonding and electrostatic microenvironment at the active site, producing mutation-specific effects on substrate binding and transition-state stabilisation. These locally altered polar and nucleophilic properties ultimately dictated the catalytic efficiency of each mutant. Compared with WT, E395D and S321D increased DP 2 and 3 short branches, reduced FT-IR R₁₀₄₇/₁₀₂₂ and XRD crystallinity at 200–800 U/g starch, which expanded the amorphous region of starch. After seven days' storage at 4 °C, the retrogradation enthalpy of S321D-modified starch was 0.63 J/g lower than that of WT-modified starch of 0.71 J/g. Glucose release from S321D-modified starch was significantly lower than from WT-modified starch at the level of small intestinal α-glucosidase. The slowly digestible fraction of S321D-modified starch was 38.51%, significantly higher than the 29.19% observed in WT-modified starch. Fresh and stored pea cake modified by S321D had significantly lower hardness and higher slowly digestible properties than the WT-modified equivalent. Download: Download high-res image (208KB) Download: Download full-size image
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 30
·23495-23508
·
IF 6.7
Huang Zheng,Zhang Qing,Zhao Jianxin,Stanton Catherine,Ross R. Paul,Chen Wei,Yang Bo
Atopic dermatitis (AD) is a prevalent
early childhood inflammatory
skin disease frequently linked to gut dysbiosis. While Bifidobacterium longum subsp. infantis is a pioneer infant gut colonizer driving immune maturation, its
role in AD prevention remains unclear. Here, we investigated early
life intervention with B. longum subsp. infantis CCFM1269 using a 2,4-dinitrofluorobenzene
(DNFB)-induced murine AD model. Oral administration of CCFM1269 significantly
ameliorated AD symptoms, reducing skin/ear thickness, dermatitis scores,
and serum IgE. Mechanistically, CCFM1269 modulated the systemic Th1/Th2
balance by downregulating TSLP, IL-4, and IL-13, while upregulating
IFN-γ. Notably, CCFM1269 produced indole-3-lactic acid (ILA).
Via the gut–skin axis, ILA activated the aryl hydrocarbon receptor
(AHR) and its target CYP1A1 in skin tissues, strengthening the epidermal
barrier. Additionally, AD mitigation was associated with gut microbiota
reshaping. Ultimately, CCFM1269 alleviates AD primarily through the
ILA- AHR signaling pathway, highlighting its potential as a functional
food ingredient for early life AD prevention.
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Hongliang Zeng,Yixin Zheng,Yan Lin,Cancan Huang,Shan Lin,Baodong Zheng,Yi Zhang
The aim of this study was to investigate the proliferation effect of fractionated lotus seed resistant starch (LRS3-20%, LRS3-30%) and the structural changes following fermentation. Fractionated lotus seed resistant starch, especially LRS3-20%, promoted the proliferation of these probiotics, and Bifidobacterium longum produced large quantities of acetic acid, while Lactobacillus delbrueckii subsp. bulgaricus produced lactic acid. Additionally, the probiotics with lotus seed resistant starch (LRS3) and LRS3-20% survived more successfully in simulated gastrointestinal solutions compared to glucose, LRS3-30% and high amylose maize starch, but LRS3 and LRS3-20% differed in their abilities to enhance the tolerance to acidic buffer, bile acid and simulated gastric juice. Furthermore, after fermentation, fractionated lotus seed resistant starch displayed a loose, rough surface. The crystalline and double helical structure increased as the amount of amorphous and sub-crystalline structure reduced following fermentation. Interestingly, structural changes were correlated with the original structures and utilisation by the probiotics.
研究领域:
食品科学
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微生物学
·
营养学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）