FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Ruixing Zhang,Wenjing Yang,Qiming Pan,Qi Zeng,Chengtai Yan,Xue Bai,Yao Liu,Lugang Zhang,Baohua Li
The aim of this study was to investigate the impact of long-term exposure to blue light-emitting diodes (LEDs) on the accumulation of indolic glucosinolates and carotenoids, as well as the plant growth and antioxidant activities in both orange and common Chinese cabbage ( Brassica rapa L. ssp. pekinensis ). Blue light treatment also induced higher ferric-reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl by 20.66 % and 30.82 % and antioxidant enzyme activities catalase, peroxidase, superoxide dismutase, and the accumulation of non-enzymatic antioxidant substances (total phenols and total flavonoids) in the orange Chinese cabbage. Furthermore, long-term exposure to blue light had negative effects on the net photosynthetic rate and chlorophyll fluorescence levels. Meanwhile, blue light promoted accumulation of Indol-3-ylmethyl glucosinolate (I3M), β-carotene, lutein and zeaxanthin due to the high expression of regulatory and biosynthetic genes of the above metabolic pathways. In particular, lycopene and β-carotene content in orange Chinese cabbage increased by 60.14 % and 65.33 % compared to the ones in common line. The accumulation of carotenoid and increasing antioxidant levels in the orange cabbage line was influenced by long-term blue light irradiation, leading to better tolerance to low temperature and drought stresses. The up-regulation of transcription factors such as BrHY5-2 , BrPIF4 and BrMYB12 may also contribute to the increased tolerance in orange Chinese cabbage to extreme environmental stresses. The BrHY5-2 gene could activate carotenoid biosynthetic genes and induce the accumulation of carotenoids. These findings suggested that long-term blue light irradiation could be a promising technique for increasing the nutrition value and enhancing tolerance to low temperature and drought stresses in Chinese cabbage.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Miao Cui,Yun Xie,Jianqiao Jiang,Qirui Yu,Yiwen Chen,Yongkang Li,Yue Hu,Weidong Cheng,Jianming Niu,Feifei Gao
To investigate the effect of different processing methods on the quality of turnips, the alteration in physicochemical parameters, phenols, VOCs, and non-volatile metabolites were determined in turnips after steaming, boiling, and microwave heating. The results showed that steaming and microwaving improved the hypolipidemic, hypoglycaemic, and antioxidant activities of turnips and retained more active substances. Processing significantly affected amino acids and phenols, and the proportion of umami amino acids and coumaric acid increased. Sulfur-containing volatile compounds were significantly reduced and flavor improved. Microwaving increased the isothiocyanate content and retained the unique flavor of turnips. Untargeted metabolomics showed a significant increase in amino acids, organic acids, and nucleosides after processing, with the maximum difference in boiled samples. This study provides a theoretical foundation for the processing of turnips.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Cong Men,Zhenwen Xie,Kaihe Li,Xin Xing,Zifu Li,Jiane Zuo
To protect agro-systems and food security, study on the effect of microplastics and heavy metals on edible plants is of great significance. Existing studies mostly used virgin microplastics to evaluate their effects on plants, effects of naturally aged microplastics and their combined effects with heavy metals are rarely explored. In this study, single and combined effect of polyethylene microplastics (PE, both virgin and naturally aged) and cadmium (Cd) on pakchoi under seedling and mature stages were analyzed from perspectives of growth inhibition, oxidative damage, nutrition content and soil enzyme activities. Results showed that inhibiting effects of naturally aged PE (PEa) on the growth of pakchoi were stronger than virgin PE (PEv), whereas co-contamination of PEa and Cd was less toxic than that of PEv and Cd. The co-contamination of PE and Cd could inhibit pakchoi dry biomass by over 85 %. Both single and combined contamination of PE and Cd promoted soil fluorescein diacetate hydrolase (FDA) activities, which were 1.11 to 2.04 times of that in control group. Soluble sugar contents under co-contamination of PEa and Cd were 14 % to 22 % higher than those in control group. PEa and PEv showed different effects on oxidative damage of pakchoi. Compared with PEv, catalase (CAT) activities were more sensitive with PEa, whereas PEa had lower effect on superoxide dismutase (SOD) activities. The response of pakchoi to PE and Cd changed with growth stage. Chlorophyll contents in pakchoi under seedling stage were generally higher than those under mature stage. For Cd contaminated soils, PE benefited pakchoi growth under seedling stage, i.e. antagonistic effect between Cd and PE but hindered their growth under mature stage, i.e. synergistic effect. The results unraveled here emphasized PE, especially PEa, could trigger negative effects on agro-systems, whereas PE could be beneficial for heavy metal contaminated agro-systems under specific situations.
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PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 19
·3288
·
IF 5.1
Minmin Tang,Yongbiao Ni,Tianli Zang,Wei Gao,Jinzhu Song,Jie Zou,Danke Xu
An improved QuEChERS method based on magnetic nanomaterials was established, and coupled with ultra-high-performance liquid chromatography and a quadrupole electrostatic field orbitrap mass spectrometer, enabling non-targeted screening and quantitative analysis of commonly used pesticide and veterinary drug residues inBrassica rapa chinensis. The combination of magnetic PSA and magnetic multi-walled carbon nanotubes (MWCNTs) was utilized for purification of 121 organic compounds spanning a wide range of polarities. Spiking experiments showed that the developed non-target screening workflow exhibited convincing identification results, with over 89.3% of chemical substances being screened out even at spiking levels as low as 2 μg/kg. The linear ranges of these target substances were between 1 and 200 μg/kg, with determination coefficients all exceeding 0.99. The limits of quantification were between 2 μg/kg and 10 μg/kg. The average recovery rates were in the range of 61.7% to 121.6%. Nineteen compounds were confirmed (meeting all the restriction conditions) and quantified fromBrassica rapa chinensiscollected from vegetable-planting bases in Jiangsu Province. The results indicated that the magnetic bead-based pretreatment strategy can be utilized for the simultaneous screening and quantitative analysis of pesticide and veterinary drug residues inBrassica rapa chinensis.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wei Yi,Jiameng Shi,Wangting Zhou,Jinghong Wei,Yi Sun,Xiaoxiong Zeng,Wei Wang
In this study, the physicochemical properties, in vitro fermentation, and interaction with Bacteroides ovatus of a purified fraction of polysaccharides from the root of Brassica rapa (BRP2–2) were investigated. It was demonstrated with an in vitro anaerobic fermentation model that BRP2–2 significantly increased ( p < 0.05) the relative abundance of Bacteroides spp. After 24 h incubation of BRP2–2 with Bacteroides ovatus alone, 10.24 ± 0.69, 8.76 ± 0.48 and 3.37 ± 0.26 mM of acetate, propionate and isovalerate were produced, respectively. Moreover, transcriptome analysis of B. ovatus showed that 143 genes were up-regulated by BRP2–2, including five discrete polysaccharide utilization loci and two carbohydrate-active enzyme clusters. Based on the annotation of carbohydrate enzyme function, we speculate that BRP2–2 is a pectic polysaccharide containing homogalacturonan, rhamnogalacturonan I and rhamnogalacturonan II domains. These results suggested that BRP2–2 was degraded by B. ovatus , which produced metabolites with beneficial effects on host health.
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高影响因子
Biochemistry and Biophysics Reports
·
2026
·
IF 3.3
Wenyuan Wan,Yuntao Zhang,Xiaotong Yang,Jinyao Li,Jun Lu,Yu Zhao
This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H 2 O 2 -induced oxidative injury in PC-12 cells. Brassica rapa L. , a cruciferous plant rich in bioactive flavonoids, exhibits potent antioxidant and neuroprotective properties against oxidative stress, a key pathological driver in neurodegenerative diseases. This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H 2 O 2 -induced oxidative injury in PC-12 cells, employing a multi-omics approach integrating LC-MS/MS metabolite profiling, molecular docking, and transcriptomic validation. BREE demonstrated robust radical-scavenging activity. Molecular docking identified direct interactions among flavonoids within BREE, suggesting a role in redox modulation. Subsequent experiments confirmed the activation of the PI3K/Akt, Keap1-Nrf2, and cell cycle pathways. Functionally, BREE significantly restored cell viability to 162.3% ± 6.9% of that in the control group. These findings validate BREE's dual action in scavenging ROS and reprogramming redox signaling networks, positioning it as a candidate for combating oxidative stress-associated neurodegeneration and as a potential functional food for age-related cognitive decline.
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高影响因子
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