Systems Microbiology and Biomanufacturing
·
2025
·
IF 2.9
Shi Yu,Li Huifan,Lu Yuqi,Chen Ming,Yang Hua,Fu Dongmei
A simple and robust reversed-phase high performance liquid chromatography procedure was developed with only an aqueous H 3 PO 4 solution (pH 2.1) as the mobile phase for the simultaneous determination of nine organic acids (oxalic, formic, malic, lactic, acetic, citric, succinic, fumaric, and gallic acids) in beer based on a polar-copolymerized 3-chloropropyl C18 column. The proposed method was validated according to linearity, limits of detection, accuracy, precision, repeatability, and reproducibility. Quantifications of the nine organic acids in 12 commercial Chinese beer samples from five different brands were conducted only after degassing and filtration of the samples. In all beer samples, the most abundant organic acid was succinic acid; its concentration was in the range of 907.99–3557.22 mg/L. This was followed by malic acid (170.39–936.78 mg/L). Principal component analysis was used to compare the different brand of beers according to organic acid concentration, and results may provide a reference for the evaluation of beer quality and taste.
研究领域:
分析化学
·
食品科学
·
色谱技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 17
·4897-4907
·
IF 4.478
Yang Zhenglun,Chen Yihui,Jia Jianggang,Hou Chunyan,Xuan Rongrong,Wang Tingting
In the current study, the C18-modified halloysite was fabricated via silylation reaction and subsequently used as sorbent in matrix solid-phase dispersion (MSPD) for the extraction of bisphenol A and diethylstilbestrol from human placenta, followed by high-performance liquid chromatography-tandem mass spectrometry analysis. The as-prepared sorbent was characterized by scanning electron microscopy, energy-dispersive spectrometry, Fourier transform infrared spectroscopy, X-ray diffraction, and thermo-gravimetric analysis. Varied parameters such as methanol concentration in wash solvent, pH and salt concentration in elution solvent, elution volume, and mass ratio of sample to sorbent were optimized. The adsorption capacities of bisphenol A and diethylstilbestrol on the developed C18-modified halloysite were 6.3 and 14.2 mg g −1 , respectively, higher than those on the commercial C18 silica gel. Under the optimal condition, the average recoveries of bisphenol A and diethylstilbestrol by MSPD varied from 91.0 to 106.0%, and the relative standard deviations were less than 10.6% for human placenta samples. The limits of detection in the human placenta were 0.2 μg kg −1 for bisphenol A and diethylstilbestrol. The simple C18-modified halloysite-based MSPD method holds great potential for the determination of trace bisphenol A and diethylstilbestrol in the human placenta and other tissues of pregnant women with high sensitivity, accuracy, and reliability. Graphical abstract
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Qing Wang,Yao Long,Lin Yao,Li Xu,Zhi-Guo Shi,Lanying Xu
A mixed-mode chromatographic stationary phase , C18-DTT (dithiothreitol) silica (SiO 2 ) was prepared through “thiol-ene” click chemistry. The obtained material was characterized by fourier transform infrared spectroscope, nitrogen adsorption analysis and contact angle analysis. Chromatographic performance of the C18-DTT was systemically evaluated by studying the effect of acetonitrile content, pH, buffer concentration of the mobile phase and column temperature. It was demonstrated that the novel stationary phase possessed reversed phase liquid chromatography (RPLC)/hydrophilic interaction liquid chromatography (HILIC) mixed-mode property. The stop-flow test revealed that C18-DTT exhibited excellent compatibility with 100% aqueous mobile phase. Additionally, the stability and column-to-column reproducibility of the C18-DTT material were satisfactory, with relative standard deviations of retention factor of the tested analytes (verapamil, fenbufen , guanine , tetrandrine and nicotinic acid) in the range of 1.82–3.72% and 0.85–1.93%, respectively. Finally, the application of C18-DTT column was demonstrated in the separation of non-steroidal anti-inflammatory drugs, aromatic carboxylic acids , alkaloids, nucleo-analytes and polycyclic aromatic hydrocarbons . It had great resolving power in the analysis of various compounds in HILIC and RPLC chromatographic conditions and was a promising RPLC/HILIC mixed-mode stationary phase.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2016
·
IF 3.169
Qing Wang,Ling Tong,Lin Yao,Peng Zhang,Li Xu
In the present study, a mixed-mode stationary phase, C18-Diol, was applied for fingerprint analysis of traditional Chinese medicines. Hydrophobic, hydrogen bonding and electrostatic interactions were demonstrated to contribute the retention separately or jointly, which endowed the C18-Diol stationary phase with distinct selectivity compared to the bare C18 one. The separation of total alkaloids extracted from Fritillaria hupehensis was compared on the C18-Diol and conventional C18 column with the greater resolving power and better symmetry responses on the former one. Besides, a novel two-dimensional liquid chromatography on the single column (2D-LC-1C) was realized on C18-Diol with the offline mode for the alcohol extract of Fritillaria hupehensis and online mode for Ligusticum chuanxiong Hort. The early co-eluted extracted components with great polarity on the first dimension were reinjected on the same column and well separated on the second dimension. The results exhibited that the two complementary RPLC and HILIC modes on C18-Diol stationary phase enhanced the separation capacity and revealed more abundant chemical information of the sample, which was a powerful tool in analyzing complex herbal medicines.
研究领域:
分析化学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Yali Yang,Jia Chen,Xiaojing Liang,Bei Liu,Kaijun Quan,Xiuhui Liu,Hongdeng Qiu
In this paper, three imidazole- and C18- bifunctional silica stationary phases (Sil-Im-C18) were prepared by adjusting introduction interval of octadecyltrichlorosilane (ODS) and 3-imidazol-1-ylpropyl(trimethoxy)silane (TMPImS), which can be used for reversed-phase liquid chromatography (RPLC) and ion exchange chromatography (IEC) with adjustable performance. The successful preparation of Sil-Im-C18 were confirmed by the characterizations of elemental analysis, infrared spectroscopy (FTIR) and contact angle (CA). Chromatographic performance of Sil-Im-C18 were evaluated by the separation of Tanaka test mixture, alkylbenzenes, linear PAHs and a set of analytes with different properties (uracil, phenol, 1,2-dinitrobenzene and naphthalene), and compared with commonly used C18 column. It was found that the chromatographic performance of Sil-Im-C18 changed significantly with the difference in bonding amount of imidazole and C18. Sil-Im-C18 demonstrated the excellent separation performance towards polycyclic aromatic hydrocarbons (PAHs), phenylesters, phenylamines , phenols and inorganic anions, and notably, nucleobases and nucleosides can be separated using pure water as mobile phases . The van Deemter plot showed that the column efficiency of Sil-Im-C18-3 was 64,933 plate· m − 1 for naphthalene, indicated that Sil-Im-C18 was reasonably chromatographic columns. The RSD values of retention time were 0.22 %-0.61 % for 10 needles alkylbenzenes injected continuously at 50 °C to investigate thermal stability and repeatability, all the fluctuations of k of naphthalene were less than 2.3 % for Sil-Im-C18-1 during flushing 24 h with the mobile phase at different pH values (pH = 3 and 8), the retention time of alkylbenzenes were almost same for Sil-Im-C18-1 at different time, the RSD values of retention time of alkylbenzenes were 0.45 %-2.28 % for two batches Sil-Im-C18-1, revealing the excellent repeatability, thermal stability, durability and reproducibility of Sil-Im-C18, and implying a commercial prospect.
研究领域:
分析化学
·
色谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Shun Zhang,Ting Cai,Jing Lin,Jia-Juan Liu,Yong-Gang Zhao,Min-Yi Cao
Because of the “enterohepatic circulation” of bile acid, liver damage can be reflected by monitoring the content of bile acid in the serum of the organism. To monitor the concentration of 15 bile acids in plasma samples, a new technique of PRiME (process, ruggedness, improvement, matrix effect, ease of use) pass-through cleanup procedure combined with high performance liquid chromatography-tandem quadrupole mass spectrometry (HPLC-MS/MS) was developed. The sorbent used in the PRiME pass-through cleanup procedure is a new type of magnetic organic resin composite nano-material modified by C18 (C18-PS-DVB-GMA-Fe 3 O 4 ), which has high cleanup efficiency of plasma samples. It also shows good performance in the separation and analysis of 15 kinds of bile acids. Under the optimal conditions, the results show higher cleanup efficiency of C18-PS-DVB-GMA-Fe 3 O 4 with recoveries in the range of 82.1–115 %. The limit of quantitative (LOQs) of 15 bile acids were in the range of 0.033 µg/L-0.19 µg/L, and the RSD values of 15 bile acids were in the range of 3.00–11.9 %. Validation results on linearity, specificity, accuracy and precision, as well as on the application to analysis of 15 bile acids in 100 human plasma samples demonstrate the applicability to clinical studies.
研究领域:
分析化学
·
医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Junlang Qiu,Guosheng Chen,Shuqin Liu,Tianlang Zhang,Jiayi Wu,Fuxin Wang,Jianqiao Xu,Yan Liu,Fang Zhu,Gangfeng Ouyang
A novel solid-phase microextraction (SPME) fiber was prepared by gluing poly(diallyldimethylammonium chloride) (PDDA) assembled graphene oxide (GO)-coated C18 composite particles (C18@GO@PDDA) onto a quartz fiber with polyaniline (PANI). The fiber surface coating was sequentially modified with bioinspired polynorepinephrine, which provided a smooth biointerface and makes the coating suitable for in vivo sampling. The novel custom-made coating was used to extract acidic pharmaceuticals, and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) was employed for analysis. The custom-made coating exhibited a much higher extraction efficiency than the previously used commercial polydimethylsiloxane (PDMS) and polyacrylate (PA) coatings. The custom-made coating also possessed satisfactory stability (the relative standard deviations (RSDs) ranged from 1.60% to 10.3% for six sampling-desorption cycles), interfiber reproducibility (the RSDs ranged from 2.61% to 11.5%), and resistance to matrix effects. The custom-made fibers were used to monitor the presence of acid pharmaceuticals in dorsal-epaxial muscle of living fish, and satisfactory sensitivities (limits of detection ranged from 0.13 ng/g to 7.56 ng/g) were achieved. The accuracies were verified by the comparison with liquid extraction. Moreover, the novel fibers were successfully used to monitor the presence of acidic pharmaceuticals in living fish, which demonstrated that the custom-made fibers were feasible for possible long-term in vivo continuous pharmaceutical monitoring.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2024
·
IF 5.3
Qianqian Wang,Yuwei Liu,Yuanyuan Zheng,Di Chen,Ya Xie,Nian Shi
This work presents an advanced automated monolithic C18 pipette-tip solid-phase extraction (PT-SPE) method seamlessly integrated with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for precise therapeutic drug monitoring of eleven tyrosine kinase inhibitors (TKIs) in biological samples. Commercially available MonoTip C18 columns facilitate superior extraction efficiency and selectivity from complex biological matrices owing to their large surface areas and enhanced mass transfer capabilities. The sample preparation process, conducted by aspirating and dispensing solutions using a pipettor, completes the overall extraction process within 3 min. The method validation demonstrated excellent linearity within the range of 0.2/0.5/0.8 to 200 ng/mL, with detection limits ranging from 0.049 to 0.206 ng/mL. The method exhibited outstanding accuracy and precision, with intraday relative recovery rates ranging from 96.3 % to 115.0 % and interday recovery rates ranging from 95.0 % to 115.7 %, with coefficient of variation values consistently below 13.8 %. The proposed method is distinguished by its exceptional efficiency, reduced solvent use, enhanced environmental sustainability, and streamlined automation—key advantages in the realm of clinical therapeutic drug monitoring of TKIs. Overall, the MonoTip C18 PT-SPE technique, coupled with LC-MS/MS, offers a formidable, eco-friendly, and effective strategy for the TDM of TKIs, marking a significant advancement in the field.
研究领域:
分析化学
·
临床医学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Likai Qi,Xinyi Jiang,Ning Wu,Jingjing Li,Bin Di,Fang Yan
In this study, a novel polydopamine and C 18 dual-functionalized magnetic core-shell mesoporous silica (Fe 3 O 4 @nSiO 2 @mSiO 2 @PDA-C 18 ) nanocomposite was designed and synthesized, which was employed as an adsorbent to extract illegal drugs from urine samples by magnetic solid-phase extraction (MSPE) procedures. The as-prepared nanocomposite was fully characterized and combined with ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to monitor 12 trace illegal drugs and metabolites, including 6-monoacetylmorphine (6-MAM), morphine (MOR), codeine (COD), amphetamine (AMP), methamphetamine (MAMP), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyamphetamine (MDA), ketamine (KET), norketamine (NK), cocaine (COC), benzoylecgonine (BZE) and methcathinone (MC). Optimal MSPE procedures were achieved by fully investigating parameters of activation, adsorption, washing and desorption steps. The MSPE-UHPLC-MS/MS method offered high sensitivity with limit of detection (LOD) range of 0.005–0.05 ng mL -1 and good linearity with the concentration of 0.01–1000 ng mL -1 . Also, the intra- and inter-day recovery respectively ranged in 90.2–105.0% and 89.8–107.4%, and the intra- and inter-day precision was in the range of 0.5–14.0% and 1.2–10.0%, respectively. By application to real urine samples, the proposed method could not only be equally sensitive for quantifying 12 illegal drugs in urine but also significantly reduce the pretreatment time when comparing with methods based on solid-phase extraction. Furthermore, the designed dual-functionalized adsorbents could also be applied for on-site determination of MAMP in urine coupled with urine test kit, and the detection threshold of MAMP urine test kit remarkably reduced from 1000 ng mL -1 to 50 ng mL -1 . Therefore, the present method was a convenient, economic and sensitive approach for determining illegal drugs in urine samples both on site and in the laboratory.
研究领域:
分析化学
·
法医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·
IF 4.2
Chao Lei,Shun Zhang,Wen-Xin Liu,Ming-Li Ye,Yong-Gang Zhao
A novel magnetic nanomaterial with Fe 3 O 4 as the core, PS-DVB as the shell layer, and the surface modified with C18 (C18−PS−DVB−Fe 3 O 4 ) had been synthesized by seeded emulsion polymerization. C18−PS−DVB−Fe 3 O 4 retains the advantages of the chemical stability, large porosity, and uniform morphology of organic polymers and has the magnetic properties of Fe 3 O 4 . A simple, flexible, and efficient magnetic dispersive solid phase extraction (Mag-dSPE) method for the extraction of preservatives, sweeteners, and colorants in river water was established. C18−PS−DVB−Fe 3 O 4 was used as an adsorbent for Mag-dSPE and was coupled with high-performance liquid chromatography (HPLC) to detect 11 food additives: acesulfame, amaranth, benzoic acid, tartrazine, saccharin sodium, sorbic acid, dehydroacetic acid, sunset yellow, allura red, brilliant blue, and erythrosine. Under the optimum extraction conditions, combined with ChromCoreTMAQC18 (5 μm, 4.6 × 250 mm), 20 mmol/L ammonium acetate aqueous solution and methanol were used as mobile phases, and the detection wavelengths were 240 nm and 410 nm. The limits of detection (LODs) of 11 food additives were 0.6–3.1 μg/L with satisfactory recoveries ranging from 86.53% to 106.32%. And the material could be reused for five cycles without much sacrifice of extraction efficiency. The proposed method has been used to determine food additives in river water samples, and results demonstrate the applicability of the proposed C18−PS−DVB−Fe 3 O 4 Mag-dSPE coupled with the HPLC method to environment monitoring analysis.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Ruiping Wei,Mingzhu Yao,Ziqi Wang,Huijun Liu,Rongying Xia,Lei Yang,Guanghui Ma,Lijing Gao,Xiaomei Pan,Guomin Xiao
The properties of support have significant effects on the catalytic performance of supported catalysts. The present study involves the synthesis of a series of Mo-modified NbOPO4 supports, which were subsequently utilized for the preparation of supported NiCu bimetallic catalysts via a hydrothermal method. All catalysts were evaluated in the HDO of oleic acid to long-chain alkanes without carbon loss, an essential procedure for contemporary industrial applications. The effects of acidity and porosity on the dispersity and valence states of the metal components were systematically investigated via BET, XRD, SEM, XPS, NH3-TPD, and H2-TPR. Catalytic performance of the materials for HDO of oleic acid was assessed and it revealed that NiCu supported on Mo-modified NbOPO4 exhibited significantly enhanced selectivity for C18 compared to those supported on NbOPO4 (53.10% VS 84.16%) under the mild reaction condition of 250 °C reaction temperature, 3 MPa initial H2 pressure and 7 h reaction time. The resluts demonstrated the significant potential of Mo-modified NbOPO4 as a support for NiCu bimetallic catalysts, enabling efficient conversion of oleic acid into C18 and highlighted the crucial role of support modification in enhancing catalytic performance.
研究领域:
催化化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Bo Dai,Jiale Liang,Tiantian Xiao,Lei Jiang,Shujuan Ma,Xi Chen,Junjie Ou
Development of cholesterol-functionalized core-shell silica microspheres for reversed-phase liquid chromatography. • Significant reduction in analysis time and improved selectivity for polar compounds compared to conventional C18 stationary phases. • Successful application in complex sample analysis of sweeteners with LC-MS demonstrated an enhanced separation performance. To address the limited selectivity of C18 stationary phase toward structurally similar and polar compounds in complex matrices, we developed a cholesterol-functionalized core-shell silica stationary phase (SiO 2 @SiO 2 -chol). Monodisperse core–shell silica microspheres were fabricated via a two-phase method, followed by surface modification using silane coupling to immobilize cholesterol ligands. For direct comparison, a C18-bonded core-shell material (SiO 2 @SiO 2 -C18) was prepared under identical conditions. The SiO 2 @SiO 2 -chol stationary phase could not only reduce analysis time for alkylbenzene separations, but also increase resolution for structurally similar compounds such as PAHs, compared to the SiO 2 @SiO 2 -C18. This improvement is attributed to multimodal interactions, including hydrophobic effects, hydrogen bonding and steric hindrance, which are particularly effective for resolving polar and structurally similar analytes. For instance, naphthalene was used as a probe, showing a column efficiency (N) of 131,760 N·m −1 on SiO 2 @SiO 2 -chol, nearly double that of the SiO 2 @SiO 2 -C18 (70,470 N·m −1 ). In the separation of active ingredients from traditional Chinese medicines (TCMs), the SiO 2 @SiO 2 -chol column offered baseline separation of four target analytes (quercetin, apigenin, methoxsalen, and curcumin), whereas satisfactory resolution could not be achieved using the C18 stationary phase. Furthermore, the SiO 2 @SiO 2 -chol column was successfully coupled with LC-MS for the quantification of sweeteners in complex matrices, demonstrating practical applicability for food safety analysis. Mechanistically, the cholesterol moiety introduces additional shape-selective interactions and steric hindrance, which are absent in C18 materials, thereby enhancing the retention and separation of analytes with subtle structural differences. Taken together, the cholesterol-functionalized core-shell stationary phase combines structural robustness with multimodal recognition, providing a versatile and efficient alternative for separation of various analytes. Download: Download high-res image (82KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）