OPTICAL FIBER TECHNOLOGY
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2025
·
IF 2.7
Ling Chen,Ruike Liu,Xianfeng Liu,Zhaoyang Li,Guohao Li
A highly economical fiber immunosensor with the harmonic Vernier effect (HVE) is reported for cancer antigen 125 (CA125) tumor marker detection. The sensor architecture is constructed using a special fiber fusion splicer to cascade three sections of single-mode fiber (SMF) in sequence with a large offset. This configuration yields a cascaded Fabry-Perot interferometer (FPI) architecture incorporating the HVE. The misalignment fusion design facilitates direct and seamless interaction between biochemical fluids and optical signals. Experimental results demonstrate that the refractive index sensitivities of the internal envelope and upper envelope reach −5236, and 1163 nm/RIU, respectively, when refractive index increases from 1.334 to 1.339. It has excellent consistency with the simulation results of −5375 nm/RIU and 1257 nm/RIU as refractive index increases from 1.334 to 1.335. Obviously, the refractive index sensitivity is significantly enhanced via the HVE. The designed sensor is further biologically modified with the 50 μg/mL ovarian cancer 125 monoclonal antibody, enabling varying cancer antigen 125 concentrations detection. The proposed biosensor has low detection limit with value of 44.12 fg/mL and a fast response time (<30 min). Therefore, the proposed biosensor has latent application as an ideal platform for disease diagnosis and medical detection.
研究领域:
生物传感器
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生物光子学
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癌症诊断
·
光学传感
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光纤技术
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生物医学工程
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免疫传感器
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Kang Zou,Yamin Fu,Ruiying Yang,Xiaohua Zhang,Cuicui Du,Jinhua Chen
By direct calcination of Cu 2+ -doped ZIF-8 in oxygen atmosphere, a new photoelectric material, CuO–ZnO heterojunction , was prepared. The morphology and structure were investigated in detail by several methods, such as scanning electron microscopy, transmission electron microscopy , nitrogen adsorption-desorption method and X-ray diffraction. Under visible light irradiation , CuO–ZnO heterojunction exhibits excellent PEC behavior in aqueous media with the presence of ascorbic acid (AA) and dissolved oxygen at −0.3 V. Taking the CA125-antibody functionalized CuO–ZnO heterojunction (CuO–ZnO-Ab 2 ) as a signal source, a novel photoelectrochemical (PEC) immunosensing platform was developed for ultrasensitive assay of CA125. Because of the target-induced introduction of photoelectric material to the sensing platform, the proposed PEC immunosensor has excellent performance for CA125 assay: near-zero background noise, wide linear response range (1 × 10 −5 U/mL to 100 U/mL) and low detection limit (3.16 × 10 −6 U/mL). The developed CuO–ZnO heterojunction and the related PEC sensing platform may have promising applications in ultrasensitive and highly accurate monitoring of various proteins in bioanalysis and disease diagnosis.
研究领域:
生物分析
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Zheng Chang,Qiqi Zhao
In this paper, using a strong coordination of the multi-amino groups of polyethylenimine (PEI)/SiO 2 nanoparticles with Cu 2+ and the weaker carboxyl-amine interactions with heme chloride (hemin), novel Cu 2+ -hemin dual-functionalized PEI/SiO 2 nanoparticles (abbreviated as Cu 2+ /hemin/PEI/SiO 2 NPs) were conveniently synthesized. The Cu 2+ /hemin/PEI/SiO 2 NPs exhibit both the peroxidase-like activity of hemin and the electrochemical signaling properties of Cu 2+ . Moreover, a large number of hemin molecules were successfully loaded onto Cu 2+ /PEI/SiO 2 NPs through electrostatic interactions. This not only prevented their self-aggregation-induced inactivation in aqueous solutions, but also catalytically amplified the electrooxidation signal of Cu 2+ /PEI/SiO 2 NPs by shortening the electron transfer distance. Subsequently, these nanoparticles spontaneously assembled on the overoxidized polypyrrole-modified reduced graphene oxide (abbreviated as OPPy/rGO) electrode surface through Cu N coordination between OPPy and Cu 2+ , and π-π stacking interactions between hemin and rGO. As a novel immunosensing platform, a label-free electrochemical immunosensor for carbohydrate antigen-125 (CA125), a specific biomarker for epithelial ovarian cancer was developed by covalently immobilizing CA125 capture antibodies onto the Cu 2+ /hemin/PEI/SiO 2 NPs@OPPy/rGO hybrid nanomaterials. Elevated serum CA125 levels correlate significantly with ovarian cancer progression. With the increasing concentration of CA125 antigen, the current responses of this immunosensor were decreased due to the formation of antigen-antibody insulating immune complexes. The proposed immunosensor exhibited excellent performances within a wide linear range from 5 × 10 −5 to 200 U·mL −1 for CA125 and a low detection limit of 1.2 × 10 −5 U·mL −1 (S/N = 3). Additionally, this immunosensor demonstrated good stability, reproducibility, and selectivity, making it a promising tool in clinical screening, early diagnosis, and prognostic monitoring of ovarian cancer.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物电化学
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癌症诊断
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材料科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Min Chen,Rui Han,Wenqi Wang,Yang Li,Xiliang Luo
A brief and universal ultralow fouling sensing platform capable of assaying targets in complex biofluids was developed based on designed antifouling peptides that could form a loop-closed structure with enhanced stability. The newly designed peptide with thiol groups in its two terminals self-assembled onto a gold nanoparticle (AuNP)-modified electrode surface to form a stable loop structure, which displayed excellent antifouling performance, outstanding stability under enzymatic hydrolysis, and satisfactory long-term antifouling capability in complex biofluids (clinical human serum). The antifouling and highly sensitive electrochemical aptasensor was constructed via one-step co-immobilization of the designed peptides and aptamers onto the electrode surface modified with electrodeposited poly(3,4-ethylenedioxythiophene) (PEDOT) and AuNPs. The developed peptide-based aptasensor exhibited a decent response for the analysis of the cancer antigen 125 (CA125), with a relatively wide linear range (0.1–1000 U mL–1) and a low limit of detection (0.027 U mL–1), and was capable of detecting CA125 in clinical serum samples with acceptable accuracy. This antifouling strategy-based self-assembled peptide with a loop-closed structure provided a potential path for the development of various low-fouling biosensors for application in complex biological fluids.
研究领域:
生物传感器
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电化学
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纳米技术
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分析化学
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生物医学工程
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Meiqing Yang,Lu Wang,Congkai Xie,Haozi Lu,Junhua Wang,Ye Li,Huimin Li,Jifei Yang,Tingting Zhang,Song Liu
Cancer antigen 125 (CA125) is the gold standard biomarker for clinical diagnosis of ovarian cancer, with a threshold value of 35 U/mL in serum. In this paper, a disposable ultrasensitive immunosensor based on Ti 3 C 2 T x -MXene/amino-functionalized carbon nanotube (NH 2 -CNT) modified screen-printed carbon electrode (SPCE) was constructed for the detection of the ovarian cancer antigen CA125. By optimizing the mass ratio of Ti 3 C 2 T x to NH 2 -CNT, Ti 3 C 2 T x /NH 2 -CNT composite with excellent electrochemical properties was prepared, which is beneficial for amplifying the initial electrochemical signal. The positively charged NH 2 -CNT effectively alleviated the stacking problem of Ti 3 C 2 T x , and its amino group also facilitated the covalent immobilization of the capture antibody. Meanwhile, chitosan (CS) with excellent film-forming ability was also used to successfully enhance the adsorption of electrode material, thus improving the stability of the sensor. In addition, CS could further enhance the current signal. The prepared immunosensor exhibited excellent performance in CA125 detection with a wide linear range from 1 mU/mL to 500 U/mL, and good selectivity, reproducibility and lomg-term stability. Furthermore, the immunosensor showed satisfactory results for the detection of CA125 in clinical serum samples, which is promising for the clinical screening, early diagnosis and prognostic examination of ovarian cancer.
研究领域:
生物传感器
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电化学
·
癌症诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2023
·
IF 4.2
Li He,Ciping Chen,Yongge Liu,Hong Hai,Jianping Li
A novel electrochemiluminescence(ECL) aptamer biosensor with high sensitivity and selectivity for the detection of tumor biomarker carbohydrate antigen CA125 was constructed, and a strategy of triple amplification of signals was proposed by using exonuclease cyclic cleavage aptamer, combined with rolling ring amplification technologies, generating multi-branched dendritic double-stranded DNA to load a large number of probes through "strand self-growth". The double-stranded DNA, which abbreviated as CP/CA dsDNA, formed by hybridizing the single strand of capture DNA (CP DNA) with the single strand DNA of the CA125 aptamer(CA Apt) was modified on the Fe3O4@Au. When the carbohydrate antigen CA125 was added, CP/CA dsDNA were unwound, and CA125 specifically combined with CA Apt to form a protein-aptamer complex, leaving only CP DNA on the surface of the Fe3O4@Au. RecJf exonuclease cleaved the aptamer in the protein–aptamer complex and released CA125, which recombined to other CA125 aptamers, to form a cycle that produces more CP DNA on the Fe3O4@Au. Three ssDNA (H1, H2, H3) were introduced and hybridized with CP DNA to form a dsDNA with "+" configuration structure. Then phi29 DNA polymerase, T4 DNA ligase, deoxy-ribonucleoside triphosphate (dNTPs) and padlock probes were added to form a large number of complementary strands of padlock probes (CS padlock probes) by rolling cyclic amplification. CS padlock probes were linked to the "+" type dsDNA; then the ssDNA H4 was added and hybridized with CS padlock probe to form multi-branched dendritic dsDNA. A large number of Tris(2,2'-bipyridyl)ruthenium(II) probes were embedded in the double strands, resulting in an extremely strong ECL signal in the presence of the co-reactant tri-n-propylamine (TPA). There is a linear relationship between the ECL signals and CA125 concentrations in the range of 1.0×10-15~1.0×10-8 mg/mL, and the detection limit was 2.38 × 10-16 mg/mL. It has been used for the determination of CA125 in serum samples.
研究领域:
生物传感器
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分析化学
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癌症标志物
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Zheng Chang,Qiqi Zhao,Binghua Yao,Lei Han
Novel electroactive nanotags of Cu(II) functionalized polyethylenimine (PEI)/SiO2 nanoparticles (abbreviated as Cu2+/PEI/SiO2 NPs) were conveniently synthesized using a strong coordination reaction between Cu(II) and the multi-amino groups of PEI. These electroactive nanotags were easily coated on the overoxidized polypyrrole (OPPy) modified Nafion/multi-walled carbon nanotubes (abbreviated as Nafion/MWNTs) electrode surface through Cu-N bonds and strong electrostatic interactions. The Cu2+/PEI/SiO2 NPs@OPPy/MWNTs hybrid nanomaterial was formed without the addition of extra membrane-forming substances. The experimental results demonstrate that the OPPy/MWNTs composite membrane coupled with electrocatalytic Cu(Tris)42+ complexes in tris(hydroxymethyl)aminomethane (Tris)-HCl buffer medium amplified the electrochemical signals of nanotags based on the mutual synergistic effects. As an application, a label-free electrochemical immunosensor for epithelial ovarian cancer biomarker-carbohydrate antigen-125 (CA125) was constructed by covalently immobilizing CA125 antibody onto the hybrid nanomaterial modified electrode. With the increasing concentrations of CA125 antigen, different decreases in the electrochemical signals of the nanotags were observed due to the formation of antigen-antibody immune complexes. The electrochemical immunosensor showed a linear relationship from 0.001 to 500 U·mL−1 for CA125 with a detection limit of 3.4 × 10−4 U·mL−1. In addition, the proposed immunosensor was applied to the determination of CA125 in human serum with satisfactory results.
研究领域:
生物化学
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纳米技术
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分析化学
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癌症生物学
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电化学
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Weiyuan Lyu,Xinrui Cheng,Ziqing Yu,Ruirui Dong,Zheyi Sheng,Ting Zhang,Xia Yin,Feng Shen
Ovarian cancer (OC) is one of the three major gynecologic malignancies and has the highest mortality rate because of the late diagnosis. Liquid biopsy based on serum protein biomarkers has demonstrated great potential for early diagnosis but remains limited by the analysis performance of conventional immunoassay technologies, such as chemiluminescence, and biomarkers, such as CA125. To address this challenge and achieve accurate early-stage diagnosis of OC, we developed a digital immunoassay on a SlipChip (DiSC) for quantitative analysis of a potential serum protein biomarker, Spondin-1 (SPON1). The DiSC system achieved a limit of detection (LoD) of 23 fg/μL for digital quantification of SPON1. The DiSC system was utilized to quantify the serum level of SPON1 in 357 clinical serum samples, including 63 from patients with benign ovarian tumors and 294 from patients with malignant ovarian cancer, ranging from stages I to IV. SPON1 concentrations were significantly different in samples from patients with malignant ovarian cancer. Notably, significantly different SPON1 levels were observed in early stages (I and II), in lymph node-negative cases (N 0 ), and before metastasis (M 0 ), suggesting that SPON1 could serve as a sensitive diagnostic biomarker for early-stage OC. The differential diagnostic model based on SPON1 levels quantified using DiSC demonstrated an area under the curve (AUC) of 0.8187 for early-stage OC, a significant improvement over CA-125 (AUC = 0.6967). For OC of all stages, the AUC was 0.8225, which could be further increased to 0.8750 when combined with CA-125. This showcases the potential of SPON1 as a novel biomarker for sensitive early-stage diagnosis of ovarian cancer and the capability of the DiSC system in discovering low-abundance biomarkers for disease diagnosis.
研究领域:
肿瘤学
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生物医学工程
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临床诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Zhe Qiao,Zilian Jiang,Qiufen Luo,Hongfang Zhang,Jianbin Zheng
Labeling with redox reporter is often required in developing electrochemical bioassay for most proteins or nucleic acid biomarkers. Herein, a label-free ratiometric immunosensing platform is firstly developed by integrating the antibody-conjugated nanochannels with a smart modified electrode . The electrode modifier is the composite of C 60 , tetraoctylammonium bromide (TOA + ) and Prussian blue (PB). Cyclic voltammograms of the ultimate C 60 -TOA + /PB modified electrode exhibited two pairs of peaks at 0.15 V and −0.13 V, ascribing to the redox of PB and C 60 , respectively. With the addition of K 3 [Fe(CN) 6 ] in the electrolyte solution , the peaks of PB decreased due to the adsorption of [Fe(CN) 6 ] 3− while the peaks of C 60 increased because of the formation of the ternary complex (TC) C 60 -TOA + -[Fe(CN) 6 ] 3− . As a result, the peak current ratio I PB / I TC decreased gradually with the increment of the concentration of [Fe(CN) 6 ] 3− . For the nanochannels-based immunosensing platform, the steric hindrance of the bioconjugated nanochannels varied with the loading amount of the target CA125, and thus [Fe(CN) 6 ] 3− passing through the channels was quantitatively affected. And the higher CA125 level was, the less [Fe(CN) 6 ] 3− concentration was. And thus, the ratio I PB / I TC monitored at the C 60 -TOA + /PB modified electrode increased with the increase of the concentration of CA125. The ratiometric immunoassay featured a linear calibration range from 1.0 U mL −1 to 100 U mL −1 with a low detection limit of 0.86 U mL −1 . In addition, the ratiometric immunosensing platform demonstrated good specificity and stability as well as acceptable accuracy in overcoming the effect of electrode passivation which was an inherent problem of electroanalysis.
研究领域:
生物传感器
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医学检测
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电化学分析
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2018
·Vol. 54
·期 4
·3331-3341
·
IF 2.993
Han Shuang,Su Liqiang,Zhai Minghui,Ma Lin,Liu Shiwei,Teng Yue
Conventional cancer chemotherapy presents relevant limitations associated with the non-selectivity of cytotoxic drugs, their narrow therapeutic indices and limited cellular penetration. Molecularly imprinted polymers (MIP) prepared using molecular imprinted technology has the advantages such as high stability against chemical and enzymatic attack, high selectivity for a specific template, and the capability of resistance to harsh environment. In this research, the MIP with cancer antigen 125 (CA125) tumor markers as template molecule, combined with molecular imprinting technique was prepared on the surface of graphene oxide for drug delivery. As a result the prepared composite had the properties of specific binding to CA125 and biocompatibility. Along with this, the composites could target specific tumor cell by the capture of CA125. CA125-molecularly imprinted polymers as a recognition element for the tumor cell could eliminate the use of antibody, enhance stability and prolong the retention time of drugs surrounding them. Ultimately, this method developed a cost-effective and stable way to target tumor cell for drug delivery.
研究领域:
生物医学工程
·
药学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Shiyuan Wang,Jiaqi Yang,Junyu Chen,Yasheng Dai,Changgan Lai,Tao Jiang,Yuning Pan
Dual-mode detection integrating SERS and UCL for sensitive and visualized detection of CA125. • A type-II band alignment for efficient charge transfer was featured via MXene/MoS 2 heterostructure. • The dual-mode immunoassay kit was developed as a practical tool for clinical ovarian cancer diagnostics. Ovarian cancer (OC) is one of the most common cancers with a high mortality rate, posing a severe threat to female health. Cancer antigen 125 (CA125) has been broadly acknowledged as a significant oncomarker for monitoring OC. Herein, we present a dual-mode immunosensor that integrates surface-enhanced Raman scattering (SERS) and upconversion luminescence (UCL) to facilitate the sensitive and visualized detection of CA125. The MXene/MoS 2 composites were employed as the SERS substrate, whose abundant surface functional groups enable robust antibody immobilization while efficient interfacial charge transfer markedly boost the chemical enhancement. Specifically, a typical type-II band alignment was observed in MXene/MoS 2 heterostructure, which was favorable for efficient charge transfer and resulted in a significant SERS effect with an enhancement factor of 5.24 × 10 7 . Furthermore, the co-doped Yb 3+ /Er 3+ ions UCL probes were employed to improve the detection capability for CA125 by visible green fluorescence. The developed dual-mode immunosensor demonstrated a wide linear detection response, covering the range from 10 to 10 −4 IU/mL. Moreover, the immunosensor displayed a highly sensitivity in detecting of CA125, with a limit of detection as 4.46 × 10 −5 IU/mL for SERS mode and 5.01 × 10 −5 IU/mL for UCL mode. Based on the favorable dual-mode immunosensor, it is proposed as a reliable tool that shows great potential for the detection of CA125. Download: Download high-res image (334KB) Download: Download full-size image
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DOI
高影响因子
IEEE TRANSACTIONS ON ELECTRON DEVICES
·
2025
·
IF 3.2
Yao Li,Yuying Zhou,Bing Chen,Shuaidi Zhang,Ke Hu,Peiyun Li,Congyan Lu,Jiawei Wang,Xiaonan Yang,Feng Zhang,Wenchang Zhang,Ling Li
This work proposes a dual-gate InGaZnO thin-film transistor sensor array for label-free and cross-scale multiple biomarkers detection, including single-stranded deoxyribonucleic acid (ssDNA) and protein CA125. We exploit dual gate modulation for threshold voltage and CMOS-based SAR-assisted digital-to-analog converter (DAC) VBG calibration circuit to eliminate initial VTH offset variation caused by different functionalizations, which improve the reliability of the multibiomarker sensor array. At the same time, receptor enhancement on the indium gallium zinc oxide (IGZO) sensing membrane was detected by fluorescence assay, realizing higher sensitivity for biosensor. We demonstrate the sensor array response for pH in static and real-time mode, realizing near-Nernst limit sensitivity (55.02 mV/pH). Furthermore, at the top-gate configuration, with low operation voltage (0.1 V) and low offset variation (about 2 nA), the IGZO sensor array exhibits great performance for biomarker sensing which achieves sensitivity up to 4.426%/decade for ssDNA and 1102 nA/decade for CA125, with the limit of detection (LoD) of 1.67 aM for ssDNA and 0.1 fg/mL for CA125. This biosensor array provides a potential approach to realize miniaturization and high-density integration of multidimensional-biomarker detection.
研究领域:
生物医学工程
·
传感器技术
·
分子生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）