JOURNAL OF ALLOYS AND COMPOUNDS
·
2022
·
IF 6.371
Haoxiang Zhong,Lingzhi Zhang
The low sulfur utilization and the serious shuttle effect of polysulfides severely hamper practical application of Li-S batteries. Herein, sulfur cathodes with the multi-functional structures, featured with double-layered hollow carbon sphere with up to 50 nm interlayer space (DLHC-50) in situ grown Co-SnS catalytic layer is designed and synthesized for lithium sulfur batteries. Co-SnS/DLHC-50 can encapsulate 72% sulfur, higher than 63% for Co-SnS/DLHC-20 due to the large interlayer spaces of DLHC-50. Meanwhile, polysulfides are also effectively restricted via physical entrapment from DLHC-50 as well as chemical binding between Co-SnS and polysulfides. Benefiting from the well-constructed host framework, Co-SnS/DLHC-50 @S shows higher specific capacity/capacity retention of 760.4 mA h g −1 /72.9% after 500 cycles at 0.5 C, than that of 546.5 mA h g −1 /62.2% and 668.9 mA h g −1 /62.2%, for DLHC-50 @S, and Co-SnS/DLHC-20 @S, respectively. In addition, the strategy can be also extended to prepare other metal oxides (sulfides)/DLHC-50 for wide applications. Therefore, this work demonstrates a promising route to combat some bottlenecks such as low sulfur utilization, and the shuttle effect of polysulfides for Li-S batteries.
研究领域:
材料科学
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能源存储
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产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2618
·
IF 4.927
Jiao Zhou,Xinyue Lin,Shuangshuang Liu,Zhanbin Wang,Dongchao Liu,Yonghong Huo,Dehai Li
Inonotus hispidushas various health-promoting activities, such as anticancer effects and immune-stimulating activity. The commercialization of valuable plant triterpenoids faces major challenges, including low abundance in natural hosts and costly downstream purification procedures. In this work, orthogonal design was used to compound methyl jasmonate (MeJA), salicylic acid (SA), oleic acid, and Cu2+, and the effects of combinations on the total triterpenes biosynthesized were studied. The optimal combination was screened out and its effect on the activity of PAL, CAT, and SOD was studied. The optimal concentration of oleic acid was 2% when MeJA was 100 mol/L, and the total triterpenoid content and mycelia production were 3.918 g and 85.17 mg/g, respectively. MeJA treatment induced oxidative stress, and at the same time increased the activity of related defense enzymes. Oleic acid is thought to regulate cell permeability by recombining cell membranes. It promotes the material exchange process between cells and the environment without affecting cell growth. When oleic acid was used in combination with MeJA, a synergistic effect on triterpene production was observed. In conclusion, our findings provide a strategy for triterpenoid enrichment ofI. hispidus.Keywords:Inonotus hispidus;combination of elicitors;defense enzyme;triterpenoid
研究领域:
药理学
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真菌学
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生物化学
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天然产物化学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
·
IF 4.872
Shi-Long Ruan,Lei Xie,Jun-Wei Ou,Xue-Song Sun,Yong-Pu Zhang,Jian-Rao Hu
Cadmium (Cd) is an environmental toxicant and a nonessential metal. Cd can attack a wide range of organs, such as the liver, kidney, lung, ovary, testis, brain, and muscle in vertebrates. Among these organs, the testis might be the most sensitive organ to Cd toxicity. Metallothionein (MT) is a cysteine-rich protein with a low molecular weight, that can bind with Cd and eliminate reactive oxygen species (ROSs). Hydrogen peroxide, which as a crucial type of ROS that is induced by Cd, can be eliminated by catalase (CAT) in the self-protection of cells and to realize Cd toxicity resistance. To investigate the functions of MT and CAT in the testis of Cynops orientalis , we cloned the full-length MT and CAT genes of C. orientalis for the first time. Immunofluorescence results demonstrated that MT and CAT were expressed in Sertoli cells and all spermatogenic cells in the testis of C. orientalis . The results of the ultrastructural damage assay demonstrated that there were various impairments, which included organelle vacuolization, abnormal chromatin distribution, and apoptotic bodies, in somatic cells that were exposed to Cd. However, the anomalies of spermatozoa were located mainly in the mid-piece and head, many of which showed severely impaired structures. The results demonstrated that MT and CAT expression had distinct patterns in response to various Cd concentrations: an increase in MT mRNA levels with elevated Cd levels and a persistent increase in CAT mRNA levels with elevated Cd levels. These results suggested that MT and CAT play roles in Cd toxicity resistance in the testis and that the expression of CAT may be a better biomarker than the expression of MT for assessing Cd pollution.
研究领域:
环境毒理学
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分子生物学
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生殖生物学
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Si-Qi Wu,Yan-Xia Wang,Trust Beta,Su-Yan Wang,Gerardo Mendez-Zamora,Pedro Laborda,Daniela D. Herrera-Balandrano
Sprouting can increase the nutritional quality of seeds, such as soybeans. The current study aimed to evaluate the influence of exogenous melatonin (MLT; 100 and 300 μmol/L) on the isoflavone content and antioxidant properties of two soybean cultivars’ germination process (5, 6, and 7 days). MLT treatments significantly increased the sprout length and germination rate by 53% and 12%, respectively, when compared to water-treated soybean sprouts. Further, seven main isoflavones were quantified, with the highest total isoflavone content (5.101 g/kg) found on day 5 when using 100 μmol/L MLT. Additionally, when applying the same concentration, the highest levels of SOD and CAT were found, with values of 10.05 and 15.10 μkat/g, respectively, therefore resulting in a decrease in malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), and nitric oxide (NO) contents. MLT enhanced the expression levels of several genes related to antioxidant properties, seed germination, and root length. Overall, MLT is a suitable inducer for promoting soybean germination and enhancing the isoflavone content and antioxidant properties of soybean sprouts.
研究领域:
植物科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
Frontiers in Nutrition
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2022
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IF 6.59
Tao Liang,Gu Fan,Liu Yan,Yang Min,Wu Xing-Zhong,Sheng Jun,Tian Yang
Moringa oleifera leaves are a kind of new food raw materials, rich in functional factors, M. oleifera leaves aqueous extract have antioxidant activity and M. oleifera leave protein is an important active ingredient in the aqueous extract. Numerous studies have shown that peptides have strong antioxidant activity. To reveal the antioxidant effects of M. oleifera (MO) leaves peptides, MO leave antioxidant peptides were isolated and prepared to clarify their antioxidant activity. MLPH1 (<1 kDa), MLPH3 (1~3 kDa), MLPH5 (3~5 kDa), and MLPH10 (5~10 kDa) fractions were obtained by the membrane ultrafiltration classification of MO leaves proteolytic hydrolysate (MLPH). MLPH1 was further separated by centrifugal filters, and the fraction separated by <1 kDa (MLPH1-1) was identified and analyzed by LC–MS/MS. The purpose of this study was to investigate the effect of MO leaves antioxidant peptide pretreatment on H2O2-treated HepG2 cells and to refine the antioxidant activity. The results showed that MLPH1 had the strongest antioxidant activity, and three MO leaves antioxidant peptides (LALPVYN, LHIAALVFQ, and FHEEDDAKLF) were obtained. The peptide with the sequence LALPVYN and a molecular weight of 788.44 Da had the strongest antioxidant activity. After 24 h of LALPVYN pretreatment, the cell viability and the CAT, GSH-Px, and SOD enzyme activity were significantly increased, and the MDA, ROS, and apoptosis rates were significantly decreased. These results provide a theoretical basis for further research on the antioxidant mechanism of MO leaves peptides.
研究领域:
生物化学
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药理学
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细胞生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Xingchen He,Feixia Peng,Yihan Ma,Hongyu Wang,Xiaoyang Mo,Yani Ma,Xin Tang,Qian Li,Xueqin Ding
Wild animals, as a vital component of our natural world, serve a crucial role in preserving ecological equilibrium and biodiversity. By delving into the genetic constitution of wild animal populations, the evolutionary history, genetic diversity, and adaptation mechanisms could be explored, thereby informing conservation strategies and safeguarding the future of these species. In order to study the genetic information of wild animals, it is necessary to extract high purity and high concentration of wild animal genomic DNA. In this work, a hydrophobic magnetic deep eutectic solvent (HMDES) based vortexed extraction was developed for the extraction of genomic DNA from leopard cat ( Prionailurus bengalensis ), hairy-crowned deer ( Elaphodus cephalophus ) and muntjac ( Muntiacus reevesi ) muscle tissue, respectively. Extraction conditions like the pH value, extraction time, temperature and the amount of HMDES used were optimized by single-factor experiments. Under the optimized condition, genomic DNA could be selectively extracted from the three animal tissues. The limits of detection (LOD) and limits of quantification (LOQ) of the proposed method were 2.86 ng/μL and 8.66 ng/μL, respectively. Meanwhile, the relative standard deviation (RSD) of the method precision and repeatability were 1.64 % and 5.57 % at 20 ng/μL, showing the method has good precision and repeatability. After extraction, the DNA extracted into the HMDES droplets can be quickly recovered and the HMDES can be recycled and reused. The method proposed is a fast, environmental-friendly and high efficient extraction strategy for purification and enrichment of genomic DNA from leopard cat, hairy-crowned deer and muntjac tissues.
研究领域:
遗传学
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生物化学
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保护生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Nature Biomedical Engineering
·
2018
·Vol. 2
·期 8
·611-621
·
IF 0
Chao Yu,Xu Ligeng,Liang Chao,Feng Liangzhu,Xu Jun,Dong Ziliang,Tian Longlong,Yi Xuan,Yang Kai,Liu Zhuang
Radiation therapy for cancer can lead to off-target toxicity and can be ineffective against hypoxic solid tumours and distant metastases. Here, we show that intratumoral injection, in mouse and rabbit xenografts and in patient-derived mouse xenografts, of a sodium alginate formulation containing catalase (Cat) labelled with the therapeutic 131 I radioisotope enables long-term relief of tumour hypoxia and complete tumour elimination at low radioactivity doses. On injection, the soluble polysaccharide rapidly transforms into a hydrogel in the presence of endogenous Ca 2+ , fixing 131 I-Cat within the tumours. We also show that local radiotherapy with a formulation that includes the immunostimulatory CpG oligonucleotide combined with systemic checkpoint-blockade therapy using an anti-CTLA-4 antibody leads to metastasis inhibition and protection against tumour rechallenge. The local therapy, which uses only biocompatible components, might enable new strategies for local tumour treatments that can be combined with systemic therapeutic responses, for the inhibition of tumour metastasis and the prevention of tumour recurrence in patients with advanced-stage cancer.
研究领域:
肿瘤学
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免疫学
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放射医学
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产品关联
DOI
PubMed
JOURNAL OF HAZARDOUS MATERIALS
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2022
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IF 14.224
Wenqi Diao,Jin Yan,Xuedong Wang,Qiuhui Qian,Huili Wang
Although triclosan (TCS) is ubiquitously detected in environmental media and organisms, little information is available on its cardiotoxicity and underlying mechanisms. Herein, acute TCS exposure (0.69–1.73 μM) to zebrafish from embryos (6 hpf) to larvae (72 hpf) resulted in cardiac development defects, including increased angle between atrium and ventricle, prolonged SV-BA distance, linearized heart and pericardial cyst in 72-hpf larvae. These malformations resulted from interfered oxidative-stress pathways, reflecting in accumulated ROS and MDA and inhibited SOD and CAT activities. By RT-qPCR, the transcription levels of four cardiac development-related marker genes were significantly up-regulated except for gata4 . Besides, miR-144 was identified as a regulatory molecule of TCS-induced cardiac defects by integrating analyses of artificial intervene expression and RNA-Seq data. Interestingly, the target genes of miR-144 were found and interacted with the above marker genes through constructing protein-protein interaction networks. After intervening the expression of miR-144 by microinjecting and activating Wnt pathway by an agonist BML-284, we confirmed that up-regulated miR-144 suppressed the expression of angiogenesis-related genes and negatively regulated Wnt pathway, further triggering angiogenesis disorders and cardiac phenotypic malformation. These findings unravel the underlying molecular mechanisms regarding TCS-induced cardiac development toxicity, and contribute to early warning and risk management of TCS.
研究领域:
毒理学
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分子生物学
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发育生物学
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环境科学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Ting Zhou,Bao Yang
Prenylated resveratrols are drug candidates presented in many natural sources. They possess multiple pharmacological activities. Due to the limited abundance in nature and disadvantage of chemical synthesis, it is required to produce prenylated resveratrols by biological strategy. In this work, a prenyltransferase iacE from Pestalotiopsis fici was prepared by heterologous overexpression. It could catalyze 2- C -prenylation of resveratrol efficiently. Dimethylallyl diphosphate (DMAPP) and geranyl diphosphate (GPP) could serve as prenyl donor. Glu97, Thr114 and Tyr413 were proved to be important residues in the active site by site-directed mutagenesis. The product was purified and identified to be 2- C -prenyl resveratrol by NMR . 2- C -prenyl resveratrol exerted neuroprotective activity through inhibiting ROS overproduction and improving the activities of antioxidant enzymes in HT22 cell. The genes involved in antioxidant defense were regulated, including upregulated CAT , SOD2 , NFE2L2 , GPx4 , and downregulated PTGS2 and ACSL4 . These results supplied a novel technique for prenylated resveratrols production.
研究领域:
生物技术
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药理学
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神经科学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
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2022
·
IF 8.943
Giridhar Babu Anam,Dinneswara Reddy Guda,Young-Ho Ahn
A study was conducted to determine how melatonin (MLT), a growth regulator, affects Microcystis aeruginosa cell behaviour and how MLT exposed cells respond to hydrogen peroxide (H 2 O 2 ) treatment. MLT promotes the growth, chl- a content, Fv/Fm values, and microcystins (MCs) production of M. aeruginosa at low concentrations of 1–2.5 μmol/L but suppresses the growth at high concentrations (5–10 μmol/L). The cellular and genetic responses of MLT pre-treated cells to H 2 O 2 treatment were examined further. Further research found that the cells pre-treated with MLT were susceptible to a range of growth-promoting, inhibiting and lethal effects when exposed to higher levels of H 2 O 2 . A dose-dependent pattern was observed under conditions of 0.05–0.2 mmol/L H 2 O 2 with 0.5–2.5 μmol/L MLT concentrations to different degrees. High doses of H 2 O 2 (0.2 and 0.3 mmol/L) typically lead to cell lysis and release of MCs in 5.0 and 10 μmol/L MLT pre-treated cells. A decrease in SOD/CAT activities and an increase in MDA levels validated the growth reduction. Furthermore, higher cell lysis and release of intracellular MCs were observed when H 2 O 2 was increased for 5–10 μmol/L MLT pre-treated cells. This led to a higher accumulation of extracellular MCs. The results provide insight into how MLT influences H 2 O 2 damage and assist in identifying situations where H 2 O 2 treatment of cyanobacterial blooms is most appropriate.
研究领域:
环境科学
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毒理学
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水生生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2023
·
IF 7.5
Zhihong Deng,Lifeng Zhao,Xiuxue Li,Chang-Yun Wang,Yue Zhou,Mengfei Li,Yingfei Li,Xianjun Fu
Appropriate topical dressings for burn treatments are important to accelerate skin wound recovery and prevent external infections. This study aimed to evaluate the effect and investigate the mechanism of folium crataegi ( Crataegus pinnatifida Bge. ) for the treatment of burn wounds, as well as to compare the therapeutic effects of aqueous extracts ( HLW ) and alcoholic extracts ( HLE ) from folium crataegi . The results demonstrated that both HLW and HLE groups exhibited a higher wound contraction rate than the silver sulfadiazine ( SSD ) ointment group. Moreover, HLW showed more significant wound repair effects than HLE . HLW significantly increased levels of EGF and FGF-2 in wound tissue, as well as TGF-β1 , VEGF , CAT and IL-10 in serum. Folium crataegi extract, especially aqueous extracts, exerted good anti-inflammatory, anti-oxidant and anti-bacterial effects by upregulating the expression of lag3 , txn1 and slpi , respectively. Folium crataegi extract significantly inhibits the expression of npas2 , a key gene in the circadian rhythm pathway. In conclusion, this research illustrated that the folium crataegi extract, especially aqueous extracts, had better therapeutic effects on skin burns through multiple ways, possibly including a novel mechanism related to circadian rhythm pathway. These findings suggest that folium crataegi could be a valuable source of compounds for enhancing skin regeneration through multiple ways.
研究领域:
药理学
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烧伤治疗
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伤口愈合
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分子生物学
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2024
·
IF 4.4
Xiaoqin Zhang,Jiale Mao,Lu Shao,Shuang Liu,Jiwang Zhou,Mingrong Mei,Zunjing Zhang
Background: Melastoma dodecandrum Lour. (MD), a traditional botanical drug known for its hypoglycemic, antioxidant, and anti-inflammatory properties, is commonly used to treat diabetes, osteoarthritis, and osteoporosis. However, its specific active components against diabetic osteoporosis remain unclear.Purpose: This study aimed to identify the key active components in MD using cell membrane chromatography coupled with mass spectrometry and validate their effects in vitro.Methods: An AGEs-induced osteoblast injury model was established. MTT assays measured cell viability, and ALP activity was assessed using a biochemical kit. Western blotting was employed to detect the expression levels of osteoblast-related proteins OCN and RUNX2 and the AGE receptor protein RAGE. ELISA was used to determine the levels of SOD, MDA, CAT, and GPx. PCR quantified TNF-α expression to evaluate the protective effects and potential mechanisms of MD. The AGEs-induced osteoblast cell membrane chromatography-mass spectrometry method facilitated the rapid identification of potentially active compounds based on their affinity for the osteoblast cell membrane. Cell experiments further validated the activity of the characteristic component isovitexin.Results: MD significantly improved cell viability in AGEs-damaged osteoblasts, enhanced ALP, SOD, CAT, and GPx activities, reduced MDA levels, increased OCN and RUNX2 protein expression, and decreased TNF-α mRNA and RAGE protein expression. Cell membrane chromatography identified 20 chemical constituents, including 13 flavonoids, 4 organic acids, 1 phenylpropanoids, 1 terpenoids, and 1 alkaloid. Cell experiments have confirmed that isovitexin has significant protective activity against osteoblasts and can inhibit the expression of specific receptor RAGE on the osteoblast membrane, consistent with the effect of MD.Conclusion: MD and its active component, isovitexin, provide protective effects against AGEs-induced osteoblast injury, offering a basis for subsequent drug development.
研究领域:
药理学
·
生物化学
·
细胞生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）