COMPOSITES SCIENCE AND TECHNOLOGY
·
2022
·
IF 9.879
Bingjian Deng,Lei Fang,Kuanjun Fang,Xinxin Han,Yueyao Liang
Compared with traditional clothing, wearable textiles with physiotherapy were a breakthrough innovation, among which fiber materials with good mechanical properties and excellent electrical conductivity were key. Herein, we prepared profiled conductive composite fibers (PCFs) with Tai Chi structure based on the polyacrylonitrile (PAN) and carboxylic multiwall carbon nanotubes (MWCNTs) by wet spinning, achieving the synchronous improvement of mechanical and conductivity under a small quantity of conductive fillers doped. By changing the angle of spinning, the migration of MWCNTs could be induced and controlled, realizing the preparation of Tai Chi structure. The PAN and MWCNTs were enriched into the axial unilateral of fibers respectively, similar to the Tai Chi structure, when the spinning angle was 30°. The special Tai Chi structure could concentrate carriers to form a continuous and interpenetrating conductive path and retained the good flexibility and tensile strength of composite fibers. The PCFs prepared with 6 wt% MWCNTs loading possessed 51.6 S/cm and 291 MPa. The wearable electric heaters prepared by PCFs could get a comfortable temperature (30 °C ∼ 60 °C) under the safe voltage condition and had good repeatability and stability, which was expected to provide valuable reference for the development of thermotherapy textiles.
研究领域:
材料科学
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医学工程
·
纺织科学
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产品关联
DOI
高影响因子
Chinese Herbal Medicines
·
2025
·
IF 8.9
Jing He,Zixin Zhao,Ying Ye,Yujia Zhai,Dexun Yu,Fangyuan Gao,Jianlin Wu,Jun Wen,Tingting Zhou
Objective Zhi-zi-chi decoction (ZZCD) is a classical antidepressant traditional Chinese medicine (TCM) formula. However, the microbiological basis of its antidepressant effects remains unclear. The objective of this study was to investigate the microbial basis of ZZCD. Methods The bacteria that exhibit enhanced growth in ZZCD extracts were identified through in vitro culture. A depressed rat model was subsequently established to assess the antidepressant effects of the bacteria. A GC-MS-based metabolomics approach and molecular docking simulation were employed to investigate the potential mechanism of the antidepressant effects of the bacteria from the perspective of changes in the metabolic profile of the gut microbiota. Finally, the antioxidant effects of gut microbial metabolites following Lactobacillus rhamnosus treatment were validated through cellular experimentation. Results ZZCD was able to promote the growth of L. rhamnosus . Furthermore, L. rhamnosus was demonstrated to increase the percentage of sucrose preference, reduce the immobility time in tail suspension and forced swimming tests, and alleviate depressive symptoms in rats. Additionally, L. rhamnosus was shown to alter the metabolic profile of gut microbiota. A total of six metabolites (ethanamin, butyric acid, 2-methylbutanoic acid, behenic acid, 3-methylvaleric acid and 1-hexadecanol) associated with depressive behavior in rats were identified. All six metabolites demonstrated the capacity to bind to Keap1 in molecular docking simulations. Gut microbial metabolites after L. rhamnosus treatment increased Nrf2 levels in HT22 cells. Conclusion L. rhamnosus may be one of the microbial bases for the antidepressant effects of ZZCD. Its effects may be related to the regulation of gut microbiota metabolism and anti-oxidative stress.
研究领域:
中医药学
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微生物学
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神经科学
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代谢组学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2017
·Vol. 6
·期 1
·179-188
·
IF 4.21
Jing Li,Cuiting Zhang,Weiming He,Hongzhi Qiao,Jiahui Chen,KaiKai Wang,David Oupický,Minjie Sun
Kidney-specific delivery is critically important for the treatment of renal fibrosis with drugs such as salvianolic acid B (Sal B). Here we report a kidney-specific nanocomplex formed by the coordination-driven assembly of catechol-modified low molecular weight chitosan (HCA-Chi), calcium ions and Sal B. The prepared HCA-Chi-Ca-Sal B (HChi-Ca-Sal B) nanocomplex reversed the TGF-β1-induced epithelial–mesenchymal transition (EMT) in HK-2 cells. In vivo imaging demonstrated a kidney-specific biodistribution of the nanocomplex. The anti-fibrosis effect of HChi-Ca-Sal B was tested in a mouse model of unilateral ureteral obstruction (UUO). Significant attenuation of the morphological lesions and the levels of extracellular matrix (ECM) proteins in the tubulointerstitium was observed in mice treated with HChi-Ca-Sal B, suggesting that the nanocomplex was able to prevent fibrosis better than the treatment with free Sal B. It was concluded that the HChi-Ca-Sal B nanocomplex showed a specific renal targeting capacity and could be utilized to enhance Sal B delivery for treating renal fibrosis.
研究领域:
药理学
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生物医学工程
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肾脏病学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 21
·3497
·
IF 4.7
Puxi Fan,Xuyu Liang,Yongtao Fei,Wenhong Zhao,Jinglong Liang,Weidong Bai,Songgui He
The distinct flavor of chi-flavor baijiu (CFB) has garnered significant attention in China. After the optimization of fermentation conditions,Pichia anomalaandLactobacillus plantarumwere introduced into the fermentation process to enhance the flavor. Samples inoculated with these mixed strains (SY) exhibited higher levels of alcohol (from 33.04 to 178.55 mg/L) and esters (from 49.51 to 130.20 mg/L) compared to the control group (KB). In SY,P. anomalaandL. plantarumwere the predominant microorganisms, whilePediococcusandSaccharomyceswere more prevalent in KB. Moreover, 68 volatile flavor compounds were detected in SY, as opposed to 64 in KB. Notably,Pichiashowed a positive correlation with key flavor compounds. The synergistic fermentation with exogenous strains led to a 52.38% increase in phenethyl alcohol and a 4.91% increase in ethyl lactate. Additionally, the levels of other flavor compounds, like ethyl acetate, γ-nonanolactone, and (E)-2-octenal, also increased. The results demonstrated that the addition ofP. anomalaandL. plantarumto the fermentation process of CFB significantly increased the contents of flavor compounds. This research reveals valuable insights into flavor enhancement and the microbial community dynamics in CFB production.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Fungal Biology
·
2022
·
IF 2.91
Rong Zhou,Hanbing Yang,Ting Lu,Yanxia Zhao,Weifa Zheng
Production of hispidin polyphenols in Inonotus obliquus is a stress-induced response triggered by environmental factors. As one of the important environmental factors, ultraviolet (UV) radiation plays regulatory roles in fungal growth and development . However, whether UV radiation regulates the formation of hispidin polyphenols remains to be established. In this study, we cultivated I. obliquus on solid medium and imposed intermittent UV radiation. We showed that UV exposure inhibited the growth of mycelia but increased the production of polyphenols. Further bioassays revealed that UV radiation also increased the catalytic activities of phenylalanine ammonia-lyase (PAL) and chalcone isomerase (CHI), up-regulated expression of genes related to redox, transcriptional regulation, and metabolism. In addition, the total extracts from the UV-irradiated group were more capable of scavenging DPPH and ABTS + free radicals, especially at the later stage of culture. Thus, UV radiation, acting as one of the environmental factors, stimulated the accumulation of polyphenols in I. obliquus by regulating the activities of enzymes and the expression of genes related to growth and metabolism, and can be tentatively used as a feasible strategy to enhance the production of polyphenols in I. obliquus .
研究领域:
真菌学
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药理学
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环境生物学
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DOI
PubMed
高影响因子
Materials Today Chemistry
·
2022
·
IF 7.613
G. Zhong,C. Tong,X. Liu,J. Fan,X. Xiong,P. Chen,A. Zhu,R. Yu,B. Liu
The emergence and spreading of methicillin-resistant Staphylococcus aureus ( MRSA ) have become one of the main reasons for the surgical failure even for the death. Thus, there is an urgent need to develop efficient antimicrobial alternatives for eradicating it so as to improve the therapy efficiency of related diseases. Although the synthesized metal nanoparticles showed the MRSA -killing function in vitro , their biosafety in vivo application is still in controversy due to the potential toxicity and induced multidrug resistance. Recently, plant natural compounds with high antibacterial activity and special mechanisms different from antibiotics have attracted wide interest for developing new anti- MRSA regents. In this work, new nanocomplexes of [email protected] @CBD based on natural compounds of cannabidiol (CBD) and chitosan (Chi) were designed for eradicating MRSA . In vitro results demonstrate that [email protected] @CBD NPs plus laser irradiation can efficiently kill MRSA by inducing bacterial surface perforation, content leakage, ROS production, and ATP reduction. In vivo results show that the combination of [email protected] @CBD NPs plus laser can effectively remove bacteria and accelerate wound healing of MRSA -infected normal and diabetes mellitus mice by up-regulating VEGF and CD31. Moreover, [email protected] @CBD NPs possessing excellent biocompatibility and ultra-low toxicity in vitro significantly delayed the time for multidrug-resistance induction, comparing with silver nanoparticles. In our point, the “green therapeutics” against MRSA show great potential in clinical application.
研究领域:
纳米技术
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药学
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微生物学
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生物医学工程
·
糖尿病研究
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DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Yun-Lu Song,Jia-Lin Xing,Charles Ampomah-Dwamena,Shuai-Hui Ma,Mao-Song Pei,Han-Li Liu,Rui-Ping Feng,Yong-Ke Wu,Tong-Lu Wei,Hai-Nan Liu,Da-Long Guo
Grape seed extract (GSE) is a natural polyphenolic compound with potent antioxidant activity. We developed a composite preservation coating material by combining GSE with nano-zinc oxide (Nano-ZnO) and chitosan (CHI), two widely used agents in postharvest preservation. The CHI-GSE-Nano-ZnO coating effectively inhibited postharvest decay and berry abscission while delaying quality deterioration in grapes. Treatment with the composite coating enhanced the activities of antioxidant enzymes (SOD, POD, CAT, and APX) while reducing oxidative stress markers (O 2 - , H 2 O 2 , and malondialdehyde), thereby mitigating ROS accumulation to preserve postharvest grape quality. To elucidate the underlying mechanism, transcriptome sequencing was conducted on ‘Kyoho’ grape samples collected 3, 6, and 9 days post-treatment with CHI, CHI-GSE, and CHI-GSE-Nano-ZnO. Transcriptomic profiling revealed upregulation of VvSTS484 , a pivotal stilbene synthase (STS)-encoding gene, in the CHI-GSE-Nano-ZnO group. Promoter analysis identified multiple MYB-binding cis-elements in VvSTS484 . Yeast one-hybrid and transient dual-luciferase assays demonstrated that the transcription factor VvMYB714 directly binds to four specific MBS motifs in the VvSTS484 promoter (MBS1, -410 to -416 bp; MBS2, -1201 to -1207 bp; MBS3, -1554 to-1560 bp; and MBS4, -1906 to -1912 bp). This molecular interaction activated VvSTS484 expression, leading to enhanced resveratrol biosynthesis that peaked at day 9 post-treatment. The elevated resveratrol level in the CHI-GSE-Nano-ZnO group aligned with reduced oxidative stress and delayed quality deterioration, consistent with its established role in enhancing plant stress tolerance. In conclusion, the CH-GSE-Nano-ZnO composite coating inhibits postharvest decay via the MYB- pSTS module, providing a theoretical basis for developing natural product-based preservation technologies for fruit and vegetables.
研究领域:
采后生理学
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植物生物化学
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食品科学
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分子生物学
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园艺科学
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产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2015
·Vol. 23
·期 13
·17487-17510
·
IF 3.488
Dibo Hou,Jian Zhang,Zheling Yang,Shu Liu,Pingjie Huang,Guangxin Zhang
The issue of distribution water quality security ensuring is recently attracting global attention due to the potential threat from harmful contaminants. The real-time monitoring based on ultraviolet optical sensors is a promising technique. This method is of reagent-free, low maintenance cost, rapid analysis and wide cover range. However, the ultraviolet absorption spectra are of large size and easily interfered. While within the on-site application, there is almost no prior knowledge like spectral characteristics of potential contaminants before determined. Meanwhile, the concept of normal water quality is also varying due to the operating condition. In this paper, a procedure based on multivariate statistical analysis is proposed to detect distribution water quality anomaly based on ultraviolet optical sensors. Firstly, the principal component analysis is employed to capture the main variety features from the spectral matrix and reduce the dimensionality. A new statistical variable is then constructed and used for evaluating the local outlying degree according to the chi-square distribution in the principal component subspace. The possibility of anomaly of the latest observation is calculated by the accumulation of the outlying degrees from the adjacent previous observations. To develop a more reliable anomaly detection procedure, several key parameters are discussed. By utilizing the proposed methods, the distribution water quality anomalies and the optical abnormal changes can be detected. The contaminants intrusion experiment is conducted in a pilot-scale distribution system by injecting phenol solution. The effectiveness of the proposed procedure is finally testified using the experimental spectral data.
研究领域:
环境科学
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水处理工程
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统计学应用
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产品关联
DOI
PubMed
高影响因子
ANALYST
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2022
·Vol. 147
·期 10
·2272-2279
·
IF 5.227
Tao Wen,Chenchen Xia,Qiubo Yu,Yujie Yu,Siyuan Li,Chunli Zhou,Kexin Sun,Song Yue
Cervical cancer is a common cancer in women. HPV16 E6 oncoprotein is a reliable biomarker for cervical cancer. Although there are other methods for detecting E6 oncoprotein, the electrochemical method has more advantages, such as low cost, convenience and speed. In this study, a novel dual-signal electrochemical immunosensor for quick and sensitive detection of E6 oncoprotein based on a high efficiency catalyst and signal label was developed. Herein, to achieve quick detection, palladium–boron–phosphorus dendritic ternary nanospheres (PdBP NSs) not only acted as a catalyst to catalyze H2O2, but also as a support material to capture antibodies. Moreover, to realize sensitive detection, nanocomposites of mesoporous silica nanoparticles loaded with methylene blue and coated with chitosan (MBSi-Chi) were synthesized as a signal label, which can produce electrochemical signal. Under optimal conditions, the label-free immunosensor exhibited a linear range of 100 fg mL−1 to 4 ng mL−1 with a detection limit of 72.8 fg mL−1 (S/N = 3), and the sandwich-type immunosensor presented a linear range of 50 fg mL−1 to 4 ng mL−1 with a detection limit of 34.1 fg mL−1 (S/N = 3). The as-prepared dual-signal immunosensor had desirable specificity, stability and repeatability, implying its potential applications in clinical laboratory.
研究领域:
生物化学
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纳米技术
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分析化学
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生物医学工程
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癌症研究
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2014
·
IF 8.312
Hongzhi Qiao,Minjie Sun,Zhigui Su,Ying Xie,Minglei Chen,Li Zong,Yahan Gao,Huipeng Li,Jianping Qi,Qun Zhao,Xiaochen Gu,Qineng Ping
Renal fibrosis is a common progressive kidney disease, and there is a lack of efficient treatment for the condition. In this study, we designed a kidney-specific nanocomplex by forming coordination-driven assembly from catechol-derived low molecular weight chitosan (HCA-Chi), metal ions and active drug molecules. The coordination activities of various metals and ligands, cytotoxicity, immunogenicity and biodistribution of HCA-Chi were investigated. Autofluorescent doxorubicin (DOX) was selected to fabricate HCA-Chi-Cu-DOX ternary nanocomplex for investigating cellular uptake behavior, transmembrane and targeting properties. The nanodevice demonstrated satisfactory stability under normal physiological conditions and pH-responsive drug release in acidic environments. Uptake of HCA-Chi-Cu-DOX by HK-2 cells was dependent on exposure time, concentration, and temperature, and was inhibited by blockers of megalin receptor. Tissue distribution showed that HCA-Chi-Cu-DOX nanocomplex was specifically accumulated in kidney with a renal relative uptake rate ( r e ) of 25.6. When active anti-fibrosis compound emodin was installed in HCA-Chi-Zn-emodin and intravenously injected to the ureter obstructed mice, obvious attenuation of fibrotic progression was exhibited. It was concluded that HCA-Chi coordination-driven nanocomplex showed special renal targeting capacity and could be utilized to develop drug delivery systems for treating renal fibrosis.
研究领域:
药学
·
纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceuticals
·
2026
·Vol. 19
·期 6
·819
·
IF 5.7
Xue Jiang,Jicheng Yang,Ying Zhang,Yusheng Zhang,Qingqing Li,Shaoqi Song,Zhen Ouyang,Hongjun Yang,Xianyu Li,Luqi Huang
Background:Neuroinflammation and gut–brain axis (GBX) dysregulation are key pathological drivers of stress-related neuropsychiatric disorders. Zhi-Zi-Chi Decoction (ZZCD), a classic Traditional Chinese Medicine (TCM) formula, has been clinically used to alleviate mental disturbances via the TCM principle of “clearing heat and relieving restlessness.” Still, its modern neuroprotective mechanisms, especially its links to gut microbiota and central signaling pathways, remain incompletely elucidated.Purpose:This study aimed to systematically investigate the therapeutic effects of ZZCD on chronic restraint stress (CRS)-induced neurodysfunction in mice and clarify its mechanisms from the perspectives of TCM theory, material basis, gut microbiota–metabolite axis, and central signaling pathways.Method:CRS mice were treated with ZZCD or protocatechuic acid. Behavioral tests evaluated depression- and anxiety-like behaviors. UHPLC-Q-TOF/MS identified ZZCD’s chemical constituents; 16S rRNA sequencing and untargeted metabolomics analyzed gut microbiota and metabolite changes. Western blot, immunofluorescence, and proteomics examined neuroinflammation, microglial polarization, and signaling pathway activity (PI3K/Akt/mTOR, AMPK).Results:ZZCD reversed CRS-induced depression- and anxiety-like behaviors and suppressed neuroinflammation. Mechanistically, UHPLC-Q-TOF/MS identified 424 ZZCD constituents, with prenol lipids, organooxygen compounds, and flavonoids as the most abundant. ZZCD reversed CRS-induced imbalance in gut microbiota, reducing pro-inflammatory Prevotella and enriching beneficial Lactobacillus, and mediated the enrichment of the prebiotic metabolite PCA in colonic and serum samples, which crossed the blood–brain barrier (BBB) to exert neuroprotection. Additionally, ZZCD and PCA normalized the PI3K/Akt/mTOR pathway and activated AMPK, promoting M2 microglial polarization and restoring synaptic plasticity.Conclusions:ZZCD exerts antidepressant effects by a gut-microbiota-dependent modulation of PCA-PI3K/Akt/mTOR and AMPK dual axes that converts microglia from M1 to M2, providing ethnopharmacological evidence and a mechanistic rationale for its clinical application in major depressive disorder.
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Yangfei Ding,Linzi Fan,Jing Zhu,Jiajia Wang,Jiqiao Yuan,Yuanjie Fan,Lin Lv,Manyuan Wang
Ethnopharmacological relevance Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by a high clinical recurrence rate. Hao-Chi-Dan (HCD) is a Chinese herbal formula widely used in the clinical treatment of gastrointestinal pain and bleeding. However, the efficacy of HCD for UC and the impact of processing methods for its key ingredient, Artemisiae Argyi Folium (AAF), on therapeutic outcomes remain unclear. Aim of the study This study aimed to evaluate the efficacy of HCD against UC and to investigate the potential active components and mechanisms of action of HCD formulated with vinegar-processed AAF (VHCD). Materials and methods Chemical profiles of HCD formulated with raw, vinegar-processed, and vinegar-charred AAF were characterized by UPLC-QTOF-MS/MS and HS-GCMS. A dextran sulfate sodium salt (DSS)-induced murine UC model was used to assess the efficacy of different HCD formulations. Disease activity, colon histopathology, inflammatory cytokines, gut microbiota, and short-chain fatty acids (SCFAs) were analyzed. Results HCD significantly alleviated DSS-induced colitis. Crucially, VHCD demonstrated superior efficacy in ameliorating disease activity, colon shortening, and tissue damage, without inducing hepatorenal toxicity. Chemical analysis identified terpenoids and flavonoids as major components. VHCD treatment modulated the gut microbiota structure, increased beneficial SCFAs, and reduced pro-inflammatory cytokines. Conclusion Vinegar-processing is essential for enhancing the anti-colitis efficacy of HCD. The effect is associated with modulation of gut microbiota, increased SCFA production, and attenuated inflammation. Our findings provide a scientific basis for the optimized application of HCD in UC management.
研究领域:
民族药理学
·
中医药
·
植物化学
·
炎症性肠病
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代谢组学
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微生物组研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）